T1 transgenic tobacco plants
... English have helped the researcher have an overview about relating matters as folowing: (1) maize weevils (Sitophilus zeamais Motsch); (2) the attributes/ characters of plant defensines; and (3) Transgenic engineering in maize plants. Maize weevil is a popular destructive species and protopathic pes ...
... English have helped the researcher have an overview about relating matters as folowing: (1) maize weevils (Sitophilus zeamais Motsch); (2) the attributes/ characters of plant defensines; and (3) Transgenic engineering in maize plants. Maize weevil is a popular destructive species and protopathic pes ...
Fulltext PDF - Indian Academy of Sciences
... on DNA sequence. The latter is in the form of cytosine methylation at CG, CHG and CHH elements (where H = A, T or C) and a variety of histone modifications in nucleosomes. The epialleles arising from cytosine methylation marks on the nuclear genomic loci have better heritability than the epiallelic v ...
... on DNA sequence. The latter is in the form of cytosine methylation at CG, CHG and CHH elements (where H = A, T or C) and a variety of histone modifications in nucleosomes. The epialleles arising from cytosine methylation marks on the nuclear genomic loci have better heritability than the epiallelic v ...
Sequence variation in the dihydrofolate reductase
... metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited information exists on the levels of genetic polymorphism of these enzymes in natural populations of any trypanosomatid parasite. ...
... metabolism of protozoan parasites from the family Trypanosomatidae (e.g. Trypanosoma spp ., Leishmania spp. ) that are targets of current drug-design studies. Very limited information exists on the levels of genetic polymorphism of these enzymes in natural populations of any trypanosomatid parasite. ...
MGF 360-17R Missing
... This gene is a fusion between the MGF 110-7L ortholog and MGF 360-6L. The carboxy terminus of this fusion is shown as a small box between the 3L and 4L orthologs as the MGF 110 orthologs are outside of the scope of this diagram. The annotated ortholog for this gene is: “Truncated MGF 360 protein” wh ...
... This gene is a fusion between the MGF 110-7L ortholog and MGF 360-6L. The carboxy terminus of this fusion is shown as a small box between the 3L and 4L orthologs as the MGF 110 orthologs are outside of the scope of this diagram. The annotated ortholog for this gene is: “Truncated MGF 360 protein” wh ...
Dot Plot - APBioNET
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
... Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
Mendelian and Non-Mendelian Regulation of Gene Expression in
... absence expression patterns [34]. Genetical genomics or expression quantitative trait loci (eQTL) mapping is an efficient method for understanding the genetic basis of transcriptome variation [26–27,35–36]. eQTL mapping uses transcript abundance as a phenotypic trait and maps the genomic loci contro ...
... absence expression patterns [34]. Genetical genomics or expression quantitative trait loci (eQTL) mapping is an efficient method for understanding the genetic basis of transcriptome variation [26–27,35–36]. eQTL mapping uses transcript abundance as a phenotypic trait and maps the genomic loci contro ...
Characterization of the unique intron
... acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide linker (3-5). The amino acid sequence of Euglena pLHCPII was ...
... acid sequence and their genes often contain multiple introns. Euglena gracilis, a unicellular protist, has a LHCPII precursor (pLHCPII) that is a polyprotein containing multiple copies of LHCPII covalently joined by a conserved decapeptide linker (3-5). The amino acid sequence of Euglena pLHCPII was ...
Application of whole genome sequencing to fully characterise
... Campylobacter is the most common cause of acute bacterial gastroenteritis worldwide. In the UK alone it causes an estimated 500,000 infections each year. There have been two large studies of Infectious Intestinal Disease in the UK community (IID1 in the mid 1990s and IID2 in 2008-2009). In both stud ...
... Campylobacter is the most common cause of acute bacterial gastroenteritis worldwide. In the UK alone it causes an estimated 500,000 infections each year. There have been two large studies of Infectious Intestinal Disease in the UK community (IID1 in the mid 1990s and IID2 in 2008-2009). In both stud ...
Lecture 3: Sequence Alignment
... Compare the gene eyeless of Drosophila Melanoganster with the human gene aniridia. They are master regulatory genes producing proteins that control large cascade of other genes. Certain segments of genes eyeless of Drosophila melanogaster and human aniridia are almost identical. The most important o ...
