The Nicotiana tabacum genome encodes two cytoplasmic
... and Luk 1988;Tonissen et al. 1989; Wollman et al. 1988; Tonissen and Wells 1991), algae (Gleason et al. 1985; Muller and Buchanan 1989; Decottignies et al. 1990, 1991) and fungi (Gan 1991; Muller 1991; Wetterauer et al. 1992). Apart from the active dithiol centre which is common to all thioredoxins ...
... and Luk 1988;Tonissen et al. 1989; Wollman et al. 1988; Tonissen and Wells 1991), algae (Gleason et al. 1985; Muller and Buchanan 1989; Decottignies et al. 1990, 1991) and fungi (Gan 1991; Muller 1991; Wetterauer et al. 1992). Apart from the active dithiol centre which is common to all thioredoxins ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
... of our new pRS plasmids carrying MX4 drug resistance cassettes in PCR-mediated gene replacement (File S1). Yeast cultures were grown in standard YEPD medium (1% yeast extract, 2% peptone, 0.012% adenine, 0.006% uracil, and 2% dextrose), except during selection; plate media were prepared by adding 2% ...
... of our new pRS plasmids carrying MX4 drug resistance cassettes in PCR-mediated gene replacement (File S1). Yeast cultures were grown in standard YEPD medium (1% yeast extract, 2% peptone, 0.012% adenine, 0.006% uracil, and 2% dextrose), except during selection; plate media were prepared by adding 2% ...
Counterinsurgency Doctrine Applied to Infectious Disease
... kills all bacteria will also kill the patient. First, some antibiotics do not penetrate the patient. They are useful for skin or gastrointestinal infections, but such antibiotics cannot reach infections that are internal or disseminated and, therefore, have limited clinical application. Generally, t ...
... kills all bacteria will also kill the patient. First, some antibiotics do not penetrate the patient. They are useful for skin or gastrointestinal infections, but such antibiotics cannot reach infections that are internal or disseminated and, therefore, have limited clinical application. Generally, t ...
Detecting copy number variants and runs of homozygosity on a
... invaluable insight into a number of conditions. When analysed in conjunction with copy number variation (CNV) data from array comparative genomic hybridisation (aCGH) arrays, this insight can aid in the identification of additional genetic variants to those yielded by the CNV data alone. Protocols f ...
... invaluable insight into a number of conditions. When analysed in conjunction with copy number variation (CNV) data from array comparative genomic hybridisation (aCGH) arrays, this insight can aid in the identification of additional genetic variants to those yielded by the CNV data alone. Protocols f ...
This document has been downloaded from Tampub – The
... Protocol 1962 [24] and the WHO Study Group in Europe [25]. The areas of coronary artery wall covered by fatty streaks as well as fibrous and complicated lesions were measured with a computer-assisted planimetric technique. The details have been described in a previous publication [26]. The areas of ...
... Protocol 1962 [24] and the WHO Study Group in Europe [25]. The areas of coronary artery wall covered by fatty streaks as well as fibrous and complicated lesions were measured with a computer-assisted planimetric technique. The details have been described in a previous publication [26]. The areas of ...
Bacterial and fungal infections
... fastidious or slow-growing microorganisms. Major evolutions have been made so far, but even today, the ideal technique, in which simultaneous pathogen identification and determination of the antimicrobial susceptibility pattern is provided, does not exist. Throughout the years, many technologies wer ...
... fastidious or slow-growing microorganisms. Major evolutions have been made so far, but even today, the ideal technique, in which simultaneous pathogen identification and determination of the antimicrobial susceptibility pattern is provided, does not exist. Throughout the years, many technologies wer ...
Novel pathogen-specific primers for the detection of Agrobacterium
... crown gall (BURR et al. 1998). Besides A. vitis, Agrobacterium tumefaciens may also occur on grapevines as causative agent of crown gall disease (SZEGEDI et al. 2005). The polymerase chain reaction (PCR) has widely been used to test for the presence of various plant pathogens to select clean plant m ...
... crown gall (BURR et al. 1998). Besides A. vitis, Agrobacterium tumefaciens may also occur on grapevines as causative agent of crown gall disease (SZEGEDI et al. 2005). The polymerase chain reaction (PCR) has widely been used to test for the presence of various plant pathogens to select clean plant m ...
Convergent Evolution in the Genetic Basis of Müllerian
... occur during spermatogenesis in Lepidoptera). Direct estimation of recombination between HmB and HmD was not possible as these dominant cosegregating phenotypes are inherited in repulsion (Figure 2). Linkage map assembly: The H. erato linkage map was adapted from Kapan et al. (2006). MapMaker Macint ...
