The Plastidic Phosphoglucomutase from
... 1998). Deficiency in the plastidic PGM activity resulted in a “starchless” phenotype in Arabidopsis and N. sylvestris. The P. sativum PGM mutant, rug3, has a wrinkled seed phenotype with only 1% of the seed dry weight as starch, compared with 60% in the wild type (Harrison et al., 1998). The reducti ...
... 1998). Deficiency in the plastidic PGM activity resulted in a “starchless” phenotype in Arabidopsis and N. sylvestris. The P. sativum PGM mutant, rug3, has a wrinkled seed phenotype with only 1% of the seed dry weight as starch, compared with 60% in the wild type (Harrison et al., 1998). The reducti ...
Marine integrons containing novel integrase genes
... of the attI sites within an integron class is highly conserved. However, these sites, like the associated intI genes themselves are highly divergent in DNA sequence between classes. One of the most studied attI sites is attI1, the attachment site associated with class 1 integrons (Partridge et al., ...
... of the attI sites within an integron class is highly conserved. However, these sites, like the associated intI genes themselves are highly divergent in DNA sequence between classes. One of the most studied attI sites is attI1, the attachment site associated with class 1 integrons (Partridge et al., ...
Controlling morpholino experiments: don`t stop making antisense
... intended to inhibit translation has been extensively studied, and the rules for success prove to be relatively simple (see Box 1). There are so many examples of the use of translation-blocking MOs that it is difficult to select just a few to describe. Here, we mention two examples, one from each of ...
... intended to inhibit translation has been extensively studied, and the rules for success prove to be relatively simple (see Box 1). There are so many examples of the use of translation-blocking MOs that it is difficult to select just a few to describe. Here, we mention two examples, one from each of ...
Identification of New Genes Involved in Meiosis by a Genetic Screen
... Budding yeast Saccharomyces cerevisiae contains a group of proteins named ZMM that constitutes a link between recombination and Synaptonemal Complex (SC) assembly. Yeast mutants that lack ZMM proteins have defects in recombination, SC formation and nuclear division progression. Meiotic cell cycle pr ...
... Budding yeast Saccharomyces cerevisiae contains a group of proteins named ZMM that constitutes a link between recombination and Synaptonemal Complex (SC) assembly. Yeast mutants that lack ZMM proteins have defects in recombination, SC formation and nuclear division progression. Meiotic cell cycle pr ...
Computer-Aided DNA Base Calling from Forward and Reverse
... for each type of base). Fragments are then sorted by length by means of capillary electrophoresis and detected by 4 optical sensors working at disjoint wavelengths in order to distinguish the emissions of the 4 markers. The result of a sequencing experiment is an electropherogram that is a 4-compone ...
... for each type of base). Fragments are then sorted by length by means of capillary electrophoresis and detected by 4 optical sensors working at disjoint wavelengths in order to distinguish the emissions of the 4 markers. The result of a sequencing experiment is an electropherogram that is a 4-compone ...
Chapter 21
... An insertion sequence is a transposon that codes for the enzyme(s) needed for transposition flanked by short inverted terminal repeats. The target site at which a transposon is inserted is duplicated during the insertion process to form two repeats in direct orientation at the ends of the transposon ...
... An insertion sequence is a transposon that codes for the enzyme(s) needed for transposition flanked by short inverted terminal repeats. The target site at which a transposon is inserted is duplicated during the insertion process to form two repeats in direct orientation at the ends of the transposon ...
Nature Genetics: doi:10.1038/ng.3791
... Sequence variation between individuals or lines can induce variation in the ability to correctly map short sequence reads back to their location in a reference genome. Such sequence-specific biases in mapping can lead to artificial associations between genotype and any functional genomics measuremen ...
... Sequence variation between individuals or lines can induce variation in the ability to correctly map short sequence reads back to their location in a reference genome. Such sequence-specific biases in mapping can lead to artificial associations between genotype and any functional genomics measuremen ...
Alternative Splicing in Higher Plants
... For these alternative splicing isoforms that have a frameshift: - In Arabidopsis, 42% of these frameshifted events are found to introduce a premature stop codon >50bp upstream of the last exon-exon junction and are NMD candidates -In rice, this percentage is 36% This level of producing NMD candidate ...
