Conserved syntenic clusters of protein coding genes are missing in
... zebra finch, since these represented the best assembled and curated avian genomes available in Ensembl at the time we began this study. Out of 18,596 protein coding genes in lizard, 12,113 are predicted to have 1-to-1 orthologs in humans. Of these, only 10,554 also have 1to-1 orthologs in chicken an ...
... zebra finch, since these represented the best assembled and curated avian genomes available in Ensembl at the time we began this study. Out of 18,596 protein coding genes in lizard, 12,113 are predicted to have 1-to-1 orthologs in humans. Of these, only 10,554 also have 1to-1 orthologs in chicken an ...
PCR Clean-up Kit / 96-well PCR Clean
... is ideal for use on automated sample preparation systems as a method for the high-throughput purification of plasmid intended for sequence analysis on platforms such as the ABI PRISM® 3700 or 3730 DNA Analyzers. This kit is not recommended for the purification of large recombinant constructs, such a ...
... is ideal for use on automated sample preparation systems as a method for the high-throughput purification of plasmid intended for sequence analysis on platforms such as the ABI PRISM® 3700 or 3730 DNA Analyzers. This kit is not recommended for the purification of large recombinant constructs, such a ...
HW4_final
... Transcription Factors (which usually bind double-stranded DNA) and compete with the natural promoter of a gene which is regulated by that Transcription Factor. This is another elegant way by which the cell can regulate the gene expression. To-date there are only very few known examples of lncRNA tha ...
... Transcription Factors (which usually bind double-stranded DNA) and compete with the natural promoter of a gene which is regulated by that Transcription Factor. This is another elegant way by which the cell can regulate the gene expression. To-date there are only very few known examples of lncRNA tha ...
Conserved syntenic clusters of protein coding genes are missing in birds
... analyzed (galGal4; 2011) is currently the best-assembled and most completely sequenced avian genome, with much shorter and fewer gaps and thus more complete than the version described in Hillier et al. [5]. Accordingly, this latest assembly contains the orthologs for many conserved genes that could ...
... analyzed (galGal4; 2011) is currently the best-assembled and most completely sequenced avian genome, with much shorter and fewer gaps and thus more complete than the version described in Hillier et al. [5]. Accordingly, this latest assembly contains the orthologs for many conserved genes that could ...
Antimicrobial Prospective of Parmotrema perlatum Hexane Extract
... have led to emergence of Multi Drug Resistance (MDR) in these microorganisms. In 2012, there were about 4,50,000 new cases of MDR tuberculosis. Resistance to earlier generation anti malarial drug is reported in most malarial endemic countries. MDR Klebsiella and E. coli have been isolated in hospita ...
... have led to emergence of Multi Drug Resistance (MDR) in these microorganisms. In 2012, there were about 4,50,000 new cases of MDR tuberculosis. Resistance to earlier generation anti malarial drug is reported in most malarial endemic countries. MDR Klebsiella and E. coli have been isolated in hospita ...
Identification of novel endogenous antisense transcripts by DNA
... conserved NATs (antisense of Acaa1b and Aard) by performing Northern and in situ hybridization (ISH) analyses. Whereas human ACAA1 (acetyl-Coenzyme A acyltransferase 1) overlaps with DLEC1 (deleted in lung and esophageal cancer 1) in a tail-to-tail overlapping manner, its orthologous counterpart in ...
... conserved NATs (antisense of Acaa1b and Aard) by performing Northern and in situ hybridization (ISH) analyses. Whereas human ACAA1 (acetyl-Coenzyme A acyltransferase 1) overlaps with DLEC1 (deleted in lung and esophageal cancer 1) in a tail-to-tail overlapping manner, its orthologous counterpart in ...
Lactococcus lactis LM0230 contains a single
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
Distribution of orphan metabolic activities
... combined computational–experimental effort. Several computational and experimental approaches can help to accelerate the identification of genes for metabolic orphans. Although homology-based computational methods [37–40] will not be accurate without reference sequence information, genomic context c ...
... combined computational–experimental effort. Several computational and experimental approaches can help to accelerate the identification of genes for metabolic orphans. Although homology-based computational methods [37–40] will not be accurate without reference sequence information, genomic context c ...
detection, recovery, isolation and characterization
... Abstract.............................................ii ...
... Abstract.............................................ii ...
