MATRILINEAL, a sperm-specific phospholipase, triggers maize
... process1. Costly, genotype-dependent tissue culture methods are used in many crops2, while seed-based in vivo doubled haploid systems are rare in nature3 and difficult to manage in breeding programmes4. The multi-billion-dollar maize hybrid seed business, however, is supported by industrial doubled ...
... process1. Costly, genotype-dependent tissue culture methods are used in many crops2, while seed-based in vivo doubled haploid systems are rare in nature3 and difficult to manage in breeding programmes4. The multi-billion-dollar maize hybrid seed business, however, is supported by industrial doubled ...
Phage Renaissance: New Hope against
... “Before this, no formal study was ever done in the United States to show the safety of phage cocktails,” says Sulakvelidze. Funding is needed to proceed to phase 2 trials to evaluate how well the treatment works. Nelson and colleagues at Rockefeller University in New York City have purified lysins t ...
... “Before this, no formal study was ever done in the United States to show the safety of phage cocktails,” says Sulakvelidze. Funding is needed to proceed to phase 2 trials to evaluate how well the treatment works. Nelson and colleagues at Rockefeller University in New York City have purified lysins t ...
DEPARTAMENT DE GENÈTICA GENOME EVOLUTION AND SYSTEMS BIOLOGY IN
... These advances have generated a change in the scope of prokaryotic genome sequencing projects, from the sequencing of representative isolates culturable bacteria, mainly important human pathogens, to metagenomic studies in which the objective is the sequencing of entire microbial communities of a gi ...
... These advances have generated a change in the scope of prokaryotic genome sequencing projects, from the sequencing of representative isolates culturable bacteria, mainly important human pathogens, to metagenomic studies in which the objective is the sequencing of entire microbial communities of a gi ...
Differential Enzyme Targeting As an Evolutionary Adaptation to
... ancestral translation start sites (1 and 2 in figure 3A) in all seven species, as predicted from the observation that at least some AGT is mitochondrial in all cases. An alignment (fig. 3B) of the 55–amino acid sequence encoded by exon 1 downstream of, and including, the second translation start sit ...
... ancestral translation start sites (1 and 2 in figure 3A) in all seven species, as predicted from the observation that at least some AGT is mitochondrial in all cases. An alignment (fig. 3B) of the 55–amino acid sequence encoded by exon 1 downstream of, and including, the second translation start sit ...
A Guide to Conducting Systematic Reviews in Agri-Food
... organizations and agencies have addressed these concerns through an increased scope and intensity of targeted surveillance, the development and implementation of intervention strategies throughout the food chain, the integration of risk assessment into policy development and through increased fundin ...
... organizations and agencies have addressed these concerns through an increased scope and intensity of targeted surveillance, the development and implementation of intervention strategies throughout the food chain, the integration of risk assessment into policy development and through increased fundin ...
this PDF file - Microbial Ecology in Health and Disease
... (9, 28, 29). Harmsen et al. compared newer techniques (FISH, 16S rRNA probes) with culturing techniques and found high counts were found by culturing only after 8 –9 days, but FISH detected anaerobes by the second day (30). In addition, another study indicated that neonates can acquire strict anaero ...
... (9, 28, 29). Harmsen et al. compared newer techniques (FISH, 16S rRNA probes) with culturing techniques and found high counts were found by culturing only after 8 –9 days, but FISH detected anaerobes by the second day (30). In addition, another study indicated that neonates can acquire strict anaero ...
Effect of the Polymorphisms of Keratin Associated Protein 8.2 Gene
... cashmere fibre diameter; in long-term artificial fertilization and selection the excellent trait is always primarily preserved. The sequencing results showed that two unreported mutations, A214G and T218C were identified in the KAP8.2 gene. A214G did not change the amino acid, while T218C led to an ...
... cashmere fibre diameter; in long-term artificial fertilization and selection the excellent trait is always primarily preserved. The sequencing results showed that two unreported mutations, A214G and T218C were identified in the KAP8.2 gene. A214G did not change the amino acid, while T218C led to an ...
- Academy Test Bank
... Learning Outcome: 03.03.01 Describe the fluid mosaic model of membrane structure and identify the types of lipids typically found in bacterial membranes Learning Outcome: 03.05.01 Compile a list of the structures found in all the layers of bacterial cell envelopes, noting the functions and the major ...
