Nanosep® Centrifugal Devices - Protocols for Use
... 3K or 100K Nanosep device for 5 to 25 minutes. Recovered samples were quantitated using absorbance at 260 nm. Increased loss of the 500 bp fragment was seen at high speed for the 100K device. Removal of Free Nucleotides from Labeling Reactions Description Synthesis of radioactively-labeled DNA is on ...
... 3K or 100K Nanosep device for 5 to 25 minutes. Recovered samples were quantitated using absorbance at 260 nm. Increased loss of the 500 bp fragment was seen at high speed for the 100K device. Removal of Free Nucleotides from Labeling Reactions Description Synthesis of radioactively-labeled DNA is on ...
Blue cone monochromacy: Causative mutations and associated
... within the opsin gene array, with an inactivating mutation. Family 1 had a single 5′-L–M-3′ hybrid gene, with an inactivating Cys203Arg (C203R) mutation. Family 3 had an array composed of a C203R inactivated 5′-L–M-3′ hybrid gene followed by a second inactive gene. Families 1 and 3 had typical clini ...
... within the opsin gene array, with an inactivating mutation. Family 1 had a single 5′-L–M-3′ hybrid gene, with an inactivating Cys203Arg (C203R) mutation. Family 3 had an array composed of a C203R inactivated 5′-L–M-3′ hybrid gene followed by a second inactive gene. Families 1 and 3 had typical clini ...
Pleistocene Speciation and the Mitochondrial DNA Clock
... estimated rate of substitution is directly related to saturation. The best fit model uses a gamma distribution to incorporate among-site rate heterogeneity and predicts that the actual number of substitutions that have occurred since the divergence of chimpanzees and humans, and since the divergence ...
... estimated rate of substitution is directly related to saturation. The best fit model uses a gamma distribution to incorporate among-site rate heterogeneity and predicts that the actual number of substitutions that have occurred since the divergence of chimpanzees and humans, and since the divergence ...
Gut instincts
... “Currently, the only therapy that causes prolonged weight loss is bariatric surgery, which can result in some adverse effects such as nausea and psychological issues that impact on the patient’s quality of life. Of course, there are also risks associated with surgery. Accordingly, novel methods are ...
... “Currently, the only therapy that causes prolonged weight loss is bariatric surgery, which can result in some adverse effects such as nausea and psychological issues that impact on the patient’s quality of life. Of course, there are also risks associated with surgery. Accordingly, novel methods are ...
Constitutive expression of catABC genes in the aniline
... The aniline-assimilating bacterium Rhodococcus sp. AN-22 was found to constitutively synthesize CatB (cis,cis-muconate cycloisomerase) and CatC (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported CatA (catechol 1,2-dioxygenase). The bacte ...
... The aniline-assimilating bacterium Rhodococcus sp. AN-22 was found to constitutively synthesize CatB (cis,cis-muconate cycloisomerase) and CatC (muconolactone isomerase) in its cells growing on non-aromatic substrates, in addition to the previously reported CatA (catechol 1,2-dioxygenase). The bacte ...
MS26/CYP704B is required for anther and pollen wall
... CYP704B1 catalyzes the in-chain and ω-hydroxylation of fatty acids and is essential for exine biosynthesis [14]. Pollen from cyp704b1 mutant plants lack normal exine, but remain viable and capable of fertilization [14]. The rice OsCYP704B2 gene encodes a long-chain fatty acid hydroxylase capable of ...
... CYP704B1 catalyzes the in-chain and ω-hydroxylation of fatty acids and is essential for exine biosynthesis [14]. Pollen from cyp704b1 mutant plants lack normal exine, but remain viable and capable of fertilization [14]. The rice OsCYP704B2 gene encodes a long-chain fatty acid hydroxylase capable of ...
B ) Revised syllabus with effect from 2007-08
... Biochemistry & Biotechnology as main subject of study of this University or an examination of some other University accepted by the syndicate as equivalent thereto shall be eligible for admission to the Master Degree of this University. 2. Duration of the Course This Course of Study shall be based o ...
... Biochemistry & Biotechnology as main subject of study of this University or an examination of some other University accepted by the syndicate as equivalent thereto shall be eligible for admission to the Master Degree of this University. 2. Duration of the Course This Course of Study shall be based o ...
Enhancers reside in a unique epigenetic environment during early
... The vast majority of enhancers at hypo-methylated loci are not active in any of the embryonic data sets analyzed if we use H3K27ac as a proxy of enhancer activity. To follow up on this observation, we defined two sets of enhancers based on their DNA methylation: hypo-enhancers (<25 % DNA methylation ...
