Development of novel computational tools based on
... phenotype upon a normally avirulent strain (Ochman, 2001). PAIs are highly variable mobile DNA segments present only in one or more strains of a given species. These segments can transfer between environmental microorganisms, across species and even genus boundaries and influence virulence. Genomic ...
... phenotype upon a normally avirulent strain (Ochman, 2001). PAIs are highly variable mobile DNA segments present only in one or more strains of a given species. These segments can transfer between environmental microorganisms, across species and even genus boundaries and influence virulence. Genomic ...
Microfluidic Tools for Systems Biology
... which to construct experiments. It further allows for more precise study of each module as a system with controllable parameters. Favorable economic implications of modularity aside, a similar toolkit consisting of reaction, separation, detection, and valving mechanisms analogous to resistors and tr ...
... which to construct experiments. It further allows for more precise study of each module as a system with controllable parameters. Favorable economic implications of modularity aside, a similar toolkit consisting of reaction, separation, detection, and valving mechanisms analogous to resistors and tr ...
Rapid DNA Extraction from Plant Seeds for PCR
... storage carbohydrates and polyphenols can interfere with successful amplification of DNA prepared from seeds. Until now, cumbersome preparation steps were needed to purify analytical amounts of seed DNA. EPICENTRE’s new QuickExtract™ Seed DNA Extraction Solution facilitates the extraction of PCR-rea ...
... storage carbohydrates and polyphenols can interfere with successful amplification of DNA prepared from seeds. Until now, cumbersome preparation steps were needed to purify analytical amounts of seed DNA. EPICENTRE’s new QuickExtract™ Seed DNA Extraction Solution facilitates the extraction of PCR-rea ...
Epigenetic differences arise during the lifetime of
... (1). The origin of MZ twins is attributed to two or more daughter cells of a single zygote undergoing independent mitotic divisions, leading to independent development and births. They are considered genetically identical, but significant phenotypic discordance between them may exist. This quality i ...
... (1). The origin of MZ twins is attributed to two or more daughter cells of a single zygote undergoing independent mitotic divisions, leading to independent development and births. They are considered genetically identical, but significant phenotypic discordance between them may exist. This quality i ...
Horizontal gene transfer and microbial evolution: Is the Tree-of
... This approach can also be used to successively refine the model. Bayesian statistics use ML analyses to calculate posterior probabilities for trees, clades and evolutionary parameters. Especially MCMC approaches have become very popular in the last year, because they allow to estimate evolutionary p ...
... This approach can also be used to successively refine the model. Bayesian statistics use ML analyses to calculate posterior probabilities for trees, clades and evolutionary parameters. Especially MCMC approaches have become very popular in the last year, because they allow to estimate evolutionary p ...
Repair of Site-Specific DNA Double-Strand Breaks in
... and May, 2003; Reiss, 2003). The GT efficiency can be increased by DSBs in the target sequence (e.g., Fauser et al., 2012; Puchta and Fauser, 2013; Qi et al., 2013). To increase GT in angiosperms in a controlled manner, it is necessary to know the circumstances under which DSBs are repaired along dif ...
... and May, 2003; Reiss, 2003). The GT efficiency can be increased by DSBs in the target sequence (e.g., Fauser et al., 2012; Puchta and Fauser, 2013; Qi et al., 2013). To increase GT in angiosperms in a controlled manner, it is necessary to know the circumstances under which DSBs are repaired along dif ...
Evolution of RH Genes in Hominoids: Characterization of a Gorilla
... of the PCR reaction; a mismatch was introduced into the sequence of primer Gor.CEdir at position 6 from the 5⬘ end. Amplified fragments were separated by size with agarose-gel electrophoresis and were purified on columns (Qiagen, Hilden, Germany). Following purification, PCR fragments were sequenced ...
... of the PCR reaction; a mismatch was introduced into the sequence of primer Gor.CEdir at position 6 from the 5⬘ end. Amplified fragments were separated by size with agarose-gel electrophoresis and were purified on columns (Qiagen, Hilden, Germany). Following purification, PCR fragments were sequenced ...
Enthusiasm mixed with scepticism about single
... Syvånen, Uppsala University, Sweden, and Andres Metspalu, Tartu University, Estonia), or via a ligase assay (Ed Southern, Oxford University, UK) were shown to distinguish SNPs in heterozygote and homozygote form more accurately than hybridisation-based methods. Nonetheless, hybridisation to high de ...