... For these alternative splicing isoforms that have a frameshift: - In Arabidopsis, 42% of these frameshifted events are found to introduce a premature stop codon >50bp upstream of the last exon-exon junction and are NMD candidates -In rice, this percentage is 36% This level of producing NMD candidate ...
The universal reagent for genome tailoring
... genome. Furthermore, with the advent of mouse embryonic stem (ES) cells and homologous recombinationbased gene targeting, we are capable of removing any existing gene from the genome. By adding the Cre recombinase system, we can excise or invert loxP-flanked DNA segments or create intermolecular rec ...
... genome. Furthermore, with the advent of mouse embryonic stem (ES) cells and homologous recombinationbased gene targeting, we are capable of removing any existing gene from the genome. By adding the Cre recombinase system, we can excise or invert loxP-flanked DNA segments or create intermolecular rec ...
Chapter 4: Individual gene function
... Some loss-of-function alleles may still produce DNA, RNA, or protein, but still lack gene activity. This might happen if a gene lacks the ability to make the crucial functional domain of its product, it may not be able to function even if detectable protein product is present. For example, a gene en ...
... Some loss-of-function alleles may still produce DNA, RNA, or protein, but still lack gene activity. This might happen if a gene lacks the ability to make the crucial functional domain of its product, it may not be able to function even if detectable protein product is present. For example, a gene en ...
Genetic basis of mandibular prognathism
... The recent years have seen enormous development in the genetic methods employing modern technologies and allowing for a concurrent analysis of hundreds of thousands genetic polymorphisms located across the genome (the so-called GWAS – genome-wide association studies). The objective of GWAS is to ver ...
... The recent years have seen enormous development in the genetic methods employing modern technologies and allowing for a concurrent analysis of hundreds of thousands genetic polymorphisms located across the genome (the so-called GWAS – genome-wide association studies). The objective of GWAS is to ver ...
Gene Section RAD52 (RAD52 homolog (S. cerevisiae)) Atlas of Genetics and Cytogenetics
... Secondary structure of the hRAD52 protein. From Uniprot.org (Creative Commons License). ...
... Secondary structure of the hRAD52 protein. From Uniprot.org (Creative Commons License). ...
A survey of best practices for RNA-seq data analysis
... published, making it challenging for new users to appreciate all of the steps necessary to conduct an RNA-seq study properly. There is no optimal pipeline for the variety of different applications and analysis scenarios in which RNA-seq can be used. Scientists plan experiments and adopt different an ...
... published, making it challenging for new users to appreciate all of the steps necessary to conduct an RNA-seq study properly. There is no optimal pipeline for the variety of different applications and analysis scenarios in which RNA-seq can be used. Scientists plan experiments and adopt different an ...
The Genetics of Spinal Muscular Atrophy
... exon 7. (c) Point mutations can also be found in the SMN1 gene, but at a much lower frequency than the other two types of mutations. Shown here are the locations of point mutations that have been found in the SMN1 gene. They are labeled A through T. About 5% of 5q-SMA patients have a deletion or gen ...
... exon 7. (c) Point mutations can also be found in the SMN1 gene, but at a much lower frequency than the other two types of mutations. Shown here are the locations of point mutations that have been found in the SMN1 gene. They are labeled A through T. About 5% of 5q-SMA patients have a deletion or gen ...
PDF
... miss genuine similarities, then functional elements could be miss-assigned as non-functional. This uncertainty largely arises due to the unknown pattern of indels (gaps) between the pair of sequences [12]. A solution to this problem is to compute probabilities of alternative alignments according to ...
... miss genuine similarities, then functional elements could be miss-assigned as non-functional. This uncertainty largely arises due to the unknown pattern of indels (gaps) between the pair of sequences [12]. A solution to this problem is to compute probabilities of alternative alignments according to ...
Alterations to the remote control of Shh gene expression cause
... margin of the limb bud to the anterior margin during the early stages of development. The inducing tissue was defined as originating in the zone of polarizing activity (ZPA) of the limb bud and later the protein responsible for this activity was identified as SHH. We and others [29 –31] showed that ...