... Learning Outcome: 03.03.01 Describe the fluid mosaic model of membrane structure and identify the types of lipids typically found in bacterial membranes Learning Outcome: 03.05.01 Compile a list of the structures found in all the layers of bacterial cell envelopes, noting the functions and the major ...
Gene expression analysis
... Why find genes that behave differently in two classes (e.g. normal and tumor)? Better understanding of the genetic circumstances that cause the difference (disease) hopefully leads to better therapy. Detection of marker-genes enables the early recognition of diseases as well as the recognition of su ...
... Why find genes that behave differently in two classes (e.g. normal and tumor)? Better understanding of the genetic circumstances that cause the difference (disease) hopefully leads to better therapy. Detection of marker-genes enables the early recognition of diseases as well as the recognition of su ...
Identity elements in tRNA-mediated transcription
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
... system. An uncharged tRNA can bind to the leader transcript, maintain the antiterminator conformation and thus promote the expression of the operon or gene. A tRNA that is charged with an amino acid on the acceptor arm, however, would not be able to bind and stabilize the antiterminator in the leade ...
PHI-Canto video tutorial slides - PHI-base
... Our vision Typical Paper: Curation of a single gene deletion in a pathogen tested on two hosts and some in vitro characterisation - Take 15 mins Start with a Pubmed ID use the duplication function manual checking as the annotation extension is built More complex paper: Curation of a single and doub ...
... Our vision Typical Paper: Curation of a single gene deletion in a pathogen tested on two hosts and some in vitro characterisation - Take 15 mins Start with a Pubmed ID use the duplication function manual checking as the annotation extension is built More complex paper: Curation of a single and doub ...
Slide 1
... Tetracycline Resistance Genes in Oral Bacteria. Antimicrobial Agents and Chemotherapy, 47(3), 878-882. White, D.G.; Zhao, S.; Simjee, S.; Wagner, D. D.; McDermott, P. F. (2002) Antimicrobial resistance of foodborne ...
... Tetracycline Resistance Genes in Oral Bacteria. Antimicrobial Agents and Chemotherapy, 47(3), 878-882. White, D.G.; Zhao, S.; Simjee, S.; Wagner, D. D.; McDermott, P. F. (2002) Antimicrobial resistance of foodborne ...
New variants of the tet(M) gene in Clostridium
... tet(M)1, 2, 3 and 4 variants were found in C. difficile isolates resistant or inducibly resistant to tetracycline, and tet(M)5 and 6 were found in susceptible isolates. The organization of the element carrying tet(M)3, tet(M)6 and tet(M)1 of cd12 was similar to that of E. faecalis DS16. This analysi ...
... tet(M)1, 2, 3 and 4 variants were found in C. difficile isolates resistant or inducibly resistant to tetracycline, and tet(M)5 and 6 were found in susceptible isolates. The organization of the element carrying tet(M)3, tet(M)6 and tet(M)1 of cd12 was similar to that of E. faecalis DS16. This analysi ...
Bacterial diversity and viral discovery in the invasive Argentine ant
... core of nine bacteria genera, including Lactobacillus and Gluconobacter, two bacterial genera with members known for their beneficial associations with honey bees. The presence of these core bacteria may have participated in the success of Argentine ants in their new environments. In the laboratory ...
... core of nine bacteria genera, including Lactobacillus and Gluconobacter, two bacterial genera with members known for their beneficial associations with honey bees. The presence of these core bacteria may have participated in the success of Argentine ants in their new environments. In the laboratory ...
Real-time PCR-based detection of Bordetella pertussis and
... Novel real-time PCR assays targeting the Bordetella pertussis insertion sequence IS481, the toxin promoter region and Bordetella parapertussis insertion sequence IS1001 were designed. PCR assays were capable of detecting ¡10 copies of target DNA per reaction, with an amplification efficiency of ¢90 ...
... Novel real-time PCR assays targeting the Bordetella pertussis insertion sequence IS481, the toxin promoter region and Bordetella parapertussis insertion sequence IS1001 were designed. PCR assays were capable of detecting ¡10 copies of target DNA per reaction, with an amplification efficiency of ¢90 ...
Press release
... Many breeding programs involve a significant increase in the number of samples, which can be technically challenging for breeders if they are to analyse plants rapidly and precisely. The first step in the selection process consists of purifying the DNA, which needs to be purified for analysis or, in ...
... Many breeding programs involve a significant increase in the number of samples, which can be technically challenging for breeders if they are to analyse plants rapidly and precisely. The first step in the selection process consists of purifying the DNA, which needs to be purified for analysis or, in ...