... Syvånen, Uppsala University, Sweden, and Andres Metspalu, Tartu University, Estonia), or via a ligase assay (Ed Southern, Oxford University, UK) were shown to distinguish SNPs in heterozygote and homozygote form more accurately than hybridisation-based methods. Nonetheless, hybridisation to high de ...
Lab 7 - Microbial and Fungal Diversity Part 1 – Microbial Ecology
... The bacteria used in these exercises are not pathogenic (disease-producing); nevertheless, use appropriate techniques and work with care! If a spill occurs, wear disposable gloves, and wipe up the spill with paper towels. Follow this by washing the affected area with soap and water and a disinfecta ...
... The bacteria used in these exercises are not pathogenic (disease-producing); nevertheless, use appropriate techniques and work with care! If a spill occurs, wear disposable gloves, and wipe up the spill with paper towels. Follow this by washing the affected area with soap and water and a disinfecta ...
Evaluation of the Y-Chromosome Structure
... Genomic studies undertaken by Skaletsky et al. (2003) provided evidence of an abundance of intrachromosomal recombination resulting in what was known as the non-recombining region, the NRY, being renamed to the male specific region, the MSY (Skaletsky et al. 2003). ...
... Genomic studies undertaken by Skaletsky et al. (2003) provided evidence of an abundance of intrachromosomal recombination resulting in what was known as the non-recombining region, the NRY, being renamed to the male specific region, the MSY (Skaletsky et al. 2003). ...
Dental plaque biofilm
... Facilitate processing and uptake of nutrients, cross-feeding, removal of harmful metabolic products Development of an appropriate physico-chemical ...
... Facilitate processing and uptake of nutrients, cross-feeding, removal of harmful metabolic products Development of an appropriate physico-chemical ...
Microfluidics Expanding the Frontiers of Microbial Ecology
... research in many fields, from soft-matter physics to chemical engineering, disease diagnostics, and biomedicine. The introduction of microfluidic platforms into the study of microbial ecology is very recent and in many cases still in its infancy, yet existing studies already reveal the great potential ...
... research in many fields, from soft-matter physics to chemical engineering, disease diagnostics, and biomedicine. The introduction of microfluidic platforms into the study of microbial ecology is very recent and in many cases still in its infancy, yet existing studies already reveal the great potential ...
Discovery of MLL1 binding units, their localization to CpG Islands
... Background: Mixed Lineage Leukemia 1 (MLL1) is a mammalian ortholog of the Drosophila Trithorax. In Drosophila, Trithorax complexes transmit the memory of active genes to daughter cells through interactions with Trithorax Response Elements (TREs). However, despite their functional importance, nothin ...
... Background: Mixed Lineage Leukemia 1 (MLL1) is a mammalian ortholog of the Drosophila Trithorax. In Drosophila, Trithorax complexes transmit the memory of active genes to daughter cells through interactions with Trithorax Response Elements (TREs). However, despite their functional importance, nothin ...
ashgPoster2011ver3.pdf
... This example uses the Galaxy server to find potentially functional SNPs within the GWAS catalog. This catalog contains SNPs that are associated genetically with phenotypes; they are tag SNPS, but not necessarily the functional SNP. However, a subset of them could actually be functional, and we will ...
... This example uses the Galaxy server to find potentially functional SNPs within the GWAS catalog. This catalog contains SNPs that are associated genetically with phenotypes; they are tag SNPS, but not necessarily the functional SNP. However, a subset of them could actually be functional, and we will ...
here - MAMMOT
... tells the database that this field has no value). You may find that some of the functions of the viewer will not work properly however (eg no sequence will be available to view if you haven’t entered any). As an example, the file dros1_chip_import.out available from the web site contains the tiling ...
... tells the database that this field has no value). You may find that some of the functions of the viewer will not work properly however (eg no sequence will be available to view if you haven’t entered any). As an example, the file dros1_chip_import.out available from the web site contains the tiling ...
