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I = -[1/3*log 2 (1/3)+ 1/3*log 2 (1/3)+ 1/3*log 2 (1/3)] + 4.32 = 2.73
I = -[1/3*log 2 (1/3)+ 1/3*log 2 (1/3)+ 1/3*log 2 (1/3)] + 4.32 = 2.73

... • Handle introns in RNA/DNA alignments • Data for more that 30 genomes (human, mouse, rat…) ...
Tutorial 3 – Searching the Chinese Hamster
Tutorial 3 – Searching the Chinese Hamster

... The CHO-K1 genome database can be searched by Accession number (i.e. EGV99227) The database can be searched using the GenBank WGS protein accession IDs. For the CHO-K1 WGS project, these accession numbers are EGVXXXXX or EGWXXXXX. These accession numbers can also be found in the NCBI protein databas ...
Document
Document

... 6 Small Subunits (SSU), 12-18 kDa. RbcS gene Encoded in nuclear genome as gene family Synthesized as precursor, 20 Kda, with plastid transit sequence Transported to chloroplasts Possibly for regulation and assembly ...
Sex Chromosomal Transposable Element Accumulation
Sex Chromosomal Transposable Element Accumulation

... al. 1996; Kapitanov and Jurka 1996; Mighell, Markham, and Robinson 1997). The numbers of transitional and transversional differences from the consensus sequence of each Alu class were counted, and divergences were corrected for multiple substitutions (Kimura 1980). To allow autosomal comparison, 172 ...
Chapter 25
Chapter 25

... - The constitutive genes have GC box (GGGCGG consensus sequence) in their promoters - The structural genes have TATA box (TATATAATA sequence) in their promoters. - are located-25 to -30 on the DNA template strand. • RNAPII promoters: - are located at downstream, +40 to +80 on the DNA template strand ...
Development & Evolution ppt
Development & Evolution ppt

... regulatory genes [genes that code for ‘transcription factors’ which control the expression of other genes] Major advances in testing this idea came from using mutant phenotypes in the fruit fly (Drosophila) and the round worm (Caenorhabditis) to ‘dissect’ embryos of these organisms. Studies of homeo ...
Post-transcriptional modifications Cap a
Post-transcriptional modifications Cap a

... Gene silencing is probably often the result of more than one mechanism. Transcriptional gene silencing (TGS) is often associated with methylation of the gene, which may inhibit transcription. In posttranscriptional gene silencing (PTGS), high levels of normal mRNA can cause activation of RNA-depende ...
Slide
Slide

... E-families diverge slowly, but persist for a long periods of time, thus diverging further than the paralogs in N-families N-families undergoes a more dynamic evolution: many duplicate get fixated, many other become pseudogenes. Level of sequence divergence is significantly lower. Duplicate in E-fami ...
4.Genetechnology2
4.Genetechnology2

... that do take up DNA molecules, only a very small proportion will contain the recombinant plasmid with the desired gene ...
En/Spm-LIKE TRANSPOSONS IN POACEAE SPECIES
En/Spm-LIKE TRANSPOSONS IN POACEAE SPECIES

... a given cereal’s nuclear genome) [1]. From the investigation of the Drosophila genomes, it is known that TE can give rise to significant genomic changes by creating mutations, altering gene expression, conducting telomeric function, and promoting chromosomal aberrations [2-4]. TE was divided into tw ...
Chapter 13 – Genetic Mapping of Mendelian Characters
Chapter 13 – Genetic Mapping of Mendelian Characters

... Population association studies • Linkage disequilibrium • Combination of alleles at two closely ...
Identification of Copy Number Variants using genome graphs.
Identification of Copy Number Variants using genome graphs.

