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Jump to Terms beginning with: A B Ca-Cn Co
Jump to Terms beginning with: A B Ca-Cn Co

... autosomal set The non-sex chromosomes consisting of one from each homologous pair in a diploid species. ...
Microsoft Word (Chapter 3) - DORAS
Microsoft Word (Chapter 3) - DORAS

... siderophores, haem and haemoglobin. After incubation for 24 – 48 hours at 30oC, halos of growth appear around wells where the test solutions are being utilised. Ferric chloride is generally added to one of the wells for each bioassay as a positive control. Strains with the ability to produce and uti ...
Detecting polygenic selection in marine populations by combining
Detecting polygenic selection in marine populations by combining

... fundamental objective. The application of next-generation sequencing technologies in ecological genomics has opened new perspectives for understanding how adaptation proceeds in nature (Davey et al. 2011; Savolainen et al. 2013). By providing genome-wide coverage ...
Transcripts of the MHM region on the chicken Z chromosome
Transcripts of the MHM region on the chicken Z chromosome

... Recently, the ASW/Wpkci gene was found in the distal non-hetrochromatic region on the short arm of the chicken W chromosome (O'Neill et al. 2000, Hori et al. 2000). Wpkci is actively transcribed during the early embryonic stage and encodes an altered form of PKCI (protein kinase C interacting protei ...
DNA - CS.Duke
DNA - CS.Duke

... The   code   in   the   class  DNABenchMark   can   be   used   to   benchmark   the  cutAndSplice  method.   The   code   given   to   you   will   pop-­‐up   a   file-­‐dialog   box   —   when   run   you   can   use   this   to   nav ...
Variation in the size of the oqhcontaining linear
Variation in the size of the oqhcontaining linear

... We have found that the three recently defined Borrelia species associated with Lyme disease have differences in the sizes of their ospA-containing plasmids but not their linear chromosomes. The ospA-containing plasmid from B. burgdorferi was statistically smaller than the corresponding DNA molecules ...
Topic 8 - OoCities
Topic 8 - OoCities

... sister chromatids attached at the centromere. Each two chromosomes of a pair come close together and are crossed at areas called chiasmata. The process of crossing over occurs between the chromatids of the two homologous chromosomes at these points. Recombination is the process that causes the two c ...
Functional genomics in chickens
Functional genomics in chickens

Karyotypes and Karyotyping
Karyotypes and Karyotyping

... arrangement, number, size, shape, or other characteristics of the chromosomes. ...
AP & Regents Biology
AP & Regents Biology

... the time interval between 10 and 20 minutes. c. Account for the differences in oxygen consumption observed between: 1. germinating seeds at 22°C and at 10°C 2. germinating seeds and dry seeds. d. Describe the essential features of an experimental apparatus that could be used to measure oxygen consum ...
1. The figure below represents a water molecule. H O H Water
1. The figure below represents a water molecule. H O H Water

Echinomycin binding to alternating AT
Echinomycin binding to alternating AT

... To check the effect of the central CpG step on the binding to (AT)n we have performed similar footprinting experiments on DNA fragments containing central GG steps. Figure 4 presents DNase I digestion and DEPC modification of a fragment containing the sequence (AT)15GG(AT)6 in the presence of echino ...
FLUORESCENT PROTEIN IN Escherichia coli
FLUORESCENT PROTEIN IN Escherichia coli

Electrophoresis Systems for Nucleic Acids
Electrophoresis Systems for Nucleic Acids

Amphioxus
Amphioxus

... floridae genome. Both are located in the negative strand of the B. floridae genome scaffold 398. Exact location in pair bases with respect to the scaffold sequence is represented below each gene. The distance between both genes, as well as their size, are also indicated. Exons are shown in gray, and ...
The genomic landscape of meiotic crossovers and gene
The genomic landscape of meiotic crossovers and gene

Title: Evolution of dosage compensation in Anolis carolinensis, a
Title: Evolution of dosage compensation in Anolis carolinensis, a

... in sequencing coverage and quality among replicates, but also provided five biologically and technically independent estimates of diversity among these individuals. For these analyses, we focused exclusively on biallelic SNPs, to which we applied a series of filters, removing sites at which the sequ ...
Genes Identified by Visible Mutant Phenotypes Show Increased Bias
Genes Identified by Visible Mutant Phenotypes Show Increased Bias

Lecture Notes
Lecture Notes

Complete Laboratory PDF
Complete Laboratory PDF

... the principle that the closer two genes or markers are located to one another on a chromosome, the greater the chance that they will be inherited together as a unit (linked). Conversely, locations farther apart on the chromosome are more likely to be separated by chromosome recombination during meio ...
Marker Saturation and Construction of a High
Marker Saturation and Construction of a High

Original Article:
Original Article:

Directed evolution - School of Chemical Sciences
Directed evolution - School of Chemical Sciences

Huntingtin grabs a hammer: DNA repair in HD
Huntingtin grabs a hammer: DNA repair in HD

... Truant’s team, helmed by postdoctoral researcher Tam Maiuri, used an innovative method to pursue their hypothesis, using molecules called “chromobodies.” These can attach to specific protein targets and emit fluorescent light, illuminating working proteins that can be tracked under a microscope. In ...
Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution
Molecular Characterization of a Chromosomal Rearrangement Involved in the Adaptive Evolution

... have found, however, that the SSU1-R allele, which confers sulfite resistance to yeast cells, is the product of a reciprocal translocation between chromosomes VIII and XVI due to unequal crossing-over mediated by microhomology between very short sequences on the 5⬘ upstream regions of the SSU1 and E ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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