2‐1 GENOMIC ANALYSIS REVEALS MULTIPLE [FeFe
... only the H domain region of each peptide sequence, as defined by a filter used to select appropriate residues from sequence alignments. This subset of amino acids corresponded to the roughly 310 span of residues: C. pasteurianum (P29166) E207-K515 (Total Length = 308) and D. vulgaris (YP_010987) E83 ...
... only the H domain region of each peptide sequence, as defined by a filter used to select appropriate residues from sequence alignments. This subset of amino acids corresponded to the roughly 310 span of residues: C. pasteurianum (P29166) E207-K515 (Total Length = 308) and D. vulgaris (YP_010987) E83 ...
Ledbetter Presentation 8/15/05
... the sensitivity and accuracy of CGH-arrays since we detected 100% of all imbalances (n=17) identified by FISH; ...
... the sensitivity and accuracy of CGH-arrays since we detected 100% of all imbalances (n=17) identified by FISH; ...
巴西橡胶Pto类抗病同源序列的克隆与系统发育重建
... (STK-1 to STK-50), 32 presented uninterrupted open reading frames (ORFs), while the other 18 sequences presented multiple stop codons in all reading frames, and as a result they were not further investigated. Similarity searches of the 32 hevea sequences using the BLASTX algorithm (Altschul et al, 1 ...
... (STK-1 to STK-50), 32 presented uninterrupted open reading frames (ORFs), while the other 18 sequences presented multiple stop codons in all reading frames, and as a result they were not further investigated. Similarity searches of the 32 hevea sequences using the BLASTX algorithm (Altschul et al, 1 ...
Fighting the good cause: meaning, purpose
... intermediates. Nothing structural persists in this process. DNA is ‘copied’ into RNA and then RNA is ‘copied’ into DNA at a new location in the genome (Finnegan 2012). An LTR retrotransposon can serve as a paradigm. In its guise as double-‐‑stranded genom ...
... intermediates. Nothing structural persists in this process. DNA is ‘copied’ into RNA and then RNA is ‘copied’ into DNA at a new location in the genome (Finnegan 2012). An LTR retrotransposon can serve as a paradigm. In its guise as double-‐‑stranded genom ...
Supplement: Sequence analysis of GMR-dRetMEN2B
... AE003818 in 1st intron/alternate exon of drk The P from which flanking genomic sequence was obtained is noted. Sequences were matched to full genomic sequence and insertion sites were mapped. The genomic scaffold segment is indicated. All sequence data shown for the Bloomington lines was generated b ...
... AE003818 in 1st intron/alternate exon of drk The P from which flanking genomic sequence was obtained is noted. Sequences were matched to full genomic sequence and insertion sites were mapped. The genomic scaffold segment is indicated. All sequence data shown for the Bloomington lines was generated b ...
Identification of Human Polymorphisms in the Phenylthio
... haplotype in humans. Since bitter compounds are also often toxic, it is not at all clear what, if any, selective advantage a nontaster phenotype for bitter compounds would have had in ancestral human populations. ...
... haplotype in humans. Since bitter compounds are also often toxic, it is not at all clear what, if any, selective advantage a nontaster phenotype for bitter compounds would have had in ancestral human populations. ...
19.1 Somatostatin Was the First Human Peptide Hormone Produced
... hormones. Diseases can result when an individual is unable to produce these hormones. In 1976, Robert Swanson and Herbert Boyer formed Genentech Inc. The aspiration of this company was to engineer bacteria to synthesize useful products, particularly peptide and polypeptide hormones. Their first cont ...
... hormones. Diseases can result when an individual is unable to produce these hormones. In 1976, Robert Swanson and Herbert Boyer formed Genentech Inc. The aspiration of this company was to engineer bacteria to synthesize useful products, particularly peptide and polypeptide hormones. Their first cont ...
Time – the emerging dimension of plant virus studies
... tabacum itself is a species found only in crops or as a crop fugitive, and is unlikely to have been the original long-term host of TMV. Another virus that has provided some evidence of a sluggish long-term evolutionary rate is turnip yellow mosaic virus (TYMV). Two distinct lineages of the virus are ...
... tabacum itself is a species found only in crops or as a crop fugitive, and is unlikely to have been the original long-term host of TMV. Another virus that has provided some evidence of a sluggish long-term evolutionary rate is turnip yellow mosaic virus (TYMV). Two distinct lineages of the virus are ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.