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::: Gene Set Enrichment Analysis - GSEA
::: Gene Set Enrichment Analysis - GSEA

... Detects significant functions with Gene Ontology InterPro motifs, Swissprot KW and KEGG pathways in lists of genes ordered according to differents characteristics. ...
SALSA MLPA probemix P222-A2 LCA mix-2 - MRC
SALSA MLPA probemix P222-A2 LCA mix-2 - MRC

... polymorphism in the sequence detected by a probe can also cause a reduction in relative peak area, even when not located exactly on the ligation site! In addition, some probe signals are more sensitive to sample purity and small changes in experimental conditions. Therefore, deletions and duplicatio ...
Analysis of the Molecular Basis of Flowering Time Variation in
Analysis of the Molecular Basis of Flowering Time Variation in

... alleles are fully functional and confer very late flowering in the presence of the strong Col FLC allele. Given the identical amino acid sequence, it is likely that the three accessions Shakhdara, Kondara, and Kz-9 carry the same functional FRI allele. The F1 plants from the cross of Kz-9 to FRI(Sf2 ...
The Normal Uniform Differential Gene Expression
The Normal Uniform Differential Gene Expression

... method versus the log intensities, lI, (along with the loess fitted mean lines of both). > #lRnorm is the mean and variance normalized log ratios for the genes ...
Phenotype Curation Tool and Ontologies at dictyBase
Phenotype Curation Tool and Ontologies at dictyBase

... • Developed in collaboration with NCBO • Similar in functionality to Phenote • Web-based tool that can be accessed from any computer ...
Additional file 1
Additional file 1

... is “cacgtg”. Therefore, we initially assign “cacgtg” as the DNA binding sites of bh2b. The same search is performed on Ipmyb1, but no clear consensus can be draw from Jaspar database. The following search is based on the assumption that the binding sites of two collaborative TFs are not far apart (e ...
A survey on computational methods for enhancer and
A survey on computational methods for enhancer and

... Regions with a good match to a sequence motif could be binding sites of the TF. Excluding binding sites at annotated regions such as promoters, the remaining could be CRMs, especially for regions with a high density of motif matches (Su et al. 2010). Since TF binding also depends on factors other th ...
ABSTRACT Title of Document:
ABSTRACT Title of Document:

... Phyllosphere Results Eight bacterial phyla and 14 classes were found in this environment. A statistically significant difference between organically and conventionally managed phyllosphere bacterial microbial communities was observed at four of six sampling time points. Unique phylotypes were found ...
Lecture 3 & 4
Lecture 3 & 4

... The common ancestor of all three domains had DNA; and its machinery for transcription and translation produced RNA and proteins; the chromosome was probably circular. Archaea and Eukarya share a more recent common ancestor with each other than with Bacteria. ...
Monsieur Matthieu MILLION Caractérisation des altérations du
Monsieur Matthieu MILLION Caractérisation des altérations du

... The revolution of large scale molecular sequencing methods allowed the identification of specific alterations in the gut microbiota associated with obesity such as a decreased Bacteroidetes / Firmicutes ratio in obese individuals. Since then, many studies have described different alterations associa ...
Genomics of the Bacillus cereus group of organisms
Genomics of the Bacillus cereus group of organisms

... whether B. cereus, B. thuringiensis and B. anthracis belong to one unique species and are variants of that species. The simplest markers used for phenotypic differentiation of these species are all encoded on plasmids (i.e., the pXO2 encoded capsule gene cluster) [19]. The fact that plasmids can eas ...
Bioinformatics - cs@union
Bioinformatics - cs@union

... …conceptualizing biology in terms of molecules (in the sense of physical chemistry) and applying “informatics techniques” (derived from disciplines such as applied maths, computer science and statistics) to understand and organize the information associated with these molecules, on a large scale. In ...
Identification, evolution, and spread of bacterial virulence
Identification, evolution, and spread of bacterial virulence

