The Human Genome.
... from its chimpanzee ortholog by only two amino acid substitutions; nearly one third of human genes have exactly the same protein translation as their chimpanzee orthologs. A major difference between the two genomes is human chromosome 2, which is the product of a fusion between chimpanzee chromosome ...
... from its chimpanzee ortholog by only two amino acid substitutions; nearly one third of human genes have exactly the same protein translation as their chimpanzee orthologs. A major difference between the two genomes is human chromosome 2, which is the product of a fusion between chimpanzee chromosome ...
Document
... • What is a well-known shorthand in one research community is gibberish in another. Contributions by one research community may not be recognized by others. • Without coordination, research work may be duplicated. • The goal of the Gene Ontology Consortium is to produce a controlled vocabulary that ...
... • What is a well-known shorthand in one research community is gibberish in another. Contributions by one research community may not be recognized by others. • Without coordination, research work may be duplicated. • The goal of the Gene Ontology Consortium is to produce a controlled vocabulary that ...
lab_july26_delong - C-MORE
... Softberry FGENESB annotation “pipeline”. http://softberry.com/berry.phtml STEP 1. Finds all potential ribosomal RNA genes using BLAST against bacterial and/or archaeal rRNA databases, and masks detected rRNA genes. STEP 2. Predicts tRNA genes using tRNAscan-SE program (Washington University) and ma ...
... Softberry FGENESB annotation “pipeline”. http://softberry.com/berry.phtml STEP 1. Finds all potential ribosomal RNA genes using BLAST against bacterial and/or archaeal rRNA databases, and masks detected rRNA genes. STEP 2. Predicts tRNA genes using tRNAscan-SE program (Washington University) and ma ...
Clinical Next Generation Sequencing (From Bench to Clinitions)
... (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA. ...
... (NGS) allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include the exome (the protein-coding portion of the genome), specific genes of interest (custom content), targets within genes, or mitochondrial DNA. ...
Milestone1
... Proteins are linear chains of amino acid residues that fold into complex 3D structures that carry out cellular functions. Proteins that have similar linear sequences of amino acid residues often fold into similar 3D shapes and have similar functions. In lecture, we discussed the term “homology” in t ...
... Proteins are linear chains of amino acid residues that fold into complex 3D structures that carry out cellular functions. Proteins that have similar linear sequences of amino acid residues often fold into similar 3D shapes and have similar functions. In lecture, we discussed the term “homology” in t ...
Use of genomic tools
... 3’ untranslated region; identify the transcription start site and the start of the coding region. Mark these features on the genomic sequence. 5- Use BLAST against all genomes to identify homologs using the mRNA sequences (why use the mRNA sequence?). 6- Repeat the same exercise using the protein se ...
... 3’ untranslated region; identify the transcription start site and the start of the coding region. Mark these features on the genomic sequence. 5- Use BLAST against all genomes to identify homologs using the mRNA sequences (why use the mRNA sequence?). 6- Repeat the same exercise using the protein se ...
Eukaryotic Transcription
... – RNA Polymerase I: synthesis of pre-rRNA, which is processed into 28S, 5.8S, and 18S rRNAs – RNA polymerase III: synthesis of tRNA, 18 S rRNA, and small, stable RNAs – RNA polymerase II: synthesis of mRNAs and four small nuclear RNAs that take part in RNA splicing ...
... – RNA Polymerase I: synthesis of pre-rRNA, which is processed into 28S, 5.8S, and 18S rRNAs – RNA polymerase III: synthesis of tRNA, 18 S rRNA, and small, stable RNAs – RNA polymerase II: synthesis of mRNAs and four small nuclear RNAs that take part in RNA splicing ...
Study Guide - final exam
... Experimental Goal: To determine whether 3 different mutations in the essential yeast SPP382 gene had functional consequences on yeast growth. Experimental Design: Transform a functional copy of SPP382 on a URA3-based plasmid (URA3-SPP382(N19)) and different SPP382 alleles (or an empty vector) on a L ...
