PDF Links - The Korean Journal of Parasitology
... amoeba host were incubated on Mycobacterium specific media, Middlebrook 7H11 agar. The trophozoites of Acanthamoeba KA/LC6 (approximately 1 × 105) were washed 3 times with PBS and sonicated for 0, 1, 5, 10, 30, 60, or 90 sec. The sonicated cell suspensions were inoculated on Middlebrook 7H11 agar an ...
... amoeba host were incubated on Mycobacterium specific media, Middlebrook 7H11 agar. The trophozoites of Acanthamoeba KA/LC6 (approximately 1 × 105) were washed 3 times with PBS and sonicated for 0, 1, 5, 10, 30, 60, or 90 sec. The sonicated cell suspensions were inoculated on Middlebrook 7H11 agar an ...
Probable presence of an ubiquitous cryptic mitochondrial gene on
... potentially functional gau regions have been found in nuclear genomes. However, a recent bioinformatics study showed that several hypothetical overlapping mt genes could be predicted, including gau; this involves the possible import of the cytosolic AGR tRNA into the mitochondria and/or the expressi ...
... potentially functional gau regions have been found in nuclear genomes. However, a recent bioinformatics study showed that several hypothetical overlapping mt genes could be predicted, including gau; this involves the possible import of the cytosolic AGR tRNA into the mitochondria and/or the expressi ...
CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI The
... Most cancer genomes are extensively altered by point mutations and chromosome rearrangements. Although mutations are generally thought to accumulate gradually, over many cell division cycles1,2, recent cancer genome sequencing provides evidence for mutational processes that generate multiple mutatio ...
... Most cancer genomes are extensively altered by point mutations and chromosome rearrangements. Although mutations are generally thought to accumulate gradually, over many cell division cycles1,2, recent cancer genome sequencing provides evidence for mutational processes that generate multiple mutatio ...
Evolutionary relationships between the former species
... strains. In the case of S. pastorianus strains, SuGDH1* was found in strain NRRL Y-1551; or with one substitution: in strain CBS 1513 (S. carlsbergensis), T306 replaced C (silent), while in strains CBS 1538NT and CBS 1503 (S. monacensis), G89 was replaced by A, resulting in a R(AGA) to K(AAA) change ...
... strains. In the case of S. pastorianus strains, SuGDH1* was found in strain NRRL Y-1551; or with one substitution: in strain CBS 1513 (S. carlsbergensis), T306 replaced C (silent), while in strains CBS 1538NT and CBS 1503 (S. monacensis), G89 was replaced by A, resulting in a R(AGA) to K(AAA) change ...
PDF
... by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but their function and target genes in unicellular branches of the green lineage remain ...
... by catalyzing H3K27 methylation and H2AK118/119 mono-ubiquitination through the activity of the Polycomb repressive complex 2 (PRC2) and PRC1, respectively. PcG proteins were extensively studied in higher plants, but their function and target genes in unicellular branches of the green lineage remain ...
Control of ribosome traffic by position-dependent
... stop-codon reflect the ribosome diameter (11 codons). When one C-rate codon is added at the beginning (Fig. 3b), it increases the coverage of the start codon to somewhat. This slightly reduces the initiation rate resulting in lower coverage and fewer collisions between ribosomes. As the Crate codon ...
... stop-codon reflect the ribosome diameter (11 codons). When one C-rate codon is added at the beginning (Fig. 3b), it increases the coverage of the start codon to somewhat. This slightly reduces the initiation rate resulting in lower coverage and fewer collisions between ribosomes. As the Crate codon ...
Applied Microbiology and Biotechnology
... SDS PAGE was subjected to MALDI MS. Since this peptide mass fingerprinting revealed that no protein homologous to ASR1 phytase has been deposited within the protein databases, the highly sensitive nanoflowelectrospray mass spectrometry technique was applied in order to obtain specific sequence infor ...
... SDS PAGE was subjected to MALDI MS. Since this peptide mass fingerprinting revealed that no protein homologous to ASR1 phytase has been deposited within the protein databases, the highly sensitive nanoflowelectrospray mass spectrometry technique was applied in order to obtain specific sequence infor ...
Text S1.
