Chapter 3. The Beginnings of Genomic Biology
... Wilkins made the laboratory observations that led to the solution of the structure of DNA. Chargaff determined that there were 4 different nitrogen bases found in DNA molecules; the purines, adenine (A) and guanine G), and the pyrimidines, cytosine (C) and thymine (T), and he purified DNA from a num ...
... Wilkins made the laboratory observations that led to the solution of the structure of DNA. Chargaff determined that there were 4 different nitrogen bases found in DNA molecules; the purines, adenine (A) and guanine G), and the pyrimidines, cytosine (C) and thymine (T), and he purified DNA from a num ...
WheatNet: A genome-scale functional network for hexaploid bread
... To assess WheatNet, we used biological process annotations by agriGO (Du et al., 2010), which are moderately distinct from the dataset used for network training (~38% gene pairs by shared agriGO annotations overlap the training data) and one of the few other large-scale wheat annotation sets availab ...
... To assess WheatNet, we used biological process annotations by agriGO (Du et al., 2010), which are moderately distinct from the dataset used for network training (~38% gene pairs by shared agriGO annotations overlap the training data) and one of the few other large-scale wheat annotation sets availab ...
Gene Order Polymorphism in Yeast
... We would also like to examine a. How transposition of the five genes affects their gene expression b. What the frequency of this rearrangement is among a larger sample of natural yeast strains c. Whether there are any clues as to the transposition mechanism in the sequences in and around the transpo ...
... We would also like to examine a. How transposition of the five genes affects their gene expression b. What the frequency of this rearrangement is among a larger sample of natural yeast strains c. Whether there are any clues as to the transposition mechanism in the sequences in and around the transpo ...
Lecture VII – Prokaryotes – Dr
... which many species obtain energy for generating ATP by oxidizing reduced organic compounds Highly reduced molecule, glucose, serves as original electron donor (ie, molecule is oxidized) and highly oxidized molecule, oxygen, serves as ...
... which many species obtain energy for generating ATP by oxidizing reduced organic compounds Highly reduced molecule, glucose, serves as original electron donor (ie, molecule is oxidized) and highly oxidized molecule, oxygen, serves as ...
Expression systems for industrial Gram
... of proteins into the fermentation medium, which in Bacilli has reached a high level of efficiency [l]. Based on the guanine and cytosine (GC) content of their DNA, Gram-positive bacteria can be divided into two major groups, the low and high GC groups. This dichotomy is confirmed by phylogenetic ana ...
... of proteins into the fermentation medium, which in Bacilli has reached a high level of efficiency [l]. Based on the guanine and cytosine (GC) content of their DNA, Gram-positive bacteria can be divided into two major groups, the low and high GC groups. This dichotomy is confirmed by phylogenetic ana ...
comparing quantitative trait loci and gene expression data
... gene in a tissue not included in the expression study but in which the gene plays a role in the phenotype. In addition, the association between a phenotype and a tissue may depend on ephemeral conditions that may not be present when the tissue was collected for the microarray study or on a small per ...
... gene in a tissue not included in the expression study but in which the gene plays a role in the phenotype. In addition, the association between a phenotype and a tissue may depend on ephemeral conditions that may not be present when the tissue was collected for the microarray study or on a small per ...
Phylogeny, taxonomy, and evolution of the endothelin receptor gene
... rerooted such that their therian and non-therian groups were now both monophyletic. Such rerooting is allowed, given the time reversibility of the WAG + C model (Felsenstein, 2004). The potential functional importance of the identified rate shift and conserved sites was assessed against the known pos ...
... rerooted such that their therian and non-therian groups were now both monophyletic. Such rerooting is allowed, given the time reversibility of the WAG + C model (Felsenstein, 2004). The potential functional importance of the identified rate shift and conserved sites was assessed against the known pos ...
1995 Broad et al: CURRENT STATE OF THE NEW ZEALAND
... subsequently enlarged with the discovery of two other centric fusions, MII (Bruere and Mills 1971) and MI11 (Bruere et al. 1972). Bruere and Chapman (1974) renamed the translocation chromosomes tl,tz and t3, respectively. As this renaming preceded the frrst standardization of the sheep karyotype at ...