... margin of the limb bud to the anterior margin during the early stages of development. The inducing tissue was defined as originating in the zone of polarizing activity (ZPA) of the limb bud and later the protein responsible for this activity was identified as SHH. We and others [29 –31] showed that ...
Are Restriction Enzymes Recognition Sites Underrepresented in the
... The efficiency of restriction modification system II depends on the efficiency of both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentrat ...
... The efficiency of restriction modification system II depends on the efficiency of both enzymes, the effective recognition of the host restriction site sequence and the foreign restriction site sequence by the methyltransferase enzyme and restriction enzymes, respectively. The net cellular concentrat ...
Technique ChIC and ChEC: Genomic Mapping of Chromatin Proteins
... CEN plasmid. In a separate strain, the endogenous SIR3 gene of yeast was tagged with 13 myc epitopes (Sir3myc13). The ChIC procedure was then carried out in parallel with these myc-expressing cells and an untagged yeast strain. Samples were analyzed by SDSPAGE and Western blotting to follow the anti ...
... CEN plasmid. In a separate strain, the endogenous SIR3 gene of yeast was tagged with 13 myc epitopes (Sir3myc13). The ChIC procedure was then carried out in parallel with these myc-expressing cells and an untagged yeast strain. Samples were analyzed by SDSPAGE and Western blotting to follow the anti ...
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS)
... timing of record collection of statistical data for the measurement of outcome. This will result in accumulation of wealth of data that can be used for inter centre comparisons both for research and for audit in future. Gene Mapping Strategies:Gene mapping was carried out using Blood or tissue sampl ...
... timing of record collection of statistical data for the measurement of outcome. This will result in accumulation of wealth of data that can be used for inter centre comparisons both for research and for audit in future. Gene Mapping Strategies:Gene mapping was carried out using Blood or tissue sampl ...
Insertional inactivation studies of the csmA and csmC genes of the
... chlorosome proteins may have an important cellular function(s) other than light-energy harvesting. Since reaction center preparations of green sulfur bacteria typically contain substantial amounts of the FMO protein, which forms the baseplate of the chlorosome [2,27], one intriguing possibility is t ...
... chlorosome proteins may have an important cellular function(s) other than light-energy harvesting. Since reaction center preparations of green sulfur bacteria typically contain substantial amounts of the FMO protein, which forms the baseplate of the chlorosome [2,27], one intriguing possibility is t ...
The Mitochondrial Genome of Chara vulgaris
... mtDNA than Chaetosphaeridium mtDNA. The high similarity of codon usage observed for these mtDNAs also is seen at the level of stop codons. Five of the 11 Chara mitochondrial genes ending with TGA or TAG have counterparts with the same stop codon in Marchantia and/or angiosperm mtDNAs ( atp4, atp8, r ...
... mtDNA than Chaetosphaeridium mtDNA. The high similarity of codon usage observed for these mtDNAs also is seen at the level of stop codons. Five of the 11 Chara mitochondrial genes ending with TGA or TAG have counterparts with the same stop codon in Marchantia and/or angiosperm mtDNAs ( atp4, atp8, r ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
... ranged from 0 to 5 CFU on LB agar and R2A agar per 104 hyphal clusters. Each hyphal cluster contained between 102 and 103 Frankia genomes, so the measurable proportion of contaminating bacterial genomes was quite low. Phenolic compounds present in actinorhizal nodule homogenates normally turn bright ...
Lecture 3: Sequence Alignment
... on Needleman-Wunsch. As such, it has the desirable property that it is guaranteed to find the optimal local alignment with respect to the scoring system being used (which includes the substitution matrix and the gapscoring scheme). However, the Smith-Waterman algorithm is demanding of time and memor ...
... on Needleman-Wunsch. As such, it has the desirable property that it is guaranteed to find the optimal local alignment with respect to the scoring system being used (which includes the substitution matrix and the gapscoring scheme). However, the Smith-Waterman algorithm is demanding of time and memor ...