... The vast majority of enhancers at hypo-methylated loci are not active in any of the embryonic data sets analyzed if we use H3K27ac as a proxy of enhancer activity. To follow up on this observation, we defined two sets of enhancers based on their DNA methylation: hypo-enhancers (<25 % DNA methylation ...
Prokaryotes powerpoint
... • OBLIGATE AEROBES(need oxygen to survive) • OBLIGATE ANAEROBES (killed by oxygen) • AEROTOLERANT(don’t use oxygen, but survive it). ...
... • OBLIGATE AEROBES(need oxygen to survive) • OBLIGATE ANAEROBES (killed by oxygen) • AEROTOLERANT(don’t use oxygen, but survive it). ...
not a plastid specific promoter but is also capable of
... of photosynthetically active tissue. However, plastids can also differentiate into other plastid types as chromoplasts or amyloplasts, to assume specialized physiological functions (1). Insight in the regulation of plastid differentiation and biosynthesis is limited. Plastids depend on nuclear genes ...
... of photosynthetically active tissue. However, plastids can also differentiate into other plastid types as chromoplasts or amyloplasts, to assume specialized physiological functions (1). Insight in the regulation of plastid differentiation and biosynthesis is limited. Plastids depend on nuclear genes ...
Introduction - Stellenbosch University
... 2. chromStart - The starting position of the feature in the chromosome or scaffold. The first base in a chromosome is numbered 0. 3. chromEnd - The ending position of the feature in the chromosome or scaffold. The 9 additional optional BED fields are: 4. name - Defines the name of the BED line. This ...
... 2. chromStart - The starting position of the feature in the chromosome or scaffold. The first base in a chromosome is numbered 0. 3. chromEnd - The ending position of the feature in the chromosome or scaffold. The 9 additional optional BED fields are: 4. name - Defines the name of the BED line. This ...
Comparative sequence analysis of the long repeat regions and
... region of some 3500 bp which has not been assigned any protein coding function but which is the major locus of transcription in neurons latently infected with HSV-1, giving rise to R N A species termed latency-associated transcripts (LATs) (Stevens et al., 1987; Rock et al., 1987; Spivack & Fraser, ...
... region of some 3500 bp which has not been assigned any protein coding function but which is the major locus of transcription in neurons latently infected with HSV-1, giving rise to R N A species termed latency-associated transcripts (LATs) (Stevens et al., 1987; Rock et al., 1987; Spivack & Fraser, ...
The ecology of transfer of mobile genetic elements
... activity, resulting in the occurrence of locally enhanced densities of active cells. These sites are often conducive to HGT processes [10], and have been denominated ‘hot’ spots for bacterial gene transfer activity. The conditions in these hot spots that together determine the HGT-conduciveness can ...
... activity, resulting in the occurrence of locally enhanced densities of active cells. These sites are often conducive to HGT processes [10], and have been denominated ‘hot’ spots for bacterial gene transfer activity. The conditions in these hot spots that together determine the HGT-conduciveness can ...
PPTX - Bioinformatics.ca
... • Many coding genes are well annotated with their biological functions. Non-coding regions typically lack such annotation. GREAT assigns biological meaning to a set of non-coding genomic regions by analyzing the annotations of the nearby genes. Thus, it is particularly useful in studying functions o ...
... • Many coding genes are well annotated with their biological functions. Non-coding regions typically lack such annotation. GREAT assigns biological meaning to a set of non-coding genomic regions by analyzing the annotations of the nearby genes. Thus, it is particularly useful in studying functions o ...
Divergent Evolution and Evolution by the Birth-and
... During the past 10 years, the DNA sequences of Vu genes have been determined for many lower vertebrate organisms (see Litman et al. 1993 ) . We can therefore study the pattern of evolution of the Vu gene family on a much longer evolutionary time scale. This type of study is particularly interesting, ...
... During the past 10 years, the DNA sequences of Vu genes have been determined for many lower vertebrate organisms (see Litman et al. 1993 ) . We can therefore study the pattern of evolution of the Vu gene family on a much longer evolutionary time scale. This type of study is particularly interesting, ...
Package `Rsubread` - Bioconductor Mirror, Department of Statistics
... The align function implements the Subread aligner (Liao et al., 2013) that uses a new mapping paradigm called “seed-and-vote". Subread is general-purpose aligner that can be used to align both genomic DNA-seq reads and RNA-seq reads. Subjunc is designed for mapping RNA-seq reads. The major differenc ...
