Recombinant Materials Form
... transferred to another host by well-established physiological means? 14. Will the experiment include recombinant or synthetic nucleic acids that consist entirely of nucleic acids from a eukaryotic host including its chloroplasts mitochondria, or plasmids (but excluding viruses) when propagated only ...
... transferred to another host by well-established physiological means? 14. Will the experiment include recombinant or synthetic nucleic acids that consist entirely of nucleic acids from a eukaryotic host including its chloroplasts mitochondria, or plasmids (but excluding viruses) when propagated only ...
- Wiley Online Library
... products were similar to the active a-ketoglutarate dehydrogenase subunits of heterotrophs. These obligate species are thus genomically competent with respect to this enzyme but are apparently incapable of producing a functional enzyme. Probing of the full and incomplete genomes of some cyanobacteri ...
... products were similar to the active a-ketoglutarate dehydrogenase subunits of heterotrophs. These obligate species are thus genomically competent with respect to this enzyme but are apparently incapable of producing a functional enzyme. Probing of the full and incomplete genomes of some cyanobacteri ...
The importance of alternative splicing in the drug discovery process
... This approach was also employed to alter the alternative was not knocked out [29]. Thus, when trying to shut down splicing of the Tau gene that causes frontotemporal dethe activity of a gene, the knockout cassette should be inmentia and parkinsonism linked to chromosome 17 [26]. serted into an exon ...
... This approach was also employed to alter the alternative was not knocked out [29]. Thus, when trying to shut down splicing of the Tau gene that causes frontotemporal dethe activity of a gene, the knockout cassette should be inmentia and parkinsonism linked to chromosome 17 [26]. serted into an exon ...
DNA Polyacrylamide Gel Electrophoresis
... 7. After polymerization is complete, surround the comb and the top of the gel with paper towels that have been soaked in 1x TBE. Then seal the entire gel in Saran Wrap or plastic bag and store it at 4°C until needed. 8. When ready to proceed with electrophoresis, remove gels from gel caster, careful ...
... 7. After polymerization is complete, surround the comb and the top of the gel with paper towels that have been soaked in 1x TBE. Then seal the entire gel in Saran Wrap or plastic bag and store it at 4°C until needed. 8. When ready to proceed with electrophoresis, remove gels from gel caster, careful ...
A DNA Polymerase ε Mutant That Specifically Causes 1
... 1997b). The absence of RAD27 can result in increased mutation rates including expansion of repeat sequences and large duplications (Tishkoff et al. 1997b; Freudenreich et al. 1998; Kokoska et al. 1998; Maurer et al. 1998). Furthermore, a structure with mispaired nucleotides in close association with ...
... 1997b). The absence of RAD27 can result in increased mutation rates including expansion of repeat sequences and large duplications (Tishkoff et al. 1997b; Freudenreich et al. 1998; Kokoska et al. 1998; Maurer et al. 1998). Furthermore, a structure with mispaired nucleotides in close association with ...
Genetic identification of eleven aquatic bacteria using the 16S rDNA
... The genetic methods include the molecular analysis of DNA where DNA sections like genes or the entire genome are used to identify bacteria. Genetic identification using the entire genome is more accurate than using just one gene, like the 16S rDNA, however it is more time-consuming and expensive. Th ...
... The genetic methods include the molecular analysis of DNA where DNA sections like genes or the entire genome are used to identify bacteria. Genetic identification using the entire genome is more accurate than using just one gene, like the 16S rDNA, however it is more time-consuming and expensive. Th ...
Gill: Gene Regulation II
... • An antibody is a large Y-shaped protein used by the immune system to identify and neutralize foreign objects such as bacteria. • Antibodies can be raised that instead recognize specific transcription factors. • Chromatin Immunoprecipitation followed by deep sequencing (ChIP-seq): Take DNA (region ...
... • An antibody is a large Y-shaped protein used by the immune system to identify and neutralize foreign objects such as bacteria. • Antibodies can be raised that instead recognize specific transcription factors. • Chromatin Immunoprecipitation followed by deep sequencing (ChIP-seq): Take DNA (region ...
Exam 2
... 5. The F-plasmid can be stabily maintained in both Salmonella typhimurium and Escherichia coli. However, Hfr’s are formed much less frequently in S. typhimurium than in E. coli. In addition, there are many fewer insertion sites in S. typhimurium compared to E. coli. Given what you know about how Hfr ...
... 5. The F-plasmid can be stabily maintained in both Salmonella typhimurium and Escherichia coli. However, Hfr’s are formed much less frequently in S. typhimurium than in E. coli. In addition, there are many fewer insertion sites in S. typhimurium compared to E. coli. Given what you know about how Hfr ...
toxicity in bread wheat - BMC Plant Biology
... Aluminium (Al) toxicity is one of the major concerns for crop productivity in acidic soil, accounting for more than 50% of the global arable land [1]. Al is one of the highly abundant elements in the earth crust and under low pH conditions (acidic soils), it is solubilised in the soil in a toxic ion ...
... Aluminium (Al) toxicity is one of the major concerns for crop productivity in acidic soil, accounting for more than 50% of the global arable land [1]. Al is one of the highly abundant elements in the earth crust and under low pH conditions (acidic soils), it is solubilised in the soil in a toxic ion ...
