... disease-causing bacteria in the ocean. Biofilms transported on ships, the shells of marine animals such as lobsters, or on microscopic copepods can help spread and transmit pathogens such as cholera. Biofilms are detrimental in other ways. They foul and clog water pipes. They form the substrate on w ...
Precise insertion and guided editing of higher plant
... sequences. No off-target mutations were detected at these genomic loci. The FnCpf1 targeted integration experiment into CAO1 site #1 (GE0001-R) was repeated on a larger scale of transformation screening to demonstrate the efficacy of the FnCpf1 nuclease to generate targeted gene insertions and the r ...
... sequences. No off-target mutations were detected at these genomic loci. The FnCpf1 targeted integration experiment into CAO1 site #1 (GE0001-R) was repeated on a larger scale of transformation screening to demonstrate the efficacy of the FnCpf1 nuclease to generate targeted gene insertions and the r ...
PowerPoint-Präsentation - European Bioinformatics Institute
... • Seven dedicated research groups aim to understand biology through new approaches to interpreting biological data ...
... • Seven dedicated research groups aim to understand biology through new approaches to interpreting biological data ...
Slide - Gerstein Lab
... Fritchman, J. L., Fuhrmann, J. L., Geoghagen, N. S. M., Gnehm, C. L., McDonald, L. A., ...
... Fritchman, J. L., Fuhrmann, J. L., Geoghagen, N. S. M., Gnehm, C. L., McDonald, L. A., ...
Tutorial - Ensembl
... ‘known protein coding’ genes. When a transcript matches back to a known identifier in a public database such as UniProtKB or NCBI RefSeq, it is assigned a ‘known status’. A ‘novel’ ...
... ‘known protein coding’ genes. When a transcript matches back to a known identifier in a public database such as UniProtKB or NCBI RefSeq, it is assigned a ‘known status’. A ‘novel’ ...
Determination of primary structure
... We just went through the CHEMICAL and PHYSICAL. The BIOINFORMATICAL method requires information from chemical or physical, but only a limited amount of sequence. Example: a sequence of 6 AA is only possible as one of 206 possible hexa-peptide sequences (1 of 64 x 106). There are no more than 50,000 ...
... We just went through the CHEMICAL and PHYSICAL. The BIOINFORMATICAL method requires information from chemical or physical, but only a limited amount of sequence. Example: a sequence of 6 AA is only possible as one of 206 possible hexa-peptide sequences (1 of 64 x 106). There are no more than 50,000 ...
REPORT C-Terminal Deletions in the ALAS2 Gene Lead to Gain of
... fluorescent ddNTPs (BigDye) and an ABI Prism 3130XL Genetic Analyzer (PE Biosystems, Warrington, UK). We confirmed the presence or absence of mutations by sequencing both strands. Genotyping with FECH intragenic singlenucleotide polymorphisms (SNPs),4 and microsatellite markers for FECH7 and ALAS2 was ...
... fluorescent ddNTPs (BigDye) and an ABI Prism 3130XL Genetic Analyzer (PE Biosystems, Warrington, UK). We confirmed the presence or absence of mutations by sequencing both strands. Genotyping with FECH intragenic singlenucleotide polymorphisms (SNPs),4 and microsatellite markers for FECH7 and ALAS2 was ...
Information Encoding in Biological Molecules: DNA and
... • Saw gene orthology, DNA homology • Other view is SyntenyView • Also access comparative genomics through EnsMart Lecture/Lab 7.3 ...
... • Saw gene orthology, DNA homology • Other view is SyntenyView • Also access comparative genomics through EnsMart Lecture/Lab 7.3 ...
Polymerase Chain Reaction (PCR) - Department of Environmental
... The polymerase chain reaction (PCR) is an enzymatic process that allows for the detection of specific genes within an environmental DNA sample. PCR utilizes short, user defined DNA sequences called oligonucleotide primers, the sequence of which are complementary to target regions of genes known to e ...
... The polymerase chain reaction (PCR) is an enzymatic process that allows for the detection of specific genes within an environmental DNA sample. PCR utilizes short, user defined DNA sequences called oligonucleotide primers, the sequence of which are complementary to target regions of genes known to e ...
