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... Chromosomal recombination has both beneficial and deleterious consequences. During meiosis, recombination is generally considered to be essential to the orderly distribution of chromosomes. In mitotically growing cells of lower organisms, recombination provides for efficient repair of DNA damage, pa ...
... Chromosomal recombination has both beneficial and deleterious consequences. During meiosis, recombination is generally considered to be essential to the orderly distribution of chromosomes. In mitotically growing cells of lower organisms, recombination provides for efficient repair of DNA damage, pa ...
An rpoB signature sequence provides unique resolution for the
... inclusion of residues that were discarded at the previous level of analysis because of automatic gap removal. The procedure was repeated on each new well-supported subgroup (bootstrap value .50 %) until all the sequences realigned possessed exactly the same signature sequence and thus maximal resolu ...
... inclusion of residues that were discarded at the previous level of analysis because of automatic gap removal. The procedure was repeated on each new well-supported subgroup (bootstrap value .50 %) until all the sequences realigned possessed exactly the same signature sequence and thus maximal resolu ...
Pourcel et al., Microbiology 2005
... The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or ‘spacers’. CRISPRs are present in both archaea and bacteria, in association with genes involved in DNA recombination and repair ...
... The remarkable repetitive elements called CRISPRs (clustered regularly interspaced short palindromic repeats) consist of repeats interspaced with non-repetitive elements or ‘spacers’. CRISPRs are present in both archaea and bacteria, in association with genes involved in DNA recombination and repair ...
Distortion of quantitative genomic and expression
... genomic DNA, in the presence and absence of Cot-1 DNA. Reactions were hybridized, washed, stained with SPE and then analyzed by flow cytometry. Quantitative PCR QPCR and data analysis were performed using the Chromo4 QPCR system (BioRad Laboratories, Hercules, CA). Primers and amplified intervals we ...
... genomic DNA, in the presence and absence of Cot-1 DNA. Reactions were hybridized, washed, stained with SPE and then analyzed by flow cytometry. Quantitative PCR QPCR and data analysis were performed using the Chromo4 QPCR system (BioRad Laboratories, Hercules, CA). Primers and amplified intervals we ...
Transgene inheritance in plants
... Agrobacterium-mediated transformation usually produces transgenic plants with a low copy number and the transgenes are transmitted to progeny according to Mendelian (HORSCH et al. 1984, BUDAR et al. 1986) and in some cases non-Mendelian inheritance (DEROLES, GARDNER 1988). The characteristic feature ...
... Agrobacterium-mediated transformation usually produces transgenic plants with a low copy number and the transgenes are transmitted to progeny according to Mendelian (HORSCH et al. 1984, BUDAR et al. 1986) and in some cases non-Mendelian inheritance (DEROLES, GARDNER 1988). The characteristic feature ...
Brand, Veronica - Degenerate Primer Design using Computational Tools
... such: “Given a training set of known genes, design a pair of primers, one for the 5’ side and another for the 3’ side, so that the primers would amplify many of the genes and would have degeneracy that does not exceed a predefined limit” (2005). A degenerate primer in this case is a primer where mul ...
... such: “Given a training set of known genes, design a pair of primers, one for the 5’ side and another for the 3’ side, so that the primers would amplify many of the genes and would have degeneracy that does not exceed a predefined limit” (2005). A degenerate primer in this case is a primer where mul ...
Pierre MADL Div. of Material Sciences Dep
... and his (largely wrong) theory of Germ Plasm, he is also widely quoted as having 'proved' the non-existence of Lamarckian inheritance by the experiment of chopping of the tails of fifteen hundred rats, repeatedly over 20 generations, and reporting that no rat was ever born in consequence without a t ...
... and his (largely wrong) theory of Germ Plasm, he is also widely quoted as having 'proved' the non-existence of Lamarckian inheritance by the experiment of chopping of the tails of fifteen hundred rats, repeatedly over 20 generations, and reporting that no rat was ever born in consequence without a t ...