... Experimental Goal: To determine whether 3 different mutations in the essential yeast SPP382 gene had functional consequences on yeast growth. Experimental Design: Transform a functional copy of SPP382 on a URA3-based plasmid (URA3-SPP382(N19)) and different SPP382 alleles (or an empty vector) on a L ...
An Investigation into the Genomic Evolution of the Histone Gene
... conversion - have been documented to occur, and are understood in molecular detail, but their role in concerted evolution is primarily based on theoretical and/or mathematical models with limited data from actual genome sequence to support them. It is the hypothesis of this research that if unequal ...
... conversion - have been documented to occur, and are understood in molecular detail, but their role in concerted evolution is primarily based on theoretical and/or mathematical models with limited data from actual genome sequence to support them. It is the hypothesis of this research that if unequal ...
DNA Transcription All#read
... one gene may be noncoding for other genes within the same chromosome. ...
... one gene may be noncoding for other genes within the same chromosome. ...
Supplemental Appendix A: ClueGene Algorithm and Time
... Dividing by Ngd corrects for the number of clusters a gene appears in. Without this correction, high scores could be assigned to genes that are “central” in the coexpression network simply because they appear in several clusters. Note that one might also consider including an additional normalizatio ...
... Dividing by Ngd corrects for the number of clusters a gene appears in. Without this correction, high scores could be assigned to genes that are “central” in the coexpression network simply because they appear in several clusters. Note that one might also consider including an additional normalizatio ...
Sequencing genomes
... A new species of frog has been introduced into an area where it has too few natural predators. In an attempt to restore the ecological balance, a team of scientists is considering introducing a species of bird which feeds on this frog. Experimental data suggests that the population of frogs and bird ...
... A new species of frog has been introduced into an area where it has too few natural predators. In an attempt to restore the ecological balance, a team of scientists is considering introducing a species of bird which feeds on this frog. Experimental data suggests that the population of frogs and bird ...
2005 Final Report ( format)
... rampant influence of horizontal gene transfer on the evolution of organisms. Scientists have long been using the phenomenon in recombinant biological studies using prokaryotes. However, recent examination of phylogenies and genome characteristics has shown that horizontal gene transfer (HGT) occurs ...
... rampant influence of horizontal gene transfer on the evolution of organisms. Scientists have long been using the phenomenon in recombinant biological studies using prokaryotes. However, recent examination of phylogenies and genome characteristics has shown that horizontal gene transfer (HGT) occurs ...
... i) Determine the change in the DNA sequence and the resultant change in protein sequence. You will need to consult the known DNA/protein sequence of HIV protease (see lecture notes) to determine the correct reading frame. ii) Does this mutation affect the substrate specificity of HIV protease (the t ...
Document
... happened to have a VNTR in common. Therefore, the 50% estimate of matching bands in a DNA fingerprint based on genetic relationships is a minimum estimate. The value can be higher than that. E22. The minimum percentage of matching bands is based on the genetic relationships. A. 50% B. 50% (on averag ...
... happened to have a VNTR in common. Therefore, the 50% estimate of matching bands in a DNA fingerprint based on genetic relationships is a minimum estimate. The value can be higher than that. E22. The minimum percentage of matching bands is based on the genetic relationships. A. 50% B. 50% (on averag ...
MCB5472_Lecture_2_Feb-3-14
... several sequencing platforms (PacBio, MiSeq) – These especially (but not exclusively) target bacterial genomes where they are most effective ...
... several sequencing platforms (PacBio, MiSeq) – These especially (but not exclusively) target bacterial genomes where they are most effective ...
Slide 2
... acid), molecule that is organized in discrete units called chromosomes. Chromosomes occur in pairs, each member of the pair is inherited from each parent. The process of Meiosis is fundamental to understand how characters are segregated. Every cell of the organism has 2 pairs of each chromosome. How ...
