Chapter 1 A Perspective on Human Genetics
... Shotgun Cloning (used by private project) • Genomic library prepared • No genetic or physical maps are created • Restriction enzymes are used to cut DNA, and overlapping fragments are created • Clones selected at random from each library and sequenced • Assembler software programs organize informati ...
... Shotgun Cloning (used by private project) • Genomic library prepared • No genetic or physical maps are created • Restriction enzymes are used to cut DNA, and overlapping fragments are created • Clones selected at random from each library and sequenced • Assembler software programs organize informati ...
Investigation of the premelanosome protein
... Re-sequencing of parts of the rabbit PMEL gene and identification of intronic polymorphisms To identify polymorphisms that could be useful to evaluate their possible co-segregation with the black-blue spotted phenotype in the F1 families, fragments of the rabbit PMEL gene including 4 out of 11 exons ...
... Re-sequencing of parts of the rabbit PMEL gene and identification of intronic polymorphisms To identify polymorphisms that could be useful to evaluate their possible co-segregation with the black-blue spotted phenotype in the F1 families, fragments of the rabbit PMEL gene including 4 out of 11 exons ...
Phevor Combines Multiple Biomedical Ontologies for
... Phevor does not replace existing prioritization tools; rather, it provides a general means of improving every tool’s performance. As we demonstrate, Phevor substantially improves the accuracy of widely used variant-prioritization tools such as SIFT,18 conservation-based tools such as PhastCons,19 an ...
... Phevor does not replace existing prioritization tools; rather, it provides a general means of improving every tool’s performance. As we demonstrate, Phevor substantially improves the accuracy of widely used variant-prioritization tools such as SIFT,18 conservation-based tools such as PhastCons,19 an ...
Repeat mediated gene duplication in the Drosophila
... arm; the results presented are not affected by whether Muller elements or chromosome arms are used. Duplicated genes were assigned to one of three classes based on the relative position of the paralogs: adjacent, non-adjacent intra-chromosome-arm, or interchromosome-arm. Adjacent duplications have n ...
... arm; the results presented are not affected by whether Muller elements or chromosome arms are used. Duplicated genes were assigned to one of three classes based on the relative position of the paralogs: adjacent, non-adjacent intra-chromosome-arm, or interchromosome-arm. Adjacent duplications have n ...
Section D - Prokaryotic and Eukaryotic Chromosome Structure
... Promoter: The sequence of DNA needed for RNA polymerase to bind to the template and accomplish the initiation reaction; the5’-side (upstream) of the coding region; the short conserved sequence (3) Unwind the DNA helix; For base pairing; Begins at the promoter site (4) Synthesis of the RNA strand at ...
... Promoter: The sequence of DNA needed for RNA polymerase to bind to the template and accomplish the initiation reaction; the5’-side (upstream) of the coding region; the short conserved sequence (3) Unwind the DNA helix; For base pairing; Begins at the promoter site (4) Synthesis of the RNA strand at ...
Sequence analysis of three mitochondrial DNA molecules reveals
... yeasts, others have highly diverged. The two mtDNAs are much more compact than that of S.cerevisiae and contain fewer introns and intergenic sequences, although they have almost the same coding potential. A few genes contain group I introns, but group II introns, otherwise found in S.cerevisiae mtDN ...
... yeasts, others have highly diverged. The two mtDNAs are much more compact than that of S.cerevisiae and contain fewer introns and intergenic sequences, although they have almost the same coding potential. A few genes contain group I introns, but group II introns, otherwise found in S.cerevisiae mtDN ...
Plant LTR-retrotransposons and MITEs: control of
... Arabidopsis thaliana retrotransposons represent only the 4 –8% of the genome, in large genomes like maize they can account for more than 50– 80% of their DNA content (Kumar and Bennetzen, 1999). The copy number of retrotransposons increases with their activity due to their replicative mechanism of t ...
... Arabidopsis thaliana retrotransposons represent only the 4 –8% of the genome, in large genomes like maize they can account for more than 50– 80% of their DNA content (Kumar and Bennetzen, 1999). The copy number of retrotransposons increases with their activity due to their replicative mechanism of t ...
hal.archives-ouvertes.fr
... Over the past few years an increasing number of genomes has been sequenced, revealing that bacterial species harbor a core ...
... Over the past few years an increasing number of genomes has been sequenced, revealing that bacterial species harbor a core ...
