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Conference title
Conference title

... read Illumina data for the de novo assembly of a non-model snail species transcriptome (Radix balthica, Basommatophora, Pulmonata), and a comparison of assembler performance. BMC Genomics, 12:317. Wheat, C. W., and H. Vogel (2011) Transcriptome sequencing goals, assembly and assessment in V. Orgogoz ...
Methods to analyze RNA expression - RNA
Methods to analyze RNA expression - RNA

... Initially each library is analyzed separately except for the two files of each library if paired ends sequencing was done. 1) The short reads are aligned on the reference genome if available 2) The transcript(s) from each gene are reconstructed. At that point the analysis is done with all the librar ...
Connectivity of Earth`s largest biomes: the deep Atlantic to the
Connectivity of Earth`s largest biomes: the deep Atlantic to the

... Mullus surmuletus, Felix-Hackradt et al. 2013 ...
Centre for Cellular and Molecular Biology
Centre for Cellular and Molecular Biology

... Most often DNA testing directly detects the most common disease-causing changes in a gene, the test result is highly accurate (~98%). In other cases, an indirect method called linkage analysis is used which may produce an (3-5%) uncertainty in predicting carrier status or diagnosis due to naturally ...
Non-Mendelian Genetics Test Review
Non-Mendelian Genetics Test Review

... Parents C & D ...
Lecture 10
Lecture 10

... The DNA contents does not reflect the complexity of the organism! Related and structurally similar species may have variation in the amount of their total DNA by a factor of 100 In humans: ~5% of DNA is transcribed and 1.5% represents coding regions (exons). The rest is made of repeats with no obvio ...
Chapter 1 Art Slides
Chapter 1 Art Slides

... “killer algae” with acres of single cell ...
Microarrays = Gene Chips
Microarrays = Gene Chips

... stool Can get an answer in 6 hours The chip has almost 30,000 pieces of genetic material taken from thousands of different viruses, bacteria, fungi and parasites – represent all recognized 1,710 vertebrate viral species and 135 bacterial, 73 fungal, and 63 parasite genera. For each family or genus a ...
Molecular Markers - Personal Web Pages
Molecular Markers - Personal Web Pages

...  May be part of or closely linked to a gene that makes a protein that affects cell survival  May be part of controlling elements  May be in the larger area of ‘non-coding’ DNA  Markers have a known location  What is being marked? ...
DNA Barcoding
DNA Barcoding

... Rate of extinction has increased from about 1 species per million to 1001000 species per million Most of these species have yet to be identified ...
Track the full extent of structural variation in a genome
Track the full extent of structural variation in a genome

... genome. Single Molecule, Real-Time (SMRT®) Sequencing provides contiguous long reads, high consensus accuracy, and uniform coverage opening up access to a broad range of structural variants (SV) across a wide range of sizes, types, and GC content. -- Discover both common and rare SVs unique to an in ...
MCB5472_Lecture_2_Feb-3-14
MCB5472_Lecture_2_Feb-3-14

... Founded in 1982 at the Los Alamos National Laboratory Initially managed at Stanford in conjunction with the BIOSCI/Bionet news groups 1989-92 transition to the NCBI on the east coast One precursor was Margaret Dayhoff’s Atlas of Protein Sequence and Structure In 1987 genbank fit onto a few 360 KB fl ...
Btec Quiz 1Samples
Btec Quiz 1Samples

... D. None of the above ...
Siena Borsani - Unisi.it - Università degli Studi di Siena
Siena Borsani - Unisi.it - Università degli Studi di Siena

... - Both had taken family vacations independently to the same three-block strip of Florida beach, called Pas-Grille, without ever meeting. They both arrived in light blue Chevrolets. - Each had worked part-time in law enforcement as sheriffs. - Both smoked Salems, and both savored an occasional Miller ...
Bioinformatics Tools
Bioinformatics Tools

... • Mount, D.W. Bioinformatics: Sequence and Genome ...
The Blueprint of Life
The Blueprint of Life

... Selective breeding, cloning, Gene cascades Complete the following sentences using appropriate words or short phrases a) Name of the species studied for selective breeding ...
Genetic Engineering
Genetic Engineering

... • Used to make millions of copies of select section of DNA • When small amount of DNA are found but large amounts are needed for analysis • Semen, blood, other tissues, long-dead specimens – DNA from all can be amplified • Thermus aquaticus – hot springs bacterium • http://www.dnalc.org/ddnalc.org/r ...
MolecularBiology1APLab6
MolecularBiology1APLab6

... • Contain random DNA fragments that are collected or exchanged w/ other bacteria • Contain nonsense information • Sometimes contain useful information like antibiotic resistance ...
Lecture 6
Lecture 6

... • Statistical analysis of the rates of homologous recombination of several different genes could determine their order on a certain chromosome, and information from many such experiments could be combined to create a genetic map specifying the rough location of known genes relative to each other. • ...
statistical testing
statistical testing

... Starting in September 2009, this unit has contributed to different research projects by providing support and expertise in programming and advanced data analysis, focusing primarily on high-throughput genomics technologies including microarrays, genotyping and next-generation sequencing. The unit is ...
Ch. 13 SOL - Groupfusion.net
Ch. 13 SOL - Groupfusion.net

... human cells able to resist antibiotics human cells unable to synthesize antibodies bacterial cells able to synthesize human insulin bacterial cells unable to synthesize human insulin ...
Molecular genetics of gene expression
Molecular genetics of gene expression

... bacterial gene for resistance to a specific herbicide. The herbicide has been manufactured by the company for many years. Using a strategy similar to that used to find glyphosate resistance, where might you start to look for a bacterium resistant to that herbicide? ...
exam II study guide
exam II study guide

... 11. State the temperature range of the body, room and refrigerator. 12. Explain how temperature can be used to preserve microbes 13. Describe how biofilms form. 14. Know what quorum sensing is with regard to biofilms. 15. Explain the medical significance of biofilms in allied health settings. Micro ...
Classification of DNA sequences using Bloom Filters
Classification of DNA sequences using Bloom Filters

... whether an element is part of a reference set • m bit vector with k hash functions • Never returns a false negative; may however return a false positive • Optimal number of hash functions; ...
Evolution and Diversity: Sometimes, differences between organisms
Evolution and Diversity: Sometimes, differences between organisms

... kind provide advantages for surviving and reproducing in different environments. These selective differences may lead to dramatic changes in characteristics of organisms in a population over extremely long periods of time. KEY IDEA: The diversity and changing of life forms over many generations is t ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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