Staphylococcus aureus CC395 harbours a novel
... in JS395 were nearly identical to sequences from an S. aureus phage, GRCS, isolated from raw sewage in India,20 and a plasmid, SAP020A, isolated from a CoNS species (DDBJ/EMBL/GenBank accession number GQ900386), respectively. Together, these findings support horizontal transfer of the CRISPR locus b ...
... in JS395 were nearly identical to sequences from an S. aureus phage, GRCS, isolated from raw sewage in India,20 and a plasmid, SAP020A, isolated from a CoNS species (DDBJ/EMBL/GenBank accession number GQ900386), respectively. Together, these findings support horizontal transfer of the CRISPR locus b ...
Full-Text PDF
... 22, 25, 28, 30, 35, 37, 40 and 45 °C and pH 4.5, 5, 5.5, 6.0, 6.5, 6.8, 7.2, 7.5 and 8.0. Nitrogen sources were tested by using liquid LMM medium in which KNO3 (0.1 g·L−1) was replaced by 0.1 g·L−1 of NH4Cl, methylamine or glycine. The growth of strains was also tested in triplicate with nitrogen-fr ...
... 22, 25, 28, 30, 35, 37, 40 and 45 °C and pH 4.5, 5, 5.5, 6.0, 6.5, 6.8, 7.2, 7.5 and 8.0. Nitrogen sources were tested by using liquid LMM medium in which KNO3 (0.1 g·L−1) was replaced by 0.1 g·L−1 of NH4Cl, methylamine or glycine. The growth of strains was also tested in triplicate with nitrogen-fr ...
A Cre Transcription Fidelity Reporter Identifies GreA as a
... resistant and sucrose sensitive (Figure S1C). We then used PCR to amplify DNA from construct of Figure S1B, so as to include loxP and the distal galK segment through to the end of galM. The PCR primers used for amplification carried homologies on each 5’ end designed to insert the loxP galK galM DNA ...
... resistant and sucrose sensitive (Figure S1C). We then used PCR to amplify DNA from construct of Figure S1B, so as to include loxP and the distal galK segment through to the end of galM. The PCR primers used for amplification carried homologies on each 5’ end designed to insert the loxP galK galM DNA ...
Distinguishing Different DNA Heterozygotes by
... them. In our study, high-resolution melting of small amplicons distinguished all heterozygotes studied. This included 21 pairwise comparisons, suggesting that most randomly selected heterozygotes within small amplicons can be distinguished. The power of melting analysis to distinguish multiple seque ...
... them. In our study, high-resolution melting of small amplicons distinguished all heterozygotes studied. This included 21 pairwise comparisons, suggesting that most randomly selected heterozygotes within small amplicons can be distinguished. The power of melting analysis to distinguish multiple seque ...
Interphase chromosome remodelling - Journal of Cell Science
... chromosomal region. This was confirmed by double-labelling with both rye genomic probe and a probe to the rDNA (data not shown). Induced changes in DNA methylation or histone acetylation cause remodelling of interphase chromosome territory organisation To determine whether changes in the DNA methyla ...
... chromosomal region. This was confirmed by double-labelling with both rye genomic probe and a probe to the rDNA (data not shown). Induced changes in DNA methylation or histone acetylation cause remodelling of interphase chromosome territory organisation To determine whether changes in the DNA methyla ...
M.Tevfik Dorak, BA (Hons), MD, Ph.D.
... Adenosine deaminase deficiency - causes a complete failure of the immune system. It is one of the few diseases which have been successfully treated by gene therapy. Bone marrow cells from patients have been removed, a working gene has been placed into them and the cells have been replaced restoring ...
... Adenosine deaminase deficiency - causes a complete failure of the immune system. It is one of the few diseases which have been successfully treated by gene therapy. Bone marrow cells from patients have been removed, a working gene has been placed into them and the cells have been replaced restoring ...
Running title Variation in Barley Brassinosteroid
... brh3.h, brh3.y, and ert-t.55. In the barley genome zipper, the common genetic donor interval of those lines contained 21 gene models but did not include the Hv-BRD ortholog of Brachypodium, rice, and sorghum. In EnsemblPlant (http://plants.ensembl.org), the same interval contained 68 gene models inc ...
... brh3.h, brh3.y, and ert-t.55. In the barley genome zipper, the common genetic donor interval of those lines contained 21 gene models but did not include the Hv-BRD ortholog of Brachypodium, rice, and sorghum. In EnsemblPlant (http://plants.ensembl.org), the same interval contained 68 gene models inc ...
Nucleotide sequence and taxonomic value of the
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
... 'distance' between pairs of species from DNA sequences which are then utilized by the distance matrix programs FITCH and KITSCH. Sequences were first aligned with the multiple alignment program CLUSTAL (Higgins & Sharp, 1988) so that they contained the same number of characters including padding cha ...
Bioinformatics Sequencing
... – Phylogenetic trees can be constructed based on comparison of the sequences of a molecule (example: 16S rRNA) taken from different species – Residues conserved during evolution play an important role ...
... – Phylogenetic trees can be constructed based on comparison of the sequences of a molecule (example: 16S rRNA) taken from different species – Residues conserved during evolution play an important role ...