... Compare the gene eyeless of Drosophila Melanoganster with the human gene aniridia. They are master regulatory genes producing proteins that control large cascade of other genes. Certain segments of genes eyeless of Drosophila melanogaster and human aniridia are almost identical. The most important o ...
genes - McGraw Hill Higher Education
... Two main themes underlying the observations on chromosomal changes 1. Karyotypes generally remain constant within a species • Most genetic imbalances result in a selective disadvantage ...
... Two main themes underlying the observations on chromosomal changes 1. Karyotypes generally remain constant within a species • Most genetic imbalances result in a selective disadvantage ...
Plant Functional Genomics Plant Functional Genomics
... and E. coli as the host, making library construction and subsequent downstream procedures efficient and easy to perform. Recombinant DNA inserts up to 200 kb can be efficiently cloned and stably maintained in E. coli. Although the insert size cloning capacity is much lower than that of the YAC syste ...
... and E. coli as the host, making library construction and subsequent downstream procedures efficient and easy to perform. Recombinant DNA inserts up to 200 kb can be efficiently cloned and stably maintained in E. coli. Although the insert size cloning capacity is much lower than that of the YAC syste ...
Diploidy and the selective advantage for sexual reproduction in
... The evolution and maintenance of sexual reproduction is regarded as one of the central problems of evolutionary biology (Bell 1982; Williams 1975; Maynard-Smith 1978; Michod 1995; Hurst and Peck 1996; Agrawal 2006; Visser and Elena 2007). The various theories for the selective advantage for sex fall ...
... The evolution and maintenance of sexual reproduction is regarded as one of the central problems of evolutionary biology (Bell 1982; Williams 1975; Maynard-Smith 1978; Michod 1995; Hurst and Peck 1996; Agrawal 2006; Visser and Elena 2007). The various theories for the selective advantage for sex fall ...
PDF
... H3K27me3 mark targets both, genes and repetitive elements and is anti-correlated with transcriptional activity. We show that H3K27me3 distribution over its targets is not uniform, but can be grouped into several different clusters that correlate with different levels of repression. Furthermore, we d ...
... H3K27me3 mark targets both, genes and repetitive elements and is anti-correlated with transcriptional activity. We show that H3K27me3 distribution over its targets is not uniform, but can be grouped into several different clusters that correlate with different levels of repression. Furthermore, we d ...
Structure, expression and chromosomal location of the Oct
... uncloned "gap" between Q and T. In situ analysis of early preimplantation embryos reveals that Oct-4 is expressed both maternally and zygotically. Surprisingly, zygotic expression is not detectable until the 8-cell stage when compaction begins. In addition to Oct-4, several Oct-4-related genes are p ...
... uncloned "gap" between Q and T. In situ analysis of early preimplantation embryos reveals that Oct-4 is expressed both maternally and zygotically. Surprisingly, zygotic expression is not detectable until the 8-cell stage when compaction begins. In addition to Oct-4, several Oct-4-related genes are p ...
The RNA world meets behavior: AfiI pre
... RNA editing and indicate several areas for future research. First, determining the precise effects of any particular RNA editing site will be of interest and might be aided by a combined evolutionary approach. Second, identifying potential ADAR targets will certainly require more detail about what A ...
... RNA editing and indicate several areas for future research. First, determining the precise effects of any particular RNA editing site will be of interest and might be aided by a combined evolutionary approach. Second, identifying potential ADAR targets will certainly require more detail about what A ...
exon junctions of Euglena gene(s) - DigitalCommons@University of
... containing 7.4 kb of the 3' end of a LHCPII gene (4). Nine exons were identified in this clone based on sequence homology with Arabidopsis LHCPII. They can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of an LHCPII, followed by 4 complete LHCPIIs (4). I ...
... containing 7.4 kb of the 3' end of a LHCPII gene (4). Nine exons were identified in this clone based on sequence homology with Arabidopsis LHCPII. They can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of an LHCPII, followed by 4 complete LHCPIIs (4). I ...
RNA Splicing
... • In other cases, a single primary transcript is spliced in more than one way, and internal exons are substituted, added, or deleted. • In some cases, the multiple products all are made in the same cell, but in others the process is regulated so that particular splicing patterns occur only under pa ...