Biosynthesis of the Antibiotic Nonribosomal Peptide Penicillin in
... The baker’s yeast Saccharomyces cerevisiae is the most well-characterised unicellular fungi and is also one of the main organisms used industrially for engineered biosynthesis4. Since the advent of synthetic biology, S. cerevisiae has been genetically reprogrammed with diverse enzymes from bacteria ...
... The baker’s yeast Saccharomyces cerevisiae is the most well-characterised unicellular fungi and is also one of the main organisms used industrially for engineered biosynthesis4. Since the advent of synthetic biology, S. cerevisiae has been genetically reprogrammed with diverse enzymes from bacteria ...
Mutations
... The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. Mutations are often thought o ...
... The effects of mutations on genes vary widely. Some have little or no effect; and some produce beneficial variations. Some negatively disrupt gene function. Whether a mutation is negative or beneficial depends on how its DNA changes relative to the organism’s situation. Mutations are often thought o ...
Genome-wide identification and analysis of the SGR
... Structure analysis of the melon SGR genes CmSGR ORF lengths ranged from 504 (CmSGR4) to 774 bp (CmSGR2), molecular weights ranged from 19.02 (CmSGR4) to 29.48 kDa (CmSGR2), and pI values ranged from 6.19 (CmSGR4) to 8.72 (CmSGR2) (Table 1). CmSGR genes were distributed on all chromosome of the melon ...
... Structure analysis of the melon SGR genes CmSGR ORF lengths ranged from 504 (CmSGR4) to 774 bp (CmSGR2), molecular weights ranged from 19.02 (CmSGR4) to 29.48 kDa (CmSGR2), and pI values ranged from 6.19 (CmSGR4) to 8.72 (CmSGR2) (Table 1). CmSGR genes were distributed on all chromosome of the melon ...
Introduction to BLAST ppt
... Topics for this Tutorial Review high-performance methods in computational genomics that belong one of the following classes ...
... Topics for this Tutorial Review high-performance methods in computational genomics that belong one of the following classes ...
Genetic association between the PRKCH gene encoding protein
... DRB1 alleles encode a conserved amino acid sequence (QKRAA, QRRAA, or RRRAA) in the third hypervariable region of the molecule, which is commonly called the shared epitope (5). The development of severe disease has also been associated with the HLA– DRB1 shared epitope (6). However, it is estimated ...
... DRB1 alleles encode a conserved amino acid sequence (QKRAA, QRRAA, or RRRAA) in the third hypervariable region of the molecule, which is commonly called the shared epitope (5). The development of severe disease has also been associated with the HLA– DRB1 shared epitope (6). However, it is estimated ...
Chromosomal breakpoint positions suggest a direct role for radiation
... chromosomal regions with increased lability (fragile) sites (Ehrlich et al., 1993; Stary and Sarasin, 1992; Hyrien et al., 1987). By contrast, our results indicate that in post-Chernobyl tumors the RET/PTC breakpoints were distributed relatively randomly across the respective introns, except for clu ...
... chromosomal regions with increased lability (fragile) sites (Ehrlich et al., 1993; Stary and Sarasin, 1992; Hyrien et al., 1987). By contrast, our results indicate that in post-Chernobyl tumors the RET/PTC breakpoints were distributed relatively randomly across the respective introns, except for clu ...
Defining the Epigenetic Mechanism of Asymmetric Cell Division of
... named) Watson strand-containing chromatid is switching competent, and its sister cell, inheriting the template Crick strand-containing chromatid, is always incompetent (Klar 1987a, 1990, 2007). The difference in strands is the result of the DNA sequence- and strand-specific imprint moiety installed a ...
... named) Watson strand-containing chromatid is switching competent, and its sister cell, inheriting the template Crick strand-containing chromatid, is always incompetent (Klar 1987a, 1990, 2007). The difference in strands is the result of the DNA sequence- and strand-specific imprint moiety installed a ...
Extrapolation to the whole human genome
... pseudogenes based on whether there is a continuous span of homology that is >70% of the length of the closest matching human protein (i.e. with introns removed), or whether there is evidence of polyadenylation. We have applied our approach to chromosomes 21 and 22, the first parts of the human genom ...