... The align function implements the Subread aligner (Liao et al., 2013) that uses a new mapping paradigm called “seed-and-vote". Subread is general-purpose aligner that can be used to align both genomic DNA-seq reads and RNA-seq reads. Subjunc is designed for mapping RNA-seq reads. The major differenc ...
Molecular function - SGD-Wiki - Saccharomyces Genome Database
... • Full-time staff positions are needed for project stability. • Our top priority is to serve the needs of the research community (yeast and other), so communication with users is critically important. ...
... • Full-time staff positions are needed for project stability. • Our top priority is to serve the needs of the research community (yeast and other), so communication with users is critically important. ...
Package `Rsubread`
... the reads to find their mapping locations. The seed-and-vote paradigm has been found to be not only more accurate than the conventional seed-and-extend (adopted by most aligners) in read mapping, but it is a lot more efficient (Liao et al., 2013). Both Subread and Subjunc can be used to align reads ...
... the reads to find their mapping locations. The seed-and-vote paradigm has been found to be not only more accurate than the conventional seed-and-extend (adopted by most aligners) in read mapping, but it is a lot more efficient (Liao et al., 2013). Both Subread and Subjunc can be used to align reads ...
Genomic Analysis of Arabidopsis Thaliana
... use, not for plant scientists. As a result, crops have a genetic bias for agricultural purposes, such as increased yield, growth, and development. Typical crop plants have one generation a year and require large fields to grow in numbers sufficient for genetic analysis. Polyploidy and allopolyploidy ...
... use, not for plant scientists. As a result, crops have a genetic bias for agricultural purposes, such as increased yield, growth, and development. Typical crop plants have one generation a year and require large fields to grow in numbers sufficient for genetic analysis. Polyploidy and allopolyploidy ...
Abstracts - School of Life Sciences
... concerns regarding food safety and public health. Horizontal transfers by conjugation and mobilisation within this bacterial group were studied in foodstuffs. The conjugative behaviour of B. thuringiensis strains was compared in LB medium, milk and rice pudding, using three plasmids: the conjugative ...
... concerns regarding food safety and public health. Horizontal transfers by conjugation and mobilisation within this bacterial group were studied in foodstuffs. The conjugative behaviour of B. thuringiensis strains was compared in LB medium, milk and rice pudding, using three plasmids: the conjugative ...
Part 3 - Bioinformatics.ca
... • Many coding genes are well annotated with their biological functions. Non-coding regions typically lack such annotation. GREAT assigns biological meaning to a set of non-coding genomic regions by analyzing the annotations of the nearby genes. Thus, it is particularly useful in studying functions o ...
... • Many coding genes are well annotated with their biological functions. Non-coding regions typically lack such annotation. GREAT assigns biological meaning to a set of non-coding genomic regions by analyzing the annotations of the nearby genes. Thus, it is particularly useful in studying functions o ...
Identification and comparative analysis of accessory gland
... Gryllus, respectively) was sequenced from each species and aligned (Fig. 1). It is a highly repetitive sequence making accurate alignment difficult, but showing clear homology within the repetitive structure. Further, protein secondary structural predictions by PSIPRED (McGuffin et al. 2000), indica ...
... Gryllus, respectively) was sequenced from each species and aligned (Fig. 1). It is a highly repetitive sequence making accurate alignment difficult, but showing clear homology within the repetitive structure. Further, protein secondary structural predictions by PSIPRED (McGuffin et al. 2000), indica ...
D. melanogaster - GEP Community Server
... Simulate restriction digests of 21 genomes DNA fragments range from 21-33 bp in size Calculate distance between two genomes based on number of shared fragments Seetharam AS and Stuart GW. Whole genome phylogeny for 21 Drosophila species using predicted 2b-RAD fragments. PeerJ. 2013 Dec 23;1:e226. ...
... Simulate restriction digests of 21 genomes DNA fragments range from 21-33 bp in size Calculate distance between two genomes based on number of shared fragments Seetharam AS and Stuart GW. Whole genome phylogeny for 21 Drosophila species using predicted 2b-RAD fragments. PeerJ. 2013 Dec 23;1:e226. ...
Molecular cloning and evolutionary analysis of captive forest musk
... genes also form a multi-gene family and display high sequence similarity (Conte et al., 2002; Shi et al., 2003). Coding regions of T2Rs contain an average of 300 codons, and they are not interrupted by introns, rendering them easy to be detected in whole-genome sequences. Genomic analyses using comp ...
... genes also form a multi-gene family and display high sequence similarity (Conte et al., 2002; Shi et al., 2003). Coding regions of T2Rs contain an average of 300 codons, and they are not interrupted by introns, rendering them easy to be detected in whole-genome sequences. Genomic analyses using comp ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.