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Microsoft Word - Mapping-Traits-in-Dogs
Microsoft Word - Mapping-Traits-in-Dogs

... Ostrander, Ph.D., chief of the Cancer Genetics Branch in NHGRI's Division of Intramural Research. "We think this approach will help pinpoint multiple genes involved in complex human conditions, such as cancer, heart disease, diabetes and obesity." Artificial selection, at the heart of breeding for d ...
(a) (b)
(a) (b)

... © 2011 Pearson Education, Inc. ...
Rich Probabilistic Models for Genomic Data
Rich Probabilistic Models for Genomic Data

... Recombinant haplotype ...
Temperature-sensitive control of protein activity by conditionally
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DNA methylation involved in proline accumulation in - Funpec-RP
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... 1% agarose gels and transferred to Hybond N+ nylon membranes (Amersham Pharmacia Biotech, Piscataway, NJ, USA) using the alkaline transfer recommended by the supplier. These probes were prepared via sequence-specific polymerase chain reaction (PCR) amplifications using specific primers (Table S2). T ...
Characterization of sex chromosomes in rainbow trout and coho
Characterization of sex chromosomes in rainbow trout and coho

... This chromosome comparison suggests conserved uniarmed morphology of sex chromosomes among these species. Given the evidence of the localization of the rainbow trout sex marker OmyP9, we suggest that this subtelocentric pair could be the sex chromosomes in coho salmon based as well as the morphologi ...
Identification of genes that are associated with DNA repeats in
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... prokaryotic organisms, and their identification is increasingly facilitated by the availability of sequences of complete bacterial genomes. The length, sequence and position of these sequences in the genome are highly variable and often unique for a single strain (Lupski et al., 1996; Bachellier et ...
Problem Set V - Biology 2970
Problem Set V - Biology 2970

... In general, individuals with Down's syndrome are trisomic for a small acrocentric chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in ...
MacVector 14.0 Getting Started Guide
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... The cDNA Alignment algorithm lets you align one or more cDNA clones against a reference genomic sequence. This can be used for identifying where exon/intron boundaries lie in your cDNA/mRNA sequences. It differs ...
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... Some important points to remember about chromosomes. 1. Chromosomes were first observed by Straburger (1875) in mitotically dividing cells and the name ‘chromosome’ was proposed by Waldeyer in 1888. 2. Each species has a definite and constant number of chromosomes in their cells. The chromosome num ...
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... unable to survive blasticidin-S treatment. The silenced episome assumes position 2 (transcriptionally active region), which allows the hsp86 promoter to transcribe the bsd resistance gene (black). However, the upsC promoter driving expression of hdhfr remains silenced due to the interaction of silen ...
Antimicrobial Agents and Chemotherapy
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... of reactions has been found in gram-negative bacteria (1, 11). Until recently, resistance of gram-positive bacteria to aminoglycosides has scarcely been examined; however, inactivation of kanamycin by a resistant strain of Staphylococcus aureus has been reported (5). This phenomenon was later demons ...
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... requires large amounts of genomic DNA. In addition, restriction site loss during ...
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... concentration along the genome (David et al, 2006). Allele expression levels and probe affinities in our non-linear, heteroscedastic model were inferred using iterative weighted least squares (see Materials and methods). Confidence intervals were obtained by bootstrap re-sampling of the residuals. A ...
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Complete genome sequence of the thermophilic Thermus sp

... are easier to crystallize than their mesophilic counterparts [7]. Some of the breakthrough examples of large complexes from thermophiles that have been crystallized are structures of the 70S ribosome [8], the bacterial RNA polymerase [9, 10] and the respiratory complex I [11] from Thermus spp. that ...
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The landscape of microbial phenotypic traits and associated genes

... not be represented in existing databases. To this end, we constructed a standard ‘bag-of-words’ representation: a matrix where rows correspond to words and columns to organisms for each of the five corpora separately (excluding the mixed collection). In order to enforce consistency between corpora, ...
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... Fragile sites are regions within the genome prone to breaking when a cell is undergoing stressed replication (Le Tallec et al., 2014). The study of fragile sites began with the study of the FRAXA rare fragile site, and this field of investigation grew when breaks at other regions in the human genom ...
The influence of genomic imprinting on brain
The influence of genomic imprinting on brain

... provide most of the postfertilization nutrition. In these conditions, the probability that the identical paternally derived allele will be found in multiple offspring of the same female is low ( < 0.5), compared to the probability for a maternally derived allele ( = 0.5) (Hurst & McVean, 1997). Thus ...
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Adaptation of Sucrose Metabolism in the Escherichia coli Wild

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... phase, and they remain as tight foci until completion of replication (metaphase), and they seem to disassemble. Second, mutations that delay DNA synthesis (deletion of the mitotic cyclin genes CLB5 and CLB6) without affecting bud emergence also delay the formation of the fluorescent foci. Third, whe ...
20 years and 22 papers with Bernard Moret
20 years and 22 papers with Bernard Moret

... Phylogeny reconstruction in 1999 • Distance-based – Breakpoint (BP) distances [Blanchette, Kunisawa, Sankoff 1999] – fast but high error • Breakpoint tree (NP-hard, even for three taxa) – BPAnalysis: [Sankoff & Blanchette 1998]: exhaustive search through treespace to find the minimum breakpoint len ...
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...  Expose Chado data to Drupal Panels in the form of blocks  Allows tripal administrators to arrange chado content on details pages  Decide if you want the Sequence Features page to only contain basic details and other details ...
Aberrant replication timing induces defective chromosome
Aberrant replication timing induces defective chromosome

... © 2000 Elsevier Science Ltd. All rights reserved. ...
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... The   code   in   the   class  DNABenchMark   can   be   used   to   benchmark   the  cutAndSplice  method.   The   code   given   to   you   will   pop-­‐up   a   file-­‐dialog   box   —   when   run   you   can   use   this   to   nav ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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