... subsequently enlarged with the discovery of two other centric fusions, MII (Bruere and Mills 1971) and MI11 (Bruere et al. 1972). Bruere and Chapman (1974) renamed the translocation chromosomes tl,tz and t3, respectively. As this renaming preceded the frrst standardization of the sheep karyotype at ...
Advances in Environmental Biology Zahra Maryami, Arash Fazeli, Ali-Ashraf Mehrabi
... (GBSSI) is one of the most important determinants of starch synthesis of cereals [7] which are located on the group-7 chromosomes of each genomes [8,9]. In bread wheat (TriticumaestivumL. ssp. aestivum; 2n = 6x = 42, AABBDD), three waxy proteins, one for each genome, have been identified. Each waxy ...
... (GBSSI) is one of the most important determinants of starch synthesis of cereals [7] which are located on the group-7 chromosomes of each genomes [8,9]. In bread wheat (TriticumaestivumL. ssp. aestivum; 2n = 6x = 42, AABBDD), three waxy proteins, one for each genome, have been identified. Each waxy ...
DNA Profiling
... Two major factors affecting reliability: population genetics and genetic statistics Humans have thousands of RFLP loci or DNA segments that can be selected and used for fingerprinting analysis Can depend on demographic factors such as ethnicity or geographic isolation Humans only differ in about 1-5 ...
... Two major factors affecting reliability: population genetics and genetic statistics Humans have thousands of RFLP loci or DNA segments that can be selected and used for fingerprinting analysis Can depend on demographic factors such as ethnicity or geographic isolation Humans only differ in about 1-5 ...
A Tree of Life Based on Protein Domain Organizations
... We primarily adopted the Pfam domains for construction of a repertoire of protein domain organizations. Although the SCOP domains have been commonly used in the domain contents methods (Gerstein 1998; Wolf et al. 1999; Lin and Gerstein 2000; Caetano-Anolles G and Caetano-Anolles D 2003; Winstanley e ...
... We primarily adopted the Pfam domains for construction of a repertoire of protein domain organizations. Although the SCOP domains have been commonly used in the domain contents methods (Gerstein 1998; Wolf et al. 1999; Lin and Gerstein 2000; Caetano-Anolles G and Caetano-Anolles D 2003; Winstanley e ...
Comparative Genetic Mapping Revealed Powdery Mildew
... Molecular markers are powerful tools to identify parts of DNA that are located near a gene of interest. Restriction fragment length polymorphisms (RFLP), simple sequence repeats (SSR), random amplified polymorphisms DNA (RAPD), sequence tagged site (STS) and amplified fragment length polymorphisms ( ...
... Molecular markers are powerful tools to identify parts of DNA that are located near a gene of interest. Restriction fragment length polymorphisms (RFLP), simple sequence repeats (SSR), random amplified polymorphisms DNA (RAPD), sequence tagged site (STS) and amplified fragment length polymorphisms ( ...
A virulence-associated gene microarray: a tool for
... of Cy3-labelled test strain were dried. The two probes were mixed together by resuspension in 18 ml distilled water. The target was denatured by heating at 95 uC for 3 min and allowed to cool before the addition of 18 ml genHYB hybridization buffer (Genetix). The hybridization mixture was warmed at ...
... of Cy3-labelled test strain were dried. The two probes were mixed together by resuspension in 18 ml distilled water. The target was denatured by heating at 95 uC for 3 min and allowed to cool before the addition of 18 ml genHYB hybridization buffer (Genetix). The hybridization mixture was warmed at ...
milova_032405_glass
... Database for Analysis of Microarrays containes all information about our arrays, cDNA and oligo sets Sequences annotation is updated and integrated into the database Web interface of the database makes it easy to search for a particular gene, synonyms, map location, function, etc… Easy to use ...
... Database for Analysis of Microarrays containes all information about our arrays, cDNA and oligo sets Sequences annotation is updated and integrated into the database Web interface of the database makes it easy to search for a particular gene, synonyms, map location, function, etc… Easy to use ...
Automated Constraint-Based Nucleotide Sequence Selection for
... complementary strand even though they are not perfectly matched with the template. Because the primers need to possess mismatches relative to the desired binding site in the template, they are inherently less stable than perfectly matched primers. In order to be successfully incorporated, they need ...
... complementary strand even though they are not perfectly matched with the template. Because the primers need to possess mismatches relative to the desired binding site in the template, they are inherently less stable than perfectly matched primers. In order to be successfully incorporated, they need ...