The Evolutionary Accessibility of New Enzyme Functions: A Case
... family member provided a starting point for conversions to new but related functions. In fact, despite their similarities, these families can include remarkable functional diversity. Here we focus not on minor functional variations within families, but rather on innovations—transitions to genuinely ...
... family member provided a starting point for conversions to new but related functions. In fact, despite their similarities, these families can include remarkable functional diversity. Here we focus not on minor functional variations within families, but rather on innovations—transitions to genuinely ...
exploring genetics - Cold Spring Harbor Laboratory
... Standard Course of Study for the middle school grades. The career information, reflection activities, and the inquiry based nature of the activities provide a well rounded introduction to genetic science and its relation to mathematics at the middle grades level. The kit was developed as an outreach ...
... Standard Course of Study for the middle school grades. The career information, reflection activities, and the inquiry based nature of the activities provide a well rounded introduction to genetic science and its relation to mathematics at the middle grades level. The kit was developed as an outreach ...
IlllllllllllllIlllllllIllllllllllllllIlllllllllllllllllllllllllllllllllllll
... The tac promoted toxin gene found in pMYC436 40 (pMYC47l, pMYC485, and pMYC1l6l) is constitutive and occurs independently of the concentrations of lac (NRRL deposit no. B-l8292) was cloned as a 4.5 Kbp tose or glucose present in the cell. For example, in the BamHI to PstI fragment in the vector pTJ ...
... The tac promoted toxin gene found in pMYC436 40 (pMYC47l, pMYC485, and pMYC1l6l) is constitutive and occurs independently of the concentrations of lac (NRRL deposit no. B-l8292) was cloned as a 4.5 Kbp tose or glucose present in the cell. For example, in the BamHI to PstI fragment in the vector pTJ ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... P22pro-I and P22pro-3 are specialized transducing derivatives of phage P22 that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we fo ...
... P22pro-I and P22pro-3 are specialized transducing derivatives of phage P22 that carry the proA and proB genes of Salmonella typhimurium. These genes lie immediately adjacent to the prophage attachment site on the bacterial chromosome. By examining DNA heteroduplexes in the electron microscope, we fo ...
The tightly regulated promoter of the xanA gene of
... have shown that this enzyme is responsible for the utilization of xanthine as nitrogen source in Schizosaccharomyces pombe, which lacks xanthine dehydrogenase, and homologues are present in a number of fungi, including many yeasts able to utilize oxidized purines as sole nitrogen sources. Many fungi ...
... have shown that this enzyme is responsible for the utilization of xanthine as nitrogen source in Schizosaccharomyces pombe, which lacks xanthine dehydrogenase, and homologues are present in a number of fungi, including many yeasts able to utilize oxidized purines as sole nitrogen sources. Many fungi ...
Tutorial: Mendelian Genetics - Integrated DNA Technologies
... References and Resources ................................................................................................ 10 Supplemental Material ..................................................................................................... 11 ...
... References and Resources ................................................................................................ 10 Supplemental Material ..................................................................................................... 11 ...
Alu
... Place K points into the space represented by the objects that are being clustered. These points ...
... Place K points into the space represented by the objects that are being clustered. These points ...
Journal of Autoimmunity - The Lu
... rearranged V, D (-chain only), J, and C gene segments. The recognition of antigens by -TCRs is mediated, in part, by complementarity determining regions (CDR3) spanning the V(D)J junctions of rearranged TCR genes [42]. CDR3 sequence diversity is enhanced further by the addition and deletion of ra ...
... rearranged V, D (-chain only), J, and C gene segments. The recognition of antigens by -TCRs is mediated, in part, by complementarity determining regions (CDR3) spanning the V(D)J junctions of rearranged TCR genes [42]. CDR3 sequence diversity is enhanced further by the addition and deletion of ra ...
The photosynthetic apparatus of Prochlorococcus
... supported clade, which can be further divided into two clusters (Urbach et al., 1998; Rocap et al., 1999; Fig. 2). In contrast, the high B/A isolates, while distinctly different from the low B/A isolates, do not form a monophyletic clade of their own but instead are divided among four clusters (Roca ...
... supported clade, which can be further divided into two clusters (Urbach et al., 1998; Rocap et al., 1999; Fig. 2). In contrast, the high B/A isolates, while distinctly different from the low B/A isolates, do not form a monophyletic clade of their own but instead are divided among four clusters (Roca ...
Lecture-Mic 623-Plasmids-Listeria - Home
... into four groups, the smallest being 5% of cultures resistant to both agents, and the largest (53%) being sensitive to cadmium and resistant to arsenic. The resistance patterns to these agents and the presence of plasmid DNA varied markedly between the serotypes of the cultures. The detection of pla ...
... into four groups, the smallest being 5% of cultures resistant to both agents, and the largest (53%) being sensitive to cadmium and resistant to arsenic. The resistance patterns to these agents and the presence of plasmid DNA varied markedly between the serotypes of the cultures. The detection of pla ...
The Johns Hopkins University - American University of Beirut
... • You need to understand the data they have, and how it is organized • There are often many ways to get to an answer. • Route to get there is not always obvious, but you need to think of alternatives and traps. • Use some query language – each system has its own. • Retrieve data in a specified forma ...
... • You need to understand the data they have, and how it is organized • There are often many ways to get to an answer. • Route to get there is not always obvious, but you need to think of alternatives and traps. • Use some query language – each system has its own. • Retrieve data in a specified forma ...