Molecular evidence for the existence of additional members of the
... 3720 BA Bilthoven, The Netherlands ...
... 3720 BA Bilthoven, The Netherlands ...
Modified PDF
... is the significance of this flexibility for the function of DBP? When proline residues were introduced in the hinge region to reduce flexibility, elongation was not possible and unwinding was severely impaired (van Breukelen et al. 2000). Still DBP could bind DNA efficiently and cooperatively. This ...
... is the significance of this flexibility for the function of DBP? When proline residues were introduced in the hinge region to reduce flexibility, elongation was not possible and unwinding was severely impaired (van Breukelen et al. 2000). Still DBP could bind DNA efficiently and cooperatively. This ...
Molecular characterization of dioxygenases from polycyclic aromatic
... alignment, Lasergene software, DNASTAR). Thus, these translated polypeptides are 99.3%, 98.6% and 98.0% identical to the NidA polypeptide from M. vanbaalenii, respectively. In the case of the NidB polypeptide, the identity ...
... alignment, Lasergene software, DNASTAR). Thus, these translated polypeptides are 99.3%, 98.6% and 98.0% identical to the NidA polypeptide from M. vanbaalenii, respectively. In the case of the NidB polypeptide, the identity ...
Gene transfer in bacteria - McGraw Hill Higher Education
... Most are crucial to maintenance of earth's environment • Release oxygen to atmosphere • Recycle carbon, nitrogen, and other elements • Digest human and other animal waste • Neutralize pesticides and other pollutants • Produce vitamins and other materials essential to humans and other organisms Copyr ...
... Most are crucial to maintenance of earth's environment • Release oxygen to atmosphere • Recycle carbon, nitrogen, and other elements • Digest human and other animal waste • Neutralize pesticides and other pollutants • Produce vitamins and other materials essential to humans and other organisms Copyr ...
A systematic search for DNA methyltransferase polymorphisms
... located between PEG3 and USP29). No clear association between polymorphisms and DNA methylation values at these loci were observed. This was true for both allele-based and genotype-based association analysis (Supplementary Material, Tables S2 and 3). We then asked whether a given extended haplotype ...
... located between PEG3 and USP29). No clear association between polymorphisms and DNA methylation values at these loci were observed. This was true for both allele-based and genotype-based association analysis (Supplementary Material, Tables S2 and 3). We then asked whether a given extended haplotype ...
MICROBIAL GENETICS-III UGc - E
... circle. Such molecule will look likes figure 8. (i.e., have one cross over point or node). Instead of 360°, if it is twisted 720° prior to joining, the resulting superhelical molecule will have two nodes. The degree of twisting is about same for all molecules; namely, one negative twist is produced ...
... circle. Such molecule will look likes figure 8. (i.e., have one cross over point or node). Instead of 360°, if it is twisted 720° prior to joining, the resulting superhelical molecule will have two nodes. The degree of twisting is about same for all molecules; namely, one negative twist is produced ...
Temperate and lytic bacteriophages programmed to sensitize and
... the CRISPR array. Transcribed spacers guide Cas proteins to homologous sequences within the foreign nucleic acid, called protospacers, which are subsequently cleaved. The CRISPR-Cas systems have revolutionized molecular biology by providing efficient tools to precisely engineer genomes and manipulat ...
... the CRISPR array. Transcribed spacers guide Cas proteins to homologous sequences within the foreign nucleic acid, called protospacers, which are subsequently cleaved. The CRISPR-Cas systems have revolutionized molecular biology by providing efficient tools to precisely engineer genomes and manipulat ...
Expression of pBLU
... You have been hired to work on an extension of this Indo-Blu project. The members of your team will first learn some of the steps used to create Indo-Blu producing bacteria (using our Standard Operating Procedure). Then your team will be asked to suggest possible improvements to the bacterial transf ...
... You have been hired to work on an extension of this Indo-Blu project. The members of your team will first learn some of the steps used to create Indo-Blu producing bacteria (using our Standard Operating Procedure). Then your team will be asked to suggest possible improvements to the bacterial transf ...
Dehydrogenase in Saccharomyces cerevisiae
... Based on deletion analysis of the plasmid pGPl (Roy & Dawes, 1987), a YEp13-based vector containing a 5.5 kb yeast DNA insert carrying the LPDl gene, a 3-7 kb XhoI fragment was chosen for ‘shotgun’ cloning analysis. A region of 2701 bp has been sequenced. All of the sequence was obtained from at lea ...
... Based on deletion analysis of the plasmid pGPl (Roy & Dawes, 1987), a YEp13-based vector containing a 5.5 kb yeast DNA insert carrying the LPDl gene, a 3-7 kb XhoI fragment was chosen for ‘shotgun’ cloning analysis. A region of 2701 bp has been sequenced. All of the sequence was obtained from at lea ...
Dehydrogenase in Saccharomyces cerevisiae
... Based on deletion analysis of the plasmid pGPl (Roy & Dawes, 1987), a YEp13-based vector containing a 5.5 kb yeast DNA insert carrying the LPDl gene, a 3-7 kb XhoI fragment was chosen for ‘shotgun’ cloning analysis. A region of 2701 bp has been sequenced. All of the sequence was obtained from at lea ...
... Based on deletion analysis of the plasmid pGPl (Roy & Dawes, 1987), a YEp13-based vector containing a 5.5 kb yeast DNA insert carrying the LPDl gene, a 3-7 kb XhoI fragment was chosen for ‘shotgun’ cloning analysis. A region of 2701 bp has been sequenced. All of the sequence was obtained from at lea ...