... [2] or the 1000 Genomes Project [3]). Putatively functional genotypes are only counted if they occur in genes annotated as being associated with the phenotype. Second layer. These nodes represent genes, split into those annotated as high penetrance GH or low penetrance GL. Their values depend on lin ...
... [2] or the 1000 Genomes Project [3]). Putatively functional genotypes are only counted if they occur in genes annotated as being associated with the phenotype. Second layer. These nodes represent genes, split into those annotated as high penetrance GH or low penetrance GL. Their values depend on lin ...
Concordance trees, concordance factors, and the exploration of
... data for a number of loci for a set of individuals representative of the taxa under study. If we are prepared to make some assumptions about the nature of populations and genetic processes, these data contain information on the history of the populations from which they are drawn. Therefore, the ide ...
... data for a number of loci for a set of individuals representative of the taxa under study. If we are prepared to make some assumptions about the nature of populations and genetic processes, these data contain information on the history of the populations from which they are drawn. Therefore, the ide ...
Selecting Degenerate Multiplex PCR Primers
... Single Nucleotide Polymorphisms (SNPs) are individual base differences in DNA sequences between individuals. It is estimated that there are roughly three million SNPs in the human genome [12]. Association studies between SNPs and various diseases, as well as differences in how individuals respond to ...
... Single Nucleotide Polymorphisms (SNPs) are individual base differences in DNA sequences between individuals. It is estimated that there are roughly three million SNPs in the human genome [12]. Association studies between SNPs and various diseases, as well as differences in how individuals respond to ...
Bacterial canker and blast of stone fruit trees, caused by
... 1- Pectate lyase (PL) is an important tissue-macerating enzyme, which can be produced by a wide variety of phytopathogenic bacteria, especially those capable of causing soft rot, such as Erwinia and Pseudomonas. The PL produced by softrotting P. viridiflava and P. fluorescens is usually found as a s ...
... 1- Pectate lyase (PL) is an important tissue-macerating enzyme, which can be produced by a wide variety of phytopathogenic bacteria, especially those capable of causing soft rot, such as Erwinia and Pseudomonas. The PL produced by softrotting P. viridiflava and P. fluorescens is usually found as a s ...
Intellectual property rights and innovation: Evidence from
... the expression of a gene into a trait such as the presence or absence of a disease. For each gene, I collect data on publications investigating potential genotype-phenotype links, on successfully generated scientific knowledge about genotype-phenotype links, and on the development of genebased diagn ...
... the expression of a gene into a trait such as the presence or absence of a disease. For each gene, I collect data on publications investigating potential genotype-phenotype links, on successfully generated scientific knowledge about genotype-phenotype links, and on the development of genebased diagn ...
Molecular Phylogeny of Symbiotic
... over time and space. At the present time, we have found only one type of symbiont present in a host. There was no evidence for different species of dinoflagellate symbionts within either the foraminifera or the radiolaria over three different collecting trips (preliminary data from third trip not sh ...
... over time and space. At the present time, we have found only one type of symbiont present in a host. There was no evidence for different species of dinoflagellate symbionts within either the foraminifera or the radiolaria over three different collecting trips (preliminary data from third trip not sh ...
Genomic organisation of the Mal d 1 gene cluster on linkage group
... in Mal d 1 isoallergens that are not expressed in fruit and, thus, are not involved in allergic reactions. The observed functional differentiation among isoallergens and variants suggests the need for in-depth knowledge about the existence and performance of individual proteins and, thus, of individ ...
... in Mal d 1 isoallergens that are not expressed in fruit and, thus, are not involved in allergic reactions. The observed functional differentiation among isoallergens and variants suggests the need for in-depth knowledge about the existence and performance of individual proteins and, thus, of individ ...
Contribution of Horizontal Gene Transfer to Virulence
... Co-chairs: Harry L.T. Mobley and Betsy Foxman ...
... Co-chairs: Harry L.T. Mobley and Betsy Foxman ...
- University of Mississippi
... units was determined, and representative cultures from each sample were identified using 16S rRNA gene sequencing. While more bacteria were found in chocolate ice cream than strawberry or vanilla, high variability in bacterial counts between brands meant that differences between flavors were not sta ...