... pseudogenes based on whether there is a continuous span of homology that is >70% of the length of the closest matching human protein (i.e. with introns removed), or whether there is evidence of polyadenylation. We have applied our approach to chromosomes 21 and 22, the first parts of the human genom ...
Definitions for annotating CDS sequences
... In the final clone, if the coding sequence of the gene of interest can be transferred away from its STOP codon through simple molecular biological methods (e.g., universal restriction site(s), recombination reactions, Gateway, etc.), thus allowing different carboxyl terminal tags to be appended to t ...
... In the final clone, if the coding sequence of the gene of interest can be transferred away from its STOP codon through simple molecular biological methods (e.g., universal restriction site(s), recombination reactions, Gateway, etc.), thus allowing different carboxyl terminal tags to be appended to t ...
Ehlers-Danlos syndrome type VIIA and VIIB result from splice
... VIIC). We identified seven additional families in which EDS type VII is either dominantly inherited (one family with EDS type VIIB) or due to new dominant mutations (one family with EDS type VIIA and five families with EDS type VIIB). In six families, the mutations alter the consensus splice junctio ...
... VIIC). We identified seven additional families in which EDS type VII is either dominantly inherited (one family with EDS type VIIB) or due to new dominant mutations (one family with EDS type VIIA and five families with EDS type VIIB). In six families, the mutations alter the consensus splice junctio ...
Physical map of the aromatic amine and m-toluate
... A restriction endonuclease map was derived for the aromatic amine and m-toluate catabolic plasmid pTDNl present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-pr ...
... A restriction endonuclease map was derived for the aromatic amine and m-toluate catabolic plasmid pTDNl present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-pr ...
Methods for pattern discovery in unaligned biological sequences
... in the sequences, and another one generating the instances of the real motif, according to two separate probability distributions. The goal of the algorithm is to build a model for the source generating the pattern. Then, for each input sequence, the substring that best ®ts the model is considered t ...
... in the sequences, and another one generating the instances of the real motif, according to two separate probability distributions. The goal of the algorithm is to build a model for the source generating the pattern. Then, for each input sequence, the substring that best ®ts the model is considered t ...
Biotechnology Explorer™ Ligation and Transformation - Bio-Rad
... survive. For example, resistance to the antibiotic ampicillin is conveyed by a plasmid carrying an ampicillin-resistance gene. Plasmids are capable of being transferred from one bacterium to another. These characteristics have resulted both in wonderful new uses for plasmids (such as their use in cl ...
... survive. For example, resistance to the antibiotic ampicillin is conveyed by a plasmid carrying an ampicillin-resistance gene. Plasmids are capable of being transferred from one bacterium to another. These characteristics have resulted both in wonderful new uses for plasmids (such as their use in cl ...
Full-Text PDF
... δ-carotene (ε,ψ-carotene) to produce α-carotene. No ε-cyclization activity was identified for BfLCYB. Sequence comparison showed that BfLCYB shares conserved domains with other functionally characterized lycopene cyclases from different organisms and belongs to a group of ancient lycopene cyclases. ...
... δ-carotene (ε,ψ-carotene) to produce α-carotene. No ε-cyclization activity was identified for BfLCYB. Sequence comparison showed that BfLCYB shares conserved domains with other functionally characterized lycopene cyclases from different organisms and belongs to a group of ancient lycopene cyclases. ...
Genomic Sequence Data - G3: Genes | Genomes | Genetics
... generally isolated from diseased tissue samples and thus are found as subsets of complex metagenomic data that also includes host sequence and, commonly, non-pathogenic commensal microflora. Viral DNA or RNA typically makes up only a tiny fraction of the total nucleic acid in such samples, and while ...
... generally isolated from diseased tissue samples and thus are found as subsets of complex metagenomic data that also includes host sequence and, commonly, non-pathogenic commensal microflora. Viral DNA or RNA typically makes up only a tiny fraction of the total nucleic acid in such samples, and while ...