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Extensive tRNA gene changes in synthetic Brassica
Extensive tRNA gene changes in synthetic Brassica

... A total of 1157 putative tRNA genes were predicted from B. rapa genome sequences using tRNA predicting program “tRNAscan-SE”. These genes were classified into 23 groups according to the type of tRNA isoacceptor (see Fig. 1 for detailed information). There were 67 intron-containing tRNA genes, belon ...
The Effects of Deleterious Mutations on Evolution at
The Effects of Deleterious Mutations on Evolution at

... estimating U has been to measure a fitness-related trait such as viability or fertility, in experiments where lines started from the same initial isogenic stock have fixed mutations independently, an approach pioneered in Drosophila by Terumi Mukai (Mukai 1964; Mukai et al. 1972). Selection against mu ...
Release Notes for Genomes Processed Using Complete Genomics
Release Notes for Genomes Processed Using Complete Genomics

... Results of CNV analysis for non-tumor and tumor genomes are reported in two files. They can be found in the CNV sub-directory of the ASM directory. i. cnvSegmentsBeta-[ASM-ID].tsv file reports segmentation of the complete reference genome into regions of distinct ploidy levels, giving the estimated ...
- California State University
- California State University

... consists of a bioinformatics analysis of the Denisovan genome with special focus on specific subsets of protein-coding genes that are functional in modern humans and that include at least one amino acid change between Denisovans and modern humans. The present study focuses primarily on single nucleo ...
Association between IGF1R / i16 / TaqI and IGF1 / SnaBI
Association between IGF1R / i16 / TaqI and IGF1 / SnaBI

... (determined as IGF1R / i16 / TaqI) three distinct patterns (designed as AA, AB, and BB genotypes) were observed in PCR-RFLP analysis. Digestion of the 878-bp PCR product with the restriction enzyme TaqI resulted in two DNA bands (545 and 333 bp) for homozygote AA and three (387, 333 and 158 bp) for ...
Supporting Information Legends
Supporting Information Legends

... marker positions. The dashed line in the middle row map indicates the unsequenced genomic region between the BAC clones AL606656 and AP009057 in the RAP-DB build 5. The AP009057 sequence containing the telomere was highly rich in repeat sequences. Recombinants with breakpoints beyond the SNP6 marker ...
MERE1, a Low-Copy-Number Copia-Type
MERE1, a Low-Copy-Number Copia-Type

... These copies are highly related, but only three of them have a complete coding region and polymorphism exists between the long terminal repeats of these different copies. This retroelement family is present in all M. truncatula ecotypes tested but also in other legume species like Lotus japonicus. I ...
Scientist finds the gene that determines major sensitivity to bitter taste
Scientist finds the gene that determines major sensitivity to bitter taste

... exclaimed how bitter the powder tasted. Fox was surprised -- he had been much closer to the chemical, but he tasted nothing at all. Both men tasted the chemical again. Again, Fox said the chemical was tasteless, but his co-worker insisted it was very bitter. Fox handed out crystals of the chemical, ...
Supplementary Figures (doc 9746K)
Supplementary Figures (doc 9746K)

... FGFR2 are shown. From right to left, they are exon 7, exon 8, exon 9, and exon 10. Exon 8 is the IIIb-specific exon, whereas exon 9 is IIIc-specific. The blue exon peaks are the alignment results of normal RNA-Seq reads, while the yellow ones are from tumor reads. The arcs with numbers in them show ...
Introduction The non-vascular, multicellular land plant
Introduction The non-vascular, multicellular land plant

... transformation of a transgenic Physcomitrella line harboring a plasmid containing a resistance marker gene with a second plasmid containing a different selection marker gene. Co-segregation analysis and molecular analysis confirmed that the second plasmid was integrated at the site of insertion of t ...
Databases_what_and_w..
Databases_what_and_w..

