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Age Brauer Journal Club 18th of March 2015
Age Brauer Journal Club 18th of March 2015

... •  There  is  no  “core”  resistome  –  no  single  Resfams   family  that  was  shared  across  all  samples.     •  Only  two  families  were  shared  across  >50%  of   metagenomic  samples  (class  A  beta-­‐lactamases   and  MFS  A ...
Career Advancement Workshop
Career Advancement Workshop

... Iowa State University ...
DNA RNA and Protein Synthesis with Answers
DNA RNA and Protein Synthesis with Answers

... 3. Part of a molecule found in cells is represented below ...
Supplementary Table S1
Supplementary Table S1

... ...
group3_slides
group3_slides

... ◦ The Microbiome = microbes, their genomes, and the habitat they are found ◦ The gut microbiome: ◦ The most diverse (over 100 trillion bacteria) ◦ Implicated in a range of metabolic processes ...
Molecular Microbial Ecology Symbiosis
Molecular Microbial Ecology Symbiosis

... Millions of microbial species on Earth sustain nature’s element cycles or interact in (mostly) beneficial ways with macroorganisms; only few of these microbes are known to date. We use molecular methods to study the identity, function, and regulating factors of microbes in nature, with a special foc ...
Genome Sequencing Using a Mapping Approach
Genome Sequencing Using a Mapping Approach

... Mapping Approach Ultimately through the use of these techniques a high density physical map of sequence polymorphisms can be generated and used as the basis for squencing the genome. ...
Analysis of Genomes
Analysis of Genomes

... 3. many bacteria 4. many complete nuclear genomes have been sequenced ...
messenger RNA (mRNA)
messenger RNA (mRNA)

... ...
Chapter 2 PowerPoint Slides
Chapter 2 PowerPoint Slides

... • BAC's are typically cleaved into smaller fragments -- about 2000 bases, and stored on E. coli viruses (a plasmid) • precise order of larger BACs is determined - because determining the order of many smaller fragments is more work -- however • shorter fragments are more amenable to the chemistry of ...
Genomes and sequence alignment
Genomes and sequence alignment

... Can still contain substantial contamination and error, but very usable if you don't trust specifics Miscellany GenBank accepts any identified sequence: Plasmids, chromosomes, individual transcripts, different clones/inserts, metagenomes, etc. I've never found most of these random sequences to be par ...
Bioinformatics - University of Maine System
Bioinformatics - University of Maine System

... 1. The species occurrence data is gathered from a number of biological collections housed at several museums and herbaria worldwide. Those institutions have their specimen databases linked and integrated through The Species Analyst project. ...
Microbes from a Neanderthal Bone
Microbes from a Neanderthal Bone

... that only a small part of it was actually Neanderthal, and more than twice as much came from bacteria. Moreover nothing could be said about the vast majority (80%) of the sequences, because they were not similar to anything known. Bacteria in Neanderthal genome? Where did they come from? What kind o ...
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Genetic modification and biotechnology

... that gene is present • Ex. Sickle cell gene ...
open as PDF
open as PDF

... regularly being discovered through metagenomics. We are beginning to realize the vast diversity of archaeal ecological adaptations, and how much we do not know regarding their metabolism. Though Archaea encompass extremophiles, metagenomics has shown that they are ubiquitous, documenting the diversi ...
How DNA Controls the Workings of the Cell
How DNA Controls the Workings of the Cell

... How DNA Controls the Workings of the Cell Below are two partial sequences of DNA bases (shown for only one strand of DNA) Sequence 1 is from a human and sequence 2 is from a cow. In both humans and cows, this sequence is part of a set of instructions for controlling a bodily function. In this case, ...
Bioinformatics
Bioinformatics

... application of genomics to pharmacology ...
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Genomics and Bioinformatics KEY CONCEPT Entire genomes are

... – Study of entire genomes – can include the sequencing of the genome – Compare genomes within & across species to find similarities & differences among different organisms ...
Presentation title: Introduction to RNA
Presentation title: Introduction to RNA

... comparing the quantities of each gene’s RNA between different tissue types, through development, in disease or  in different environments – known as differential gene expression analysis.   ...
BI0034
BI0034

... microarray data analysis1) Definition of a key gene and import / retrieval of the key gene sequence, 2) BLAST search of the key gene sequence against the local database, 3) Determination of the k neighboring genes at each side of the key gene, 4) All against all BLAST searches of the k neighboring g ...
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Lecture 4

... • Also, by comparing multiple species we can make inferences about sets of species. • How do we compare DNA or protein sequences of two different species? ...
Introduction to bioinformatics
Introduction to bioinformatics

... Comparing different complete genomes to infer evolutionary history and genome ...
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No Slide Title

... – several entire genomes (eg., human, mosquito, and malaria parasite) ...
Human Genome Project
Human Genome Project

... Using PCR it is then possible to determine whether your STS is present in any other clone or cell line. Obtaining STS: sequencing the ends of large cloned DNAs (BACs or YACs, for example). Uniqueness: use the cloned DNA from the STS as a probe on a Southern blot of genomic DNA: if the STS is unique, ...
Workshop-2010 - An-Najah Blogs - An
Workshop-2010 - An-Najah Blogs - An

... • Genotypic characterization using the allelic mismatches of housekeeping genes (internal fragments ~ 450 bp) • Allows definition of strains within named species (typing at intraspecis level) • Population and molecular epidemiological studies ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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