DNA cytosine methylation in plant development
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
Concordance trees, concordance factors, and the exploration of
... has a known population history. At best one has molecular data for a number of loci for a set of individuals representative of the taxa under study. If we are prepared to make some assumptions about the nature of populations and genetic processes, these data contain information on the history of the ...
... has a known population history. At best one has molecular data for a number of loci for a set of individuals representative of the taxa under study. If we are prepared to make some assumptions about the nature of populations and genetic processes, these data contain information on the history of the ...
FabXpress Antibody Production Kit Product Flyer.indd
... working routinely with phages. By repetitive culturing of TG1F+ bacteria on rich medium, clones that have lost the episome can be selected, as the maintenance of the F’ episome requires regular passaging on M9 minimal agar plates. Other bacterial strains can also be used, e.g. BL21, JM83 or TOP10. F ...
... working routinely with phages. By repetitive culturing of TG1F+ bacteria on rich medium, clones that have lost the episome can be selected, as the maintenance of the F’ episome requires regular passaging on M9 minimal agar plates. Other bacterial strains can also be used, e.g. BL21, JM83 or TOP10. F ...
Gene Detection Systems Catalog
... the particular changes to be made and the effect, if any, of such changes on the price and time of delivery. Buyer may not cancel this order unless such cancellation is expressly agreed to in writing by Seller. In such event, Seller will advise Buyer of the total charge for such cancellation, and Bu ...
... the particular changes to be made and the effect, if any, of such changes on the price and time of delivery. Buyer may not cancel this order unless such cancellation is expressly agreed to in writing by Seller. In such event, Seller will advise Buyer of the total charge for such cancellation, and Bu ...
Multiplex in-vitro Detection using SERS
... fluorescence emission bands, which possess large spectral overlaps, making multiplexing more difficult and also providing limited structural information. This is also the case for colorimetric detection where SERS offers greater sensitivity and an increased potential for multiplexed analysis.26 Cult ...
... fluorescence emission bands, which possess large spectral overlaps, making multiplexing more difficult and also providing limited structural information. This is also the case for colorimetric detection where SERS offers greater sensitivity and an increased potential for multiplexed analysis.26 Cult ...
PDF
... diverged. In an elegant study, Shaver et al (Shaver et al., 2010) identified the novel computational PRC2-homologs in several unicellular species and showed that E(z)-homolog in unicellular green algae Chlamydomonas reinhardtii is responsible for mono- and di-methylation of lysine 27 on histone H3. ...
... diverged. In an elegant study, Shaver et al (Shaver et al., 2010) identified the novel computational PRC2-homologs in several unicellular species and showed that E(z)-homolog in unicellular green algae Chlamydomonas reinhardtii is responsible for mono- and di-methylation of lysine 27 on histone H3. ...
Properties of Mitotic and Meiotic Recombination in the
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
... array, and heterozygous for markers flanking the array, we measured inter-homolog recombination and intra-/sister-chromatid exchange in the CUP1 locus. The rate of intra-/sister chromatid recombination exceeded the rate of inter-homolog recombination by more than ten-fold. Loss of the Rad51 and Rad5 ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... plasmid DNA mixture in closed circular form, single selection for Leu+ transformants (and also for Ura+ transformants, not shown) reveals a first order concentrationdependence, while double selection for Ura+ Leu+ transformants results in a second order dependence. These outcomes are expectedon the ...
... plasmid DNA mixture in closed circular form, single selection for Leu+ transformants (and also for Ura+ transformants, not shown) reveals a first order concentrationdependence, while double selection for Ura+ Leu+ transformants results in a second order dependence. These outcomes are expectedon the ...
Predicting tRNA and tmRNA genes Aragorn - SEA
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
Predicting_tRNA_and_tmRNA_genes_12-2-16
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
... literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the others within its genome found by the programs, or encoded on the opposite strand as all the others, or encoded within a ORF called by GeneMark or Glimmer that has high coding ...
Bacteriophage A cloning system for the construction of
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
... endonucleases at the cloning site, subsequent treatment with phosphatase to ensure high insertion rates would be unnecessary. (iv) DNA inserts can be cloned in a known orientation relative to vector-encoded expression signals. The cloning vector XORF8 possesses a multiple cloning site (MCS) containi ...
View PDF - G3: Genes | Genomes | Genetics
... The budding yeast Sc. cerevisiae and fission yeast Schizosaccahromyces (S.) pombe are inexpensive model eukaryotic organisms used for biological research. Notably, most research areas of yeast could not be investigated in a timely manner in higher eucaryotes. Because mechanisms of many biological pat ...
... The budding yeast Sc. cerevisiae and fission yeast Schizosaccahromyces (S.) pombe are inexpensive model eukaryotic organisms used for biological research. Notably, most research areas of yeast could not be investigated in a timely manner in higher eucaryotes. Because mechanisms of many biological pat ...