- Philsci
... represented by the root of the tree and represents the shared ancestor of all other organisms within a particular dendrogram which have branched from that tree, making a new species. The traditional organismal phylogenies fail to preserve this level of detail of genomic phylogenies in two ways. Firs ...
... represented by the root of the tree and represents the shared ancestor of all other organisms within a particular dendrogram which have branched from that tree, making a new species. The traditional organismal phylogenies fail to preserve this level of detail of genomic phylogenies in two ways. Firs ...
Statistical analysis of DNA microarray data
... • Linear (global) – the chips have equal median (or mean) intensity • Intensity-based (Lowess) – the chips have equal medians (means) at all intensity values • Quantile – the chips have identical intensity distribution • Quantile is the “best” in term of normalizing the data to desired distribution, ...
... • Linear (global) – the chips have equal median (or mean) intensity • Intensity-based (Lowess) – the chips have equal medians (means) at all intensity values • Quantile – the chips have identical intensity distribution • Quantile is the “best” in term of normalizing the data to desired distribution, ...
The Genomics of Emerging Infectious Disease
... some identified only recently, as with the bacterium Helicobacter pylori (which causes peptic ulcers and gastric cancer [11]). There is no simple definition of an emerging disease, but it can be loosely described as a disease that is novel in some way—for example, one that displays a change in geogr ...
... some identified only recently, as with the bacterium Helicobacter pylori (which causes peptic ulcers and gastric cancer [11]). There is no simple definition of an emerging disease, but it can be loosely described as a disease that is novel in some way—for example, one that displays a change in geogr ...
bioinformatics
... is that there are typically many such clusters of closely related genes on eukayotic chromosomes (e.g. Kihara and Kanehisa, 2000). Such tandem arrays pose a problem since they can create clumps of points in the matrix in the absence of segmental homology. Detandemization prevents such clumps from ap ...
... is that there are typically many such clusters of closely related genes on eukayotic chromosomes (e.g. Kihara and Kanehisa, 2000). Such tandem arrays pose a problem since they can create clumps of points in the matrix in the absence of segmental homology. Detandemization prevents such clumps from ap ...
Physical Mapping of Important Trait Loci in the Pig
... study has been focused on physical mapping of important trait loci in the pig. The different physical mapping techniques are powerful to construct restriction maps, reveal syntenic groups of genes and to determine the chromosomal location of genes and markers. The aim has also been to expand the num ...
... study has been focused on physical mapping of important trait loci in the pig. The different physical mapping techniques are powerful to construct restriction maps, reveal syntenic groups of genes and to determine the chromosomal location of genes and markers. The aim has also been to expand the num ...
The plots show the decay of LD (y-axis) with physical
... Figure S15 DArT-tags mapping comparison against two reference genomes. The plots show the mapping position in base pairs to the zebra finch reference genome (Warren et al. 2010) on the x-axis against the difference in mapping position compared to the collared flycatcher reference genome (Ellegren e ...
... Figure S15 DArT-tags mapping comparison against two reference genomes. The plots show the mapping position in base pairs to the zebra finch reference genome (Warren et al. 2010) on the x-axis against the difference in mapping position compared to the collared flycatcher reference genome (Ellegren e ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
... as the selective medium method(Alsinaet al., 1994), API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require cons ...
... as the selective medium method(Alsinaet al., 1994), API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require cons ...
XistAR write up
... which can only be transcribed from the WT inactivated Xp. This claim was conclusively determined through sequencing by utilizing known SNPs in the Xist/Tsix sequence. Next, to distinguish parent-of-origin-specific from strainspecific bias in expression, they compared RNAs produced from WT F1 hybrids ...
... which can only be transcribed from the WT inactivated Xp. This claim was conclusively determined through sequencing by utilizing known SNPs in the Xist/Tsix sequence. Next, to distinguish parent-of-origin-specific from strainspecific bias in expression, they compared RNAs produced from WT F1 hybrids ...
The dawn of evolutionary genome engineering
... REFS 10–12) — provides a complementary approach to study some of the notoriously difficult evolutionary problems for two reasons: first, it allows the generation of large mutant libraries across many predefined loci; second, it enables the construction of genomic alterations that do not occur sponta ...
