
paramecium tetraurelia
... Crosses between cells of diferent mating types. The two mating types, 0 and E, have previously been named VI1 and VIII. Genetic analysis was carried out according to the methods developed and reviewed by SONNEBORN (1970). The two main features of the analysis are: (1) i n a cross A x B the two excon ...
... Crosses between cells of diferent mating types. The two mating types, 0 and E, have previously been named VI1 and VIII. Genetic analysis was carried out according to the methods developed and reviewed by SONNEBORN (1970). The two main features of the analysis are: (1) i n a cross A x B the two excon ...
Slide - Gerstein Lab
... • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with ...
... • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with ...
Integrated Microbial Genomes
... supplement to the COGs is available, in which proteins encoded in the genomes of two multicellular eukaryotes, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, and shared with bacteria and/or archaea were included. The new features added to the COG database include info ...
... supplement to the COGs is available, in which proteins encoded in the genomes of two multicellular eukaryotes, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, and shared with bacteria and/or archaea were included. The new features added to the COG database include info ...
Journal of Bacteriology
... features described below, is illustrated in Fig. 2. A screening for sequence homology with the consensus sequence of the nod box (a general feature of flavonoid-inducible nod genes [25]) revealed a nod box-like sequence (Fig. 2) located within a PstI-BamHI fragment. A long open reading frame startin ...
... features described below, is illustrated in Fig. 2. A screening for sequence homology with the consensus sequence of the nod box (a general feature of flavonoid-inducible nod genes [25]) revealed a nod box-like sequence (Fig. 2) located within a PstI-BamHI fragment. A long open reading frame startin ...
genetics - Gene In The Title
... http://www.nytimes.com/2010/08/14/business/14sugar.htm l?src=sch&pagewanted=all • Disease Cause Is Pinpointed With Genome mar 10, 10 ...
... http://www.nytimes.com/2010/08/14/business/14sugar.htm l?src=sch&pagewanted=all • Disease Cause Is Pinpointed With Genome mar 10, 10 ...
Transgenic Plastids in Basic Research and Plant Biotechnology
... compartment (for reviews see, e.g. GoldschmidtClemont,3 Leon et al.4 and Coleman and Nerozzi5). More recently, chloroplast research has bene®ted enormously from the introduction of transgenic technologies facilitated by the development of reliable methods for plastid genome transformation.6-8 This m ...
... compartment (for reviews see, e.g. GoldschmidtClemont,3 Leon et al.4 and Coleman and Nerozzi5). More recently, chloroplast research has bene®ted enormously from the introduction of transgenic technologies facilitated by the development of reliable methods for plastid genome transformation.6-8 This m ...
Comparative analysis of peanut NBS‐LRR gene clusters suggests
... was also found to have three RGAs (Mt7g088460.1, Mt7g088470.1, Mt7g099490.1) that shared high homology to the peanut RGAs. These two regions (BAC AC169666 and AC149204) are separated by c. 250 kb on Medicago chromosome 7. This finding indicates that disease resistance genes were present in this regi ...
... was also found to have three RGAs (Mt7g088460.1, Mt7g088470.1, Mt7g099490.1) that shared high homology to the peanut RGAs. These two regions (BAC AC169666 and AC149204) are separated by c. 250 kb on Medicago chromosome 7. This finding indicates that disease resistance genes were present in this regi ...
Questions - nslc.wustl.edu
... In general, individuals with Down's syndrome are trisomic for a small acrocentric chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in ...
... In general, individuals with Down's syndrome are trisomic for a small acrocentric chromosome that is designated chromosome 21. Such trisomic individuals have 47 chromosomes rather than the normal 46. Down's syndrome patients that have 46 chromosomes are occasionally found, however. Almost always in ...
Honors Biology Portfolio
... Bio 3.4 Explain the theory of evolution by natural selection as a mechanism for how species change over time. Bio 3.4.1 Explain how fossil, biochemical, and anatomical evidence support the theory of evolution (honors extension to include the historical perspective on the theory of evolution beginnin ...
... Bio 3.4 Explain the theory of evolution by natural selection as a mechanism for how species change over time. Bio 3.4.1 Explain how fossil, biochemical, and anatomical evidence support the theory of evolution (honors extension to include the historical perspective on the theory of evolution beginnin ...
Chromosomal Microarray Analysis
... copy number in the distal and subtelomeric regions of the long arm of chromosome 18 suggestive of mosaicism. This deletion includes the critical region of chromosome 18q deletion syndrome (OMIM 601808). FISH analysis and partial chromosome analysis revealed mosaicism for a deletion in the long arm o ...
... copy number in the distal and subtelomeric regions of the long arm of chromosome 18 suggestive of mosaicism. This deletion includes the critical region of chromosome 18q deletion syndrome (OMIM 601808). FISH analysis and partial chromosome analysis revealed mosaicism for a deletion in the long arm o ...
