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Evolution of the Actin Gene Family in Testate Lobose Amoebae
Evolution of the Actin Gene Family in Testate Lobose Amoebae

... The evolution of actin gene families is characterized by independent expansions and contractions across the eukaryotic tree of life. Here, we assess diversity of actin gene sequences within three lineages of the genus Arcella, a free-living testate (shelled) amoeba in the Arcellinida. We established ...
Chapter 10: DIPLOIDY
Chapter 10: DIPLOIDY

... cell at fertilization. Haploid cells have half the number of chromosomes (n) as diploid - i.e. a haploid cell contains only one complete set of chromosomes. Haploid cells are found in various algae, various male bees, wasps and ants. Haploid cells should not be confused with monoploid cells as the m ...
Sequence Heterogeneities Among 16s
Sequence Heterogeneities Among 16s

... different but closely related genera (enterobacteria) and for different species or strains within a single genus (Escherichia or Salmonella), and finally how similar are the ribosomal operons within a single organism (Escherichia coli). These sequences have been analyzed by neighbor-joining, maximum ...
Homologous recombination in budding yeast expressing the human
Homologous recombination in budding yeast expressing the human

... Assaying DSB-stimulated ectopic gene conversion (EGC) Single colonies of strains of the appropriate genotype that contained the his3-Δ3 -HOcs allele at the HIS3 locus on chromosome XV, and the his3-ΔMscI allele at the LEU2 locus on chromosome III were used to inoculate a minimum of 10 one, or five ...
Slide 1
Slide 1

... Use either this parameter or blastResult_url parameter as input but not both together. blastResult_url: url of the blast result. .[BLAST_report]. oldRefFile_direct_data: old blast result file..[BLAST_report]. Use either this parameter or oldblastResult_url as input but not both together. oldRefFile_ ...
genomebiology.com
genomebiology.com

... revealed that TEs are less likely to be fixed within transcribed regions relative to orthologous regions in human and mouse [16]. In G. gallus, D. rerio and C. intestinalis, 33.2%, 47.3% and 39.4% of TEs reside within introns, respectively, whereas in the human genome, approximately 60% of TEs resid ...
pdf
pdf

... not only because of the uncertain physiological role it plays in these organisms, which require oxygen to oxidize ammonia and generate energy (17), but also because it has been demonstrated that ammonia oxidizers, such as Nitrosomonas europaea, produce the environmentally important trace gases nitri ...
Biology Recap
Biology Recap

... ■  Shared the power of microscopic observations ■  Defined term “cell” using the plant cork ...
Xylitol production using recombinant Saccharomyces
Xylitol production using recombinant Saccharomyces

... Meinander et al., 1996; Meinander and HahnHägerdal, 1997). And mitotically stable vectors, such as YIp, pYAC and pYCp, also have their own limitation for the mass production of heterologous proteins because the copy number is very low (1–2 copies cell − 1) (Romanos et al., 1992). To circumvent thes ...
Author`s personal copy - Real Jardín Botánico
Author`s personal copy - Real Jardín Botánico

... entire plant kingdom asserting that plants generally possess separate arrays. This study reveals that plant nrDNA organization into separate arrays is not a distinctive feature, but rather assignable almost solely to seed plants. We show that early diverging land plants and presumably streptophyte a ...
Nucleotide sequence analysis - Bioinformatics Unit
Nucleotide sequence analysis - Bioinformatics Unit

... This program is much more sensitive than BLAST programs, which is reflected by the length  of  time  required  to  produce  results.  FASTA  produces  optimal  local  alignment  scores  for  the  comparison of the query sequence to every sequence in the database.  ...
What`s new - JSI medical systems
What`s new - JSI medical systems

... The entry in Suffix can have up to six characters. The suffix is added to the Name of the ROI, e.g: Suffix ng (for NimbleGene), ROI name is BRCA1E01-ng. Therefore all ROIs added on tab Enrichment have a suffix in the ROI name listed in column Name. There is the setting auto cut available: If you use ...
1 Defining the epigenetic mechanism of asymmetric cell division of
1 Defining the epigenetic mechanism of asymmetric cell division of

... We determined the efficiency and the switching pattern of S. japonicus var. versatilis strain Sj4 cells by quantifying zygote formation among four granddaughter progeny of a single cell. Luckily, unlike S. pombe, S. japonicus cell pairs of opposite mating type readily mate without requiring further ...
Developing expressed sequence tag libraries and
Developing expressed sequence tag libraries and

... sequenced, quality verified, assembled and scanned for SSRs. Primers flanking the SSRs were designed and a subset tested for amplification, polymorphism and transferability across species. ESTs containing SSRs were functionally annotated using the GenBank non-redundant (nr) database and further clas ...
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics

Establishment of new mutations under divergence and genome
Establishment of new mutations under divergence and genome

... speciation, where gene flow constantly introduces the wrong combination of genes into a local population [17,22–24]. These considerations apply when speciation is initiated in the face of gene flow, as well as when some divergence initially occurs in allopatry and then a period of secondary contact ...
ANSWER KEY
ANSWER KEY

... able to swim. Justify your choice in two sentences or less. NONE. To obtain KanR, the phage genome has to integrate into the att site of the E. coli genome by homologous recombination. As the att site is contained in a gene required for swimming—and the recombination event will eliminate the functio ...
Cloning and expression of the phosphotriesterase
Cloning and expression of the phosphotriesterase

... enzymic activity in library clones. In a previous study, an OP-degrading gene was successfully isolated from an Agrobacterium strain by mobilizing a genomic library into a related strain lacking activity (Horne et al., 2002a). A similar approach was taken here, using Pseudomonas aeruginosa BENNY, a ...
Isolation, Characterization, and Annotation: The Search for Novel
Isolation, Characterization, and Annotation: The Search for Novel

... to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysogenic cycle, the DNA is incorporated into the host genome as a prophage and remains a part of the host’s genome as long as conditions remain stable for the prophage. ...
rpoB gene sequence-based characterization of emerging non
rpoB gene sequence-based characterization of emerging non

lecture 1 File
lecture 1 File

... recombinations more likely than mutations to provide beneficial change since it tends not to destroy gene function Eukaryotes: recombination during meiosis for sexual reproduction -creates diversity in offspring but parent remains unchanged -vertical gene transfer = genes passed from organism to off ...
Discovery and characterization of chromatin states for Please share
Discovery and characterization of chromatin states for Please share

... 2.7% genome-wide (P<10−200, for all states). These states accounted for 59% of all RefSeq TSS while covering only 1.3% of genome. These states all had in common a high frequency of H3K4me3, but differed in terms of other associated marks, primarily H3K79me2/3, H4K20me1, H3K4me1/2, and H3K9me1, and t ...
Massive Changes in Genome Architecture Accompany
Massive Changes in Genome Architecture Accompany

... Present address: Fox Chase Cancer Center, Philadelphia, PA 19111. ...
CS790 – Introduction to Bioinformatics
CS790 – Introduction to Bioinformatics

... GCG-TIntro to Bioinformatics – Sequence Alignment ...
High-Resolution Single-Copy Gene Fluorescence in Situ
High-Resolution Single-Copy Gene Fluorescence in Situ

... been distinctly illustrated by the molecular and cytological characterization of a heterochromatic knob on the short arm of Arabidopsis thaliana chromosome 4 (Fransz et al., 2000) and the confirmation of anchored BAC contigs and gene orders in rice (Oryza sativa) (Zhao et al., 2002). In maize, howev ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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