Transposable element contributions to plant gene and
... altered gene or genome phenotypes at very high frequencies in both germinal and somatic tissues. These elements include the Ac/Ds, Spm/dspm (En/I) and Mutator systems of maize and the Tam elements of snapdragon [39, 65]. Most of these elements range in size from a few hundred bases to about 10 kb. A ...
... altered gene or genome phenotypes at very high frequencies in both germinal and somatic tissues. These elements include the Ac/Ds, Spm/dspm (En/I) and Mutator systems of maize and the Tam elements of snapdragon [39, 65]. Most of these elements range in size from a few hundred bases to about 10 kb. A ...
cbb752-mg-spr10-bioinfo-intro
... applied to bioinformatics, the computational analysis of gene sequences, macromolecular structures, and functional genomics data on a large scale. (Some topics include: ) Sequence alignment, comparative genomics and phylogenetics, biological databases, geometric analysis of protein structure, molecu ...
... applied to bioinformatics, the computational analysis of gene sequences, macromolecular structures, and functional genomics data on a large scale. (Some topics include: ) Sequence alignment, comparative genomics and phylogenetics, biological databases, geometric analysis of protein structure, molecu ...
The Modest Beginnings of One Genome Project
... studies in the basement laboratory of Norman Davidson at California Institute of Technology. ...
... studies in the basement laboratory of Norman Davidson at California Institute of Technology. ...
Visualisation of Reduced-Dimension Microarray Data Using
... such as the application of principal component analysis (PCA) (Raychaudhuri, Stuart and Altman 2000, Misra et al. 2002, Datta 2003), independent component analysis (ICA) (Liao et al. 2002) and singular value decomposition (Wall, Rechsteiner and Rocha 2003). Gene expression data is usually generated ...
... such as the application of principal component analysis (PCA) (Raychaudhuri, Stuart and Altman 2000, Misra et al. 2002, Datta 2003), independent component analysis (ICA) (Liao et al. 2002) and singular value decomposition (Wall, Rechsteiner and Rocha 2003). Gene expression data is usually generated ...
Developing expressed sequence tag libraries and
... a 384-well format) was produced from Rubus idaeus cv. Heritage [31]. ‘Heritage’ is a widely grown, everbearing cultivar with resistance to most common raspberry diseases, and medium to large sized fruit with good color, flavor, firmness and freezing quality [32]. The cDNA library was prepared from t ...
... a 384-well format) was produced from Rubus idaeus cv. Heritage [31]. ‘Heritage’ is a widely grown, everbearing cultivar with resistance to most common raspberry diseases, and medium to large sized fruit with good color, flavor, firmness and freezing quality [32]. The cDNA library was prepared from t ...
Functional analysis of plastid DNA replication origins in tobacco by
... were obtained with each of these plasmids after bombardment of tobacco leaves. Plastome insertion of the aadA sequence at the targeted position was verified by PCR (data not shown). Transplastomic lines were subjected to repeated cycles of vegetative regeneration before Southern analysis. AK1 transf ...
... were obtained with each of these plasmids after bombardment of tobacco leaves. Plastome insertion of the aadA sequence at the targeted position was verified by PCR (data not shown). Transplastomic lines were subjected to repeated cycles of vegetative regeneration before Southern analysis. AK1 transf ...
Validated preCRMs
... DNA). A high RP score for an aligned block of sequences means that the patterns of alignment columns in it are more similar to the patterns observed in aligned CRMs than those seen in aligned ancestral repeats. The number of possible alignment columns is very large and increases exponentially with a ...
... DNA). A high RP score for an aligned block of sequences means that the patterns of alignment columns in it are more similar to the patterns observed in aligned CRMs than those seen in aligned ancestral repeats. The number of possible alignment columns is very large and increases exponentially with a ...
Assessment of the sanitary status of pome fruit crops in Kosovo, with
... while pear is grown to a lesser extent and quince groves are very limited to non-commercial level. In order to maximize their outputs with the least expenses possible, growers were constrained to use their own propagating material. This was accompanied by a lack of knowledge of the existence of dise ...