... acid), molecule that is organized in discrete units called chromosomes. Chromosomes occur in pairs, each member of the pair is inherited from each parent. The process of Meiosis is fundamental to understand how characters are segregated. Every cell of the organism has 2 pairs of each chromosome. How ...
how snps help researchers find the genetic
... Consider this: if each of the DNA molecules in our genome was about the size of a ping pong ball, the long unraveled chain of molecules would circle the earth 3 times, or just over 75,000 miles. The real difficulty is that less than 2 percent of that -- about 1500 miles, or a little less than the di ...
... Consider this: if each of the DNA molecules in our genome was about the size of a ping pong ball, the long unraveled chain of molecules would circle the earth 3 times, or just over 75,000 miles. The real difficulty is that less than 2 percent of that -- about 1500 miles, or a little less than the di ...
Pharmacogenomics
... altered expression in a particular disease by comparing the pattern to genes know to be affiliated with a disease. • It can also find genes that fit a certain pattern such as a particular pattern of change with time. • It can also characterize broad functional classes of new genes from the known cla ...
... altered expression in a particular disease by comparing the pattern to genes know to be affiliated with a disease. • It can also find genes that fit a certain pattern such as a particular pattern of change with time. • It can also characterize broad functional classes of new genes from the known cla ...
Using Molecular Markers in Plant Genetics
... variation to locate nearby genes and follow specific forms of those genes through product development,” says Dr. Jim Register, research coordinator of analytical nucleic acid technologies at Pioneer. “SSR markers are considered highly polymorphic as the number of repeats can vary greatly among plant ...
... variation to locate nearby genes and follow specific forms of those genes through product development,” says Dr. Jim Register, research coordinator of analytical nucleic acid technologies at Pioneer. “SSR markers are considered highly polymorphic as the number of repeats can vary greatly among plant ...
Evolution of Plant Genomes Narrative
... The Gene-based Evolution of Duplicated Genes If duplications are a major signature of plant genomes, then the number of genes should correspond to the number of rounds of duplication. Table 1 list the number of genes found within each of the species for which a complete genome sequence is currently ...
... The Gene-based Evolution of Duplicated Genes If duplications are a major signature of plant genomes, then the number of genes should correspond to the number of rounds of duplication. Table 1 list the number of genes found within each of the species for which a complete genome sequence is currently ...
Chem 465 Biochemistry II Hour Exam 3
... and the genes for the transposase that does the transposition. In a complex transposon some additional gene are carried with the ‘jumping’ element. In a direct transposition the DNA simply moves from one site to another. In a replicative transposition one copy remains at the original site while a se ...
... and the genes for the transposase that does the transposition. In a complex transposon some additional gene are carried with the ‘jumping’ element. In a direct transposition the DNA simply moves from one site to another. In a replicative transposition one copy remains at the original site while a se ...
Document
... sequence of DNA bases. Bacterial Artificial Chromosome (BAC)- A vector used to clone DNA fragments of 100 to 300 kb insert size (average of 150 kb) in Escherichia coli cells. Based on the naturally occurring F-factor plasmid found in the bacterium Escherichia coli. Functional Genomic- The study of g ...
... sequence of DNA bases. Bacterial Artificial Chromosome (BAC)- A vector used to clone DNA fragments of 100 to 300 kb insert size (average of 150 kb) in Escherichia coli cells. Based on the naturally occurring F-factor plasmid found in the bacterium Escherichia coli. Functional Genomic- The study of g ...
Gen660_Lecture1B_sequencing_2014
... Use a ‘super matrix’ of variable sequence characters 2. Construct many separate trees, one for each gene, and then compare Often construct a ‘super tree’ that is built from all single trees 3. Incorporate non-sequence characters like synteny, intron structure, etc. ...
... Use a ‘super matrix’ of variable sequence characters 2. Construct many separate trees, one for each gene, and then compare Often construct a ‘super tree’ that is built from all single trees 3. Incorporate non-sequence characters like synteny, intron structure, etc. ...
Homeotic genes
... same as the order in which they appear along the body. In other words, the genes on the left control patterning in the head, and the genes on the right control patterning in the tail. ...
... same as the order in which they appear along the body. In other words, the genes on the left control patterning in the head, and the genes on the right control patterning in the tail. ...
Transposable element
A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.