... The quest for virulence genes evolved together with the technical development of molecular biology and genetic modification of microorganisms. In the beginning of molecular microbiology, genes were identified that coded for virulence factors of known reputation. These virulence genes were then used ...
TaqMan-Based Real-Time PCR for Genotyping Common
TaqMan-Based Real-Time PCR for Genotyping Common

... and HP2 for typing (14, 15 ). More recently, a singlechain antibody-based ELISA test was also established (16 ). In addition, several HP-genotyping methods based on conventional PCR have been developed; however, these PCR-based methods need to amplify a relatively large DNA fragment to detect the 1. ...
documentation in PDF format
documentation in PDF format

... GENCODE transcript annotations that will be useful to the majority of users. GENCODE Basic includes all genes in the GENCODE gene set, with a representative subset of the transcripts (splice variants). You can also select to use RefSeq transcripts from the otherfeatures database; note though that th ...
SNP Discovery and Genotyping Workshop (PowerPoint)
SNP Discovery and Genotyping Workshop (PowerPoint)

... Where do I find SNPs to use in my association studies? (e.g. databases, direct resequencing) 2. SNP Selection: How do I choose SNPs that are informative? (i.e. assessing SNP correlation - linkage disequilibrium) 3. SNP Associations: What analyses can I perform after genotyping these SNPs? (e.g. sing ...
Chapter 26: Bacteria and Archaea: the Prokaryotic Domains
Chapter 26: Bacteria and Archaea: the Prokaryotic Domains

... which many species obtain energy for generating ATP by oxidizing reduced organic compounds Highly reduced molecule, glucose, serves as original electron donor (ie, molecule is oxidized) and highly oxidized molecule, oxygen, serves as ...
i Antibiotic Susceptibility Patterns and Detection of Genes
i Antibiotic Susceptibility Patterns and Detection of Genes

... diffusion method. Polymerase Chain Reaction (PCR) was used to screen for the presence of aminoglycoside modifying enzymes (ame) and integron 1 and 2 genes (int1 and int2). Presence of plasmid mediated resistance was also screened by isolation of plasmids from multi resistant isolates and the plasmid ...
Abstracts - Parthenon Management Group
Abstracts - Parthenon Management Group

... of schizophrenia to carriers as well as to other neurodevelopmental phenotypes including ADHD, Autism and Intellectual Disability. Early whole-exome sequencing studies have not yet identified additional variation, but show some promise to do so. Although only a small amount of the total genetic vari ...
Differentiation of Heliobacter pylori using PCR-RFLP
Differentiation of Heliobacter pylori using PCR-RFLP

... Helicobacter pylori is one of the most common human pathogens and since it was discovered in 1982 by Marshall and Warren has been established as one of the most variable and diverse bacteria known to man. Resident to the lining of the human stomach it is seen as the cause of a multitude of illnesses ...
SeqScape® Software 3 User Guide
SeqScape® Software 3 User Guide

... only to perform internal research for the sole benefit of the purchaser. No right to resell this product or any of its components is conveyed expressly, by implication, or by estoppel. This product is for internal research purposes only and is not for use in commercial applications of any kind, incl ...
The prokaryotes
The prokaryotes

... N-free media. However, traces of fixed nitrogen in the media sometimes accounted for the bacterial growth. At other times, oligotrophic bacteria and fungi, which can grow on N-free media, have been incorrectly reported to be N2-fixing organisms. These microorganisms appear to meet their nitrogen req ...
A MIAME-compliant Microarray Database
A MIAME-compliant Microarray Database

... • Let’s cluster them over our time course in the second experiment. ...
Virp1 Is a Host Protein with a Major Role in Potato - IMBB
Virp1 Is a Host Protein with a Major Role in Potato - IMBB

... Received 6 May 2007/Accepted 16 September 2007 ...
Physiology and Molecular Characterization of Microbial
Physiology and Molecular Characterization of Microbial

... In northern Alberta, mining operations to obtain bitumen from the oil sands generates large volumes of tailings. These are a mixture of sand, clay, water, organic solvents and residual bitumen that are deposited into old open pits, creating tailings ponds, where they are allowed to settle with the f ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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