Standards and guidelines for the interpretation of sequence
... that have developed variant classification guidelines for wellstudied genes in breast cancer, colon cancer, and cystic fibrosis and statistical analysis programs for quantitative evaluation of variants in select diseases.2–5 While those variant analysis guidelines are useful in a specific setting, i ...
... that have developed variant classification guidelines for wellstudied genes in breast cancer, colon cancer, and cystic fibrosis and statistical analysis programs for quantitative evaluation of variants in select diseases.2–5 While those variant analysis guidelines are useful in a specific setting, i ...
Proteus mirabilis Tigecycline (GAR-936) in with Reduced Levels of
... Genomic DNA was prepared from LB broth cultures using the Puregene DNA isolation kit (Gentra Systems, Minneapolis, Minn.) according to the manufacturer’s instructions. DNA isolation and transposon mapping. Transposon mapping was performed by rescue cloning the transposon and flanking chromosomal DNA ...
... Genomic DNA was prepared from LB broth cultures using the Puregene DNA isolation kit (Gentra Systems, Minneapolis, Minn.) according to the manufacturer’s instructions. DNA isolation and transposon mapping. Transposon mapping was performed by rescue cloning the transposon and flanking chromosomal DNA ...
Visualizza/apri
... The research of pathogenic microorganisms in foods has seen an intensification of the efforts to develop more specific and sensitive methods able to ascertain the presence of any virus or bacteria potentially harmful for health before marketing the products. This thesis was developed on the basis of ...
... The research of pathogenic microorganisms in foods has seen an intensification of the efforts to develop more specific and sensitive methods able to ascertain the presence of any virus or bacteria potentially harmful for health before marketing the products. This thesis was developed on the basis of ...
Exceptionally high levels of recombination
... Institute of Genetics, Heinrich Heine Universität Duesseldorf, Duesseldorf 40225, Germany; 2Section of Evolutionary Biology, Department of Biology II, University of Munich, Planegg-Martinsried 82152, Germany; 3Department of Entomology, Purdue University, West Lafayette, Indiana 47907, USA; 4Populati ...
... Institute of Genetics, Heinrich Heine Universität Duesseldorf, Duesseldorf 40225, Germany; 2Section of Evolutionary Biology, Department of Biology II, University of Munich, Planegg-Martinsried 82152, Germany; 3Department of Entomology, Purdue University, West Lafayette, Indiana 47907, USA; 4Populati ...
A High Density Integrated Genetic Linkage Map of Soybean and the
... be the most abundant source of DNA polymorphisms in soybean (Hyten et al., 2006; Zhu et al., 2003). Despite being the most common molecular marker in soybean, the SNP frequency is relatively low compared to other cultivated crop species (Hyten et al., 2006; Zhu et al., 2003). The relatively low sequ ...
... be the most abundant source of DNA polymorphisms in soybean (Hyten et al., 2006; Zhu et al., 2003). Despite being the most common molecular marker in soybean, the SNP frequency is relatively low compared to other cultivated crop species (Hyten et al., 2006; Zhu et al., 2003). The relatively low sequ ...
FLUORESCENT PROTEIN IN Escherichia coli
... The discovery of penicillin in 1928 by Sir Alexander Fleming was a huge breakthrough and became a shift in paradigm to how infections were treated. Since then many other antiobiotics were developed, e.g. streptomycin against tuberculosis. This meant that the leading cause of death changed from being ...
... The discovery of penicillin in 1928 by Sir Alexander Fleming was a huge breakthrough and became a shift in paradigm to how infections were treated. Since then many other antiobiotics were developed, e.g. streptomycin against tuberculosis. This meant that the leading cause of death changed from being ...
Computational Definition of
... sequence families comprising 2069 putative exonic enhancers and 974 putative exonic silencers. Representatives of each class indeed functioned as enhancers or silencers when inserted into a test exon and assayed in transfected mammalian cells. As a class, the enhancer sequencers were more prevalent ...
... sequence families comprising 2069 putative exonic enhancers and 974 putative exonic silencers. Representatives of each class indeed functioned as enhancers or silencers when inserted into a test exon and assayed in transfected mammalian cells. As a class, the enhancer sequencers were more prevalent ...
Molecular Evolution of Overlapping Genes
... The method presented in Chapters Two, Three, and Four is limited to the analysis of sequences from divergent species. In some cases, the question of functionality is asked for an overlapping gene, which is unique to a population of clinically important viruses and bacteria. One such interesting case ...
