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When Christian Faith and Genetics Meet
When Christian Faith and Genetics Meet

... September, 1999, to advise the government of the day and to engage Canadians regarding the development and regulation of biotechnologies for the benefit and protection of the public. CBAC had its sceptics who felt that CBAC accepted biotechnology too uncritically, but at least it sought to be consul ...
BROWSING GENES AND GENOMES WITH ENSEMBL
BROWSING GENES AND GENOMES WITH ENSEMBL

... (c) Have a look at the external references for ENST00000218099. What is the function of F9? (d) Is it possible to monitor expression of ENST00000218099 with the ILLUMINA HumanWG_6_V2 microarray? If so, can it also be used to monitor expression of the other two transcripts? (e) In which part (i.e. th ...
cbb752-mg-spr10-bioinfo-intro
cbb752-mg-spr10-bioinfo-intro

... Mark Gerstein, Yale University gersteinlab.org/courses/452 (last edit in spring '10) ...
Genetic Epidemiology Association Studies and Power Considerations
Genetic Epidemiology Association Studies and Power Considerations

... It is generally accepted that, for most human populations and most regions of the genome, substantial linkage disequilibrium is only likely to occur between loci with a recombination fraction of less than 1%. Thus, LD mapping is most useful for fine mapping over small distances or for recent mutatio ...
Mutualism and asexual reproduction influence recognition genes in
Mutualism and asexual reproduction influence recognition genes in

... within the rab1, ITS, and Tef-1a regions examined. The recombination parameter R was calculated (Hudson et al. 1987), while the minimum number of recombination (RM) events during the history of the species (Hudson & Kaplan 1985) was estimated using neutral coalescence simulations, based on the numbe ...
Characterisation of marsupial PHLDA2 reveals eutherian specific acquisition of imprinting Open Access
Characterisation of marsupial PHLDA2 reveals eutherian specific acquisition of imprinting Open Access

... A 272 bp fragment was amplified by RT-PCR using a primer pair designed to a highly conserved sequence in the open reading frame (ORF) of the PHLDA2 gene among multiple species. Given the PCR product sequence was highly similar to PHLDA2 of other species, we next carried out 3’ RACE to obtain 3’ UTR ...
Origin and evolution of the slime molds (Mycetozoa)
Origin and evolution of the slime molds (Mycetozoa)

... Mycetozoan tef Gene Sequences and Intron Positions. The 59 two-thirds of the Physarum tef gene was amplified from genomic DNA, while the 39 half of the gene was amplified from cDNA. The latter was necessary because all primer combinations for the 39 half of the gene preferentially amplified the retr ...
AS Biology Contents Guide
AS Biology Contents Guide

... Guide to negative feedback Overview of positive feedback Comparing nervous and endocrine control Deciding how different conditions are controlled Behavioural controls, including kineses and taxes Using graphs to visualize homeostatic mechanisms ...
Evolutionary origin and consequences of uniparental mitochondrial
Evolutionary origin and consequences of uniparental mitochondrial

... within the population of (normal) hermaphroditic plants and plants that produce only seeds but no viable pollen. Much genetic and population genetic research on this trait has been carried out in natural populations of Plantago and Thymus, as well as in several agricultural crops. At first sight, th ...
CHAPTER 14 DNA applications in society
CHAPTER 14 DNA applications in society

... the growth of all body tissues, including bones. hGH was first isolated from human pituitary glands in 1956. By the 1960s, hGH was in clinical use in the treatment of children and adolescents with a particular growth deficiency. This treatment often involved the subcutaneous injection of growth horm ...
DNA Sequence Changes of Mutations Altering
DNA Sequence Changes of Mutations Altering

... operon, upstream of the his0 mutation of interest, and a Tn5 (kanamycin resistance) insertion in the h&G gene. These strains (TT3602, ‘I”I’3603, TT3604) were used as donors in transductional crosses with a his01242, hisG double mutant as recipient (TR3063). By selecting the tetracycline-resistant tr ...
Distinct functions of two olfactory marker protein genes derived from
Distinct functions of two olfactory marker protein genes derived from

