Analysis and nucleotide sequence of an origin of DNA replication in
... (d) Identification of the region necessary for stable, autonomous replication in ,4cinetobacter calcoaceticus A deletion analysis of the DNA from A. calcoaceficus in pWH 1266 was done to defme sequences necessary for stable replication and maintenance. This was carried out either by deleting parts ...
... (d) Identification of the region necessary for stable, autonomous replication in ,4cinetobacter calcoaceticus A deletion analysis of the DNA from A. calcoaceficus in pWH 1266 was done to defme sequences necessary for stable replication and maintenance. This was carried out either by deleting parts ...
Recombination and clonal groupings within Helicobacter pylori from
... bacteria from different continents or that particular alleles are selected for in certain populations. However, a different segment of the vacA gene was found to have recombined freely in bacteria isolated from Canada and South Africa (Suerbaum et al ., 1998). The virulence-associated cagA gene enco ...
... bacteria from different continents or that particular alleles are selected for in certain populations. However, a different segment of the vacA gene was found to have recombined freely in bacteria isolated from Canada and South Africa (Suerbaum et al ., 1998). The virulence-associated cagA gene enco ...
Allele Mining Strategies: Principles and Utilisation for Blast
... An important goal of modern crop science is to use nucleotide sequence variation to improve crops. Variation can either be natural, from divergent populations, or induced through treatment with mutagens (Till et al., 2007a). Mutation, among the fundamental forces of development, is usually a sudden ...
... An important goal of modern crop science is to use nucleotide sequence variation to improve crops. Variation can either be natural, from divergent populations, or induced through treatment with mutagens (Till et al., 2007a). Mutation, among the fundamental forces of development, is usually a sudden ...
RECOMBINANT DNA TECHNOLOGY AND BIOTECHNOLOGY
... Conditions and requirements that influence Enzyme Activity One unit activity:- is defined as amount of enzyme required to digest 1µg of reference DNA in 60 minutes at 370C. Usually reference DNA is λ phage DNA. Buffer:- The most critical determinant of the enzyme activity is the ionic concentration ...
... Conditions and requirements that influence Enzyme Activity One unit activity:- is defined as amount of enzyme required to digest 1µg of reference DNA in 60 minutes at 370C. Usually reference DNA is λ phage DNA. Buffer:- The most critical determinant of the enzyme activity is the ionic concentration ...
Gene quantification using real-time quantitative PCR
... DNA copy number measurements are important in determining the extent of genomic imbalance that underlies most malignancies. There are numerous techniques available for measuring DNA copy number in tumors; each method has specific advantages and disadvantages. Chromosomal CGH can detect imbalances ac ...
... DNA copy number measurements are important in determining the extent of genomic imbalance that underlies most malignancies. There are numerous techniques available for measuring DNA copy number in tumors; each method has specific advantages and disadvantages. Chromosomal CGH can detect imbalances ac ...
Evolution of synonymous codon usage in metazoans Laurent Duret
... the size of populations that can be studied in laboratories, this experimental approach is limited to the identification of mutations with a strong phenotypic impact [1]. This problem is far from negligible; for example, 50% of gene knockouts in yeast have no detectable effect on the phenotype [2]. ...
... the size of populations that can be studied in laboratories, this experimental approach is limited to the identification of mutations with a strong phenotypic impact [1]. This problem is far from negligible; for example, 50% of gene knockouts in yeast have no detectable effect on the phenotype [2]. ...
The Large Loop Repair and Mismatch Repair Pathways
... 2). We determined the level of recombination and the frequency of loop mismatch repair in wild-type strains and in strains lacking the MMR pathway gene PMS1 or the LLR pathway gene RAD1. As described in Introduction, RAD1 functions specifically in LLR during meiosis, while PMS1 has been demonstrated ...
... 2). We determined the level of recombination and the frequency of loop mismatch repair in wild-type strains and in strains lacking the MMR pathway gene PMS1 or the LLR pathway gene RAD1. As described in Introduction, RAD1 functions specifically in LLR during meiosis, while PMS1 has been demonstrated ...
15.13 Spm elements influence gene expression
... 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a cointegrate 15.8 Nonreplicative transposition proceeds by breakage and reunion 15.9 TnA transposition requires transposase and resolvase 15.10 Transposition of Tn ...