... The method presented in Chapters Two, Three, and Four is limited to the analysis of sequences from divergent species. In some cases, the question of functionality is asked for an overlapping gene, which is unique to a population of clinically important viruses and bacteria. One such interesting case ...
Phylogenetic analysis of three complete gap
... S.D. Eastman et al. / Genomics 87 (2006) 265 – 274 Connexin genes are arranged in clades identifiable by similar colors used on phylogram tree (Fig. 1). Zebrafish connexin genes are subsequently organized by ascending molecular weight within a clade. ‘‘Previous name’’ indicates previous publication ...
... S.D. Eastman et al. / Genomics 87 (2006) 265 – 274 Connexin genes are arranged in clades identifiable by similar colors used on phylogram tree (Fig. 1). Zebrafish connexin genes are subsequently organized by ascending molecular weight within a clade. ‘‘Previous name’’ indicates previous publication ...
Bacteriophages of Soft Rot Enterobacteriaceae
... more related to each other than the strains that were resistant or more prone to be infected by different phages, hence could be identified (Anderson and Williams 1956). However, phage typing is seldom used nowadays due to its low resolution, tendency for false positive and false negative results, a ...
... more related to each other than the strains that were resistant or more prone to be infected by different phages, hence could be identified (Anderson and Williams 1956). However, phage typing is seldom used nowadays due to its low resolution, tendency for false positive and false negative results, a ...
Package ‘GenomicFeatures’ October 14, 2014
... Author M. Carlson, H. Pages, P. Aboyoun, S. Falcon, M. Morgan,D. Sarkar, M. Lawrence License Artistic-2.0 Description A set of tools and methods for making and manipulating transcript centric annotations. With these tools the user can easily download the genomic locations of the transcripts, exons a ...
... Author M. Carlson, H. Pages, P. Aboyoun, S. Falcon, M. Morgan,D. Sarkar, M. Lawrence License Artistic-2.0 Description A set of tools and methods for making and manipulating transcript centric annotations. With these tools the user can easily download the genomic locations of the transcripts, exons a ...
Alu repeat analysis in the complete human genome: trends and
... units separated by a poly ‘A’ stretch. The monomers, homologous to 7SL RNA, are absolutely identical except for a 30 bp insertion in the right monomer (Jelinek et al., 1980; Ullu and Tschudi, 1984). The 3 end of the Alu element has a long stretch of adenine residues, and is flanked by 4–10 bp of di ...
... units separated by a poly ‘A’ stretch. The monomers, homologous to 7SL RNA, are absolutely identical except for a 30 bp insertion in the right monomer (Jelinek et al., 1980; Ullu and Tschudi, 1984). The 3 end of the Alu element has a long stretch of adenine residues, and is flanked by 4–10 bp of di ...
Molecular cloning and biochemical characterization of a
... digested with EcoRI. After EcoRI digestion, high molecular weight DNA was fractionated on a sucrose gradient and used to prepare a genomic library in lambda EMBL 4 (Stratagene) according to the manufacturer's instructions. More than 2 105 phages were transferred to Hybond N nylon membrane (Amersha ...
... digested with EcoRI. After EcoRI digestion, high molecular weight DNA was fractionated on a sucrose gradient and used to prepare a genomic library in lambda EMBL 4 (Stratagene) according to the manufacturer's instructions. More than 2 105 phages were transferred to Hybond N nylon membrane (Amersha ...
Analysis of acid-induced asr gene promoter of Enterobacteriaceae
... asr mRNA is a long-lived and one of the most abundant RNA messages in E. coli cells at maximal levels of induction [5]. This was confirmed by mRNA expression profiling of E. coli cells grown in a supplemented minimal medium and subjected to acid shock [7]. Search for the trans-acting regulatory comp ...
... asr mRNA is a long-lived and one of the most abundant RNA messages in E. coli cells at maximal levels of induction [5]. This was confirmed by mRNA expression profiling of E. coli cells grown in a supplemented minimal medium and subjected to acid shock [7]. Search for the trans-acting regulatory comp ...
Multiplex in-vitro Detection using SERS
... Raman scattering (SERRS). This occurs when the analyte contains a chromophore close in energy to the exciting radiation. However, a dye label can also be used to achieve this enhancement if the chromophore is not present in the analyte itself. This combination of resonance with surface enhancement h ...