Genetic characterization of the mitochondrial DNA - (BORA)
... and ND6), cytochrome c oxidase subunits I – III (COI, COII and COIII), two rRNA genes (12S rRNA and 16S rRNA) and 22 tRNAs. Two copies of tRNA-Lys are present in the mtDNA of L. salmonis, while tRNA-Cys was not identified. Both DNA strands contain coding regions in the salmon louse, in contrast to t ...
... and ND6), cytochrome c oxidase subunits I – III (COI, COII and COIII), two rRNA genes (12S rRNA and 16S rRNA) and 22 tRNAs. Two copies of tRNA-Lys are present in the mtDNA of L. salmonis, while tRNA-Cys was not identified. Both DNA strands contain coding regions in the salmon louse, in contrast to t ...
wsp Gene Sequences from the Wolbachia of Filarial Nematodes
... The PCR products obtained with primers WSPintF and WSPintR from nematode Wolbachia were sequenced directly, and the sequences were aligned to the wsp gene available for arthropod Wolbachia. We also tried to align wsp to the gene sequences available for the major outer membrane proteins of Anaplasma, ...
... The PCR products obtained with primers WSPintF and WSPintR from nematode Wolbachia were sequenced directly, and the sequences were aligned to the wsp gene available for arthropod Wolbachia. We also tried to align wsp to the gene sequences available for the major outer membrane proteins of Anaplasma, ...
Gene Order Form - life
... Bio Basic Inc. will make its best effort to ship genes with a sequence length less than or equal to one thousand base pairs (1000bp), within ten (10) business days of receipt of an order. Bio Basic Inc. will make its best effort to ship genes with a sequence length between one thousand base pairs (1 ...
... Bio Basic Inc. will make its best effort to ship genes with a sequence length less than or equal to one thousand base pairs (1000bp), within ten (10) business days of receipt of an order. Bio Basic Inc. will make its best effort to ship genes with a sequence length between one thousand base pairs (1 ...
Van de Mark, Daniel: The Numerous Caveats of Designing, Implementing, and Interpreting Genome-Wide Association Studies
... guesswork about which candidate regions might harbor causal agents [5]. There are still a number of technical issues that need to be overcome with GWA studies, and it should be noted that the method itself has a number of inherent limitations. On the technical side, studies are only valuable when th ...
... guesswork about which candidate regions might harbor causal agents [5]. There are still a number of technical issues that need to be overcome with GWA studies, and it should be noted that the method itself has a number of inherent limitations. On the technical side, studies are only valuable when th ...
Structural variations in the human genome
... world, perhaps even in the entire universe. Its massive amount of base pairs consisting of a varying number of genes (per organism) contains hereditary information that is used in the development and functioning of an entire organism. In fact, it is hard to imagine life or living without DNA being i ...
... world, perhaps even in the entire universe. Its massive amount of base pairs consisting of a varying number of genes (per organism) contains hereditary information that is used in the development and functioning of an entire organism. In fact, it is hard to imagine life or living without DNA being i ...
PCR: an outstanding method
... Thanks to huge technical and organisational efforts, first viruses and bacteria, then yeasts, plants and animals relinquished the secrets of their genetic material. This accomplishment would have been unthinkable without PCR, which made it possible to prepare large amounts of DNA within a short time ...
... Thanks to huge technical and organisational efforts, first viruses and bacteria, then yeasts, plants and animals relinquished the secrets of their genetic material. This accomplishment would have been unthinkable without PCR, which made it possible to prepare large amounts of DNA within a short time ...
Contribution of IKBKE and IFIH1 gene variants to SLE susceptibility
... gene 50 -region were retained in most cases; whereas they lost their significant P-values when conditioned on each other. These observations indicate that there are two independent association signals for the IKBKE SNVs in our study. One originates from the 50 -region of the gene, which is more prefe ...
... gene 50 -region were retained in most cases; whereas they lost their significant P-values when conditioned on each other. These observations indicate that there are two independent association signals for the IKBKE SNVs in our study. One originates from the 50 -region of the gene, which is more prefe ...
A new ferrochelatase mutation combined with low
... deletion involving nucleotide base pairs 574–589, and results in translation into a truncated protein, is exclusively found in subjects with EPP, but not in the asymptomatic father in this family. Thus it is highly likely that the O4 deletion is responsible for EPP in this family. Our expression stu ...