... Phenotypes Intra and Inter-species relationships • Phenotypes come from the proteins. Niche exploitation Ecosystems • Proteins come from the DNA via RNA. • Changes in DNA cause changes in proteins. • Changes in proteins cause changes in phenotypes. ...
Somatic Cell Gene Mutations in Humans
Somatic Cell Gene Mutations in Humans

... each measuring changes in a different "recorder" gene, are available for detecting mutations of the hemoglobin (Hb) and glycophorin A (gpa) genes in red blood cells and the hypoxanthine-guanine phosphoribosyltransferase (hprt) and HLA genes in T-lymphocytes. Mean adult background mutant frequencies ...
Inference of homologous recombination in bacteria using whole
Inference of homologous recombination in bacteria using whole

... and rate ρ in the corresponding graph, given the tree, the mutation rate θ and the recombination tract length δ. We consider sequences of length 10000bp, which is characteristic of genomic alignment block sizes. Inference on an ARG with N=10 sequences, θ = 300, ρ = 50 and δ = 236 is considered on F ...
MS26/CYP704B is required for anther and pollen wall
MS26/CYP704B is required for anther and pollen wall

... CYP704B1 catalyzes the in-chain and ω-hydroxylation of fatty acids and is essential for exine biosynthesis [14]. Pollen from cyp704b1 mutant plants lack normal exine, but remain viable and capable of fertilization [14]. The rice OsCYP704B2 gene encodes a long-chain fatty acid hydroxylase capable of ...
FEMS Microbiology Letters
FEMS Microbiology Letters

... the tetrameric and dimeric oxidoreductase-containing species do not possess a homodimeric PFO. Campylobacter jejuni is an exception as it contains both PFO and a tetrameric oxidoreductase (KOR, Fig. 1). The amino acid sequences corresponding to residues 1^398 in the Synechocystis 6803 PFO are su¤cie ...
CS790 – Introduction to Bioinformatics
CS790 – Introduction to Bioinformatics

... GCG-TIntro to Bioinformatics – Sequence Alignment ...
Slides
Slides

... Mapping Structural Variation in Humans >1 kb segments - Structural Variations are Common 40% of the genome -Structural Variations are involved in phenotype variation and disease - Until recently most methods for detection were low resolution (>50 kb) ...
PDF
PDF

... where TP, TN, FP and FN denote number of true positives, true negatives, false positives and false negatives, respecexons with 17% false positives. tively. We find that the accuracy of FirstEF is significantly higher for CpG-related than non-CpG-related genes. To study the performance of FirstEF on ...
Microarrays - Consortium for Mathematics and its Applications
Microarrays - Consortium for Mathematics and its Applications

... appointment for Ralph on the next available date. The dermatologist confirms your suspicion of melanoma with a biopsy of the mole and classifies it as stage II because the mole is more than 2mm thick but has not spread to the lymph nodes. The dermatologist informs you that he has ordered a microarra ...
Genetically modified soybean
Genetically modified soybean

... University’s Department of Molecular Biology, the “marker genes typically confer resistance to a selective agent, often an antibiotic,” so the unmodified cells can easily be killed off to leave only modified cells behind, and the “other [gene] is meant to confer a desirable phenotype, which is often ag ...
Leukaemia Section t(2;3)(p21;q26) THADA/MECOM Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(2;3)(p21;q26) THADA/MECOM Atlas of Genetics and Cytogenetics in Oncology and Haematology

... have been found in thyroid adenomas; it would compete with normal THADA, thereby disturbing normal apoptosis of follicular cells (Rippe et al., 2003; Kloth et al., 2011). ...
Marwa Yahia Ahmed_o
Marwa Yahia Ahmed_o

... It is becoming increasingly clear, as the DNA sequences of the human genome are being revealed, that many genes are polymorphic i.e., variable in DNA sequence. In coding or non-coding regions of a specific gene, there may be either a single base pair substitution of one nucleotide for another; figur ...
Activation of Silent Genes by Transposons Tn5 and TnlO
Activation of Silent Genes by Transposons Tn5 and TnlO

... vided by the insertion itself (BERG,WEISSand CROSSLAND 1980; BLAZEY and BURNS1982; CIAMPI,SCHMID and ROTH 1982; CIAMPIand ROTH 1988). Evidence that transposon T n 10 possesses an outward promoter has beenpresented by SIMONSet al. (1983);gene activation by the ISSOL element of T n 5 has been observed ...
巴西橡胶Pto类抗病同源序列的克隆与系统发育重建
巴西橡胶Pto类抗病同源序列的克隆与系统发育重建

... the activation domain between subdomains VII and VIII, and its internal P+1 loop site, which is responsible for the specific binding of AvrPto (Frederick et al, 1998), and also several invariant amino acids distributed throughout the sequences. In addition, three of the four autophosphorylation site ...
- Lab Fabiana Perocchi
- Lab Fabiana Perocchi

... negligible. Hybridizations of genomic DNA yielded estimates of relative affinities by providing a nominally uniform concentration along the genome (David et al, 2006). Allele expression levels and probe affinities in our non-linear, heteroscedastic model were inferred using iterative weighted least ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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