Phytozome Tutorial from David Goodstein
... evolutionary history of each gene family, and identify closely related families (via shared functional annotation or consensus sequence similarity). The site also provides direct and uniform access to much of the information associated with the individual genomes (e.g, annotations, deflines, functio ...
... evolutionary history of each gene family, and identify closely related families (via shared functional annotation or consensus sequence similarity). The site also provides direct and uniform access to much of the information associated with the individual genomes (e.g, annotations, deflines, functio ...
Heritable genome-wide variation of gene expression and promoter methylation between
... Background: Variations in gene expression, mediated by epigenetic mechanisms, may cause broad phenotypic effects in animals. However, it has been debated to what extent expression variation and epigenetic modifications, such as patterns of DNA methylation, are transferred across generations, and the ...
... Background: Variations in gene expression, mediated by epigenetic mechanisms, may cause broad phenotypic effects in animals. However, it has been debated to what extent expression variation and epigenetic modifications, such as patterns of DNA methylation, are transferred across generations, and the ...
Unity and diversity in the metabolism of micro-organisms
... lecture on some biochemical subject before the Netherlands’ Chemical So- that in so many cases the presence of ciety, I gladly accepted because a microbes is forced upon us by the true microbiologist may never pass very fact that we observe chemical up the opportunity to contribute to changes. When ...
... lecture on some biochemical subject before the Netherlands’ Chemical So- that in so many cases the presence of ciety, I gladly accepted because a microbes is forced upon us by the true microbiologist may never pass very fact that we observe chemical up the opportunity to contribute to changes. When ...
Studies on Chlamydomonas Chloroplast Transformation: Foreign
... modulate gene expression in chloroplasts. Recently, Sanford and co-workers (Klein et al., 1987) have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have m ...
... modulate gene expression in chloroplasts. Recently, Sanford and co-workers (Klein et al., 1987) have developed a DNA transfer technique that relies upon bombardment of recipient cells with high-velocity tungsten microprojectiles coated with DNA. Using this delivery system, Klein et al. (1988) have m ...
Symbiotic DNA in eukaryotic genomes
... evolutionary flexibility. Transposition in experimental popuBox 1. Transposons: cut-and-paste elements lations can increase the variability of metabolic and quantitative trait&l4 and consequently the rate of response to Transposons move by excision from a chromosome, followed by reinsertion into a n ...
... evolutionary flexibility. Transposition in experimental popuBox 1. Transposons: cut-and-paste elements lations can increase the variability of metabolic and quantitative trait&l4 and consequently the rate of response to Transposons move by excision from a chromosome, followed by reinsertion into a n ...
Anopheles gambiae APL1 Is a Family of Variable LRR Proteins
... (targeting all 3 genes) permitted significantly greater oocyst development than the other treatments (asterisk, p,0.05 by Dunn’s Multiple Comparison after Kruskal Wallis one way ANOVA on ranks). Infections of APL1C and APL1 silenced mosquitoes were not different from each other, indicating that the ...
... (targeting all 3 genes) permitted significantly greater oocyst development than the other treatments (asterisk, p,0.05 by Dunn’s Multiple Comparison after Kruskal Wallis one way ANOVA on ranks). Infections of APL1C and APL1 silenced mosquitoes were not different from each other, indicating that the ...
Mechanisms of Nucleolar Dominance in Animals and Plants
... species' promoter when added to an extract from another species (l 9, 20). This factor has been extensively purified from human cells, and antibodies directed against it stain the nudeolus exclusively (23). These observations suggest that in crosses between distantly related species, where the polym ...
... species' promoter when added to an extract from another species (l 9, 20). This factor has been extensively purified from human cells, and antibodies directed against it stain the nudeolus exclusively (23). These observations suggest that in crosses between distantly related species, where the polym ...
Potato Genome Sequencing Consortium: Final
... then doubling it using tissue culture techniques. Phureja types are virtually identical to conventional potatoes in their genome sequence and the use of DM made the task of sequencing the genome much simpler. The genome sequencing process itself is quite simple nowadays, thanks to the recent develop ...
... then doubling it using tissue culture techniques. Phureja types are virtually identical to conventional potatoes in their genome sequence and the use of DM made the task of sequencing the genome much simpler. The genome sequencing process itself is quite simple nowadays, thanks to the recent develop ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.