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A Large Pseudoautosomal Region on the Sex Chromosomes of the
A Large Pseudoautosomal Region on the Sex Chromosomes of the

... sex chromosome divergence resulting from suppressed recombination and involves the loss of coding regions, the accumulation of repetitive regions, and structural changes such as insertions, deletions, and inversions on the sex-specific chromosome (the Y or W). Thus, degenerate sex chromosomes have d ...
brindle genetics horse
brindle genetics horse

... “brindle” by horse breeders. We propose the term “brindle 1 (BR1)” for this specific form of brindle. In some BR1 horses the stripes were also differentially pigmented. Pedigree analyses were suggestive of a monogenic X-chromosomal semi-dominant mode of inheritance. Haplotype analyses identified a 5 ...
C Anscombe 2016 PhD
C Anscombe 2016 PhD

... cause acute or invasive human illness. For the identification of such pathogens signals must be recognised above both the background host and flora. There are two primary methods to achieve this, increasing the cell number of pathogens (e.g. culture) or detection of increased pathogen signals using ...
Problems with cloning
Problems with cloning

... Cloning is making an identical genetic copy of an organism.  Has occurred for thousands of years in ...
Characterization of melanocyte stimulating hormone
Characterization of melanocyte stimulating hormone

... racial origin, two are identical (7,8), with the third (6) differing by three MSHR amino acid changes: Thr90Ser, Pro162Arg and Ala164Arg. In our initial PCR experiments on Caucasian DNA samples we found a composite of amino acids at each of these positions, with consistent observation of Ser90, Arg1 ...
Loss of the GP46/M-2 surface membrane
Loss of the GP46/M-2 surface membrane

... other species (Fig. 2B). This suggested that the GP46/M-2 gene was absent in this species. To confirm this, chromosomes were prepared from other members of the L. braziliensis complex, spanning a wide geographical range and including representative strains of all 3 major recognized species, L. guyan ...
Plasmids
Plasmids

... chromosome (which is also circular), plasmids are quite resistant to permanent denaturation. Today, most laboratories use commercial kits for plasmid isolations, because the kits are convenient and relatively inexpensive. The kits give good yields of high-quality DNA, while avoiding the need for org ...
Ex situ - PGR Forum
Ex situ - PGR Forum

... gene bank accessions should be approximately 10% of the number of herbarium specimens, any species with a lower proportion are vulnerable ...
Genome-wide RNAi Robert Barstead
Genome-wide RNAi Robert Barstead

... Compared with typical classical genetic methods, RNAi has the advantage that the sequence of the target is known, thereby connecting mutant phenotypes with known genes. Though more detailed studies depend on the recovery of stable genetic mutants, RNAi is not simply a middling substitute for proper ...
Clin Infect Dis. - Repositorio Académico UPC
Clin Infect Dis. - Repositorio Académico UPC

... results show that EPEC load was significantly higher in children with diarrhea than in asymptomatic control subjects, especially in single pathogen. Therefore, we propose that, in addition to the aforementioned factors, the bacterial load should be considered in the interpretation of illness and col ...
Genomic surveys and expression analysis of bZIP gene family in
Genomic surveys and expression analysis of bZIP gene family in

... to the castor bean genome (http://castorbean.jcvi.org/ index.php) to obtain unique reads and reads abundance using SOAP2 software (Li et al. 2009). To compare the differential expression of genes among tissues, the expression level of each gene in the different tissues was normalized to the number o ...
Trawling DNA Databases for Partial Matches: What is the FBI Afraid
Trawling DNA Databases for Partial Matches: What is the FBI Afraid

... and federal databases as combined in the National DNA Index System (NDIS) hold over seven million short tandem repeat (STR) profiles from convicted offenders as well as a growing number of people who were merely arrested or detained. 3 When investigators recover a DNA sample from the scene of a crim ...
Features of biofilms
Features of biofilms

