Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we found that DNA molecules from P22pro-I and P22pro-3 each contain a substituti ...
... that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we found that DNA molecules from P22pro-I and P22pro-3 each contain a substituti ...
Developing expressed sequence tag libraries and
... amplifying a product of which 17 (71 %) detected polymorphisms in R. idaeus. These results indicate that markers that amplify a polymorphic product in highlyhomozygous black raspberry are likely to amplify a polymorphic product in red raspberry, regardless of the sequence source. ...
... amplifying a product of which 17 (71 %) detected polymorphisms in R. idaeus. These results indicate that markers that amplify a polymorphic product in highlyhomozygous black raspberry are likely to amplify a polymorphic product in red raspberry, regardless of the sequence source. ...
Small Nucleolar RNA
... • Antisense elements dictate where the modifications are catalyzed Filipowicz et al. 2002 ...
... • Antisense elements dictate where the modifications are catalyzed Filipowicz et al. 2002 ...
Sequence Heterogeneities Among 16s
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
Pourcel et al., Microbiology 2005
... The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or ‘spacers’. CRISPRs are present in both archaea and bacteria, in association with genes involved in DNA recombination and repair ...
... The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or ‘spacers’. CRISPRs are present in both archaea and bacteria, in association with genes involved in DNA recombination and repair ...
Journal of Biotechnology
... The 37.2 kb bmy gene cluster is an insertion within the FZB42 genome, which is separated by only 25 kb from the fengycin gene cluster. It comprises four genes (bmyD, bmyA, bmyB and bmyC) without orthologues in B. subtilis 168 (Fig. 3). The first gene bmyD encodes a putative malonyl coenzyme A transac ...
... The 37.2 kb bmy gene cluster is an insertion within the FZB42 genome, which is separated by only 25 kb from the fengycin gene cluster. It comprises four genes (bmyD, bmyA, bmyB and bmyC) without orthologues in B. subtilis 168 (Fig. 3). The first gene bmyD encodes a putative malonyl coenzyme A transac ...
Cloning and functional expression of invertebrate connexins from
... it should be noted that the reported sequence identity between these two families is very low [7]. Innexins display only 16% overall identity when their full-length amino acid sequences are compared to either connexins or pannexins, which may simply reflect that all three gene families encode four t ...
... it should be noted that the reported sequence identity between these two families is very low [7]. Innexins display only 16% overall identity when their full-length amino acid sequences are compared to either connexins or pannexins, which may simply reflect that all three gene families encode four t ...
- University of Bath Opus
... similarity in sex-biased gene expression among neighbouring genes. Whether this clustering of genes with similar expression profiles is functional or instead the result of transcriptional interference with adjacent genes displaying non-functional but significant similarity in patterns of gene expres ...
... similarity in sex-biased gene expression among neighbouring genes. Whether this clustering of genes with similar expression profiles is functional or instead the result of transcriptional interference with adjacent genes displaying non-functional but significant similarity in patterns of gene expres ...
Brand, Veronica - Degenerate Primer Design using Computational Tools
... degeneracy that does not exceed a predefined limit” (2005). A degenerate primer in this case is a primer where multiple nucleotide bases are possible for a given position in the primer. A primer’s total degeneracy, then, is the total number of specific primers that a degenerate sequence can have, or ...
... degeneracy that does not exceed a predefined limit” (2005). A degenerate primer in this case is a primer where multiple nucleotide bases are possible for a given position in the primer. A primer’s total degeneracy, then, is the total number of specific primers that a degenerate sequence can have, or ...
Chapter 3
... Historically, metazoan animals were subdivided into diploblasts and triploblasts. Diploblasts have two germ layers, the endoderm and the ectoderm, while the triploblasts have a well-defined third germ layer, the mesoderm. This division is based upon the so-called germ layer concept, which has been a ...
... Historically, metazoan animals were subdivided into diploblasts and triploblasts. Diploblasts have two germ layers, the endoderm and the ectoderm, while the triploblasts have a well-defined third germ layer, the mesoderm. This division is based upon the so-called germ layer concept, which has been a ...
