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factor occupancy and gene expression Effects of
factor occupancy and gene expression Effects of

... differentially occupied sites were strongly enriched for heterozygous SNPs within 50 bp of the position of maximal ChIP signal (Fig. 2A), indicating that they may be the most functionally important nucleotides. We then compared the rate at which heterozygous SNPs occurred at motif versus non-motif i ...
factor occupancy and gene expression Effects of sequence variation
factor occupancy and gene expression Effects of sequence variation

... differentially occupied sites were strongly enriched for heterozygous SNPs within 50 bp of the position of maximal ChIP signal (Fig. 2A), indicating that they may be the most functionally important nucleotides. We then compared the rate at which heterozygous SNPs occurred at motif versus non-motif i ...
Comparative gene mapping in Arabidopsis lyrata chromosomes 6
Comparative gene mapping in Arabidopsis lyrata chromosomes 6

... intervals. We confirm the two rearrangements previously inferred (a reciprocal translocation and a large inversion, which we infer to be pericentric). By including markers around the centromere regions of A. thaliana chromosomes IV and V, we localize the AL6 centromere, and can localize the breakpoin ...
Genomic organisation of the Mal d 1 gene cluster on linkage group
Genomic organisation of the Mal d 1 gene cluster on linkage group

... pollen due to cross-reactivity between the major birch pollen allergen, Bet v 1, and the apple allergen Mal d 1 (Fritsch et al. 1998). The cross-reactivity of these allergens is due to their high amino acid sequence and structural similarity (Vanek-Krebitz et al. 1995). Bet v 1-like genes have also ...
Full text - PAHdb - McGill University
Full text - PAHdb - McGill University

... cDNA sequences have long been available [Kwok et al., 1985; Konecki et al., 1992] but the genomic sequence is recent (Konecki, D.S., unpublished; deposited in PAHdb Nov. 2001). The genomic sequence of the PAH gene and its flanking regions spans 171,266 bp with ~27 kbp of 50 untranslated region (50 U ...
Characterisation of the katA gene encoding a catalase and evidence
Characterisation of the katA gene encoding a catalase and evidence

... cassette (data not shown). In addition to the katA-containing band, a second weaker hybridisation signal of about 4.5 kb was detected in both strains, most likely due to a second catalase gene. Using other restriction enzymes, equivalent hybridisation patterns were obtained (data not shown). These r ...
Full Text  - Genes | Genomes | Genetics
Full Text - Genes | Genomes | Genetics

... stopped after quantitative trait locus mapping of traits of interest. One factor that has limited further genetic study of these traits is a limited set of genetic markers, which can facilitate fine-scale mapping. J. True and C. Laurie established a large collection of strains carrying P-element tran ...
A Novel CpG Island Set Identifies Tissue-Specific
A Novel CpG Island Set Identifies Tissue-Specific

The relationship between higher‑order chromatin structure and
The relationship between higher‑order chromatin structure and

... Tertiary levels of chromatin structure So far, we have focused on chromatin structure at the level of the 30 nm chromatin fibre, largely because this is the only level of higher‑order chromatin structure that we have reasonable models for. However, it is clear from electron microscopy studies that a ...
Linking Cognitive Neuroscience and Molecular Genetics: New Perspectives from Williams... Ursula Bellugi and Marie St. George (Eds.)
Linking Cognitive Neuroscience and Molecular Genetics: New Perspectives from Williams... Ursula Bellugi and Marie St. George (Eds.)

