Conjugative plasmids: vessels of the communal gene pool
... transposases, which catalyse the movement and insertion (sometimes replicative) of transposons, integrases, which enable insertion of elements such as gene cassettes into integrons by site-specific recombination, and resolvases, which are DNA endonucleases capable of resolving Holiday junctions that ...
... transposases, which catalyse the movement and insertion (sometimes replicative) of transposons, integrases, which enable insertion of elements such as gene cassettes into integrons by site-specific recombination, and resolvases, which are DNA endonucleases capable of resolving Holiday junctions that ...
SEGREGATION RATIOS–general reference
... • Assumes no recombination and is therefore only relevant to the centromere and loci closely linked to it. (100% reductional separations at Anaphase I). 2. Random chromatid segregation (Haldane, 1930) • Each chromosome could have multiple pairing partners at any time at the most extreme, a chromosom ...
... • Assumes no recombination and is therefore only relevant to the centromere and loci closely linked to it. (100% reductional separations at Anaphase I). 2. Random chromatid segregation (Haldane, 1930) • Each chromosome could have multiple pairing partners at any time at the most extreme, a chromosom ...
DNA Duplication Associated with Charcot-Marie-Tooth Disease Type 1A. Lupski, et al., 1991 Cell, Vol. 66, 219-232, July 26, 1991,
... third type is unlinked to both the CMTlA and CMTIB loci (Chance et al., 1990). These studies provide the basis for isolating the disease gene(s) by virtue of map position. Positional cloning experiments can be aided by the existence of patients with specific chromosomal DNA rearrangements. However, ...
... third type is unlinked to both the CMTlA and CMTIB loci (Chance et al., 1990). These studies provide the basis for isolating the disease gene(s) by virtue of map position. Positional cloning experiments can be aided by the existence of patients with specific chromosomal DNA rearrangements. However, ...
CfE Higher Biology Unit 1: DNA and the Genome
... demonstrated two findings, now known as Chargaff’s rules: firstly, that adenine and thymine always occur together, and similarly that cytosine and guanine pair up - this is called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalin ...
... demonstrated two findings, now known as Chargaff’s rules: firstly, that adenine and thymine always occur together, and similarly that cytosine and guanine pair up - this is called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalin ...
Nucleic Acids
... 20.1 What Are Nucleosides and Nucleotides? Controlled hydrolysis of nucleic acids yields three types of simpler building blocks: heterocyclic aromatic amine bases, the monosaccharide d-ribose or 2-deoxy-d-ribose (Section 17.3), and phosphate ions. Figure 20.1 shows the five heterocyclic aromatic ami ...
... 20.1 What Are Nucleosides and Nucleotides? Controlled hydrolysis of nucleic acids yields three types of simpler building blocks: heterocyclic aromatic amine bases, the monosaccharide d-ribose or 2-deoxy-d-ribose (Section 17.3), and phosphate ions. Figure 20.1 shows the five heterocyclic aromatic ami ...
D-loop - BioMed Central
... aminoacid composition in this mite species as reported previously for flatworms [3], and may be source of significant artifacts in phylogenetic interference. There is a clear preference for GT-rich codons on the majority strand, while AC-rich codons were more frequent on the minority strands, howeve ...
... aminoacid composition in this mite species as reported previously for flatworms [3], and may be source of significant artifacts in phylogenetic interference. There is a clear preference for GT-rich codons on the majority strand, while AC-rich codons were more frequent on the minority strands, howeve ...
The first page should show the paper title, names and addresses of
... Galliformes could be resolved using the FISH on lampbrush chromosomes as an investigative procedure? On the other hand, why should we still use time-consuming methods like comparative FISH mapping on LBCs when next-generation sequencing approaches are opening up new opportunities for genome-wide ana ...
... Galliformes could be resolved using the FISH on lampbrush chromosomes as an investigative procedure? On the other hand, why should we still use time-consuming methods like comparative FISH mapping on LBCs when next-generation sequencing approaches are opening up new opportunities for genome-wide ana ...
Roles of the Amino Group of Purine Bases in the Thermodynamic
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
... important for the creation of secondary and higher-order structures and the practical application of oligonucleotides for hybridization-based detection and targeting assays. However, the small differences between the interaction energies of complementary and mismatched pairs cause a difficulty in di ...
Antisense Transcript and RNA Processing
... (A) RNA gel blot analysis. Total RNA (5 mg) from strains shown across the top were probed, as shown at the left of each panel. The portion of the trnE1 blot showing the native tRNAGlu species (75 nucleotides) has been cropped. Sizes of transcripts, shown at right, were calibrated from RNA size stand ...
... (A) RNA gel blot analysis. Total RNA (5 mg) from strains shown across the top were probed, as shown at the left of each panel. The portion of the trnE1 blot showing the native tRNAGlu species (75 nucleotides) has been cropped. Sizes of transcripts, shown at right, were calibrated from RNA size stand ...
64$ CfE Higher Biology Unit 1: DNA and the
... demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and thymine always occur together, and similarly that cytosine and guanine pair up - this is called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalin ...
... demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and thymine always occur together, and similarly that cytosine and guanine pair up - this is called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalin ...
64$ CfE Higher Biology Unit 1: DNA and the
... demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and thymine always occur together, and similarly that cytosine and guanine pair up - this is called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalin ...