... Raman scattering (SERRS). This occurs when the analyte contains a chromophore close in energy to the exciting radiation. However, a dye label can also be used to achieve this enhancement if the chromophore is not present in the analyte itself. This combination of resonance with surface enhancement h ...
18.4 Bacteria and Archaea Kingdom Eubacteria Domain Bacteria
... • by binary fission. • exchange genes during conjugation= increases diversity. ...
... • by binary fission. • exchange genes during conjugation= increases diversity. ...
Visualizations of Microarray Data in Partek Genomics Suite 6.6
... PGS’s function Sort rows by prototype enables you to identify genes with similar expression profiles. For instance, if a gene with an interesting expression pattern has been detected, using Sort by prototype makes it possible to find other genes that have a similar pattern of intensity values. Altho ...
... PGS’s function Sort rows by prototype enables you to identify genes with similar expression profiles. For instance, if a gene with an interesting expression pattern has been detected, using Sort by prototype makes it possible to find other genes that have a similar pattern of intensity values. Altho ...
- Premier University of Technology
... would comprise of four theory papers including practicals. There would be 13 cores, 6 common and 5 interdisciplinary papers, making a total of 24 papers. The new course will commence from the academic session 2011-12. The syllabus has been prepared keeping in view the unique requirements of Diploma ...
... would comprise of four theory papers including practicals. There would be 13 cores, 6 common and 5 interdisciplinary papers, making a total of 24 papers. The new course will commence from the academic session 2011-12. The syllabus has been prepared keeping in view the unique requirements of Diploma ...
Rapid Chromatic Detection of Bacteria by Use of a New Biomimetic
... scientific research. Numerous technologies for reporting on bacterial presence have been developed (3, 5, 9, 25, 26). There are, however, limitations to existing bacterial detection techniques as rapid and generic approaches. Specifically, many bioanalytical techniques employed for pathogen detectio ...
... scientific research. Numerous technologies for reporting on bacterial presence have been developed (3, 5, 9, 25, 26). There are, however, limitations to existing bacterial detection techniques as rapid and generic approaches. Specifically, many bioanalytical techniques employed for pathogen detectio ...
RNA Splicing
... • Introns in protein coding genes are generally of 3 classes * nuclear pre-mRNA introns * Group I introns * Group II introns • Nuclear pre-mRNA introns are identified by the presence of GU-AG base sequence ...
... • Introns in protein coding genes are generally of 3 classes * nuclear pre-mRNA introns * Group I introns * Group II introns • Nuclear pre-mRNA introns are identified by the presence of GU-AG base sequence ...
Genome-wide analysis of the distribution of AP2/ERF transcription
... Background: Cabbage (Brassica oleracea) is one of the most important leaf vegetables grown worldwide. The entire cabbage genome sequence and more than fifty thousand proteins have been obtained to date. However a high degree of sequence similarity and conserved genome structure remain between cabbag ...
... Background: Cabbage (Brassica oleracea) is one of the most important leaf vegetables grown worldwide. The entire cabbage genome sequence and more than fifty thousand proteins have been obtained to date. However a high degree of sequence similarity and conserved genome structure remain between cabbag ...
Horizontal gene transfer from flowering plants to Gnetum
... b and c sequences are available from a range of seed plants, and full intron sequences for 18 additional angiosperm taxa were generated in our lab (Table 2). Gene Sequencing. We extracted DNA from silica gel-dried leaves ...
... b and c sequences are available from a range of seed plants, and full intron sequences for 18 additional angiosperm taxa were generated in our lab (Table 2). Gene Sequencing. We extracted DNA from silica gel-dried leaves ...
Male-killing i n two species of insect Wolbachia 1~ THE ROYAL
... bipunctata, and the butterfly Acraea encedon. Both male-killing bacteria belong to the B group of Wolbachia. However, phylogenetic analyses were unable to resolve whether the bacteria in the two species are monophyletic, or represent independent origins of male-killing among the B-group Wolbachia. W ...
... bipunctata, and the butterfly Acraea encedon. Both male-killing bacteria belong to the B group of Wolbachia. However, phylogenetic analyses were unable to resolve whether the bacteria in the two species are monophyletic, or represent independent origins of male-killing among the B-group Wolbachia. W ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.