Sequence, expression, and characterization of the first archaeal ATP
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
... and eukaryal species. A general feature of most ATPPFKs from the domains of Bacteria and Eukarya is their homotetrameric stucture and the allosteric regulation of activity by compounds of intermediary metabolism. The bacterial enzymes are usually composed of 34-kDa subunits, allosterically activated ...
The Differential Killing of Genes by Inversions in Prokaryotic Genomes
... determines the described location of a gene. If the sense strand is located on the leading strand, it is assumed that “the gene lies on the leading strand.” In prokaryotic genomes, the leading and lagging roles of DNA strands are predetermined by location of the origin of replication and the terminu ...
... determines the described location of a gene. If the sense strand is located on the leading strand, it is assumed that “the gene lies on the leading strand.” In prokaryotic genomes, the leading and lagging roles of DNA strands are predetermined by location of the origin of replication and the terminu ...
Chapter 2: Introduction to Molecular Genetics
... Tertiary structure of DNA This structure refers to how DNA is stored in a confined space to form the chromosomes. It varies depending on whether the organisms prokaryotes and eukaryotes: - In prokaryotes the DNA is folded like a super-helix, usually in circular shape and associated with a small ...
... Tertiary structure of DNA This structure refers to how DNA is stored in a confined space to form the chromosomes. It varies depending on whether the organisms prokaryotes and eukaryotes: - In prokaryotes the DNA is folded like a super-helix, usually in circular shape and associated with a small ...
Virus induced gene silencing, a post transcriptional gene silencing
... one of these tools to suppress expression level of the gene of interest in plants [3; 4]. The term VIGS was first coined by A. van Kammen to describe the resistance event against viral infection [5]. Since plants infected by many viruses induce RNA mediated defense which targets viral RNAs and any t ...
... one of these tools to suppress expression level of the gene of interest in plants [3; 4]. The term VIGS was first coined by A. van Kammen to describe the resistance event against viral infection [5]. Since plants infected by many viruses induce RNA mediated defense which targets viral RNAs and any t ...
The DnaE polymerase from Deinococcus radiodurans features
... interacts with the core α subunit. The β-clamp is a homodimer, and confers to the holoenzyme high processivity by tethering the polymerase to the DNA substrate [20, 21]. In addition, the progression of a replication fork is assisted by the association of DNA Pol III with DNA helicase and primase [22 ...
... interacts with the core α subunit. The β-clamp is a homodimer, and confers to the holoenzyme high processivity by tethering the polymerase to the DNA substrate [20, 21]. In addition, the progression of a replication fork is assisted by the association of DNA Pol III with DNA helicase and primase [22 ...
Nucleotide sequence and structural organization of
... The study of small plasmids from a variety of bacteria has recently become a focus of research interest and in several cases this work has led to the development of families of vectors designed for specific purposes. The genetic organization and mode of replication of a number of small cryptic plasm ...
... The study of small plasmids from a variety of bacteria has recently become a focus of research interest and in several cases this work has led to the development of families of vectors designed for specific purposes. The genetic organization and mode of replication of a number of small cryptic plasm ...
Target selected insertional mutagenesis on chromosome IV of
... Jones et al., 1994; Okuley et al., 1994; AzpirozLeehan and Feldmann, 1997). Insertional mutagenesis can also be used to identify insertions in specific target genes with known sequence but unknown function. This reverse genetics procedure termed target selected insertional mutagenesis uses T-DNA or ...
... Jones et al., 1994; Okuley et al., 1994; AzpirozLeehan and Feldmann, 1997). Insertional mutagenesis can also be used to identify insertions in specific target genes with known sequence but unknown function. This reverse genetics procedure termed target selected insertional mutagenesis uses T-DNA or ...
BIO450 Primer Design Tutorial
... primer design for target specificity. Of course, you might want to amplify a family of genes, in which case you would aim for a different level of specificity. Primers are short single-stranded oligonucleotides (‘primers’) which hybridize (‘anneal’) to one strand of the target DNA (the ‘template’). ...
... primer design for target specificity. Of course, you might want to amplify a family of genes, in which case you would aim for a different level of specificity. Primers are short single-stranded oligonucleotides (‘primers’) which hybridize (‘anneal’) to one strand of the target DNA (the ‘template’). ...
Activity Name - Science4Inquiry.com
... - Newly synthesized DNA model - DNA replication cards 1. After completing your newly synthesized DNA through DNA replication, obtain the DNA replication cards from your teacher and do the following: a. Match the DNA Replication Card to your DNA sequence to find out where your model of DNA replicatio ...
... - Newly synthesized DNA model - DNA replication cards 1. After completing your newly synthesized DNA through DNA replication, obtain the DNA replication cards from your teacher and do the following: a. Match the DNA Replication Card to your DNA sequence to find out where your model of DNA replicatio ...
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... enrichment at sub-telomeric regions was observed in growing yeast cells (15,22). The sub-telomeric enrichment of γ-H2A depended on the yeast orthologue of ATM and was distinct from internal γ-H2A formation, which depended mostly on the orthologue of ATR (22). It had been thought that telomeres shoul ...
... enrichment at sub-telomeric regions was observed in growing yeast cells (15,22). The sub-telomeric enrichment of γ-H2A depended on the yeast orthologue of ATM and was distinct from internal γ-H2A formation, which depended mostly on the orthologue of ATR (22). It had been thought that telomeres shoul ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.