... demonstrated two findings, now known as Chargaff's rules: firstly, that adenine and thymine always occur together, and similarly that cytosine and guanine pair up - this is called base pairing; secondly, that DNA sequences vary between species. In the early 1950s, work by Maurice Wilkins and Rosalin ...
Properties and sequence of the coenzyme B12
... HindIII, and ligated into the vector pBluescript SK+, which had been linearized with the corresponding enzymes. Ligated DNA was transformed in E. coli JM109. The genes coding for glycerol dehydratase of C. pasteurianum were located on 3 recombinant E. coli strains with di¡erent inserts, one containi ...
... HindIII, and ligated into the vector pBluescript SK+, which had been linearized with the corresponding enzymes. Ligated DNA was transformed in E. coli JM109. The genes coding for glycerol dehydratase of C. pasteurianum were located on 3 recombinant E. coli strains with di¡erent inserts, one containi ...
The Mammalian Mismatch Repair Pathway Removes DNA 8
... 3A). Surprisingly, despite their high levels of DNA 8-oxoG, Colo26 cell extracts contained significantly more Ogg1 activity than extracts of MEFs. Colo26, Colo5, and Colo5 ⫹ ch 2 extracts all incised the 8-oxoG duplex approximately 10-fold more efficiently than the MEF extracts (Figure 3B). There we ...
... 3A). Surprisingly, despite their high levels of DNA 8-oxoG, Colo26 cell extracts contained significantly more Ogg1 activity than extracts of MEFs. Colo26, Colo5, and Colo5 ⫹ ch 2 extracts all incised the 8-oxoG duplex approximately 10-fold more efficiently than the MEF extracts (Figure 3B). There we ...
Molecular Microbial Ecology of the Rhizosphere
... horizontal gene transfer (Chen et al., 2007). The approximately 150 genes only occurring in the plant-associated subspecies “plantarum” were found mainly clustered in the GIs. Generally, such genes are termed singletons, meaning that no orthologous genes can be identified in any other strain of the ...
... horizontal gene transfer (Chen et al., 2007). The approximately 150 genes only occurring in the plant-associated subspecies “plantarum” were found mainly clustered in the GIs. Generally, such genes are termed singletons, meaning that no orthologous genes can be identified in any other strain of the ...
Rate of Gene Transfer From Mitochondria to Nucleus
... mitochondria lacking sufficient genetic information. There is no direct evidence for the intracellular selection for the rapid replication of mitochondria, although it has been suggested by the dynamics of yeast mitochondria involving good markers (respiration-deficient mutants, or petites). When he ...
... mitochondria lacking sufficient genetic information. There is no direct evidence for the intracellular selection for the rapid replication of mitochondria, although it has been suggested by the dynamics of yeast mitochondria involving good markers (respiration-deficient mutants, or petites). When he ...
Applied Environmnetal Microbiology
... RESULTS Screening. We prepared the cryV-specific probe from total DNA of B. thuringiensis subsp. kurstaki HD-1 by PCR as described above. Nucleotide sequences of the 0.7-kb PCR product in both strands were analyzed (data not shown), and it was confirmed that sequences in the probe region of the cryV ...
... RESULTS Screening. We prepared the cryV-specific probe from total DNA of B. thuringiensis subsp. kurstaki HD-1 by PCR as described above. Nucleotide sequences of the 0.7-kb PCR product in both strands were analyzed (data not shown), and it was confirmed that sequences in the probe region of the cryV ...
Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of
... Earlier, Gray and Doolittle (1 1) argued that the strongest evidence for the proposed prokaryotic nature of mtDNA was derived from T1 oligonucleotide comparisons of wheat mitochondrial 18S rRNA with eubacterial 16S and other small subunit rRNAs. More recently, Schnare and Gray (20) have sequenced th ...
... Earlier, Gray and Doolittle (1 1) argued that the strongest evidence for the proposed prokaryotic nature of mtDNA was derived from T1 oligonucleotide comparisons of wheat mitochondrial 18S rRNA with eubacterial 16S and other small subunit rRNAs. More recently, Schnare and Gray (20) have sequenced th ...
7) NATURAL SELECTION: the process by which forms of life having
... almost identical pairs. • Chromosomes have specific active locations called alleles. • The two alleles in identical locations on paired chromosomes constitute a gene ...
... almost identical pairs. • Chromosomes have specific active locations called alleles. • The two alleles in identical locations on paired chromosomes constitute a gene ...
Considerations for Analyzing Targeted NGS Data – HLA
... Lots of similar genes and lots of very similar pseudegenes. Duplicated segments can be more similar to each other within an individual than they are similar to the corresponding segments of the reference genome. ...
... Lots of similar genes and lots of very similar pseudegenes. Duplicated segments can be more similar to each other within an individual than they are similar to the corresponding segments of the reference genome. ...
Aucun titre de diapositive - Universidad Nacional De Colombia
... out vector sequences (vector clipping). out repeat sequences (repeats masking). out low complexity sequences. ...
... out vector sequences (vector clipping). out repeat sequences (repeats masking). out low complexity sequences. ...
PDF - WashU Epigenome Browser
... Learn more about the supported numerical track formats bedGraph (http: //wiki.wubrowse.org/bedgraph) and bigWig (http://wiki.wubrowse.org/bigwig). ...
... Learn more about the supported numerical track formats bedGraph (http: //wiki.wubrowse.org/bedgraph) and bigWig (http://wiki.wubrowse.org/bigwig). ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.