... 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a cointegrate 15.8 Nonreplicative transposition proceeds by breakage and reunion 15.9 TnA transposition requires transposase and resolvase 15.10 Transposition of Tn ...
Figure 15.6 Nonreplicative transposition allows a transposon to
... orientation in the same molecule of DNA; they are not necessarily adjacent. Inverted terminal repeats are the short related or identical sequences present in reverse orientation at the ends of some transposons. IS is an abbreviation for insertion sequence Transposase is the enzyme activity involved ...
... orientation in the same molecule of DNA; they are not necessarily adjacent. Inverted terminal repeats are the short related or identical sequences present in reverse orientation at the ends of some transposons. IS is an abbreviation for insertion sequence Transposase is the enzyme activity involved ...
the Role of DNA Sequence Data - International Journal of
... divergence levels within and between populations, where each such expectation is the average divergence over all painvise comparisons among cells averaged over all time (14). Thus, the average divergence observed at any one time might be higher or lower than the expected value, depending on how rece ...
... divergence levels within and between populations, where each such expectation is the average divergence over all painvise comparisons among cells averaged over all time (14). Thus, the average divergence observed at any one time might be higher or lower than the expected value, depending on how rece ...
Interfering RNA
... nucleic acid-based methods or other means and the gene sequence was known. • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi se ...
... nucleic acid-based methods or other means and the gene sequence was known. • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi se ...
Teacher Guide - Science Take-Out
... production of cartilage cells (chondrocytes), resulting in bone growth. IGF also stimulates the production of myoblasts, the cells that form muscles. ...
... production of cartilage cells (chondrocytes), resulting in bone growth. IGF also stimulates the production of myoblasts, the cells that form muscles. ...
Cloning and Genetic Analysis of Six Pyrroloquinoline
... same mutation by using a single, large culture, as described in Methods. The numerous colonies isolated from this culture on succinate medium containing ally1 alcohol were re-isolated on the same medium. Five per cent retained their ability to grow on methanol medium. Among 1400 colonies unable to g ...
... same mutation by using a single, large culture, as described in Methods. The numerous colonies isolated from this culture on succinate medium containing ally1 alcohol were re-isolated on the same medium. Five per cent retained their ability to grow on methanol medium. Among 1400 colonies unable to g ...
Systematic Mutational Analysis of the Yeast ACT1 Gene.
... many potential binding and otherfunctional domains of the protein. Recently, powerful general techniques for systematic mutagenesis of protein coding sequenceshave beendeveloped. These were aimed in the firstinstance at in vitro biochemical studies of the proteins, for which it was desired to alter ...
... many potential binding and otherfunctional domains of the protein. Recently, powerful general techniques for systematic mutagenesis of protein coding sequenceshave beendeveloped. These were aimed in the firstinstance at in vitro biochemical studies of the proteins, for which it was desired to alter ...
Discovery and classification of ecological diversity in the
... divergence levels within and between populations, where each such expectation is the average divergence over all painvise comparisons among cells averaged over all time (14). Thus, the average divergence observed at any one time might be higher or lower than the expected value, depending on how rece ...
... divergence levels within and between populations, where each such expectation is the average divergence over all painvise comparisons among cells averaged over all time (14). Thus, the average divergence observed at any one time might be higher or lower than the expected value, depending on how rece ...
Evolutionary relationships of the Tas2r receptor gene families in
... partial sequences, two contained a frameshift each and 27 appeared to be full-length Tas2r genes. We aligned all known, publicly available, TAS2R receptor sequences and developed an HMM model characteristic of the TAS2R family to search in a database of protein translations of predicted mouse genes ...
... partial sequences, two contained a frameshift each and 27 appeared to be full-length Tas2r genes. We aligned all known, publicly available, TAS2R receptor sequences and developed an HMM model characteristic of the TAS2R family to search in a database of protein translations of predicted mouse genes ...
The Chloroplast trnT–trnF Region in the Seed Plant
... gymnosperm lineage Gnetales (Gnetum, Welwitschia, and Ephedra), with especially dense sampling in Gnetum for which we sequenced 41 accessions, representing most of the 25–35 species. The trnL intron has a conserved secondary structure and contains elements that are homologous across land plants, whi ...