... occur during spermatogenesis in Lepidoptera). Direct estimation of recombination between HmB and HmD was not possible as these dominant cosegregating phenotypes are inherited in repulsion (Figure 2). Linkage map assembly: The H. erato linkage map was adapted from Kapan et al. (2006). MapMaker Macint ...
Convergent Evolution in the Genetic Basis of Müllerian Mimicry in
... occur during spermatogenesis in Lepidoptera). Direct estimation of recombination between HmB and HmD was not possible as these dominant cosegregating phenotypes are inherited in repulsion (Figure 2). Linkage map assembly: The H. erato linkage map was adapted from Kapan et al. (2006). MapMaker Macint ...
... occur during spermatogenesis in Lepidoptera). Direct estimation of recombination between HmB and HmD was not possible as these dominant cosegregating phenotypes are inherited in repulsion (Figure 2). Linkage map assembly: The H. erato linkage map was adapted from Kapan et al. (2006). MapMaker Macint ...
A Genome-Wide Survey of the NAC Transcription
... Transcription factors are proteins that modulate gene expression by binding to specific cis-acting promoter elements, thereby activating or repressing the transcription of target genes (Wray et al., 2003). Gene specific transcription factors are DNA-binding regulatory proteins that activate or repre ...
... Transcription factors are proteins that modulate gene expression by binding to specific cis-acting promoter elements, thereby activating or repressing the transcription of target genes (Wray et al., 2003). Gene specific transcription factors are DNA-binding regulatory proteins that activate or repre ...
The Microbial Ecology of Dental Caries - Co
... Microbial Ecology in Health and Disease 2000; 12: 138–148 The microbial ecology of caries is complex and incorporates mechanisms common to the natural colonization of the host and to plaque accumulation at sites that do not develop caries lesions. It is not simply accumulation of plaque in the oral ...
... Microbial Ecology in Health and Disease 2000; 12: 138–148 The microbial ecology of caries is complex and incorporates mechanisms common to the natural colonization of the host and to plaque accumulation at sites that do not develop caries lesions. It is not simply accumulation of plaque in the oral ...
Genomic analysis of clinical samples with serologic ABO blood
... were used to amplify allele-specific fragments covering exon 6, intron 6, and exon 7 of the ABO gene under the following cycling conditions: Initial denaturation at 96°C for 10 minutes followed by 15 cycles at 94°C for 20 seconds, 65°C for 2.5 minutes, then 25 cycles at 94°C for 20 seconds, 61°C for ...
... were used to amplify allele-specific fragments covering exon 6, intron 6, and exon 7 of the ABO gene under the following cycling conditions: Initial denaturation at 96°C for 10 minutes followed by 15 cycles at 94°C for 20 seconds, 65°C for 2.5 minutes, then 25 cycles at 94°C for 20 seconds, 61°C for ...
RIVM rapport 601500005 The ecological risks of antibiotic
... and surface waters. The consequences for ecosystems are however difficult to assess, RIVM concluded in a literature review ordered by the Centre for Water Management. RIVM investigated the environmental risks of antibiotic resistance genes in aquatic environments. Resistance genes render bacteria in ...
... and surface waters. The consequences for ecosystems are however difficult to assess, RIVM concluded in a literature review ordered by the Centre for Water Management. RIVM investigated the environmental risks of antibiotic resistance genes in aquatic environments. Resistance genes render bacteria in ...
This article appeared in a journal published by Elsevier. The... copy is furnished to the author for internal non-commercial research
... for another 150 days to monitor their survival pattern. Change of bacterial community diversity by BS3 was detected by both cultivation based and cultivation independent (PCR-Denaturing Gradient Gel Electrophoresis) methods. In general, progressively increased BS3 concentration resulted in decreased ...
... for another 150 days to monitor their survival pattern. Change of bacterial community diversity by BS3 was detected by both cultivation based and cultivation independent (PCR-Denaturing Gradient Gel Electrophoresis) methods. In general, progressively increased BS3 concentration resulted in decreased ...
Article interaction G x tabac - Hal-CEA
... were detected for asthma by the MIT but not by the PST. These latter results might indicate presence of linkage without G x ETS interaction in these two regions. However, linkage analyses of asthma in the same EGEA data, ignoring G x E interaction, did not show evidence for linkage to these regions. ...
... were detected for asthma by the MIT but not by the PST. These latter results might indicate presence of linkage without G x ETS interaction in these two regions. However, linkage analyses of asthma in the same EGEA data, ignoring G x E interaction, did not show evidence for linkage to these regions. ...
