Wolbachia–host interactions: connecting phenotype to genotype
... parasites of insects is becoming complicated as an increasing number of papers describe a richer picture of Wolbachia-mediated phenotypes in insects. The search for the molecular basis for this phenotypic variability has been greatly aided by the recent sequencing of several Wolbachia genomes. These ...
... parasites of insects is becoming complicated as an increasing number of papers describe a richer picture of Wolbachia-mediated phenotypes in insects. The search for the molecular basis for this phenotypic variability has been greatly aided by the recent sequencing of several Wolbachia genomes. These ...
Periodic Selection and Ecological Diversity in Bacteria
... In the absence of recombination, the adaptive mutation is unable to enter into any other genetic background, and so selection favoring the adaptive mutation drags the entire genome associated with it to fixation. A recent reenactment of this experiment, supported by data from modern molecular biolog ...
... In the absence of recombination, the adaptive mutation is unable to enter into any other genetic background, and so selection favoring the adaptive mutation drags the entire genome associated with it to fixation. A recent reenactment of this experiment, supported by data from modern molecular biolog ...
Supplementary Materials and methods (doc 154K)
... reactions was calculated from the standard curve generated by performing qPCR with 4 5-fold dilutions of template DNAs in triplicate (~2 ng/μl to 16 pg/μl working range of DNA concentration). qPCRs were performed using a SensiMix SYBR Hi-ROX Kit ...
... reactions was calculated from the standard curve generated by performing qPCR with 4 5-fold dilutions of template DNAs in triplicate (~2 ng/μl to 16 pg/μl working range of DNA concentration). qPCRs were performed using a SensiMix SYBR Hi-ROX Kit ...
Complementary hierarchical clustering
... and Gordon (1999). Cluster analysis is an unsupervised learning procedure with the goal of grouping data into clusters, with members within a cluster being closer to each other than to members outside that cluster. In order to quantify how close one data point is to another, a distance measure is re ...
... and Gordon (1999). Cluster analysis is an unsupervised learning procedure with the goal of grouping data into clusters, with members within a cluster being closer to each other than to members outside that cluster. In order to quantify how close one data point is to another, a distance measure is re ...
IMPROVE SMALL RNA-MEDIATED GENE SILENCING
... RNA silencing is one of the gene regulatory mechanisms conserved among almost all eukaryotic organisms, which refers to a collection of RNA-mediated sequence-specific inhibition of gene expression, either at the post-transcriptional or transcriptional level (Frizzi and Huang 2010). The phenomenon of ...
... RNA silencing is one of the gene regulatory mechanisms conserved among almost all eukaryotic organisms, which refers to a collection of RNA-mediated sequence-specific inhibition of gene expression, either at the post-transcriptional or transcriptional level (Frizzi and Huang 2010). The phenomenon of ...
Chapter 4
... a DNA sequence that is consequently translated into a protein by encoding each triplet of nucleotides into an amino acid. The key to introducing frameshift lies in this triplet-based structure also known as the reading frame. Any insertion of deletion with a length not divisible by three introduces ...
... a DNA sequence that is consequently translated into a protein by encoding each triplet of nucleotides into an amino acid. The key to introducing frameshift lies in this triplet-based structure also known as the reading frame. Any insertion of deletion with a length not divisible by three introduces ...
document
... design 2 oligos which amplified the partial COMT gene from the bm3 and non bm3 lines, and then these fragments were then cloned and sent to Cogenics to obtain the full length sequence of ~1800 bases. The bm3 lines tested all contain a large deletion mutation at the 3’ exon of the COMT gene and this ...
... design 2 oligos which amplified the partial COMT gene from the bm3 and non bm3 lines, and then these fragments were then cloned and sent to Cogenics to obtain the full length sequence of ~1800 bases. The bm3 lines tested all contain a large deletion mutation at the 3’ exon of the COMT gene and this ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
NIH Public Access
... Since biochemical analysis of such a large, tightly chromatin bound RNA has been difficult by standard extraction-based approaches, we pursued a different approach to manipulate the binding or release of XIST RNA directly within living cells. This strategy built upon a significant clue from our earl ...
... Since biochemical analysis of such a large, tightly chromatin bound RNA has been difficult by standard extraction-based approaches, we pursued a different approach to manipulate the binding or release of XIST RNA directly within living cells. This strategy built upon a significant clue from our earl ...
1 - IPPC
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
Thesis Sara Domingues - Estudo Geral
... pathways for horizontal dissemination in bacteria. Mob Genet Elements 2(5):113. Material from this publication appears in the Introduction and Results chapters. ...
... pathways for horizontal dissemination in bacteria. Mob Genet Elements 2(5):113. Material from this publication appears in the Introduction and Results chapters. ...
Symbiotic Conversations Are Revealed Under Genetic Interrogation
... interactions function in the context in which they evolved. In contrast to the genetically modified or inbred host lines that are developed for constructed systems, natural models purposely use a population of genetically heterogeneous hosts to provide insight into the natural range of responses tha ...
... interactions function in the context in which they evolved. In contrast to the genetically modified or inbred host lines that are developed for constructed systems, natural models purposely use a population of genetically heterogeneous hosts to provide insight into the natural range of responses tha ...