... The larger the event, the stronger the signature. However, they are not able to accurately identify smaller events that PEM signatures, even with low coverage, are able to detect. ...
4132010
4132010

... but in lower animal or plants, RNAi effects can be inherited for one or two generations. ...
laboratory of developmental genetics and genetic analysis
laboratory of developmental genetics and genetic analysis

... Our studies are focused on gammaCop, CG6199, As, ARP-like and CG6664 genes which are structural orthologs of human genes associated with the above mentioned syndromes. All of these genes are located on the third Drosophila chromosome and we are currently using reverse genetics in order to analyse th ...
Cloning and expression of chromosomally and plasmid
Cloning and expression of chromosomally and plasmid

... T h e two cfxG genes of A. eutrophus H 1 6 do not have promoters that are active m E colt. However, their ribosome-bmding sites seem to be recogmzed by the foreign host as in the case of the o t h e r cfx genes [2]. T h e i r location relative to cfxP resembles that of prkB and gapB m P~ sphaerotdes ...
Chapter 12
Chapter 12

... § Plasmid DNA is treated with restriction enzyme that cuts in one place, opening the circle § DNA with the target gene is treated with the same enzyme and many fragments are produced § Plasmid and target DNA are mixed and associate with each other § Recombinant DNA molecules are produced when DNA li ...
Clone
Clone

... modified to carry new genes • Plasmids useful as cloning vectors must have • a replicator (origin of replication) • a selectable marker (antibiotic resistance gene) • a cloning site (site where insertion of foreign DNA will not disrupt replication or inactivate ...
Tutorial - Ensembl
Tutorial - Ensembl

... The following is a ‘worked example’ or web-site walkthrough of BioMart. It is probably the best way to learn how to use it! Read along, or follow on the web using the archive site for version 52 so that the layout is identical and results match up. BioMart may have been updated since the time of thi ...
Last Universal Common Ancestor
Last Universal Common Ancestor

... such as BLAST or  FASTA. These pairs can be algorithmically processed to  form clusters, representing the sets of most similar  genes across genomes. ...
Gene: Fine Structure of Gene
Gene: Fine Structure of Gene

... Transposons (Jumping Genes) Transposons or Jumping genes or Movable genes can be defined as small, mobile DNA sequences that:  move around chromosomes with no regard for homology and  insertion of these elements may produce deletions, inversions, chromosomal fusions and even more complicated rea ...
Gene Prediction in Eukaryotes
Gene Prediction in Eukaryotes

... Reliability of ORF Prediction: Characteristics of ORF regions 1. Ordered list of specific codons that reflects the evolutionary origin of the gene and constraints associated with gene expressions 2. Characteristics pattern of use of synonymous codons i.e. codons that stands for same Amino Acid 3. In ...
GEP Annotation Report - GEP Community Server
GEP Annotation Report - GEP Community Server

... The E-values for these D. suzukii matches range from 2e-10 to 1e-06 and correspond to three different predicted genes (LOC108013970, LOC108011950, and LOC108014610). All of these matches are RefSeq predictions that have not been experimentally confirmed. There are no significant matches to RefSeq re ...
GEP Annotation Report - GEP Community Server
GEP Annotation Report - GEP Community Server

... Name(s) of unique isoform(s) List of isoforms with identical coding sequences based on coding sequence CG31997-PB CG31997-PA Note: For isoforms with identical coding sequence, you only need to complete the Isoform Report Form for one of these isoforms (i.e. using the name of the isoform listed ...
Decoding the Genome of an Alien
Decoding the Genome of an Alien

... As humans, we like to think we are unique in evolutionary terms, but the octopus could reveal that this is not the case. One reason the octopus fascinates scientists is that its brain became organized to be able to carry out such incredible, complex tasks without adopting the principles of the verte ...
Measuring the Rates of Transcriptional Elongation in the Female
Measuring the Rates of Transcriptional Elongation in the Female

... germ line of any organism is unknown. Mutations in armi increase the steady-state concentration of repetitive element mRNA in the fly germ line (Vagin et al. 2006). Here, we examine the transcriptional rates of the silenced and desilenced retrotransposon gypsy and the repetitive sequence male-specif ...
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Transposable element



A transposable element (TE or transposon) is a DNA sequence that can change its position within the genome, sometimes creating or reversing mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes earned her a Nobel prize in 1983.TEs make up a large fraction of the C-value of eukaryotic cells. There are at least two classes of TEs: class I TEs generally function via reverse transcription, while class II TEs encode the protein transposase, which they require for insertion and excision, and some of these TEs also encode other proteins. It has been shown that TEs are important in genome function and evolution. In Oxytricha, which has a unique genetic system, they play a critical role in development. They are also very useful to researchers as a means to alter DNA inside a living organism.
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