Complementary DNA Sequence of a Human Cytoplasmic Actin
... been inserted into the Hind111 site of pBR322, and was excised intact by treatment with this enzyme. This cDNA insert was labeled with (n-32P]dCTP using oligomeric calf thymus DNA fragments as random primers, and reverse transcriptase as a DN,Mependent Dl\‘A polymerase (Fuchs et ~1.. 1981). When the ...
... been inserted into the Hind111 site of pBR322, and was excised intact by treatment with this enzyme. This cDNA insert was labeled with (n-32P]dCTP using oligomeric calf thymus DNA fragments as random primers, and reverse transcriptase as a DN,Mependent Dl\‘A polymerase (Fuchs et ~1.. 1981). When the ...
The E1 ubiquitin-activating enzyme Uba1 in Drosophila controls
... judged by the absence of twin-spots) and this half was normal in size, thus serving as an internal control (see right half of the head in Fig. 2G), whereas the other side of the head contained twinspots and was overgrown (left half in Fig. 2G). Third instar larval eye-antennal imaginal discs of Uba1 ...
... judged by the absence of twin-spots) and this half was normal in size, thus serving as an internal control (see right half of the head in Fig. 2G), whereas the other side of the head contained twinspots and was overgrown (left half in Fig. 2G). Third instar larval eye-antennal imaginal discs of Uba1 ...
abstracts
... all tested major and minor root-knot nematode (RKN) species including a Florida population that overcomes the resistance of other commonly used Prunus resistance sources from the Amygdalus subgenus. The RKN resistant Myrobalan plum clone P.2175 is heterozygous for Ma. The gene was mapped on linkage ...
... all tested major and minor root-knot nematode (RKN) species including a Florida population that overcomes the resistance of other commonly used Prunus resistance sources from the Amygdalus subgenus. The RKN resistant Myrobalan plum clone P.2175 is heterozygous for Ma. The gene was mapped on linkage ...
Lesson Overview - Dr. Thornton`s Courses
... mutants—individuals with mutations—with useful characteristics that are not found in the original population. For example, scientists have developed hundreds of useful mutant bacterial strains by treating bacteria with radiation or chemicals. Certain strains of oil-digesting bacteria are effective f ...
... mutants—individuals with mutations—with useful characteristics that are not found in the original population. For example, scientists have developed hundreds of useful mutant bacterial strains by treating bacteria with radiation or chemicals. Certain strains of oil-digesting bacteria are effective f ...
Information Encoding in Biological Molecules: DNA and
... • The microRNA bantam regulates the Drosophila (fruitfly) gene hid by binding the 3’ UTR. Hid is involved in apoptosis, and it is possible that binding sites for bantam could be found in the 3’ UTR of other apoptosis genes as well. Obtain the 3’ UTR sequence of all Drosophila genes known to be invol ...
... • The microRNA bantam regulates the Drosophila (fruitfly) gene hid by binding the 3’ UTR. Hid is involved in apoptosis, and it is possible that binding sites for bantam could be found in the 3’ UTR of other apoptosis genes as well. Obtain the 3’ UTR sequence of all Drosophila genes known to be invol ...
Lab 7: Conjugation/Transformation
... E. coli. Transformation is the uptake of naked DNA by bacteria; many bacteria are able to take up DNA readily from their environment, although E. coli must first be treated with calcium chloride or other salts. Transduction is the transfer of genes mediated by viruses. This lab will introduce the tr ...
... E. coli. Transformation is the uptake of naked DNA by bacteria; many bacteria are able to take up DNA readily from their environment, although E. coli must first be treated with calcium chloride or other salts. Transduction is the transfer of genes mediated by viruses. This lab will introduce the tr ...
Chapter 13 Meiosis
... sister chromatid cohesion. The sister chromatids make one duplicate chromosome; this is different from homologous chromosomes, which are inherited from different parents. Homologs may have different versions of a gene each called an allele. The phases of meiosis are similar to those of mitosis but w ...
... sister chromatid cohesion. The sister chromatids make one duplicate chromosome; this is different from homologous chromosomes, which are inherited from different parents. Homologs may have different versions of a gene each called an allele. The phases of meiosis are similar to those of mitosis but w ...
Topologically Non-linked Circular Duplex DNA
... Left-handed DNA has been known for many years (Mitsui et al., 1970; Ikehara et al., 1972; Pohl and Jovin, 1972; Pohl, 1976; Wang et al., 1979; Nordheim et al., 1981). It was first observed in synthetic copolymers. It is not considered to be the structure of purified DNA in solution for any DNA obtai ...
... Left-handed DNA has been known for many years (Mitsui et al., 1970; Ikehara et al., 1972; Pohl and Jovin, 1972; Pohl, 1976; Wang et al., 1979; Nordheim et al., 1981). It was first observed in synthetic copolymers. It is not considered to be the structure of purified DNA in solution for any DNA obtai ...
Phylogenetic and evolutionary analyses of St. Louis encephalitis
... Phylogenetic relationships were evaluated using maximum likelihood (ML) and Bayesian methods. ML trees were generated with PHYML v2.4.4 (Guindon and Gascuel, 2003), following testing for appropriate models using Modeltest (Posada and Crandall, 1998) and PAUP* v4b10 (Swofford, 2003), using the GTR sub ...
... Phylogenetic relationships were evaluated using maximum likelihood (ML) and Bayesian methods. ML trees were generated with PHYML v2.4.4 (Guindon and Gascuel, 2003), following testing for appropriate models using Modeltest (Posada and Crandall, 1998) and PAUP* v4b10 (Swofford, 2003), using the GTR sub ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.