Nuclear Genes That Encode Mitochondrial Proteins
... sion of particular subgenomic DNA molecules to nearly undetectable levels during plant development. This process, first discovered in maize (Small et al., 1987), appears to be widespread in plants and may constitute a means of maintaining mitochondrial genetic variation in a silenced but retrievable ...
... sion of particular subgenomic DNA molecules to nearly undetectable levels during plant development. This process, first discovered in maize (Small et al., 1987), appears to be widespread in plants and may constitute a means of maintaining mitochondrial genetic variation in a silenced but retrievable ...
CHARACTERlZATION OF THE ~ 0 CHONDRIA . L DNA MOLECULE
... mitochondrid replication and transcription are not known, comparative analyses of the controI region from various mamindian species have identified regions of sequence consensus and possible functionai importance (Ciayton, 199la; 199 1b). These include the conserved sequence blocks [CSB) (Walberg an ...
... mitochondrid replication and transcription are not known, comparative analyses of the controI region from various mamindian species have identified regions of sequence consensus and possible functionai importance (Ciayton, 199la; 199 1b). These include the conserved sequence blocks [CSB) (Walberg an ...
user guide here
... gVCF is a text file format, stored as a gzip compressed file (*.genome.vcf.gz). Compression is further achieved by joining contiguous non-variant regions with similar properties into single ‘block’ VCF records. To maximize the utility of gVCF, especially for high stringency applications, the proper ...
... gVCF is a text file format, stored as a gzip compressed file (*.genome.vcf.gz). Compression is further achieved by joining contiguous non-variant regions with similar properties into single ‘block’ VCF records. To maximize the utility of gVCF, especially for high stringency applications, the proper ...
Rate Asymmetry After Genome Duplication Causes Substantial
... distance terms include a shared component corresponding to the distance between C. albicans and the common ancestor of the two S. cerevisiae copies. However, it is a useful measure because it avoids making any assumption about whether the speciation between S. kluyveri and S. cerevisiae pre-dates or ...
... distance terms include a shared component corresponding to the distance between C. albicans and the common ancestor of the two S. cerevisiae copies. However, it is a useful measure because it avoids making any assumption about whether the speciation between S. kluyveri and S. cerevisiae pre-dates or ...
Reconstruction of phylogenetic trees
... DNA of each individual is unique, but differences are small: 1 in 500 to 1000 nucleotides differ between two individuals. Within a population each position in the DNA has a ‘predominant’ nucleotide. dominant nucleotide generations this ‘pre-dominant’-nucleotide p of a Over g position can change by e ...
... DNA of each individual is unique, but differences are small: 1 in 500 to 1000 nucleotides differ between two individuals. Within a population each position in the DNA has a ‘predominant’ nucleotide. dominant nucleotide generations this ‘pre-dominant’-nucleotide p of a Over g position can change by e ...
Simultaneous mutation scanning for gross deletions
... Primers were designed to amplify all 79 exons of the DMD gene (the entire 30 UTR is not covered), two alternative promoters (purkinje and cortical) and two exons of the myelin protein zero gene (MPZ) located at 1q22 to control for whole gene deletions or duplications. In all cases, primers were desi ...
... Primers were designed to amplify all 79 exons of the DMD gene (the entire 30 UTR is not covered), two alternative promoters (purkinje and cortical) and two exons of the myelin protein zero gene (MPZ) located at 1q22 to control for whole gene deletions or duplications. In all cases, primers were desi ...
UNIFR Rusconi 2002
... Basic understanding of 'genes and genomes': what is a gene, how many genes, molecular ...
... Basic understanding of 'genes and genomes': what is a gene, how many genes, molecular ...
Powerpoint template for scientific poster
... for the control of drug-resistant bacteria and cancer stem cells has led to a revived interest in the useful biological activity, highly complex structure and intriguing biosynthetic mechanisms of these compounds for further application purposes. ...
... for the control of drug-resistant bacteria and cancer stem cells has led to a revived interest in the useful biological activity, highly complex structure and intriguing biosynthetic mechanisms of these compounds for further application purposes. ...
A global view of pleiotropy and phenotypically
... groups with more complex phenotype profiles (Figure 2B), but with equally specific functional enrichments as the gene sets constructed from low-pleiotropy mutants. Consistent with recently published results (Parsons et al, 2004), many of the clusters that include conditions with drugs added to the m ...
... groups with more complex phenotype profiles (Figure 2B), but with equally specific functional enrichments as the gene sets constructed from low-pleiotropy mutants. Consistent with recently published results (Parsons et al, 2004), many of the clusters that include conditions with drugs added to the m ...