An Introduction to RNA Interference (RNAi)
... double-stranded DNA helix that makes up our genome, only that DNA is replaced by its chemical cousin, RNA. While Fire and Mello’s work represented a major advance in understanding the mechanism of RNAi, it also provided for a simple and reproducible method by which long dsRNAs could be used to induc ...
... double-stranded DNA helix that makes up our genome, only that DNA is replaced by its chemical cousin, RNA. While Fire and Mello’s work represented a major advance in understanding the mechanism of RNAi, it also provided for a simple and reproducible method by which long dsRNAs could be used to induc ...
Targeted gene expression as a means of altering cell fates and
... 1. pUAST: we constructed a vector into which genes can be subcloned behind the GAL UAS. A fragment containing five optimized GAL4 binding sites (the ‘ScaI site’ 17-mer; Webster et al., 1988) and a synthetic TATA box (Lillie and Green, 1989) separated by a unique NotI site from the SV40 terminator wa ...
... 1. pUAST: we constructed a vector into which genes can be subcloned behind the GAL UAS. A fragment containing five optimized GAL4 binding sites (the ‘ScaI site’ 17-mer; Webster et al., 1988) and a synthetic TATA box (Lillie and Green, 1989) separated by a unique NotI site from the SV40 terminator wa ...
Identification of novel endogenous antisense transcripts by DNA
... either poly(A)+ or total RNA (data not shown), but quantitative RT-PCR, ISH and microarray analyses were able to detect this transcript within the testis and kidney (see Additional file 5). This result implies that NATs detected by microarray analysis using AFAS probes are transcribed in vivo. We al ...
... either poly(A)+ or total RNA (data not shown), but quantitative RT-PCR, ISH and microarray analyses were able to detect this transcript within the testis and kidney (see Additional file 5). This result implies that NATs detected by microarray analysis using AFAS probes are transcribed in vivo. We al ...
PDF
... Integration of a Selectable Marker into Internal and Subtelomeric Chromosomal var Loci To acquire accurate data regarding transcriptional regulation, it is preferable to study a var promoter within its chromosomal context that can be both transcriptionally silenced and activated. For this purpose we ...
... Integration of a Selectable Marker into Internal and Subtelomeric Chromosomal var Loci To acquire accurate data regarding transcriptional regulation, it is preferable to study a var promoter within its chromosomal context that can be both transcriptionally silenced and activated. For this purpose we ...
An rpoB signature sequence provides unique resolution for the
... The use of morphological characters for the classification of cyanobacteria has often led to ambiguous strain assignment. In the past two decades, the availability of sequences, such as those of the 16S rRNA, nif, cpc and rpoC1 genes, and the use of metagenomics, has steadily increased and has made ...
... The use of morphological characters for the classification of cyanobacteria has often led to ambiguous strain assignment. In the past two decades, the availability of sequences, such as those of the 16S rRNA, nif, cpc and rpoC1 genes, and the use of metagenomics, has steadily increased and has made ...
The tightly regulated promoter of the xanA gene of
... we have shown that the one from Neurospora crassa fully complements a xanA deletion (Cultrone et al., 2005). In this article we investigate whether this gene is subject to the same regulatory signals as all other enzymes of the purine degradation pathway. We observed that the promoter element of xan ...
... we have shown that the one from Neurospora crassa fully complements a xanA deletion (Cultrone et al., 2005). In this article we investigate whether this gene is subject to the same regulatory signals as all other enzymes of the purine degradation pathway. We observed that the promoter element of xan ...
DNA cytosine methylation in plant development
... protect genomes against both endogenous selfish DNA elements (predominantly transposable elements or TEs) and exogenous virus invasions (Yoder et al., 1997; Zilberman, 2008). Indeed, TEs (including both DNA transposons and retrotransposons) are usually heavily methylated and loss of methylation at t ...
... protect genomes against both endogenous selfish DNA elements (predominantly transposable elements or TEs) and exogenous virus invasions (Yoder et al., 1997; Zilberman, 2008). Indeed, TEs (including both DNA transposons and retrotransposons) are usually heavily methylated and loss of methylation at t ...
