• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Number 2 - Laboratory Animal Boards Study Group
Number 2 - Laboratory Animal Boards Study Group

... in the transforming growth factor alpha transgenic mice was found to be dependent on the genetic background as well. And the analysis of striatal dopamine of the HPRT deficient mice clearly demonstrated that the degree of dopamine loss was also dependent on the genetic background. Other variable fac ...
Replication of plasmids with the p15A origin in Shewanella
Replication of plasmids with the p15A origin in Shewanella

Scoring Guidelines - Ohio Assessment Systems
Scoring Guidelines - Ohio Assessment Systems

me-6 - Genetics
me-6 - Genetics

... site (the one closer to wc) as the one more frequently converted to prototrophy. However, the map can be divided into two regions around the point represented by the alleles NM2lt and "54. Crosses within the right-hand region, and most particularly, crosses of either NM2lt o r NM254 with alleles to ...
Pairing of homologous regions in the mouse genome is associated
Pairing of homologous regions in the mouse genome is associated

... fragments is located in the central imprinting control region (KvDMR) and was chosen as bait, rendering the 4C interaction profiles allele-specific. As anticipated, the vast majority of interactions occurs on the cis allele but strikingly, we also found a number of chimeric 4C products consisting of ...
PPTX - Bioinformatics.ca
PPTX - Bioinformatics.ca

Complete Laboratory PDF
Complete Laboratory PDF

... Since Alfred Sturtevant constructed the first genetic map of a Drosophila chromosome in 1913, new mutations have been mapped using his method of linkage analysis. Determining the map position of a new mutation – and its corresponding gene – consists of testing for linkage with a number of previously ...
Microsporogenesis in Maize`
Microsporogenesis in Maize`

... for 3 to 4 h on a metal tray. They were then thawed at room temperature while shaking at 30 rpm. The buffer was replaced with 20 ml of staining solution containing 86 mm Na phosphate (pH 7.3), 0.3 M p-nitroblue-tetrazolium chloride (Sigma Chemical Co.), 1.0 mM NAD+ (ICN Nutritional Biochemicals), an ...
Chemical Genetics
Chemical Genetics

... nuclei in the conidia and the vegetative mycelium. This means that there are no problems of dominance (Chapter 3); there is only one dose of each gene in any nucleus and any mutant characteristic will be expressed unless the genetic background contains special modifier or suppressor genes. It is the ...
Phytic Acid and Inorganic Phosphate Composition in Soybean Lines
Phytic Acid and Inorganic Phosphate Composition in Soybean Lines

... Based on in vitro experiments with maize and potato IPK1 recombinant enzymes and work in Arabidopsis, the plant IPK1 gene appears to have the capacity to encode an inositol polyphosphate kinase, with lower inositol phosphates also capable of being substrates in addition to inositol-pentakisphosphate ...
Caenorhabditis elegans chromosome arms are anchored to the
Caenorhabditis elegans chromosome arms are anchored to the

Laboratory Report Guidelines and Rubric
Laboratory Report Guidelines and Rubric

... were done, an overview of the general methods used, and the main outcome. Although abstracts are short, they are probably the hardest of all sections to write. Introduction The introduction needs to include the following background information: Arabidopsis as a model genetic system, basic leaf devel ...
PDF - American Society of Mammalogists
PDF - American Society of Mammalogists

... silence of the same X allele in descendant cells is an example of epigenetics. Epigenetic effects are those in which heritable changes in gene activity are produced without a change in DNA sequence. Although epigenetic effects are typically thought of as occurring within an organism, they also may i ...
Accepted Version - CSIRO Research Publications Repository
Accepted Version - CSIRO Research Publications Repository

... * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel./Fax: +81-25-262-6615. Received: 30 May 2012; in revised form: 27 July 2012 / Accepted: 30 July 2012 / Published: 8 August 2012 ...
Structural Variants in the Human Genome
Structural Variants in the Human Genome

... Copy-paste mechanism to insert elsewhere ...
MOLECULAR PROFILING OF RICE (Oryza sativa L
MOLECULAR PROFILING OF RICE (Oryza sativa L

... in a mapping population it is possible to identify the putative candidate genes associated with the marker sequence. Thus study of quantitative ...
Life 9e - Garvness
Life 9e - Garvness

... 43. People with sickle-cell disease have a(n) _______ abnormality. a. phenylalanine hydrolyase b. oncogene c. cholesterol transport d. hemoglobin e. None of the above Answer: d Textbook Reference: 15.3 How Do Defective Proteins Lead to Diseases? Page: 328 Bloom’s Category: 1. Remembering 44. Sickle- ...
A program for annotating and predicting the effects of single
A program for annotating and predicting the effects of single

... produce a protein with 57 additional N-terminal amino acids compared with the reference gene (Fig. 2B). However, the three bases prior to the new 5'-ATG-3' sequence, 5'-AAT-3', is a poor match to the Kozak consensus sequence, 5'-ACC-3', discussed above in reference 12. Therefore, it is unclear wheth ...
pdf
pdf

... Let’s think about the general recombination shown in Fig. 8.1 in this context. The two chromosomes outlined in the figure are in a heterozygous parent, with the wild type alleles for genes A and B (A+ and B+) are on one chromosome and the mutant alleles (A- and B-) are on the homologous chromosome ( ...
Increased transversions in a novel mutator colon cancer cell line
Increased transversions in a novel mutator colon cancer cell line

... (6TG) resistant mutants were initially characterized by hprt RT ± PCR and cDNA sequencing (Table 1). Base substitutions are the predominant type of mutation recovered (70% of total mutations). Transversions (50% of total mutations and 71% of base substitutions) occur more than twice as frequently as ...
Research Project Final Report
Research Project Final Report

... mutant pea populations, enabling the isolation of genes known only by their phenotypic effect together with the identification of genetic variants in selected target traits. All of these activities have progressed along with the refinement of genetic maps and the generation of molecular markers. We ...
Repeated Sequences in CASPASE-5 and FANCD2 but not NF1 Are
Repeated Sequences in CASPASE-5 and FANCD2 but not NF1 Are

... DNA replication errors. As expected, MMR deficiency is associated with an increased mutation rate and with cancer (for review, see ref. 1). A significant fraction of certain tumors— notably, but not exclusively, those of endometrial, colorectal, and other gastrointestinal sites—are MMR defective. In ...
cbb752-mg-spr09-bioinfo
cbb752-mg-spr09-bioinfo

... • One idea for a definition? Bioinformatics is conceptualizing biology in terms of molecules (in the sense of physical-chemistry) and then applying “informatics” techniques (derived from disciplines such as applied math, CS, and statistics) to understand and organize the information associated with ...
pTransgenesis: a cross-species, modular
pTransgenesis: a cross-species, modular

... four elements, including sequences that facilitate transgene genome integration, a selectable marker and promoter elements driving a coding gene. Linking these four elements in a DNA construction, however, can be a rate-limiting step in the design and creation of transgenic organisms. In order to ex ...
The Acquisition of Student Nurses` Knowledge of Genetics
The Acquisition of Student Nurses` Knowledge of Genetics

... genomic healthcare through their emphasis of health promotion, prevention, screening, caring, and patient, family, and community relationships. Nurses are expected to have genetic and genomic knowledge that can be integrated into clinical practice. However, researchers today are finding nursing stud ...
< 1 ... 26 27 28 29 30 31 32 33 34 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report