... REFS 10–12) — provides a complementary approach to study some of the notoriously difficult evolutionary problems for two reasons: first, it allows the generation of large mutant libraries across many predefined loci; second, it enables the construction of genomic alterations that do not occur sponta ...
Where Is DNA Found?
... Sir Alec Jeffreys is credited with DNA profiling using RFLP. In September of 1984 after years of work, he saw his first series of blots on an X-ray. The technique was first used in forensics, when in 1985 he was asked by police to confirm the rape confession of 17 year old Richard Buckland, who was ...
... Sir Alec Jeffreys is credited with DNA profiling using RFLP. In September of 1984 after years of work, he saw his first series of blots on an X-ray. The technique was first used in forensics, when in 1985 he was asked by police to confirm the rape confession of 17 year old Richard Buckland, who was ...
Microbial Ecology
... genes, were as previously described [9, 13]. For the dnaK and 16S rRNA genes, one strand of the purified PCR products was sequenced with the respective forward primer using an automatic Applied Biosystems DNAsequencer. Only sequences with a Phred score ≥ 25 were used. Both strands were sequenced for ...
... genes, were as previously described [9, 13]. For the dnaK and 16S rRNA genes, one strand of the purified PCR products was sequenced with the respective forward primer using an automatic Applied Biosystems DNAsequencer. Only sequences with a Phred score ≥ 25 were used. Both strands were sequenced for ...
Whole Exome Re-Sequencing Implicates CCDC38 and
... association with lung function in genome-wide association studies, eleven have been reported to show association with airflow obstruction. Although the main risk factor for COPD is smoking, some individuals are observed to have a high forced expired volume in 1 second (FEV1) despite many years of he ...
... association with lung function in genome-wide association studies, eleven have been reported to show association with airflow obstruction. Although the main risk factor for COPD is smoking, some individuals are observed to have a high forced expired volume in 1 second (FEV1) despite many years of he ...
Indezine Template
... • pH (only a few live in acidic environments) • moisture • food • oxygen • these factors must be favorable if microorganisms are to live • different microorganisms require different conditions for survival • eg. aerobic microorganisms grow best with high levels of oxygen, while anaerobic microorgani ...
... • pH (only a few live in acidic environments) • moisture • food • oxygen • these factors must be favorable if microorganisms are to live • different microorganisms require different conditions for survival • eg. aerobic microorganisms grow best with high levels of oxygen, while anaerobic microorgani ...
Antibiotic resistance genes are carried on plasmids
... transfer a copy of the R-plasmids to other bacteria, making them also multiple antibiotic resistant and able to produce a conjugation pilus. In addition, some exotoxins , such as the tetanus exotoxin and Escherichia coli enterotoxin discussed later in this unit under Bacterial Pathogenicity, are als ...
... transfer a copy of the R-plasmids to other bacteria, making them also multiple antibiotic resistant and able to produce a conjugation pilus. In addition, some exotoxins , such as the tetanus exotoxin and Escherichia coli enterotoxin discussed later in this unit under Bacterial Pathogenicity, are als ...
Using a Single Nucleotide Polymorphism to Predict Bitter
... repeat is a short sequence of DNA that is repeated in a headto-tail fashion at a specific chromosomal locus. Tandem repeats are interspersed throughout the human genome. Some sequences are found at only one site -- a single locus - in the human genome. For many tandem repeats, the number of repeated ...
... repeat is a short sequence of DNA that is repeated in a headto-tail fashion at a specific chromosomal locus. Tandem repeats are interspersed throughout the human genome. Some sequences are found at only one site -- a single locus - in the human genome. For many tandem repeats, the number of repeated ...
Neutral lipid storage disease with subclinical myo
... central nervous system dysfunction manifested by seizures, ataxia, or mental retardation [15–17]. However, the lipid storage is invariably accompanied by mitochondrial proliferation with ragged-red fibers (RRF). The lack of weakness and RRF in the muscle biopsy of our patient was against the diagnosi ...
... central nervous system dysfunction manifested by seizures, ataxia, or mental retardation [15–17]. However, the lipid storage is invariably accompanied by mitochondrial proliferation with ragged-red fibers (RRF). The lack of weakness and RRF in the muscle biopsy of our patient was against the diagnosi ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.