... units was determined, and representative cultures from each sample were identified using 16S rRNA gene sequencing. While more bacteria were found in chocolate ice cream than strawberry or vanilla, high variability in bacterial counts between brands meant that differences between flavors were not sta ...
Electrophoresis Revised
... placed firmly on the end. Since the principle by which the micropipette works is the creation of a vacuum in the tip, causing liquid to be drawn up, it is critical that the tip be on tight enough to make an air-tight seal. Having said this, do NOT jam the tips on so hard that they are hard to get of ...
... placed firmly on the end. Since the principle by which the micropipette works is the creation of a vacuum in the tip, causing liquid to be drawn up, it is critical that the tip be on tight enough to make an air-tight seal. Having said this, do NOT jam the tips on so hard that they are hard to get of ...
Inheritance of White Colour in Alpacas
... at the time of the Spanish conquest, and to subsequent lack of research into the field. In very recent times, molecular genetics tools have been made available to the alpaca research community that allow us to study pigmentation in alpacas much more easily and thoroughly. These tools include the alp ...
... at the time of the Spanish conquest, and to subsequent lack of research into the field. In very recent times, molecular genetics tools have been made available to the alpaca research community that allow us to study pigmentation in alpacas much more easily and thoroughly. These tools include the alp ...
ARTICLES - Weizmann Institute of Science
... Indeed, several types of regions had markedly high or low predicted occupancy. The highest predicted occupancy was over centromeres, indicating that centromere function requires enhanced stability of histone–DNA interactions that are encoded in the genomic sequence. One might think that genomes woul ...
... Indeed, several types of regions had markedly high or low predicted occupancy. The highest predicted occupancy was over centromeres, indicating that centromere function requires enhanced stability of histone–DNA interactions that are encoded in the genomic sequence. One might think that genomes woul ...
DNA breathing dynamics distinguish binding from nonbinding
... LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardi ...
... LMD simulations distinguish true YY1 binding from nonbinding sites in the human PLG promoter YY1 knockdown in HeLa cells coincides with the accumulation of plasminogen (PLG) mRNA (not shown). The gene product regulates a wide variety of biologic responses directly related to the development of cardi ...
Multiplex PCR for specific identification and determination of mating
... (DNASTAR) and a consensus sequence was computed from the forward and reverse sequence. A megablast nucleotide search analysis was carried out at NCBI’s GenBank nucleotide database and sequences with high similarity were downloaded from GenBank and included in alignment files. The sequences were alig ...
... (DNASTAR) and a consensus sequence was computed from the forward and reverse sequence. A megablast nucleotide search analysis was carried out at NCBI’s GenBank nucleotide database and sequences with high similarity were downloaded from GenBank and included in alignment files. The sequences were alig ...
Problems caused by biofilms
... will rapidly grow to large numbers. Bouman et al. (1982) found up to 106 bacteria per cm2 in regenerative sections of pasteurisers after 12 hours of operation. This will cause contamination for example with Streptococcus thermophilus, which is reported to reach a contamination level of about 107 ce ...
... will rapidly grow to large numbers. Bouman et al. (1982) found up to 106 bacteria per cm2 in regenerative sections of pasteurisers after 12 hours of operation. This will cause contamination for example with Streptococcus thermophilus, which is reported to reach a contamination level of about 107 ce ...
Release factor 2 frameshifting sites in different bacteria
... sequences from the 86 additional bacterial species now available have been analyzed. Thirty percent of them have a single ORF and their expression does not require frameshifting. In the ~70% that utilize frameshifting, the sequence cassette responsible for frameshifting is highly conserved. In the E ...
... sequences from the 86 additional bacterial species now available have been analyzed. Thirty percent of them have a single ORF and their expression does not require frameshifting. In the ~70% that utilize frameshifting, the sequence cassette responsible for frameshifting is highly conserved. In the E ...
A genome-wide analysis of DNA methylation in buccal - VU-DARE
... average genome-wide heritability of DNA methylation was higher when restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher a ...
... average genome-wide heritability of DNA methylation was higher when restricting to the most variable CpG sites (for the top 10% CpGs of which methylation level varied most between subjects, the average heritability was 37%) 34. It was also found that gene body and intergenic regions showed higher a ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.