Magnusiomyces capitatus (de Hoog et al.) de Hoog et Smith
... 4. Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBER Safe™, and was found to be of high molecular weight. 5. No RNA was detected by electrophoresis. 6. Functional activity was confirmed by PCR amplification of approximately 1500 base pairs fragment of rRNA ...
... 4. Integrity of DNA was determined by electrophoresis on a 1% agarose gel stained with SYBER Safe™, and was found to be of high molecular weight. 5. No RNA was detected by electrophoresis. 6. Functional activity was confirmed by PCR amplification of approximately 1500 base pairs fragment of rRNA ...
Assessing the Homogeneity of Plasmid DNA: An Important
... the topology of plasmid structures. Supercoiled ccc molecules (monomers and dimers) have the most compact structure with the highest electrophoretic mobility—appearing earlier than linearized (monomers and dimers) forms that are followed by the open circular forms. This order of migration is further ...
... the topology of plasmid structures. Supercoiled ccc molecules (monomers and dimers) have the most compact structure with the highest electrophoretic mobility—appearing earlier than linearized (monomers and dimers) forms that are followed by the open circular forms. This order of migration is further ...
Scaling law characterizing the dynamics of the transition of HIV
... mutagen KP1212 has been observed to increase mutational frequencies by 40–90% (i.e., <2-fold) in the HIV-1 envelope gene in vitro (Harris et al 2005). Its pro-drug, KP1461, employed in the trial, appeared to yield a much smaller, ∼20%, increase in the so-called private mutational frequency in vivo ( ...
... mutagen KP1212 has been observed to increase mutational frequencies by 40–90% (i.e., <2-fold) in the HIV-1 envelope gene in vitro (Harris et al 2005). Its pro-drug, KP1461, employed in the trial, appeared to yield a much smaller, ∼20%, increase in the so-called private mutational frequency in vivo ( ...
Doctoral thesis (extended summary)
... other common bacterial pathogens. Results comparable to the reference method (PFGE) could be achieved within one day in a closed system and confirmed a nosocomial outbreak in Kalmar County. In Paper VI whole genome sequencing followed by bioinformatic analysis resolved transmission links within a no ...
... other common bacterial pathogens. Results comparable to the reference method (PFGE) could be achieved within one day in a closed system and confirmed a nosocomial outbreak in Kalmar County. In Paper VI whole genome sequencing followed by bioinformatic analysis resolved transmission links within a no ...
Effect of Citrus aurantifolia juice on the shelf
... particles or the environment can serve as a source of additional microbial contamination of the zobo drink (Frazier and Westhoff, 1998). The result of the treatment of zobo drink sample with different concentrations of lime juice (Table 2) reviewed that the bacterial load of the drink was reduced co ...
... particles or the environment can serve as a source of additional microbial contamination of the zobo drink (Frazier and Westhoff, 1998). The result of the treatment of zobo drink sample with different concentrations of lime juice (Table 2) reviewed that the bacterial load of the drink was reduced co ...
Detecting rare gene transfer events in bacterial populations
... mobile DNA in complex environments such as soil or the gastrointestinal tract, HGT events will initially be present at an exceedingly low frequency in the overall bacterial population. It ...
... mobile DNA in complex environments such as soil or the gastrointestinal tract, HGT events will initially be present at an exceedingly low frequency in the overall bacterial population. It ...
use of genomic tools to discover the cause of
... I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) o ...
... I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) o ...
Single-copy nuclear genes resolve the phylogeny of the
... often placed as sister to Mecopterida and the latter traditionally included either within, or as sister to, Coleoptera [9,10]. The consensus view is that most morphological features of Hymenoptera and Strepsiptera are too highly modified to unequivocally resolve their phylogenetic positions [11,12]. ...
... often placed as sister to Mecopterida and the latter traditionally included either within, or as sister to, Coleoptera [9,10]. The consensus view is that most morphological features of Hymenoptera and Strepsiptera are too highly modified to unequivocally resolve their phylogenetic positions [11,12]. ...