... sequences of these OMP homologs (Fig. 2). Amino acids sequences are conserved among teleost OMP1 and OMP2 and tetrapod OMP. In particular, the Eph2B-receptor-like loop domain, a potentially key region for OMP function as a molecular switch [35], is highly conserved. Thus, the fundamental structure a ...
DFL1, an auxin-responsive GH3 gene homologue, negatively
DFL1, an auxin-responsive GH3 gene homologue, negatively

... plants were grown on a medium containing a low concentration of IAA (10±10 and 10±9 M), no signi®cant differences in primary root elongation were observed between d¯1-D and wild type (Figure 5). At a relatively high concentration (10±7 M), while the growth of wildtype roots was strongly inhibited, t ...
The biased nucleotide composition of the HIV genome: a constant
The biased nucleotide composition of the HIV genome: a constant

... O (35%, Table 1). Group N and P viruses appear to contain slightly higher (group N) or lower (group P) levels of A-nucleotides, but only one (group P) or no (group N) full-length genomes with long terminal repeats (LTRs) are available for these groups (Table 1). As the LTR is relatively A-poor [5], ...
Biotechnology Timeline
Biotechnology Timeline

... Advances in 3D printing technology lead to “skin printing.” European scientists begin clinical trials for an anti-HIV biotech medicine produced using genetically modified tobacco. This increases the potential for cost-effective HIV/AIDS therapy in the developing world. ...
Harrisslides
Harrisslides

Week 2. DNA isolation and PCR
Week 2. DNA isolation and PCR

Correlation of amino acid preference and
Correlation of amino acid preference and

... amino acid preference is used to describe the preference for certain ordered amino acid triples. Three types of analysis are conducted. At the genome level, crossvalidation is used to demonstrate the predictive capability of amino acid preference of viral genome type. Next at the sequence level, two ...
Seed-Specific Gene Activation Mediated by the Cre//ox Site
Seed-Specific Gene Activation Mediated by the Cre//ox Site

... analyzing gene expression after Cre-mediated activation, the timing and patterns of expression of the promoters of the genes encoding French bean (Phaseolus vulgaris) &phaseolin and the a' subunit of soybean (G/ycine max) &conglycinin, as well as the cauliflower mosaic virus 35s promoter, were studi ...
Mating-Type Genes From the Homothallic Fungus Sordaria
Mating-Type Genes From the Homothallic Fungus Sordaria

... that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region is shown in Figure 1. DNA sequence analysis: To ...
Developing molecular methods to identify and quantify ballast water
Developing molecular methods to identify and quantify ballast water

... characterization of the taxa present in ballast water samples is often restricted to the taxonomic level of order, class or even phylum. The goal of this project was to adapt standard molecular methods into a novel approach for quantifying the abundance and diversity of organisms in the ballast wate ...
- Philsci-Archive
- Philsci-Archive

... described as more or less contingent, specific, or framed at an appropriate or inappropriate level, to explore some of the interrelationships among these notions, and locate them notions within a larger framework for discussing causation and explanation. I will also try to illustrate how a concern w ...
Genome-Wide Dissection of Hybrid Sterility in
Genome-Wide Dissection of Hybrid Sterility in

... to a threshold character whose developmental reaction norm is disrupted by the genomic stress elicited in hybridization. Evidence accumulated during several decades of research (Cabot et al. 1994; Moyle and Nakazato 2009; Chang et al. 2010; Nosil and Schluter 2011) has reached a point of minimum con ...
Nomenclature for factors of the Dog Major
Nomenclature for factors of the Dog Major

... sequenced, etc. We will also record the breed of dog in which the sequence was found. This data will not be made public at this time, however, as such information may cause assumptions to be made about the restriction of particular alleles to certain breeds. (This is in keeping with the policy of th ...
The Plastidic Phosphoglucomutase from
The Plastidic Phosphoglucomutase from

... 1998). Deficiency in the plastidic PGM activity resulted in a “starchless” phenotype in Arabidopsis and N. sylvestris. The P. sativum PGM mutant, rug3, has a wrinkled seed phenotype with only 1% of the seed dry weight as starch, compared with 60% in the wild type (Harrison et al., 1998). The reducti ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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