Conjugative plasmids: vessels of the communal gene pool
... which enable insertion of elements such as gene cassettes into integrons by site-specific recombination, and resolvases, which are DNA endonucleases capable of resolving Holiday junctions that arise as a result of genetic recombinations. Many of these enzymes are encoded by an assortment of selfish ...
... which enable insertion of elements such as gene cassettes into integrons by site-specific recombination, and resolvases, which are DNA endonucleases capable of resolving Holiday junctions that arise as a result of genetic recombinations. Many of these enzymes are encoded by an assortment of selfish ...
Visualization, description and analysis of the Drosophila melanogaster
... A mutation is an adaptively non-directed change in the genomic sequence of an individual, and mutations in the DNA molecule are the ultimate source of genetic variation. Once a new variant appears by mutation in the DNA it can be replicated and transmitted from generation to generation. Gel electrop ...
... A mutation is an adaptively non-directed change in the genomic sequence of an individual, and mutations in the DNA molecule are the ultimate source of genetic variation. Once a new variant appears by mutation in the DNA it can be replicated and transmitted from generation to generation. Gel electrop ...
The amphioxus hairy family: differential fate after duplication.
... but in contrast to its paralog, neither in segmented somites nor in the PSM. Within the CNS, her9 is predominantly expressed in the fore- and midbrain, and transiently in the hindbrain, leaving a non-expressing gap at the midbrain-hindbrain boundary (MHB), and it is also expressed in the midline mes ...
... but in contrast to its paralog, neither in segmented somites nor in the PSM. Within the CNS, her9 is predominantly expressed in the fore- and midbrain, and transiently in the hindbrain, leaving a non-expressing gap at the midbrain-hindbrain boundary (MHB), and it is also expressed in the midline mes ...
The Mean Between Meme and Gene Comparison
... origin of memes paralleling the creation of eukaryotic cells, which paved the way for multicellular life. However, not only are both stories vague and that of the meme’s pure speculation, but memes appear to have evolved from genes themselves rather than being an analogue. In Dennett’s view, memes i ...
... origin of memes paralleling the creation of eukaryotic cells, which paved the way for multicellular life. However, not only are both stories vague and that of the meme’s pure speculation, but memes appear to have evolved from genes themselves rather than being an analogue. In Dennett’s view, memes i ...
Gene Section USF1 (upstream transcription factor 1) Atlas of Genetics and Cytogenetics
... distribution of E-box like elements in the genome. Whole-genome ChIP analysis in HepG2 cells identified 2518 USF1 binding sites in chromatin context, of which 41 % were located within 1 kb of a transcription start site (Rade-Iglesias et al., 2008). USF1 binding signals strongly correlate with target ...
... distribution of E-box like elements in the genome. Whole-genome ChIP analysis in HepG2 cells identified 2518 USF1 binding sites in chromatin context, of which 41 % were located within 1 kb of a transcription start site (Rade-Iglesias et al., 2008). USF1 binding signals strongly correlate with target ...
Mechanisms and impact of genetic recombination in the evolution of
... shared and conserved across all members of the species under consideration. Homologous recombination also occurs between mobile genetic elements (MGE) such as insertion sequences (IS), integrons, bacteriophages, plasmids and transposons, considered being part of the accessory genome (non-core genome ...
... shared and conserved across all members of the species under consideration. Homologous recombination also occurs between mobile genetic elements (MGE) such as insertion sequences (IS), integrons, bacteriophages, plasmids and transposons, considered being part of the accessory genome (non-core genome ...
NIH Public Access
... the inner cell mass and embryo proper is random (reviewed in (Payer and Lee, 2008). Although the timing may differ slightly between human and mouse, this random XCI initially occurs as cells of the inner cell mass begin to differentiate to specific lineages, and involves mechanisms that specify coun ...
... the inner cell mass and embryo proper is random (reviewed in (Payer and Lee, 2008). Although the timing may differ slightly between human and mouse, this random XCI initially occurs as cells of the inner cell mass begin to differentiate to specific lineages, and involves mechanisms that specify coun ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.