Chapter 1: Microbial contamination in space
... Bacterial isolates, strains and growth conditions ........................................ 43 DNA extraction .......................................................................................... 45 Phylogenetic analysis ........................................................................... ...
... Bacterial isolates, strains and growth conditions ........................................ 43 DNA extraction .......................................................................................... 45 Phylogenetic analysis ........................................................................... ...
Genetic diversity and epidemiology of infectious hematopoietic
... 6 or 7 d post-inoculation. Infected cells and media for each virus isolate were centrifuged and 1.0 m1 aliquots of supernatant were frozen at -7O0C. These served as the virus stock used in the study. The reference virus strain, RB1, isolated in 1975 from steelhead Oncorhynchus mykiss at the Round Bu ...
... 6 or 7 d post-inoculation. Infected cells and media for each virus isolate were centrifuged and 1.0 m1 aliquots of supernatant were frozen at -7O0C. These served as the virus stock used in the study. The reference virus strain, RB1, isolated in 1975 from steelhead Oncorhynchus mykiss at the Round Bu ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... patient and a potential donor have the same HLA type, the transplant has a good chance of being successful. If a patient and a donor are HLA mismatched, the donor’s stem cells may be destroyed by the patient's immune system (graft rejection) or the immune system cells in the donor’s stem cell prepar ...
... patient and a potential donor have the same HLA type, the transplant has a good chance of being successful. If a patient and a donor are HLA mismatched, the donor’s stem cells may be destroyed by the patient's immune system (graft rejection) or the immune system cells in the donor’s stem cell prepar ...
The Mycobacterium tuberculosis katG promoter region contains a
... Bashyam et al., 1996) ; however, many of these promoters are specifically regulated in vivo and may not be typical. Some of the mycobacterial promoters resemble the typical Escherichia coli σ(! consensus promoter and function in this organism, but most have a higher GjC content and differ from the E ...
... Bashyam et al., 1996) ; however, many of these promoters are specifically regulated in vivo and may not be typical. Some of the mycobacterial promoters resemble the typical Escherichia coli σ(! consensus promoter and function in this organism, but most have a higher GjC content and differ from the E ...
- Clinical Microbiology and Infection
... taxonomically relevant subspecies [15–18]. At the same time, however, there are inherently polymorphic loci present in the genomes of all bacterial species that enable further subspecies differentiation. Thus, DNA typing, which essentially comprises the direct or indirect assessment of subspecies nu ...
... taxonomically relevant subspecies [15–18]. At the same time, however, there are inherently polymorphic loci present in the genomes of all bacterial species that enable further subspecies differentiation. Thus, DNA typing, which essentially comprises the direct or indirect assessment of subspecies nu ...
extremely thermophilic microorganisms and their polymer
... to their metabolism, since they include methanogens, sulphate-reducers, nitrate-reducers and also the aerobic respirers. However the majority of the species know at the present are strictly anaerobic heterotrophic S0 reducers (24). Among the terrestrial Archaea, three groups can be distinguished. Ac ...
... to their metabolism, since they include methanogens, sulphate-reducers, nitrate-reducers and also the aerobic respirers. However the majority of the species know at the present are strictly anaerobic heterotrophic S0 reducers (24). Among the terrestrial Archaea, three groups can be distinguished. Ac ...
DNA
... • 1950s – Mahlon Bush Hoagland first to isolate tRNA • 1952 – Alfred Hershey and Martha Chase make genes from DNA ...
... • 1950s – Mahlon Bush Hoagland first to isolate tRNA • 1952 – Alfred Hershey and Martha Chase make genes from DNA ...
Persistence and dispersion of Acinetobacter spp. in the urban water
... groundwater supplies. Besides, Acinetobacter spp. are normal inhabitants of the skin in healthy people (Bergogne-Berezin & Towner, 1996). Given this ubiquity, Acinetobacter spp. are considered opportunistic pathogens with potential public health significance. In the last three decades, Acinetobacter ...
... groundwater supplies. Besides, Acinetobacter spp. are normal inhabitants of the skin in healthy people (Bergogne-Berezin & Towner, 1996). Given this ubiquity, Acinetobacter spp. are considered opportunistic pathogens with potential public health significance. In the last three decades, Acinetobacter ...