... while pear is grown to a lesser extent and quince groves are very limited to non-commercial level. In order to maximize their outputs with the least expenses possible, growers were constrained to use their own propagating material. This was accompanied by a lack of knowledge of the existence of dise ...
Classification_Lowy
... living organisms that establishes a tripartite division of all living organisms– bacteria, archaea and eucarya. His work is based on a comparison of 16s ribosomal RNA sequences. These sequences are highly conserved and undergo change at a slow, gradual and consistent rate. They are therefore useful ...
... living organisms that establishes a tripartite division of all living organisms– bacteria, archaea and eucarya. His work is based on a comparison of 16s ribosomal RNA sequences. These sequences are highly conserved and undergo change at a slow, gradual and consistent rate. They are therefore useful ...
PDF - Journal of Genomics
... The RAST Prokaryotic Genome Annotation Server was used to annotate the genes, which implements a ‘highest confidence first’ propagation strategy (see methods) and the GLIMMER-3 gene prediction scheme. A total of 593 putative protein-coding genes and 35 RNAs were identified on the chromosome, as well ...
... The RAST Prokaryotic Genome Annotation Server was used to annotate the genes, which implements a ‘highest confidence first’ propagation strategy (see methods) and the GLIMMER-3 gene prediction scheme. A total of 593 putative protein-coding genes and 35 RNAs were identified on the chromosome, as well ...
MS Word document - Sequence Ontology
... transcripts. There is a time difference between primary and processed transcripts, that cannot be captured by either isa or part_of relationships. For example, in the current model, cap is part_of mRNA is part_of processed_transcript is part_of transcript is part_of gene. BUT cap is not part of a ge ...
... transcripts. There is a time difference between primary and processed transcripts, that cannot be captured by either isa or part_of relationships. For example, in the current model, cap is part_of mRNA is part_of processed_transcript is part_of transcript is part_of gene. BUT cap is not part of a ge ...
Typing and genome comparison of Neisseria
... oligonucleotides 28-32 nucleotides in length, revealed that the applied hybridisation protocols were highly specific. However, despite of several optimisation steps, the rate of misidentification of oligonucleotides remained >1.8% in consecutive validation experiments using arrays representing the g ...
... oligonucleotides 28-32 nucleotides in length, revealed that the applied hybridisation protocols were highly specific. However, despite of several optimisation steps, the rate of misidentification of oligonucleotides remained >1.8% in consecutive validation experiments using arrays representing the g ...
Population genomics of human gene expression
... complex and monogenic diseases. There has also been an explosion of genome-wide studies examining the genetic basis of complex diseases by exploring the effects of genetic variation such as SNPs4–7 and copy number variants8–10, some of which are clearly in noncoding regions of the genome4–7,11. Tech ...
... complex and monogenic diseases. There has also been an explosion of genome-wide studies examining the genetic basis of complex diseases by exploring the effects of genetic variation such as SNPs4–7 and copy number variants8–10, some of which are clearly in noncoding regions of the genome4–7,11. Tech ...
Recombinant DNA Technology
... 2. Once a gene is sequenced, it can be used in recombinant DNA techniques. Sequencing is a technique used to determine the order of genetic information in DNA. For example the sequence of a gene might begin as C A T A T G. One of the first genes sequenced was the gene that codes for insulin, a hormo ...
... 2. Once a gene is sequenced, it can be used in recombinant DNA techniques. Sequencing is a technique used to determine the order of genetic information in DNA. For example the sequence of a gene might begin as C A T A T G. One of the first genes sequenced was the gene that codes for insulin, a hormo ...
A GO annotation is
... An ontology formally represents knowledge as a set of concepts within a domain, and the relationships among those concepts. It can be used to reason about the entities within that domain and may be used to describe the domain. A Controlled vocabularies provide a way to organize knowledge for subseq ...
... An ontology formally represents knowledge as a set of concepts within a domain, and the relationships among those concepts. It can be used to reason about the entities within that domain and may be used to describe the domain. A Controlled vocabularies provide a way to organize knowledge for subseq ...
Local Similarity in Evolutionary Rates Extends over Whole
... same chromosome. This resulted in a final data set of 1,311 autosomal and 67 X-linked human-rodent orthologs with known positions on the genetic mouse map. We also obtained physical positions on the October 7, 2000, build of the human genome (http://genome.ucsc.edu). (On average, 1.3 cM on human chr ...