... deletion involving nucleotide base pairs 574–589, and results in translation into a truncated protein, is exclusively found in subjects with EPP, but not in the asymptomatic father in this family. Thus it is highly likely that the O4 deletion is responsible for EPP in this family. Our expression stu ...
Synonymous codon bias and functional constraint on GC3
... occurring with high frequency also tend to be represented by more codons, thus facilitating degeneracy in the code that might additionally facilitate functional variation in unknown ways. While it is now clear that natural selection acts on synonymous codons (10,11), the exact molecular traits upon ...
... occurring with high frequency also tend to be represented by more codons, thus facilitating degeneracy in the code that might additionally facilitate functional variation in unknown ways. While it is now clear that natural selection acts on synonymous codons (10,11), the exact molecular traits upon ...
Chapter 10
... into fragments of the desired size by physical means, by treatment with restriction enzymes that have infrequent cleavage sites (for example, enzymes such as NotI and SfiI), or by treatment with ordinary restriction enzymes under conditions in which only a fraction of the restriction sites are cleav ...
... into fragments of the desired size by physical means, by treatment with restriction enzymes that have infrequent cleavage sites (for example, enzymes such as NotI and SfiI), or by treatment with ordinary restriction enzymes under conditions in which only a fraction of the restriction sites are cleav ...
Identification of Bacterial Species Using Colony PCR
... did not permit for the bacteria to be sent off for sequencing to see if each group had predicted the correct species of bacterium. Plates streaked with each group’s bacterium were stored in the refrigerator. The goal of my part of the project was to take each group’s bacteria, amplify the 16S rRNA g ...
... did not permit for the bacteria to be sent off for sequencing to see if each group had predicted the correct species of bacterium. Plates streaked with each group’s bacterium were stored in the refrigerator. The goal of my part of the project was to take each group’s bacteria, amplify the 16S rRNA g ...
Chapter 7 Concepts 1. Microbial population death is exponential
... Terminology is especially important when the control of microorganisms is discussed because words like disinfectant and antiseptic often are used loosely. The situation is even more confusing because a particular treatment can either inhibit growth or kill depending on the conditions. The ability to ...
... Terminology is especially important when the control of microorganisms is discussed because words like disinfectant and antiseptic often are used loosely. The situation is even more confusing because a particular treatment can either inhibit growth or kill depending on the conditions. The ability to ...
Slide 1
... Mutations in creA, creB and creC lead to significant carbon catabolite de-repression of cellulase in A. nidulans The role of the CREB/CREC complex is to remove ubiquitin from ...
... Mutations in creA, creB and creC lead to significant carbon catabolite de-repression of cellulase in A. nidulans The role of the CREB/CREC complex is to remove ubiquitin from ...
Antisense derivatives of U7 small nuclear RNA as
... To be effective, splicing-modulating antisense RNAs must accumulate in the nucleoplasm where splicing occurs (see chapter 42 Aartsma Rus). This is why derivatives of U small nuclear RNAs (snRNAs), and in particular of U7 snRNA, have been widely used for this purpose [1]. Apart from the advantage tha ...
... To be effective, splicing-modulating antisense RNAs must accumulate in the nucleoplasm where splicing occurs (see chapter 42 Aartsma Rus). This is why derivatives of U small nuclear RNAs (snRNAs), and in particular of U7 snRNA, have been widely used for this purpose [1]. Apart from the advantage tha ...
Genomic disorders: structural features of the genome can lead to
... gene – how mutations specifically alter DNA and how these changes affect the structure and expression of encoded proteins. Recently, however, the advances of the human genome project and the completion of total genome sequences for yeast and many bacterial species, have enabled investigators to view ...
... gene – how mutations specifically alter DNA and how these changes affect the structure and expression of encoded proteins. Recently, however, the advances of the human genome project and the completion of total genome sequences for yeast and many bacterial species, have enabled investigators to view ...
Preservative Testing – Choice of Challenge Isolates
... challenge, monitors survival over a 4 week period and derives a pass/fail determination-based comparison of microbial reduction observations to established criteria. The primary functional difference between category methods is the inoculum - the microbes used. Drug PET As drug products are typicall ...
... challenge, monitors survival over a 4 week period and derives a pass/fail determination-based comparison of microbial reduction observations to established criteria. The primary functional difference between category methods is the inoculum - the microbes used. Drug PET As drug products are typicall ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.