Acinetobacter baumannii
... (Table 2.2). Four non-Australian A. baumannii strains were also included in the characterisation; AB0057 (Adams et al. 2008), AYE (Fournier et al. 2006), ATCC 19606 and ATCC 17978 (Smith et al. 2007). The site of isolation of the strains has been listed in Table 2.2. The clonal groupings amongst cli ...
... (Table 2.2). Four non-Australian A. baumannii strains were also included in the characterisation; AB0057 (Adams et al. 2008), AYE (Fournier et al. 2006), ATCC 19606 and ATCC 17978 (Smith et al. 2007). The site of isolation of the strains has been listed in Table 2.2. The clonal groupings amongst cli ...
Title of SMI goes here - Public Health England
... (MALDI-TOF MS), is increasingly being applied for organism identification within diagnostic microbiology laboratories. Identification using this technology affords the opportunity to rapidly and cost-effectively identify bacteria, most yeast species as well as some genera of filamentous fungi in com ...
... (MALDI-TOF MS), is increasingly being applied for organism identification within diagnostic microbiology laboratories. Identification using this technology affords the opportunity to rapidly and cost-effectively identify bacteria, most yeast species as well as some genera of filamentous fungi in com ...
Semantic resources for biomedical data integration: phenotypes
... The biomedical knowledge domain provides lot of ontologies and electronic data bases. ...
... The biomedical knowledge domain provides lot of ontologies and electronic data bases. ...
characterizing the genetic bases of autosomal recessive disorders
... and their families. Elucidating the genetic bases of such disorders is essential to improve their clinical outcome and for implementing effective prevention programs. In this dissertation, the genetic bases of seven autosomal recessive disorders in consanguineous families were investigated using hom ...
... and their families. Elucidating the genetic bases of such disorders is essential to improve their clinical outcome and for implementing effective prevention programs. In this dissertation, the genetic bases of seven autosomal recessive disorders in consanguineous families were investigated using hom ...
Rapid identification of Acinetobacter baumannii, Acinetobacter
... and Acinetobacter johnsonii) were found. The identification of all species was confirmed by ITS sequence analysis. PFGE results showed that the clinical Acb complex isolates in the first and second sets had diverse pulsotypes (data not shown). At present, PCR-based methods to identify A. baumannii u ...
... and Acinetobacter johnsonii) were found. The identification of all species was confirmed by ITS sequence analysis. PFGE results showed that the clinical Acb complex isolates in the first and second sets had diverse pulsotypes (data not shown). At present, PCR-based methods to identify A. baumannii u ...
Population Structure and Functional Analyses, by In
... inconspicuous. As a consequence, additional properties like growth with different carbon and energy sources, base composition of the DNA, and cell wall components have been catalogized (in Bergey's Manual of Systematic Bacteriology, Murray et al., 1984 and The Prokaryotes, Balows et al., 1992) and a ...
... inconspicuous. As a consequence, additional properties like growth with different carbon and energy sources, base composition of the DNA, and cell wall components have been catalogized (in Bergey's Manual of Systematic Bacteriology, Murray et al., 1984 and The Prokaryotes, Balows et al., 1992) and a ...
Genotype C of hepatitis B virus can be classified into at least two
... the databases over the pre-S region. Forty-three isolates with full genome sequences (10 in this study and 33 from database entries) were also analysed. Nucleotide sequences were multiple-aligned using GENETYX for Windows version 6.0 software (Genetyx) and corrected manually by visual inspection. Ge ...
... the databases over the pre-S region. Forty-three isolates with full genome sequences (10 in this study and 33 from database entries) were also analysed. Nucleotide sequences were multiple-aligned using GENETYX for Windows version 6.0 software (Genetyx) and corrected manually by visual inspection. Ge ...
PDF Links - The Korean Journal of Parasitology
... amoeba host were incubated on Mycobacterium specific media, Middlebrook 7H11 agar. The trophozoites of Acanthamoeba KA/LC6 (approximately 1 × 105) were washed 3 times with PBS and sonicated for 0, 1, 5, 10, 30, 60, or 90 sec. The sonicated cell suspensions were inoculated on Middlebrook 7H11 agar an ...
... amoeba host were incubated on Mycobacterium specific media, Middlebrook 7H11 agar. The trophozoites of Acanthamoeba KA/LC6 (approximately 1 × 105) were washed 3 times with PBS and sonicated for 0, 1, 5, 10, 30, 60, or 90 sec. The sonicated cell suspensions were inoculated on Middlebrook 7H11 agar an ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.