Epiphytic Planctomycetes from macroalgae
... to the novel taxa under study, the application of a different molecular marker was applied to the Planctomycetes group as a complement of the 16S rRNA gene and as a possible substitute of DNA-DNA hybridization (DDH). DDH is required for species description when the 16S rRNA gene similarity between t ...
... to the novel taxa under study, the application of a different molecular marker was applied to the Planctomycetes group as a complement of the 16S rRNA gene and as a possible substitute of DNA-DNA hybridization (DDH). DDH is required for species description when the 16S rRNA gene similarity between t ...
Chromosomal translocations deregulated BCL6
... including the Drosophila developmental regulators Tramtrack and Broad-complex (Harrison and Travers, 1990; DiBello et al., 1991), as well as the human KUP (Chardin et al., 1991), ZID (Bardwell and Treisman, 1994) and PLZF (Chen et al., 1993) proteins. The BCL6 gene is tightly regulated during B cell ...
... including the Drosophila developmental regulators Tramtrack and Broad-complex (Harrison and Travers, 1990; DiBello et al., 1991), as well as the human KUP (Chardin et al., 1991), ZID (Bardwell and Treisman, 1994) and PLZF (Chen et al., 1993) proteins. The BCL6 gene is tightly regulated during B cell ...
Attachment 2.2 Sequencing results
... On the other hand, AHR deficient mice have a reduced body weight, display reduced fecundity and are defective in development of both liver and immune system. In these mice fetal vascular structures are found in the eyes, kidneys and liver. [1] Objective: to study the conservation of the AHR gene and ...
... On the other hand, AHR deficient mice have a reduced body weight, display reduced fecundity and are defective in development of both liver and immune system. In these mice fetal vascular structures are found in the eyes, kidneys and liver. [1] Objective: to study the conservation of the AHR gene and ...
Introduction to GO Annotation
... protein to the plasma membrane supports the prediction that PERK1 is an integral membrane protein…these kinases have been implicated in early stages of wound response… ...
... protein to the plasma membrane supports the prediction that PERK1 is an integral membrane protein…these kinases have been implicated in early stages of wound response… ...
designing a biosensor that will detect gram negative and gram
... The tremendous diversity of prokaryotes has made bacterial speciation a very difficult task. From an evolution point of view, the high rate of recombination that resulted from genes acquisition, loss or transfer between bacteria has created various phenotypes and DNA sequences9. This genetic exchang ...
... The tremendous diversity of prokaryotes has made bacterial speciation a very difficult task. From an evolution point of view, the high rate of recombination that resulted from genes acquisition, loss or transfer between bacteria has created various phenotypes and DNA sequences9. This genetic exchang ...
Product description P003-D1 MLH1-MSH2-v01 - MRC
... Reference samples: Reference DNA samples should be derived from the same tissue type, handled using the same procedure, and prepared using the same DNA extraction method as the patient samples. Reference samples should be derived from unrelated individuals who are from families without a history of ...
... Reference samples: Reference DNA samples should be derived from the same tissue type, handled using the same procedure, and prepared using the same DNA extraction method as the patient samples. Reference samples should be derived from unrelated individuals who are from families without a history of ...
Recombination in large RNA viruses: Coronaviruses
... Experimental evidence in tissue culture indicated that recombination can generate new viruses, even when no specific selection pressures were applied, as long as these recombinants have evolutionary advantages.8 The effects of recombination on the evolution of coronavirus DI RNAs have also been demo ...
... Experimental evidence in tissue culture indicated that recombination can generate new viruses, even when no specific selection pressures were applied, as long as these recombinants have evolutionary advantages.8 The effects of recombination on the evolution of coronavirus DI RNAs have also been demo ...
Assessing the role of anammox in a nitrogen contaminated aquifer
... Since their discovery anammox bacteria have been known as slow-growers, with a division time of once every 11-20 days (Strous et al., 1999). Escherichia coli, for example, divide once every 20 or so minutes. The anammox bacteria are obligate anaerobes and cannot tolerate oxygen conditions above 2 μM ...
... Since their discovery anammox bacteria have been known as slow-growers, with a division time of once every 11-20 days (Strous et al., 1999). Escherichia coli, for example, divide once every 20 or so minutes. The anammox bacteria are obligate anaerobes and cannot tolerate oxygen conditions above 2 μM ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.