... same chromosome. This resulted in a final data set of 1,311 autosomal and 67 X-linked human-rodent orthologs with known positions on the genetic mouse map. We also obtained physical positions on the October 7, 2000, build of the human genome (http://genome.ucsc.edu). (On average, 1.3 cM on human chr ...
Supplementary materials
... To see if the large turnover of NMD candidates between humans and mice are caused by mutations at the stop codons, we checked whether the mouse orthologous stop codon exists for each human NMD stop codon. We did this using two types of alignments. First, we aligned the human and mouse orthologous ex ...
... To see if the large turnover of NMD candidates between humans and mice are caused by mutations at the stop codons, we checked whether the mouse orthologous stop codon exists for each human NMD stop codon. We did this using two types of alignments. First, we aligned the human and mouse orthologous ex ...
Part 2 - Laboratory of Aquaculture & Artemia Reference Center
... infancy and a complete genomic analysis is needed. Artemia has proved to be a potential model for classical genetic studies in the past and molecular level approaches are yet to be iniated such as functional genomics, population genomics etc. Artemia shows well developed gene regulatory system for i ...
... infancy and a complete genomic analysis is needed. Artemia has proved to be a potential model for classical genetic studies in the past and molecular level approaches are yet to be iniated such as functional genomics, population genomics etc. Artemia shows well developed gene regulatory system for i ...
Slides PPT
... • When you want to identify or distinguish one organism from another, such as in forensic testing, you investigate the genome. ...
... • When you want to identify or distinguish one organism from another, such as in forensic testing, you investigate the genome. ...
Analyzis of DNA Copy Number Aberrations with Chipster
... The output from the tool is a big table with large number of columns. Usually there is no need to deal with these manually, but for information’s sake they are as follows: columns labeled chip.* contain the original microarray log ratios, segmented.* contain segmented log ratios, flag.* contain copy ...
... The output from the tool is a big table with large number of columns. Usually there is no need to deal with these manually, but for information’s sake they are as follows: columns labeled chip.* contain the original microarray log ratios, segmented.* contain segmented log ratios, flag.* contain copy ...
Choosing a Cloning Vector
... 2.1.2. λ Vectors (see Chapter 18) The bacteriophage λ genome comprises 48,502 bp. On entering the host cell, the phage adopts one of two life cycles: lytic growth or lysogeny. In lytic growth, approx 100 new virions are synthesized and packaged before lysing the host cell, releasing the progeny phag ...
... 2.1.2. λ Vectors (see Chapter 18) The bacteriophage λ genome comprises 48,502 bp. On entering the host cell, the phage adopts one of two life cycles: lytic growth or lysogeny. In lytic growth, approx 100 new virions are synthesized and packaged before lysing the host cell, releasing the progeny phag ...
FAQs (frequently asked questions) Q.1 What are plasmids? Ans
... HindIII and CIa I lie within the promoter of the tetracycline resistance gene; and three sites for PstI, PvuI and Sca l lie within the β-lactamase gene. Cloning of a DNA fragment into any of these 11 sites results in the insertional inactivation of either one of the antibiotic resistance ...
... HindIII and CIa I lie within the promoter of the tetracycline resistance gene; and three sites for PstI, PvuI and Sca l lie within the β-lactamase gene. Cloning of a DNA fragment into any of these 11 sites results in the insertional inactivation of either one of the antibiotic resistance ...
PHAR2811 Dale`s lecture 7 The Transcriptome Definitions: Genome
... • The 3’ end of the tRNAs all have a CCA, some of which are attached after cleavage (some have the sequence encoded in the DNA). The attachment is done by a special enzyme. • The CCA is important as this is where the amino acid is attached. • Several of the bases e.g. pseudouracils in tRNA molecules ...
... • The 3’ end of the tRNAs all have a CCA, some of which are attached after cleavage (some have the sequence encoded in the DNA). The attachment is done by a special enzyme. • The CCA is important as this is where the amino acid is attached. • Several of the bases e.g. pseudouracils in tRNA molecules ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.