PDF
... site as the 5′ boundary of a gene, and there are currently no computational tools to predict the noncoding first exons or noncoding portion of a first exon. Based on our current first-exon data, approximately 40% of the human genes have completely noncoding first exons. For most of these genes, the ...
... site as the 5′ boundary of a gene, and there are currently no computational tools to predict the noncoding first exons or noncoding portion of a first exon. Based on our current first-exon data, approximately 40% of the human genes have completely noncoding first exons. For most of these genes, the ...
Discriminating Between Annual and Perennial
... 2. The Taqman Allelic Discrimination Assay will be the fastest. ...
... 2. The Taqman Allelic Discrimination Assay will be the fastest. ...
Supplementary Information (doc 132K)
... detected with low concordance rate among different platforms based on the TS samples were removed from OCD GWAS dataset. ...
... detected with low concordance rate among different platforms based on the TS samples were removed from OCD GWAS dataset. ...
Genome Evolution Due to Allopolyploidization in Wheat
... attempted to probe the bases for the wide genetic variation characterizing these species, which accounts for their great adaptability and colonizing ability. Central to our work was the investigation of how allopolyploidization alters genome structure and expression. We found in wheat that allopolyp ...
... attempted to probe the bases for the wide genetic variation characterizing these species, which accounts for their great adaptability and colonizing ability. Central to our work was the investigation of how allopolyploidization alters genome structure and expression. We found in wheat that allopolyp ...
Deconstructing the Genome: DNA at High Resolution
... bloodstream, but the amount of oxygen in the maternal circulation is less than that found in the air that enters our lungs after birth. Both embryonic and fetal hemoglobin evolved to bind oxygen more tightly than adult hemoglobin would at the lower levels of oxygen found in the embryonic and fetal e ...
... bloodstream, but the amount of oxygen in the maternal circulation is less than that found in the air that enters our lungs after birth. Both embryonic and fetal hemoglobin evolved to bind oxygen more tightly than adult hemoglobin would at the lower levels of oxygen found in the embryonic and fetal e ...
Trawling DNA Databases For Partial Matches: What Is The FBI
... and federal databases as combined in the National DNA Index System (NDIS) hold over seven million short tandem repeat (STR) profiles from convicted offenders as well as a growing number of people who were merely arrested or detained.3 When investigators recover a DNA sample from the scene of a crime ...
... and federal databases as combined in the National DNA Index System (NDIS) hold over seven million short tandem repeat (STR) profiles from convicted offenders as well as a growing number of people who were merely arrested or detained.3 When investigators recover a DNA sample from the scene of a crime ...
SALSA MLPA KIT ME003-A1 Tumor suppressor-3 - MRC
... Aberrant methylation of CpG-islands has been shown to be associated with transcriptional inactivation of tumour suppressor genes in a wide spectrum of human cancers. CpG-islands are located in or near the promoter region or other regulatory regions of approximately 50% of human genes. This ME003-A1 ...
... Aberrant methylation of CpG-islands has been shown to be associated with transcriptional inactivation of tumour suppressor genes in a wide spectrum of human cancers. CpG-islands are located in or near the promoter region or other regulatory regions of approximately 50% of human genes. This ME003-A1 ...
gene_expression
... Partitioning is a special case of fuzzy clustering, where the weights are restricted to binary values Hierarchical clustering is also “fuzzy” in some sense ...
... Partitioning is a special case of fuzzy clustering, where the weights are restricted to binary values Hierarchical clustering is also “fuzzy” in some sense ...
Bacterial
... 12 and 15 mL per Petri dish). Replace the lid immediately and tilt the plate to spread the agar. 4. Pour a second plate in the same way. Label both plates so that you know that they contain plain LB agar. Note: If you wish to pour a few more stock plates of plain LB agar for class use, you may do s ...
... 12 and 15 mL per Petri dish). Replace the lid immediately and tilt the plate to spread the agar. 4. Pour a second plate in the same way. Label both plates so that you know that they contain plain LB agar. Note: If you wish to pour a few more stock plates of plain LB agar for class use, you may do s ...
alignable - gobics.de: Department of Bioinformatics
... For each position x and each sequence Si exist an upper bound ub(x,i) and a lower bound lb(x,i) for residues y in Si that are alignable with x ...