Full text in pdf - International Microbiology
... DNA-DNA hybridization has the disadvantage that it does not distinguish the differences due to genome size or plasmid content. Results may vary largely depending on the hybridization conditions and the methodology used [62]. In addition, it is limited by the availability of strains to be compared, w ...
... DNA-DNA hybridization has the disadvantage that it does not distinguish the differences due to genome size or plasmid content. Results may vary largely depending on the hybridization conditions and the methodology used [62]. In addition, it is limited by the availability of strains to be compared, w ...
Catellibacterium aquatile sp. nov., isolated from fresh water, and
... MgSO4 . 7H2O, 0.3 g NaCl, 15 g agar, pH 7.0] or the strain was stored as glycerol stocks (15 %, w/v) at 280 uC. Growth on several bacteriological media was tested: YP agar, R2A agar, trypticase soy agar (TSA; Difco), nutrient agar, Luria–Bertani (LB) agar and LOLB agar. Abundant growth was observed ...
... MgSO4 . 7H2O, 0.3 g NaCl, 15 g agar, pH 7.0] or the strain was stored as glycerol stocks (15 %, w/v) at 280 uC. Growth on several bacteriological media was tested: YP agar, R2A agar, trypticase soy agar (TSA; Difco), nutrient agar, Luria–Bertani (LB) agar and LOLB agar. Abundant growth was observed ...
Andreas Mock Cancer Research UK Cambridge Institute, University
... 1 Assembly and preprocessing of TCGA RNAseq data Melanoma RNAseq data for the CVE extension were downloaded as expression estimates per gene (RNAseq2 level 3 data) from the TCGA data portal. Please note that the TCGA Data portal is no longer operational and all TCGA data now resides at the Genomic D ...
... 1 Assembly and preprocessing of TCGA RNAseq data Melanoma RNAseq data for the CVE extension were downloaded as expression estimates per gene (RNAseq2 level 3 data) from the TCGA data portal. Please note that the TCGA Data portal is no longer operational and all TCGA data now resides at the Genomic D ...
Evolutionary history of the genus Capra
... (ii) previously published and new cytochrome b sequences. For the Y-chromosome phylogeny, we sequenced segments from the amelogenin (AMELY) and zinc Wnger (ZFY) genes from all of the eight wild taxa and from domestic goats (Capra hircus). Phylogenetic analysis of the Y-chromosome data revealed two w ...
... (ii) previously published and new cytochrome b sequences. For the Y-chromosome phylogeny, we sequenced segments from the amelogenin (AMELY) and zinc Wnger (ZFY) genes from all of the eight wild taxa and from domestic goats (Capra hircus). Phylogenetic analysis of the Y-chromosome data revealed two w ...
Planning Microarray Experiments
... Title: Planning Microarray Experiments Abstract: Microarray technology is a powerful tool that allows the study of tens of thousands of genes at once. In the complex microarray experiments, many sources of potential slight disturbances are possible. Statistical design of microarray studies aims at r ...
... Title: Planning Microarray Experiments Abstract: Microarray technology is a powerful tool that allows the study of tens of thousands of genes at once. In the complex microarray experiments, many sources of potential slight disturbances are possible. Statistical design of microarray studies aims at r ...
A global view of pleiotropy and phenotypically derived gene function
... range of pleiotropies by phenotype profile successfully groups genes with common biological functions. The fact that both condition-specific and highly pleiotropic genes can be grouped by common phenotype profiles into gene sets that show significant enrichment for known biological processes suggests th ...
... range of pleiotropies by phenotype profile successfully groups genes with common biological functions. The fact that both condition-specific and highly pleiotropic genes can be grouped by common phenotype profiles into gene sets that show significant enrichment for known biological processes suggests th ...
The DNA sequence and biology of human chromosome 19
... sex-averaged recombination rates are indicated in pink, blue and yellow, respectively. [Adobe PDF, 528 KB] ...
... sex-averaged recombination rates are indicated in pink, blue and yellow, respectively. [Adobe PDF, 528 KB] ...
simultaneous detection of colorectal cancer mutations in stool
... CRC is a disease in which many DNA mutations associated with the process of carcinogenesis have been characterised (4). This characterisation makes it potentially valuable to examine stool for the presence of DNA with different mutations as indicators of both pre-clinical and clinical disease (5). T ...
... CRC is a disease in which many DNA mutations associated with the process of carcinogenesis have been characterised (4). This characterisation makes it potentially valuable to examine stool for the presence of DNA with different mutations as indicators of both pre-clinical and clinical disease (5). T ...