... • In other cases, a single primary transcript is spliced in more than one way, and internal exons are substituted, added, or deleted. • In some cases, the multiple products all are made in the same cell, but in others the process is regulated so that particular splicing patterns occur only under pa ...
Application Note: Targeted sequencing and chromosomal haplotype
... Each individual TLA template contains fragments that may be ten to hundreds of kilobases apart in base space, but are all close enough to be cross-linked. Because it is not a targeted approach, this strategy results in long range information that covers the entire genome. ...
... Each individual TLA template contains fragments that may be ten to hundreds of kilobases apart in base space, but are all close enough to be cross-linked. Because it is not a targeted approach, this strategy results in long range information that covers the entire genome. ...
Proviral amplification of the Gypsy endogenous retrovirus of
... (A) Organization of the gypsy element and the Act-env construct. Numbers refer to gypsy nucleotides (Marlor et al., 1986). Boxes correspond to ORFs. Two long terminal repeats (LTRs), shown by right-pointing arrowheads, flank a central region which contains the three ORFs corresponding to gag, pol an ...
... (A) Organization of the gypsy element and the Act-env construct. Numbers refer to gypsy nucleotides (Marlor et al., 1986). Boxes correspond to ORFs. Two long terminal repeats (LTRs), shown by right-pointing arrowheads, flank a central region which contains the three ORFs corresponding to gag, pol an ...
Analysis and nucleotide sequence of an origin of DNA replication in
... Restreaking on minimal plates with Ap yielded 16 candidates. From these the plasmid DNA was prepared from 3 ml overnight cultures and retransformed to A. calcoaceticus BD413 trpE. The transformants were plated on LB-Ap plates and minimal medium Ap plates. Four plasmids yielded roughly the same numbe ...
... Restreaking on minimal plates with Ap yielded 16 candidates. From these the plasmid DNA was prepared from 3 ml overnight cultures and retransformed to A. calcoaceticus BD413 trpE. The transformants were plated on LB-Ap plates and minimal medium Ap plates. Four plasmids yielded roughly the same numbe ...
Distortion of quantitative genomic and expression
... and repeated twice. The hybridized genomic sequences ...
... and repeated twice. The hybridized genomic sequences ...
Genome Visualisation and Annotation Tools: Artemis and ACT
... DNA within a genome, many of which will have been laterally acquired. Since it has been shown in many bacteria that laterally acquired DNA carries important genes for virulence and lifestyle it is worth spending a little time exploring some of these regions. You should identify a region which you fe ...
... DNA within a genome, many of which will have been laterally acquired. Since it has been shown in many bacteria that laterally acquired DNA carries important genes for virulence and lifestyle it is worth spending a little time exploring some of these regions. You should identify a region which you fe ...
Molecular Biology of Transcription and RNA Processing
... In 1957, microbiologist Elliot Volkin and geneticist Lazarus Astrachan used the pulse-chase method to examine transcription in bacteria immediately following infection by a bacteriophage. Exposing newly infected bacteria to radioactive uracil, they observed rapid incorporation of the label, indicati ...
... In 1957, microbiologist Elliot Volkin and geneticist Lazarus Astrachan used the pulse-chase method to examine transcription in bacteria immediately following infection by a bacteriophage. Exposing newly infected bacteria to radioactive uracil, they observed rapid incorporation of the label, indicati ...
Multiple Roles of the Y Chromosome in the Biology of
... segregates from the X chromosome, irrespective of its origin”[15]. This suggests two considerations: (1) although all Y chromosomes seem to evolve similarly in many organisms once they have been established, their origin may be very different even in the same taxon[15,38], being derived either from ...
... segregates from the X chromosome, irrespective of its origin”[15]. This suggests two considerations: (1) although all Y chromosomes seem to evolve similarly in many organisms once they have been established, their origin may be very different even in the same taxon[15,38], being derived either from ...
Translational selection is operative for synonymous codon usage in
... 2000a). Finally, a ‘common theme’ in completely sequenced genomes is the finding of regions displaying base compositions far away from those of the genome as a whole. These regions have been interpreted as being the result of events of horizontal transfer of DNA between species differing in their ge ...
... 2000a). Finally, a ‘common theme’ in completely sequenced genomes is the finding of regions displaying base compositions far away from those of the genome as a whole. These regions have been interpreted as being the result of events of horizontal transfer of DNA between species differing in their ge ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.