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Castric et al. (MBE 2010) - GEPV
Castric et al. (MBE 2010) - GEPV

... effective migration rate (i.e., migration rate taking into account the probability of successful introduction, which depends on the allele’s dominance level). More specifically, dominant S-alleles could be present in a larger number of demes than recessive S-alleles if their effective migration rate ...
Genetics of Bacteriophage P22. II. Gene Order and Gene Function.
Genetics of Bacteriophage P22. II. Gene Order and Gene Function.

... Fie. 1. The genetic m&p of phage P22 drawn approximately to scale. The genetic size of each gene is indicated; this was determined by the largest frequency of recombination between mutations in a complementation group. Also shown are frequencies of recombination between particular mutants in the var ...
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY
KARNATAKA COMMON ENTRANCE TEST – MAY, 2016 BIOLOGY

... Disclaimer: These solutions are provided by the academic team of BASE. These solutions may be different from those to be provided by the Karnataka Examinations Authority (KEA) due to difference in assumptions taken in support of the solutions. In such cases solution provided by KEA will be deemed as ...
BBSI Final Report
BBSI Final Report

... aureus; all carrying genes related to the virulence of the species. Mobility has been demonstrated for SaPI1, the first PI identified in S. aureus, and this transfer requires the helper bacteriophage 80. SaPI is transferred to other cells via transducing particles that are 1/3 the size of the norma ...
View/Open
View/Open

... Sources of genetic variation Genetic variation for a trait in a population can either exist naturally or induce artificially through mutation. Therefore, both natural and induced variations are important for studying genetics of a trait and making genetic improvement in crop plants. In a natural pop ...
24 Recombination Hotspots in Nonallelic Homologous Recombination
24 Recombination Hotspots in Nonallelic Homologous Recombination

... rates can be several orders of magnitude more than in surrounding “cold” regions. In parallel to these developments, sufficient numbers of breakpoints of selected NAHR rearrangements have been characterized at the DNA sequence level to resolve the distribution of crossovers in these cases. This has ...
Fumarase Deficiency
Fumarase Deficiency

... analysis and prenatal diagnosis of human fumarase deficiency. Molecular genetics and metabolism,63(4), 254-262. Pereira de Padua, R. A., & Nonato, M. C. (2014). Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of recombinant human fumarase. Acta Crystallo ...
Introduction to Molecular Diagnostics
Introduction to Molecular Diagnostics

Chapter 1 - Institut Montefiore
Chapter 1 - Institut Montefiore

... Reading the code • Because there are only 20 amino acids that need to be coded, the genetic code can be said to be degenerate, with the third position often being redundant • Depending on the starting point of reading, there are three possible variants to translate a given base sequence into an amin ...
A genetic linkage map for watermelon based on
A genetic linkage map for watermelon based on

... between PI 296341-FR and NHM (based on all fragments produced by 120 primers) is 2 × 285/(563 + 608) = 48.6%. Thus, a high level of polymorphism is expected from the present cross. MAP CONSTRUCTION AND MARKER SEGREGATION. One hundred and fifty-six markers (87.1%) of the 179 markers analyzed could be ...
Prediction and investigation of novel proteins in DNA double
Prediction and investigation of novel proteins in DNA double

... DNA double stranded breaks (DSBs) are the most genotoxic forms of DNA lesions, causing fragmentation of the DNA strands. Mis-repaired and unrepaired DSBs lead to chromosomal rearrangement and genomic instability promoting tumorigenesis or cell death. DSBs are primarily repaired by two independent an ...
Structural and Functional Studies of Insertion Element IS200
Structural and Functional Studies of Insertion Element IS200

... The nucleotide sequence of the insertion element 15200 has been determined partially, including the junctions between the element and the host chromosome at the insertion site, At most, two bases (A-A) are found repeated at the junctions and could be duplications of host sequences generated by the i ...
PPTX - Bioinformatics.ca
PPTX - Bioinformatics.ca

... Using MetaPhlan • MetaPhlan uses Bowtie2 for sequence similarity searching (nucleotide sequences vs. nucleotide database) • Paired-end data can be used directly • Each sample is processed individually and then multiple sample can be combined together at the last step • Output is relative abundances ...
functional analysis of chromatin assembly genes in tetrahymena
functional analysis of chromatin assembly genes in tetrahymena

... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
Complete genome sequence of the thermophilic Thermus sp
Complete genome sequence of the thermophilic Thermus sp

... of synteny. The three closest Thermus with sequenced genomes (T. thermophilus strains HB27, HB8 and T. scotoductus SA-01) were selected for the comparison. The genome of strain HB27 consists of a chromosome (1.89 Mb) and a megaplasmid (0.23 Mb). On the other hand, strain HB8 has a chromosome of 1.85 ...
TEL Gene Is Involved in Myelodysplastic Syndromes
TEL Gene Is Involved in Myelodysplastic Syndromes

... A 1,241-bp TEL cDNA was obtained containing the complete coding sequence. The sequence of this cDNA was identical to the published one.I3 The cDNA was used to screen a chromosome 12 cosmid library. Ten cosmids were obtained and characterized by Southern hybridization with restriction fragments of th ...
SAB-2010
SAB-2010

... Based on the SAB suggestions, the progress has been made towards the goal of extending the annotation of pathway databases in Cyc and Wiki versions in an automated way. However to do that approach we have to streamline the data workflow and structure the current curated gene database as a central re ...
Uria_et_al_2006 _ADH paper
Uria_et_al_2006 _ADH paper

... reduced application of organic solvents (Julich Chiral Solutions-Germany, http://www.julich.com/). In addition, enzymes (including ADH) can be immobilized and reused for many enzymatic cycles, and they can be over-produced to make catalytic processes more economically efficient (Patel, 2004). This t ...
Characteristics of a Laboratory Strain of Coleomegilla maculata with
Characteristics of a Laboratory Strain of Coleomegilla maculata with

... (bab) [11]. Color patterns in wings of butterflies are complex, and may be the product of the co-option of developmental pathways, as exemplified by the eye development gene optix which is correlated with wing patterns of Heliconius butterflies [12]. The genes involved in color patterning in beetles ...
Genetics of mammalian meiosis: regulation, dynamics and impact
Genetics of mammalian meiosis: regulation, dynamics and impact

... (SMC1B)) and synaptonemal complex (SC)-specific proteins, such as SYCP3 and SYCP2. The chromatids experience genetically programmed double-strand DNA breaks (induced by SPO11), which provide the substrate for recombination (the two chromatids of the upper homologue are depicted by a turquoise line, ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
Promega Notes 49: Firefly Luciferase Engineered for Improved

... sequence modifications described above were designed wherever possible to yield more common codons (6). Also, where possible in our modifications strategy, we changed codons of ATA to ATC (Ile), GTA to GTG (Val), and TTA to CTG or CTC (Leu). These changes were chosen because they convert particularl ...
Lecture 3 - Montefiore Institute ULg
Lecture 3 - Montefiore Institute ULg

... Historical overview: associating genetic variation to disease outcomes ...
SNP Analysis of the PTC Gene Using PCR
SNP Analysis of the PTC Gene Using PCR

... (Figure 2). This fact was discovered in 1931 in a series of events that involved impressive scientific curiosity and questionable laboratory safety. A chemist named Arthur Fox was mixing a powdered chemical when he accidentally let a bit of the powder blow into the air. A nearby colleague exclaimed h ...
Gene Duplication - Semantic Scholar
Gene Duplication - Semantic Scholar

... respectively.  One  term  often  mentioned  in  the  human  and  medical  genetics  literature  is  “segmental  duplication,”  which  is  defined  as the  duplication  of  a  segment  of  DNA  that  has  1,000  nucleotides  or  more.  In  addition  to  unequal  crossing-­over,  segmental  duplicatio ...
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR
NOCARDIA sp. INDONESIAN VOLCANIC SOIL DESAK GEDE SRI ANDAYANI , ELIN YULINAH SUKANDAR

... resistance in cell microorganisms by changing the compound toxic to non-toxic. The bacteria that live in extreme environment have a mechanism of resistance toxic heavy metals such as selenium that only specific bacteria can survive on environment containing selenium. The mechanism of resistance is d ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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