Quiz 3 review sheet
... • Describe the conditions that, if they change, will have an impact on allele frequencies over time (Hardy Weinberg Equilibrium) • Explain how and why non-coding regions are used for DNA profiling • Interpret data from genome screening • Describe the conditions that are important for the “Hardy Wein ...
... • Describe the conditions that, if they change, will have an impact on allele frequencies over time (Hardy Weinberg Equilibrium) • Explain how and why non-coding regions are used for DNA profiling • Interpret data from genome screening • Describe the conditions that are important for the “Hardy Wein ...
Lecture 11 - Lectures For UG-5
... • Because shearing is random and performed on multiple copies of DNA, each portion of the genome should be represented multiple times in different fragment frames. • In other words, the sequences of the fragments (and thus the reads) should overlap. • After sequencing, the overlapping reads are asse ...
... • Because shearing is random and performed on multiple copies of DNA, each portion of the genome should be represented multiple times in different fragment frames. • In other words, the sequences of the fragments (and thus the reads) should overlap. • After sequencing, the overlapping reads are asse ...
lecture2
... This graphic shows the "recognition helix" to which the CAP protein (a homodimer) binds in the lac operon of E. coli. The DNA of many transposons is flanked by inverted repeats such as this one: 5' GGCCAGTCACAATGG..~400 nt..CCATTGTGACTGGCC 3' 3' CCGGTCAGTGTTACC..~400 nt..GGTAACACTGACCGG 5' Inverted ...
... This graphic shows the "recognition helix" to which the CAP protein (a homodimer) binds in the lac operon of E. coli. The DNA of many transposons is flanked by inverted repeats such as this one: 5' GGCCAGTCACAATGG..~400 nt..CCATTGTGACTGGCC 3' 3' CCGGTCAGTGTTACC..~400 nt..GGTAACACTGACCGG 5' Inverted ...
BAC vectors (Bacterial Artificial Chromosome)
... P1 phage replicon can also be placed into a plasmid to create so called PAC vectors (P1 artificial chromosmes). The P1 phage head can accommodate larger DNA molecules (~100 kb) than the λ head. ...
... P1 phage replicon can also be placed into a plasmid to create so called PAC vectors (P1 artificial chromosmes). The P1 phage head can accommodate larger DNA molecules (~100 kb) than the λ head. ...
Slide 1
... Why is determining an organism’s genome sequence important? The genome sequences of other species have many other uses. The genomes of organisms used in farming, from rice and wheat to pigs and cattle, are being sequenced to help to breed improved strains. But the vast majority of the many thousands ...
... Why is determining an organism’s genome sequence important? The genome sequences of other species have many other uses. The genomes of organisms used in farming, from rice and wheat to pigs and cattle, are being sequenced to help to breed improved strains. But the vast majority of the many thousands ...
notes - Southington Public Schools
... allowing visual proof that cells in a sample or organism got the new gene being studied. The Human Genome Genome = the complete set of genes for an organism. The human genome contains approximately 21,000-23,000 protein coding genes, made up of about 3 billion base pairs. (ATACGACCTG, etc., 3 billio ...
... allowing visual proof that cells in a sample or organism got the new gene being studied. The Human Genome Genome = the complete set of genes for an organism. The human genome contains approximately 21,000-23,000 protein coding genes, made up of about 3 billion base pairs. (ATACGACCTG, etc., 3 billio ...
Applied genetics - questions
... 1 A strain of barley (A) has a high yield of seeds but a long stem which is subject to ‘lodging’ (a flattening of areas of the crop). Another strain (B) has a short, sturdy stem but a lower yield. The genotype of variety A is HHss (high yield, long stem) and the genotype of B is hhSS (low yield, sho ...
... 1 A strain of barley (A) has a high yield of seeds but a long stem which is subject to ‘lodging’ (a flattening of areas of the crop). Another strain (B) has a short, sturdy stem but a lower yield. The genotype of variety A is HHss (high yield, long stem) and the genotype of B is hhSS (low yield, sho ...
Genetic Engineering
... Steps for Plasmid Use 1. Get the DNA for the trait 2. Insert DNA into the plasmid 3. Bacterial transformation 4. Identification of the new trait *Fig 20.4, page 399 ...
... Steps for Plasmid Use 1. Get the DNA for the trait 2. Insert DNA into the plasmid 3. Bacterial transformation 4. Identification of the new trait *Fig 20.4, page 399 ...
molecular genetics unit review
... What are the four ways gene expression is controlled? What is an operon? Describe/explain the 2 main operons (lac, trp) in prokaryotic cells. a) What are mutations? b) What are the different types of mutations and their effects? What are some differences between eukaryotes and prokaryotes? How are g ...
... What are the four ways gene expression is controlled? What is an operon? Describe/explain the 2 main operons (lac, trp) in prokaryotic cells. a) What are mutations? b) What are the different types of mutations and their effects? What are some differences between eukaryotes and prokaryotes? How are g ...
Functional Annotation of Animal Genomes (FAANG)
... • Horse Genome Project (MIT), NHGRI • Chicken Genome Project (Washington University), NHGRI • Although there is no direct involvement with Swine Genome Project, NIH has always been interested in Swine as a model for growth, development, and disease since the pattern of growth and development of pigs ...