... For each position x and each sequence Si exist an upper bound ub(x,i) and a lower bound lb(x,i) for residues y in Si that are alignable with x ...
2- pcr primer design and reaction optimisation
... a 1/256 chance of finding a given 4-base sequence. Thus, a sixteen base sequence will statistically be present only once in every 416 bases (=4 294 967 296, or 4 billion): this is about the size of the human or maize genome, and 1000x greater than the genome size of E. coli. Thus, the association of ...
... a 1/256 chance of finding a given 4-base sequence. Thus, a sixteen base sequence will statistically be present only once in every 416 bases (=4 294 967 296, or 4 billion): this is about the size of the human or maize genome, and 1000x greater than the genome size of E. coli. Thus, the association of ...
Spider Silk - Consortium for Mathematics and its Applications
... resistance to tearing, and toughness. Spider silk would be an ideal material for a large variety of medical and technical applications, and researchers are thus interested in learning the spiders’ secrets and imitating their technique.[1] This unit has a direct purpose and an indirect purpose. Its d ...
... resistance to tearing, and toughness. Spider silk would be an ideal material for a large variety of medical and technical applications, and researchers are thus interested in learning the spiders’ secrets and imitating their technique.[1] This unit has a direct purpose and an indirect purpose. Its d ...
The Mobile Genetic Element Alu in the Human Genome
... professor in the Department of Biological Sciences, Florida International University, Miami, FL 33199. Mark A. Batzer is an assistant professor in the Pathology Department and Prescott L. Deininger is a professor in the Department of Biochemistry and Molecular ...
... professor in the Department of Biological Sciences, Florida International University, Miami, FL 33199. Mark A. Batzer is an assistant professor in the Pathology Department and Prescott L. Deininger is a professor in the Department of Biochemistry and Molecular ...
Polymorphism of genes encoding PmrAB in colistin
... low frequency (www.eucast.org), most if not all E. coli pmrAB sequences deposited in databases would represent colistinsusceptible strains (with the exception of strains ZTA11/01748 and ZTA13/02182 in the present work), and thus the link found between the mutations and colistin resistance should be ...
... low frequency (www.eucast.org), most if not all E. coli pmrAB sequences deposited in databases would represent colistinsusceptible strains (with the exception of strains ZTA11/01748 and ZTA13/02182 in the present work), and thus the link found between the mutations and colistin resistance should be ...
Ensembl variation resources Open Access Database
... Background: The maturing field of genomics is rapidly increasing the number of sequenced genomes and producing more information from those previously sequenced. Much of this additional information is variation data derived from sampling multiple individuals of a given species with the goal of discov ...
... Background: The maturing field of genomics is rapidly increasing the number of sequenced genomes and producing more information from those previously sequenced. Much of this additional information is variation data derived from sampling multiple individuals of a given species with the goal of discov ...
Using an Alu Insertion Polymorphism to Study Human
... Alu is a member of the family of short interspersed elements (SINEs) and is approximately 300 nucleotides in length. Alu owes its name to a recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not prod ...
... Alu is a member of the family of short interspersed elements (SINEs) and is approximately 300 nucleotides in length. Alu owes its name to a recognition site for the endonuclease AluI in its middle. Although Alu is sometimes called a “jumping gene,” it is not properly a gene, because it does not prod ...
Applied and Environmental Microbiology
... niches, and their ability to cause serious infections in humans (1). Currently the Burkholderia genus comprises over 60 validly described species; phylogenetic trees generated from diverse gene sequence analyses (e.g., 16S rRNA) have shown divisions within the genus, and consequently it has been sep ...
... niches, and their ability to cause serious infections in humans (1). Currently the Burkholderia genus comprises over 60 validly described species; phylogenetic trees generated from diverse gene sequence analyses (e.g., 16S rRNA) have shown divisions within the genus, and consequently it has been sep ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.