... Heterogeneous containing microcolonies of bacterial cells encased in an EPS matrix and separated from other microcolonies by interstitial voids (water channels) Stoodley et al. (1997) defined certain criteria or characteristics that could be considered descriptive of biofilms in general, including a ...
Influenza: The Virus
Influenza: The Virus

... • Respiratory Viral Panel (Luminex assay) – Detects influenza A & B and other respiratory viruses – Detect & discriminates seasonal subtypes (H1 or H3) – TAT 36 to 48 hrs ...
Target selected insertional mutagenesis on chromosome IV of
Target selected insertional mutagenesis on chromosome IV of

... Reverse genetics using insertional mutagenesis is an efficient experimental strategy for assessing gene functions. The maize Enhancer–Inhibitor (En–I) transposable element system was used to develop an effective reverse genetics strategy in Arabidopsis based on transposons. To generate insertion mut ...
genes is conserved among species related to
genes is conserved among species related to

... possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The 5-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of t h i s slp segment. In this study the presence of S-protein and corresponding ...
Shared mutations: Common descent or common mechanism?
Shared mutations: Common descent or common mechanism?

... consequence is that the shared mutations in the 1G5 genes are due to a biological or physical mechanism. In other words, the mutations in the 1G5 gene are non-random mutations that would produce an alignment of mutations in separated species that do not reproduce together. The alignment is not due t ...
Chromosomal breakpoint positions suggest a direct role for radiation
Chromosomal breakpoint positions suggest a direct role for radiation

... properties, but was not sucient for transformation (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause pe ...
Arsenite as an Electron Donor for Anoxygenic Photosynthesis
Arsenite as an Electron Donor for Anoxygenic Photosynthesis

... reverse primer (8F, 5’-GAGTTTGATCCTGGCTCAG-3’ and 1492R, 5’-GGTTACCTTGTTACGACTT-3’). The PCR products were cloned using the Invitrogen TA-TOPO cloning kit according to the manufacturer’s instructions. Clones were screened for correctly sized inserts (~1500 bp). Sequencing was done on replicate clone ...
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)

... AmpC β-lactamases are cephalosporinases encoded on the chromosomes of many of the Enterobacteriaceae and a few other organisms, where they mediate resistance to cephalothin, cefazolin, cefoxitin, most penicillins, and β-lactamase inhibitor-β-lactam combinations. [1]. The emission of bacteria into th ...
Original articles Class I integrons in Gram-negative
Original articles Class I integrons in Gram-negative

... Horizontal gene transfer among bacteria, directed by a strong antibiotic selective pressure, has resulted in a widespread distribution of multiple antibiotic resistance genes on plasmids and transposons among many Gramnegative isolates.1 Recent studies have shown that a conserved DNA sequence, the i ...
B. Eukaryotic RNA polymerases
B. Eukaryotic RNA polymerases

... b) The coding strand sequence is given when talking about double stranded DNA (1) Same polarity as RNA (2) Easier when referring to the genetic code (3) Always given in the 5' to 3' direction 2. Template strand a) This is the strand of DNA that RNA polymerase binds to during transcription b) It is c ...
25 Microbial ecology
25 Microbial ecology

... The first bacteria, as well as all alive ones, have appeared in water. However in more later geological periods, when on a surface of globe the soil was derived, it became main habitation of microorganisms and main arena of their vital activity. The amount of bacteria in one gram of soil can be very ...
A semantic taxonomy for diversity measures
A semantic taxonomy for diversity measures

... a ‘simplex’ community representation (i.e., the species relative abundances) with the information of a Venn-complex (the pairwise species distances dij) into a single summary statistics. Here, it is worth noticing that besides simple pairwise species distances, a Venn complex may also show more comp ...
univERsity oF copEnhAGEn - Center for Protein Research
univERsity oF copEnhAGEn - Center for Protein Research

... those are not well understood. The positive effects of microbial interactions not only assist in inhibition of undesired microorganisms including spoilage micro-organisms and food-borne pathogens, but also serve in the development of desired microorganisms, including starter cultures. An interesting ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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