Clinical use of Whole Genome Sequencing for Mycobacterium
... problem, however it is a more straightforward task for M. tuberculosis than for other species due to a lack of horizontal gene transfer; indeed, drug resistance is generally thought to be mediated only through mutations in specific gene targets. The key therefore is to identify those single nucleoti ...
... problem, however it is a more straightforward task for M. tuberculosis than for other species due to a lack of horizontal gene transfer; indeed, drug resistance is generally thought to be mediated only through mutations in specific gene targets. The key therefore is to identify those single nucleoti ...
Identification and removal of colanic acid from plasmid DNA
... remove CA from plasmid DNA preparations. Furthermore, our data suggest that because CA is mainly longchained, branched, and has high molecular weight (MW), this polysaccharide is most refractory to animals. CA is found in high abundance and comprises approximately 25% of the bacterial cell wall of g ...
... remove CA from plasmid DNA preparations. Furthermore, our data suggest that because CA is mainly longchained, branched, and has high molecular weight (MW), this polysaccharide is most refractory to animals. CA is found in high abundance and comprises approximately 25% of the bacterial cell wall of g ...
Sequence of the Tribolium castaneum Homeotic Complex
... In the following descriptions of Tribolium homeobox genes, the total length of the transcription units and the length of the large intron upstream of the homeobox (when present) were determined from the sequence described here (Table 1). Exon sizes reported here confirm previous analyses and are inc ...
... In the following descriptions of Tribolium homeobox genes, the total length of the transcription units and the length of the large intron upstream of the homeobox (when present) were determined from the sequence described here (Table 1). Exon sizes reported here confirm previous analyses and are inc ...
Whole-transcriptome RNAseq analysis from minute amount of total
... RNA-seq methods, some of which are described in literature (10): library complexity, the number of unique reads, ribosomal RNA read-count in comparison to total reads, reproducibility, evenness of coverage at annotated transcripts, performance at 50 - and 30 -ends and cross-platform consistency. In ...
... RNA-seq methods, some of which are described in literature (10): library complexity, the number of unique reads, ribosomal RNA read-count in comparison to total reads, reproducibility, evenness of coverage at annotated transcripts, performance at 50 - and 30 -ends and cross-platform consistency. In ...
Agilent Whole Human Genome Oligo Microarray Kit
... Figures A & B depict Agilent’s proven process for ‘superclustering’ and choosing multiple probes within a GeneBin (gene and associated transcripts). In many cases, more than one consensus region will be selected for a given GeneBin. Figure A demonstrates the selection of consensus regions and probes ...
... Figures A & B depict Agilent’s proven process for ‘superclustering’ and choosing multiple probes within a GeneBin (gene and associated transcripts). In many cases, more than one consensus region will be selected for a given GeneBin. Figure A demonstrates the selection of consensus regions and probes ...
Replication origin plasticity, Taylor-made: inhibition vs
... (segments of amplified chromosomal DNA) had different structures. Surprisingly, one of these cell lines (474) initiated replication at several origins in addition to the previously mapped origin that was preferentially used in two other lines (471 and 422), and frequently initiated at more than one ...
... (segments of amplified chromosomal DNA) had different structures. Surprisingly, one of these cell lines (474) initiated replication at several origins in addition to the previously mapped origin that was preferentially used in two other lines (471 and 422), and frequently initiated at more than one ...
SAY IT WITH DNA: PROTEIN SYNTHESIS WORKSHEET: Practice
... STEP 2: Figure out the tRNA triplets (codons) that would fit the mRNA triplets (letter by letter). STEP 3: Look up each tRNA codon in the tRNA Dictionary (below), and find the corresponding symbol and amino acid abbreviation for that codon. Record that one-letter symbol (and its amino acid) below ea ...
... STEP 2: Figure out the tRNA triplets (codons) that would fit the mRNA triplets (letter by letter). STEP 3: Look up each tRNA codon in the tRNA Dictionary (below), and find the corresponding symbol and amino acid abbreviation for that codon. Record that one-letter symbol (and its amino acid) below ea ...
Recombinant Materials Form
... transferred to another host by well-established physiological means? 14. Will the experiment include recombinant or synthetic nucleic acids that consist entirely of nucleic acids from a eukaryotic host including its chloroplasts mitochondria, or plasmids (but excluding viruses) when propagated only ...
... transferred to another host by well-established physiological means? 14. Will the experiment include recombinant or synthetic nucleic acids that consist entirely of nucleic acids from a eukaryotic host including its chloroplasts mitochondria, or plasmids (but excluding viruses) when propagated only ...
1 Title: Long-term natural selection affects patterns of
... genes, with estimates near genes almost twice as high as those far from genes. Our results suggest that natural selection shapes patterns of divergence in neutral genomic regions across the great apes, with unique effects on the X and autosomes that can significantly impact estimates of evolutionary ...
... genes, with estimates near genes almost twice as high as those far from genes. Our results suggest that natural selection shapes patterns of divergence in neutral genomic regions across the great apes, with unique effects on the X and autosomes that can significantly impact estimates of evolutionary ...
Genomic library

A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.