Are Incomplete Denitrification Pathways a Common Trait in Thermus
... 60 °C. The primary focus of this study was to investigate the denitrification pathways of Thermus (Bacteria) isolates from geothermal springs from Tengchong, China. This study tested the hypothesis that incomplete denitrification is a common characteristic of the genus Thermus, regardless of geograp ...
... 60 °C. The primary focus of this study was to investigate the denitrification pathways of Thermus (Bacteria) isolates from geothermal springs from Tengchong, China. This study tested the hypothesis that incomplete denitrification is a common characteristic of the genus Thermus, regardless of geograp ...
View Tutorial
... The ordering of amino acids is directly translated from the sequence of mRNA nucleotides, ultimately tracing back to the DNA sequence. The translation between codons, triplets of nucleotides, and individual amino acids is known as the genetic code. This translation is mediated by molecules in the ce ...
... The ordering of amino acids is directly translated from the sequence of mRNA nucleotides, ultimately tracing back to the DNA sequence. The translation between codons, triplets of nucleotides, and individual amino acids is known as the genetic code. This translation is mediated by molecules in the ce ...
A GFP-lacZ Bicistronic Reporter System for Promoter Analysis
... The process of gene regulation in a living organism is vitally important for its adaptation to changing conditions in the environment. In fact, the analysis of the complete genomes currently available reveals that a large amount of the genome encodes sequences related to gene regulation and transcri ...
... The process of gene regulation in a living organism is vitally important for its adaptation to changing conditions in the environment. In fact, the analysis of the complete genomes currently available reveals that a large amount of the genome encodes sequences related to gene regulation and transcri ...
Loss-of-function of a Rice Gibberellin Biosynthetic Gene, GA20
... DNA gel blot analysis was done by using the coding sequences of GA20ox-1 and GA20ox-2 as probes. For the analysis of feedback repression, total RNAs were extracted from the seedlings treated with ± 10−4 M GA3 or ± 10−6 M uniconazole (an inhibitor of GA biosynthesis) and the DNA gel blot analysis was ...
... DNA gel blot analysis was done by using the coding sequences of GA20ox-1 and GA20ox-2 as probes. For the analysis of feedback repression, total RNAs were extracted from the seedlings treated with ± 10−4 M GA3 or ± 10−6 M uniconazole (an inhibitor of GA biosynthesis) and the DNA gel blot analysis was ...
The Human Genome Project: Genetic Screening and the
... messages to cells. Because there are only four elements that comprise DNA, it is necessary for DNA to encode its detailed directions in very long sequences of base pairs.' 6 For example, it is estimated that a typical small gene contains at least 3,000 base pairs and that each human cell contains ro ...
... messages to cells. Because there are only four elements that comprise DNA, it is necessary for DNA to encode its detailed directions in very long sequences of base pairs.' 6 For example, it is estimated that a typical small gene contains at least 3,000 base pairs and that each human cell contains ro ...
Genomics Reveal Ancient Forms of Stanniocalcin in Amphioxus and
... receptor remains to be identified in any organism. Our data suggest that amphioxus STCa is most similar to the common ancestor of vertebrate STCs because it has an 11th cysteine necessary for dimerization, an N-glycosylation motif, although not the canonical one in vertebrate STCs, and similar gene ...
... receptor remains to be identified in any organism. Our data suggest that amphioxus STCa is most similar to the common ancestor of vertebrate STCs because it has an 11th cysteine necessary for dimerization, an N-glycosylation motif, although not the canonical one in vertebrate STCs, and similar gene ...
ADP Ribosylation Factor is an Essential Protein in Saccharomyces cerevisiae and is Encoded by Two Genes.
... in press. Based on the in vitro ADP-ribosylation assay (36), ARF has been purified from a number of tissues, including bovine brain, in which ARF represents as much as 1 to 2% of total cell protein. ARF protein is encoded by at least two genes in both cows (26, 39) and humans (3). The bovine ARFI ge ...
... in press. Based on the in vitro ADP-ribosylation assay (36), ARF has been purified from a number of tissues, including bovine brain, in which ARF represents as much as 1 to 2% of total cell protein. ARF protein is encoded by at least two genes in both cows (26, 39) and humans (3). The bovine ARFI ge ...
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic
... 1993; Lorenz et al. 1995; Wach 1996). The simplicity and utility of PCR-mediated gene replacement has led to its usage in other fungal model organisms as well (Kaur et al. 1997; Walther and Wendland 2008; Wendland et al. 2000). Due to the standardized design of the pRS series, a single pair of oligo ...
... 1993; Lorenz et al. 1995; Wach 1996). The simplicity and utility of PCR-mediated gene replacement has led to its usage in other fungal model organisms as well (Kaur et al. 1997; Walther and Wendland 2008; Wendland et al. 2000). Due to the standardized design of the pRS series, a single pair of oligo ...