About DNA Ligase The term ligase comes from the latin ligare
... Note: The dilute DNA stain may be saved and reused several times. For best results, reheat the stain before reusing. 15. Add warm distilled or tap water (50 to 55 C) to the staining tray. To accelerate destaining, gently rock the tray. Destain until bands are distinct, with little background color. ...
... Note: The dilute DNA stain may be saved and reused several times. For best results, reheat the stain before reusing. 15. Add warm distilled or tap water (50 to 55 C) to the staining tray. To accelerate destaining, gently rock the tray. Destain until bands are distinct, with little background color. ...
DNA the Crown Jewels 2012
... The Combined DNA Index Sequence A. Use in Forensic Science (CODIS) 1. All 50 states have mandated the collection of DNA from convicted offenders of particular crimes and the establishment of DNA data bases for law enforcement purposes. 2. The CODIS is a computer software program developed by the F ...
... The Combined DNA Index Sequence A. Use in Forensic Science (CODIS) 1. All 50 states have mandated the collection of DNA from convicted offenders of particular crimes and the establishment of DNA data bases for law enforcement purposes. 2. The CODIS is a computer software program developed by the F ...
Gene Conversion as a Source of Nucleotide Diversity in
... both, all except possibly one are consistent with an origin by gene conversion. This result is supported not only by the identity of the polymorphic nucleotides but also by the significant clustering of polymorphic nucleotides within each gene. Positioning of the polymorphisms within derived three-d ...
... both, all except possibly one are consistent with an origin by gene conversion. This result is supported not only by the identity of the polymorphic nucleotides but also by the significant clustering of polymorphic nucleotides within each gene. Positioning of the polymorphisms within derived three-d ...
Modern Techniques in Detection, Identification and
... method was hundreds times faster and 200,000 times more sensitive. Additionally, another DNA fingerprinting identification method was developed based on singleenzyme amplified fragment length polymorphism (SE-AFLP). This method allowed the differentiation of genera, species, and strains of pathogeni ...
... method was hundreds times faster and 200,000 times more sensitive. Additionally, another DNA fingerprinting identification method was developed based on singleenzyme amplified fragment length polymorphism (SE-AFLP). This method allowed the differentiation of genera, species, and strains of pathogeni ...
Arabidopsis thaliana: A Model Plant for Genome Analysis
... Arabidopsis, a small weed in the mustard family that was first chosen as a model genetic organism by Laibach in Europe and later studied in detail by Rédei in the United States (2). The shift toward Arabidopsis gained momentum in the early 1980s with the release of a detailed genetic map (3) and pu ...
... Arabidopsis, a small weed in the mustard family that was first chosen as a model genetic organism by Laibach in Europe and later studied in detail by Rédei in the United States (2). The shift toward Arabidopsis gained momentum in the early 1980s with the release of a detailed genetic map (3) and pu ...
Isolation of insertion elements from Gram
... refers to a close relationship of these bacteria, or indicates that gene transfer events must have happened. IS-Cg and IS-B1 probably have a common ancestor, since both are similar in size, share homologous sequences and generate target site duplications of 8 bp (Fig. lb). In this study we extended ...
... refers to a close relationship of these bacteria, or indicates that gene transfer events must have happened. IS-Cg and IS-B1 probably have a common ancestor, since both are similar in size, share homologous sequences and generate target site duplications of 8 bp (Fig. lb). In this study we extended ...
View PDF - Genetics
... substitution rates (albeit much less pronounced) as well as the accumulation of large indels in these species. In contrast, there was little or no evidence of accelerated sequence evolution in photosynthetic genes in these species. Silene latifolia, S. vulgaris, and Agrostemma githago were chosen ...
... substitution rates (albeit much less pronounced) as well as the accumulation of large indels in these species. In contrast, there was little or no evidence of accelerated sequence evolution in photosynthetic genes in these species. Silene latifolia, S. vulgaris, and Agrostemma githago were chosen ...
Unit 10.1.4 - Measuring Genetic Variation using Molecular Markers
... • Polymorphic. The marker must be polymorphic as it is the polymorphism itself which is measured for genetic diversity studies. However, the level of polymorphism detected can vary depending on the method used to measure it. • Codominant inheritance. The different forms of the marker should be detec ...
... • Polymorphic. The marker must be polymorphic as it is the polymorphism itself which is measured for genetic diversity studies. However, the level of polymorphism detected can vary depending on the method used to measure it. • Codominant inheritance. The different forms of the marker should be detec ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.