Methods Five 1536-SNP GoldenGate assays (Fig. 1) Three pilot
... Residual SNPs from the three sources for POPA1 and POPA2 were used for POPA3, but additional SNPs were required. Additional SNPs came from three sources: 1) an extended list of 5732 SNPs from SCRI (NR) derived from reanalysis of the amplicon sequence alignments used for POPA1 and POPA2, 2) a new Har ...
... Residual SNPs from the three sources for POPA1 and POPA2 were used for POPA3, but additional SNPs were required. Additional SNPs came from three sources: 1) an extended list of 5732 SNPs from SCRI (NR) derived from reanalysis of the amplicon sequence alignments used for POPA1 and POPA2, 2) a new Har ...
Comparative analysis of two-component signal transduction systems
... The B. cereus group appeared to share as many as 20 orthologous HK-RR pairs and six ‘orphans’ with B. subtilis. Not all these HKs and RRs were found in every single B. cereus group genome. For example, the well-characterized B. subtilis HK CheA is absent from B. anthracis. In contrast, the well-char ...
... The B. cereus group appeared to share as many as 20 orthologous HK-RR pairs and six ‘orphans’ with B. subtilis. Not all these HKs and RRs were found in every single B. cereus group genome. For example, the well-characterized B. subtilis HK CheA is absent from B. anthracis. In contrast, the well-char ...
2004-009_-Draft-Anne..
... black in pears, but remain attached to the tree for some time. Upon infection young fruitlets turn brown but also remain attached to the tree. Immature fruit lesions appear oily or water-soaked, becoming brown to black and often exuding droplets of bacterial ooze. Characteristic reddish-brown streak ...
... black in pears, but remain attached to the tree for some time. Upon infection young fruitlets turn brown but also remain attached to the tree. Immature fruit lesions appear oily or water-soaked, becoming brown to black and often exuding droplets of bacterial ooze. Characteristic reddish-brown streak ...
The Importance of Biofilms in Chronic Rhinosinusitis Chapter 8
... Centers for Disease Control and Prevention estimates that ∼60–70% of all infections are biofilm related. As more focus is directed to microbes growing in the biofilm lifestyle, it is likely that other diseases will be indentified that clearly have biofilm links. ...
... Centers for Disease Control and Prevention estimates that ∼60–70% of all infections are biofilm related. As more focus is directed to microbes growing in the biofilm lifestyle, it is likely that other diseases will be indentified that clearly have biofilm links. ...
SVPaper050711
... Classification of SVs and their size characteristics are summarized in Figure 3. SVs are most often due to retrotransposons (LINEs (25%), LTRs (14%) and SINEs (15%)), followed by variable number tandem repeats (VNTRs) (15%) and pseudogenes (2%). Other mechanisms, not involving retrotransposons, acco ...
... Classification of SVs and their size characteristics are summarized in Figure 3. SVs are most often due to retrotransposons (LINEs (25%), LTRs (14%) and SINEs (15%)), followed by variable number tandem repeats (VNTRs) (15%) and pseudogenes (2%). Other mechanisms, not involving retrotransposons, acco ...
Identification of New Genes Involved in Meiosis by a Genetic Screen
... The yeast spore wall is a thick four-layered structure. Dityrosine is a major component of the spore wall that provides resistance against digestive enzymes such as glusalase, as well as UV and are present at the outermost and last synthesized layer of the spore wall (Briza et al., 1986; Pammer et a ...
... The yeast spore wall is a thick four-layered structure. Dityrosine is a major component of the spore wall that provides resistance against digestive enzymes such as glusalase, as well as UV and are present at the outermost and last synthesized layer of the spore wall (Briza et al., 1986; Pammer et a ...
Slide 1
... Total Balls: total number of genes considered (but a gene must be analyzed in both experiments: exclude those studied in only one) Black Balls: all genes found in experiment X White Balls: all genes not found in experiment X Sample: all genes found in experiment Y Lecture 3: Univariate Analyses II: ...
... Total Balls: total number of genes considered (but a gene must be analyzed in both experiments: exclude those studied in only one) Black Balls: all genes found in experiment X White Balls: all genes not found in experiment X Sample: all genes found in experiment Y Lecture 3: Univariate Analyses II: ...
Conservation of Brachyury (T) genes in amphioxus and vertebrates
... at the single cell level, revealed a significant difference to the expression of mouse, Xenopus, chick and zebrafish Brachyury. As-T RNA is restricted to presumptive notochord cells, commencing after their fate is restricted to notochord only; As-T transcripts are not detectable in cells fated to gi ...
... at the single cell level, revealed a significant difference to the expression of mouse, Xenopus, chick and zebrafish Brachyury. As-T RNA is restricted to presumptive notochord cells, commencing after their fate is restricted to notochord only; As-T transcripts are not detectable in cells fated to gi ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.