Rapid Microscope Based Identification Method for Tuberculosis and
... identification of different species of microorganisms without prior cultivation. This technique (oligonucleotide FISH) was then further developed and applied by Amann et al in the early 1990s on a variety of environmental samples [30]. Some of the first oligonucleotide FISH applications on medical s ...
... identification of different species of microorganisms without prior cultivation. This technique (oligonucleotide FISH) was then further developed and applied by Amann et al in the early 1990s on a variety of environmental samples [30]. Some of the first oligonucleotide FISH applications on medical s ...
arXiv:0708.2724v1 [cond-mat.other] 20 Aug 2007
... of biology by, e.g., comparing genomes between species to find common properties and functions. This information could be used both medically (e.g., in animal testing of potential cures for humans) and also to examine the evolutionary heritage of all species. In order to reach the goal of a rapid an ...
... of biology by, e.g., comparing genomes between species to find common properties and functions. This information could be used both medically (e.g., in animal testing of potential cures for humans) and also to examine the evolutionary heritage of all species. In order to reach the goal of a rapid an ...
RT2 Profiler™ PCR Arrays: Pathway
... of each primer set is measured against the entire human, mouse, or rat genome to prevent the amplification of sequence-related, non-specific secondary products. The primer specificity is also checked against the E. coli genome to assure that the primers do not amplify bacterial genomic DNA, a common bu ...
... of each primer set is measured against the entire human, mouse, or rat genome to prevent the amplification of sequence-related, non-specific secondary products. The primer specificity is also checked against the E. coli genome to assure that the primers do not amplify bacterial genomic DNA, a common bu ...
Anti-‐Microbial Resistance
... Findings: A majority of commercially available molecular tests focus on detecting C. difficile, MRSA and VRE (i.e. vancomycin resistance markers, not VRE per se). A few platforms also offer tests for ...
... Findings: A majority of commercially available molecular tests focus on detecting C. difficile, MRSA and VRE (i.e. vancomycin resistance markers, not VRE per se). A few platforms also offer tests for ...
Disintegrin, hemorrhagic, and proteolytic activities of Mohave
... mg/kg). Both subunits must be present to produce Mojave toxin, which can increase the venom toxicity significantly. Although specimens of C. s. scutulatus sampled throughout the majority of its range contain Mojave toxin, there are populations in central Arizona that do not express the neurotoxic co ...
... mg/kg). Both subunits must be present to produce Mojave toxin, which can increase the venom toxicity significantly. Although specimens of C. s. scutulatus sampled throughout the majority of its range contain Mojave toxin, there are populations in central Arizona that do not express the neurotoxic co ...
Characterisation of the physical and chemical properties influencing
... on egg masses exhibited antibacterial activity in mixed culture and was identified as Bacillus ...
... on egg masses exhibited antibacterial activity in mixed culture and was identified as Bacillus ...
The Integrated Phenotype
... functional units (or modules) in the organism (Schlichting 1989; Pigliucci and Preston 2004). Proper functioning of complex phenotypes requires that traits work together within a module. Modularity describes the relative independence of complex traits. The interactions among characters may change th ...
... functional units (or modules) in the organism (Schlichting 1989; Pigliucci and Preston 2004). Proper functioning of complex phenotypes requires that traits work together within a module. Modularity describes the relative independence of complex traits. The interactions among characters may change th ...
A homozygous double mutation in SMN1
... SMN1 genes carry both the exon 1 and exon 5 mutations. His healthy parents (IV-3; IV-4) were found to be heterozygous for one mutant SMN1 allele, carrying both the exon 1 and exon 5 mutations and one normal allele. Prenatal testing for mutation detection was requested several months later when this ...
... SMN1 genes carry both the exon 1 and exon 5 mutations. His healthy parents (IV-3; IV-4) were found to be heterozygous for one mutant SMN1 allele, carrying both the exon 1 and exon 5 mutations and one normal allele. Prenatal testing for mutation detection was requested several months later when this ...
ISOLATION AND IDENTIFICATION OF BURKHOLDERIA SPECEIS
... of Burkholderia species in Malaysia. Hence, this project was set out to detect and identify Burkholderia species in our environmental reservoirs, focusing on those around Kampar. Six soil samples and 12 water samples were collected from various locations, in which some involve direct human contact. ...
... of Burkholderia species in Malaysia. Hence, this project was set out to detect and identify Burkholderia species in our environmental reservoirs, focusing on those around Kampar. Six soil samples and 12 water samples were collected from various locations, in which some involve direct human contact. ...
Pristionchus pacificus
... with permission from Hong and Sommer (2006), Bioessays © copyright 2006 Wiley Periodicals, Inc. Number of Ppa SNP ...
... with permission from Hong and Sommer (2006), Bioessays © copyright 2006 Wiley Periodicals, Inc. Number of Ppa SNP ...
Characterization and resistance profiles of selected
... age; with isolates from older animals being less resistant. This resistance profile was similar in all 3 groups in both rehabilitation centers. Establishing the presence of microbial exchange between human populations and wildlife (especially primates), even in the absence of immediate disease conce ...
... age; with isolates from older animals being less resistant. This resistance profile was similar in all 3 groups in both rehabilitation centers. Establishing the presence of microbial exchange between human populations and wildlife (especially primates), even in the absence of immediate disease conce ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.