... • Horse Genome Project (MIT), NHGRI • Chicken Genome Project (Washington University), NHGRI • Although there is no direct involvement with Swine Genome Project, NIH has always been interested in Swine as a model for growth, development, and disease since the pattern of growth and development of pigs ...
RESEARCH GLOSSARY
... corn genome and other plant genomes. The draft of the corn genome sequence was completed in 2008. The next phase of the project is to apply genomic knowledge to improve plant performance in the field. Scientists are using genotyping as an important tool to develop new products and traits. ...
... corn genome and other plant genomes. The draft of the corn genome sequence was completed in 2008. The next phase of the project is to apply genomic knowledge to improve plant performance in the field. Scientists are using genotyping as an important tool to develop new products and traits. ...
Genetics 2. A typical cell of any organism contains genetic
... typically have two copies of every gene. The two copies (or alleles) of the gene may or may not be identical, and one may be dominant in determining the phenotype while the other is recessive. e. DNA is the genetic material of living organisms, and is located in the chromosomes of each cell. ...
... typically have two copies of every gene. The two copies (or alleles) of the gene may or may not be identical, and one may be dominant in determining the phenotype while the other is recessive. e. DNA is the genetic material of living organisms, and is located in the chromosomes of each cell. ...
Chap5 Genetic Engineering
... This involves the formation of a zygote (a diploid cell) from the fusion of two haploid cells, each having a single set of chromosomes. e.g. some yeasts can exist as haploid (in the forms of and a cells) or diploid (formed by mating of and a cells). The haploid contains 16 linear chromosomes eac ...
... This involves the formation of a zygote (a diploid cell) from the fusion of two haploid cells, each having a single set of chromosomes. e.g. some yeasts can exist as haploid (in the forms of and a cells) or diploid (formed by mating of and a cells). The haploid contains 16 linear chromosomes eac ...
Introduction to Genomics - Department of Microbiology and Plant
... The purpose of this class is to introduce the organization and evolution of genomes through the following topics: Genes and the organization of the nucleus Sequencing of genomes Computational and comparative genomics; Organization and evolution of genomes; SNPs Functional genomics: characterization ...
... The purpose of this class is to introduce the organization and evolution of genomes through the following topics: Genes and the organization of the nucleus Sequencing of genomes Computational and comparative genomics; Organization and evolution of genomes; SNPs Functional genomics: characterization ...
Genetic Engineering
... that can be treated by using proteins produced through genetic engineering ...
... that can be treated by using proteins produced through genetic engineering ...
ppt
... melanogaster. When females heterozygous for these genes were crossed with scute bristled, ruby eyed males, the following classes and numbers of progeny (out of 1000) ...
... melanogaster. When females heterozygous for these genes were crossed with scute bristled, ruby eyed males, the following classes and numbers of progeny (out of 1000) ...
markscheme File
... Award [1 max] for any of the following which refer to the comparison of BR-R and SU-R in graph 2. BR-R performs (slightly) better when combined with SU-R; SU-R performs less well when combined with BR-R; Award [2 max] to a candidate who combines these marks into a single statement: ...
... Award [1 max] for any of the following which refer to the comparison of BR-R and SU-R in graph 2. BR-R performs (slightly) better when combined with SU-R; SU-R performs less well when combined with BR-R; Award [2 max] to a candidate who combines these marks into a single statement: ...
DNA microarray - Creighton Chemistry Webserver
... growth (called crown gall), bacterium contain large Ti plasmid where T DNA moves from bacterium and integrates into plant cell chromosomes, 25 bp repeats and vir genes are essential ...
... growth (called crown gall), bacterium contain large Ti plasmid where T DNA moves from bacterium and integrates into plant cell chromosomes, 25 bp repeats and vir genes are essential ...
Know your molecules organizer
... twisted DNA strand as it unwinds by snipping the strand and resealing it Adds short RNA segments to which DNA polymerase III can attach nucleotides during replication Adds deoxyribonucleotides to the 3’ end of an existing chain Removes RNA primers and replaces them with deoxyribonucleotides Joins Ok ...
... twisted DNA strand as it unwinds by snipping the strand and resealing it Adds short RNA segments to which DNA polymerase III can attach nucleotides during replication Adds deoxyribonucleotides to the 3’ end of an existing chain Removes RNA primers and replaces them with deoxyribonucleotides Joins Ok ...
Supplemental Material
... manipulations were according to SAMBROOK et al. (2001). Isolation of A. nidulans DNA was performed using standard procedures. DNA fragment probes for Southern analysis were labeled with [-32P]dCTP using the RTS Random Primer DNA labeling System kit (Invitrogen, USA). Using Primer Express Version 1. ...
... manipulations were according to SAMBROOK et al. (2001). Isolation of A. nidulans DNA was performed using standard procedures. DNA fragment probes for Southern analysis were labeled with [-32P]dCTP using the RTS Random Primer DNA labeling System kit (Invitrogen, USA). Using Primer Express Version 1. ...
biotech
... Reproductive Cloning Therapeutic cloning treats disease via “replacement cells”, tissues Reproductive cloning copies entire animal Uses? Ethics? ...
... Reproductive Cloning Therapeutic cloning treats disease via “replacement cells”, tissues Reproductive cloning copies entire animal Uses? Ethics? ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.