... breakpoints in the common WMS deletion, are thought to be located in these duplicated regions (Meng et al., 1998; Perez-Jurado et al., 1998; Osborne et al., 1997a; Korenberg et al., 1996; Robinson et al., 1996). However, the number of expressed genes in these duplicated regions and their contributio ...
Microsoft Word (Chapter 3) - DORAS
Microsoft Word (Chapter 3) - DORAS

... siderophores, haem and haemoglobin. After incubation for 24 – 48 hours at 30oC, halos of growth appear around wells where the test solutions are being utilised. Ferric chloride is generally added to one of the wells for each bioassay as a positive control. Strains with the ability to produce and uti ...
Single-stranded heteroduplex intermediates in l Red homologous
Single-stranded heteroduplex intermediates in l Red homologous

... recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined. Results: Here we show that the Red proteins can act via full length single stranded intermediates to establish single stranded heteroduplexes at the replication fork. We created asymmetrically dige ...
Mutualism and asexual reproduction influence recognition genes in a fungal... M.A.  van  der  Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*
Mutualism and asexual reproduction influence recognition genes in a fungal... M.A. van der Nest1, E.T. Steenkamp2, P.M.... Wingfield1 and B. Slippers1*

... (dS) substitution rate ratio (Yang and Nielson, 1998). To test for variation of selective pressures across the codons, goodness of fit was calculated for the different site-specific models proposed by Yang et al. (2000). Statistical significance was calculated with likelihood ratio tests (LRT), whic ...
Lecture 4
Lecture 4

... resource ...
Arsenite as an Electron Donor for Anoxygenic Photosynthesis
Arsenite as an Electron Donor for Anoxygenic Photosynthesis

... Both Mono Lake and Big Soda Lake waters contain abundant dissolved inorganic arsenic, 200 and 20 µM, respectively [5]. Mono Lake has been the more intensively studied of the two with respect to arsenotrophy [6] and has been found to support a breadth of arsenotrophic microbes. Arsenate-respiring bac ...
Distribution of orphan metabolic activities
Distribution of orphan metabolic activities

... activities are primarily clustered in less studied regions of the EC classification space. However, our results demonstrate that this is unlikely to be the case (Figure 2). Figure 2a presents the fractions of orphan activities at different levels of the EC classification organized in a tree hierarch ...
Localization and structural analysis of the ribosomal RNA operons of
Localization and structural analysis of the ribosomal RNA operons of

Relationships between a new type IV secretion system and the icm
Relationships between a new type IV secretion system and the icm

... both systems were shown to conjugate RSF1010-related plasmids between bacteria, using the icm/dot or vir gene products respectively. Second, the virulence of both systems was shown to be inhibited by an active RSF1010 conjugation system. Third, both systems contain proteins homologous to plasmid-enc ...
Detailed Algorithm of the DBRF
Detailed Algorithm of the DBRF

Guidelines for interpretation of 16S rRNA gene sequence
Guidelines for interpretation of 16S rRNA gene sequence

Annotation Strategy Guide - GEP Community Server
Annotation Strategy Guide - GEP Community Server

...  Selecting a better supported splice site: While extension of an open reading frame is important for conserving the length of the coding exon, it is also important to consider selecting the best-supported splice site. For example, let’s look at the first coding exon (1_2365_0) of Dyrk3 in D. mojave ...
Proceedings as -file
Proceedings as -file

... Chair: Hein van Haeringen, Dr.Van Haeringen Laboratorium B.V, Wageningen, The Netherlands ƒ Welcome ƒ Comparison test: Comments from Duty Laboratory South Africa; Comments from Computing Laboratory The Netherlands; Discussion ƒ ISAG panels: Do they work well? How many laboratories are using these pa ...
Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology

... SDS PAGE was subjected to MALDI MS. Since this peptide mass fingerprinting revealed that no protein homologous to ASR1 phytase has been deposited within the protein databases, the highly sensitive nanoflowelectrospray mass spectrometry technique was applied in order to obtain specific sequence infor ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria

Making Genetics Easy and Fun - California Science Teachers
Making Genetics Easy and Fun - California Science Teachers

... Reinforce mitosis and consider optional enrichment topics related to developmental biology, embryology, stem cell technology, etc Teach Mendelian genetics Fill out the Punnett square worksheet to predict traits of offspring Give birth: decorate baby bag with phenotypes that match its genotypes and f ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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