... gymnosperm lineage Gnetales (Gnetum, Welwitschia, and Ephedra), with especially dense sampling in Gnetum for which we sequenced 41 accessions, representing most of the 25–35 species. The trnL intron has a conserved secondary structure and contains elements that are homologous across land plants, whi ...
Application of whole genome sequencing to fully characterise
... sequence (of strain NCTC11168) was published in 2000 (28), numerous other Campylobacter genomes have been sequenced, revealing extensive within-species diversity (29-31). Building on these comparative studies involving relatively small numbers of isolates, WGS analysis has since been used in genome- ...
... sequence (of strain NCTC11168) was published in 2000 (28), numerous other Campylobacter genomes have been sequenced, revealing extensive within-species diversity (29-31). Building on these comparative studies involving relatively small numbers of isolates, WGS analysis has since been used in genome- ...
Assaying … promoter activity
... In order to determine whether the transposome had really inserted at random into the genome, we examined the sequences in the genome into which the transposons had inserted. To do this, we first recovered each of the transposons along with a portion of the adjacent genomic DNA via a plasmid rescue p ...
... In order to determine whether the transposome had really inserted at random into the genome, we examined the sequences in the genome into which the transposons had inserted. To do this, we first recovered each of the transposons along with a portion of the adjacent genomic DNA via a plasmid rescue p ...
1: Summary and Options
... in human beings focus on physiological and biochemical effects of mutations because, until recently, it was not possible to examine changes in DNA directly. Each of the current methods detects only a limited portion of the spectrum of mutational changes. These methods have been used to derive estima ...
... in human beings focus on physiological and biochemical effects of mutations because, until recently, it was not possible to examine changes in DNA directly. Each of the current methods detects only a limited portion of the spectrum of mutational changes. These methods have been used to derive estima ...
Molecular characterization of dioxygenases from polycyclic aromatic
... These primer pairs were also used to detect the nidA and nidB homologues in total genomic DNA extracts of strains Mycobacterium sp. PAH 2.135 (RJGII-135), M. £avescens PYR-GCK, M. gilvum BB1 and M. frederiksbergense FAn9T, with M. vanbaalenii PYR-1 as a control. The concentration of the primers was ...
... These primer pairs were also used to detect the nidA and nidB homologues in total genomic DNA extracts of strains Mycobacterium sp. PAH 2.135 (RJGII-135), M. £avescens PYR-GCK, M. gilvum BB1 and M. frederiksbergense FAn9T, with M. vanbaalenii PYR-1 as a control. The concentration of the primers was ...
Genetically Essential and Nonessential a-Tubulin Genes Specify Functionally Interchangeable Proteins.
... copy of this gene. If the plasmid integrates by a single homologous recombination event at the locus of the gene of interest, the result is a disrupted gene which consists of two partial copies of the gene flanking the plasmid sequences containing the disruption marker. Two other common types of rec ...
... copy of this gene. If the plasmid integrates by a single homologous recombination event at the locus of the gene of interest, the result is a disrupted gene which consists of two partial copies of the gene flanking the plasmid sequences containing the disruption marker. Two other common types of rec ...
The msh2 Gene of Schizosaccharomyces pombe Is
... DNA-binding domain. Cytological analysis revealed that during meiotic prophase of msh2-defective cells, chromosomal structures were frequently formed; such structures are rarely found in the wild type. Our data show that besides having a function in mismatch repair, S. pombe msh2 is required for cor ...
... DNA-binding domain. Cytological analysis revealed that during meiotic prophase of msh2-defective cells, chromosomal structures were frequently formed; such structures are rarely found in the wild type. Our data show that besides having a function in mismatch repair, S. pombe msh2 is required for cor ...
Engineering a tRNA and aminoacyl-tRNA synthetase for the site
... required. This orthogonal synthetase must then be engineered to uniquely acylate the tRNA with the desired unnatural amino acid, but not with any other amino acid. A final requirement is that the unnatural amino acid must be transported from the growth medium to the cytoplasm (or be biosynthesized i ...
... required. This orthogonal synthetase must then be engineered to uniquely acylate the tRNA with the desired unnatural amino acid, but not with any other amino acid. A final requirement is that the unnatural amino acid must be transported from the growth medium to the cytoplasm (or be biosynthesized i ...
Document
... mRNA sequences of more than about 50 codons. All together they analyzed 90 sequences… ...
... mRNA sequences of more than about 50 codons. All together they analyzed 90 sequences… ...