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Genomic Analysis of Hox Clusters in the Sea Lamprey
Genomic Analysis of Hox Clusters in the Sea Lamprey

... amphioxus genes, one or more lamprey homeodomains clustered with the complete complement of mouse cognates for groups 1, 2, 3, 8, and 11 with bootstrap confidence levels of 97% or greater. Groups 4, 9, and 10 are recovered at lower bootstrap proportions. Because of the high degree of amino acid simi ...
GENOTYPE-PHENOTYPE CORRELATION USING
GENOTYPE-PHENOTYPE CORRELATION USING

... Through the course of this dissertation, I am indebted to many people who I interacted with. My deepest thanks go to my advisers Dr. Ralf Bundschuh and Dr. Daniel Janies. I would like to thank them not only for their guidance but also the way they have supported me. I was very lucky to have two advi ...
Linkage Disequilibrium and Inference of Ancestral
Linkage Disequilibrium and Inference of Ancestral

... integrates the effects of mutation, drift, and recombination in giving rise to a particular statistical association across sites. For example, in a population that is in steady state with respect to accumulation of neutral mutations, random drift, and recombination, the expected LD is E(r 2 ) p 1/(1 ...
Dissecting plant meiosis using Arabidopsis thaliana mutants
Dissecting plant meiosis using Arabidopsis thaliana mutants

... cells (a mitosis-like division). Agashe et al. (2002) report that in the dyad mutant GUS (b-glucuronidase) expression is driven from a DMC1 promoter during female meiosis suggesting that this division may be meiotic rather than mitotic in nature. Subsequently, these cells can undergo either a second ...
Construction of a genetic linkage map of Thlaspi
Construction of a genetic linkage map of Thlaspi

... library of T. caerulescens accession LC (D. Rigola & M. G. M. Aarts, unpublished results), for which homologous genes were found in Arabidopsis and which were evenly distributed over the Arabidopsis genome. Fragments of T. caerulescens genes, represented by the selected ESTs, were amplified from gen ...
WW Genetic Counselor English - Wonderwise
WW Genetic Counselor English - Wonderwise

... extensions. Activities can be used in any order. Also included are objectives and learning outcomes, assessment questions, ideas for a presentation or exhibit and topics for further investigation. Wonderwise learning outcomes are based on national science education standards identified by McREL (Mid ...
π, γ
π, γ

... union of a(Π ) and a(Γ ), where adjacencies of Γ are colored red (Fig Observe t hat B (Π , Γ ) is also a disjoint union of pat hs and cycles, which al between red and blue edges. T he length of a component of B(Π , Γ ) is it s n The Double-Cut-and-Join Operation of edges; we consider an isolat ed v ...
Complete comparative genomic analysis of two field isolates of
Complete comparative genomic analysis of two field isolates of

... six insertions totalling 30 bp and 20 deletions totalling 1326 bp occur in predicted ORFs; 65 point mutations, three insertions (13 bp) and five deletions (102 bp) occur in intergenic regions; and 58 point mutations, two insertions (79 bp) and six deletions (98 bp) occur in homologous repeated (hr) ...
2q13 microduplications
2q13 microduplications

... With any duplication, the amount of duplicated DNA can vary. If the amount is small it may not be possible to see it under the microscope and many people who have a microduplication may have previously been told their standard chromosome analysis was ‘normal’. A laboratory technique called FISH (flu ...
Arc Diagrams: Visualizing Structure in Strings
Arc Diagrams: Visualizing Structure in Strings

... is written in Java, runs efficiently on a low-end (266 Mhz Pentium II) machine, and can create diagrams of sequences of several thousand symbols within seconds. To enumerate repeated patterns, a suffix tree is constructed and traversed twice. In the first pass, repetition regions are identified and ...
In Silico method for identification of MHC class I
In Silico method for identification of MHC class I

... pig, dog) are highly consistent with the published data. Overall, when applied to species that have not  been studied before, we find a highly variable number of CD1 genes and only one or two MR1 genes. The  variability in size of the CD1 loci is in line with the sizes of the CD1 family indicated by ...
Creating conditional dual fluorescence labelled transgenic animals
Creating conditional dual fluorescence labelled transgenic animals

... critical to the field. We developed a fast and straightforward method to determine ncRNAtransgene copy number, orientation, and insertion site in the genome. Furthermore, upon tissuespecific expression of ncRNA, a Cre-loxP mediated dual-fluorescence expression system facilitates fluorescence signal ...
Repetitive complete hydatidiform mole can be biparental in origin
Repetitive complete hydatidiform mole can be biparental in origin

... second set of chromosomes being inherited from the mother as in a normal pregnancy. The rarity of these cases makes it difficult to estimate their true frequency. However, a recent study of two families in which several sisters had one or more CHM, found that all CHM examined were biparental in orig ...
Analysis of expressed sequence tags from the Huperzia serrata leaf
Analysis of expressed sequence tags from the Huperzia serrata leaf

... nucleotide sequences from H . serrata available in the NCBI database. The limited information on the genetic contents of this plant triggered our efforts to construct a cDNA library from the H . serrata leaf. The objective of this study was to identify the functional genes in H . serrata, especially ...
7nQ Jj I f NO "7^07 - UNT Digital Library
7nQ Jj I f NO "7^07 - UNT Digital Library

... order to excel in this course. The first three lectures are devoted to DNA, mitosis, and meiosis. These seem to be the most difficult concepts for students to grasp. I intend on making certain that all the students are familiar with these topics, otherwise they will be confused during the remaining ...
Myb genes enhance tobacco trichome production
Myb genes enhance tobacco trichome production

... the same plant of a given species, with type and spacing determined by the plant organ or organ surface (ie, adaxial vs. abaxial, leaf vs. stem) from which the trichome extends, and the developmental phase of the plant at the time of organ initiation. The trichome is a useful cell type for studying ...
Heavy Chain Diversity Region Segments of the Channel Catfish
Heavy Chain Diversity Region Segments of the Channel Catfish

M.Sc., Biotechnology - Alagappa University
M.Sc., Biotechnology - Alagappa University

... of diseases and in designing appropriate therapies. Currently the smart use of various biochemical laboratory tests have become an integral part of disease diagnosis and monitoring the treatment. A high understanding of biochemistry and of other related basic disciplines such as cell biology, molecu ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
DNA MUTATION, REPAIR, AND TRANSPOSITION

... the mutation. Single base-substitutions, on the other hand, only affect a single codon. Therefore, frameshift mutations will have a more deleterious effect than basesubstitution mutations because they affect a larger proportion of amino acids in the polypeptide. 26. A genetic system that correctly r ...
Contrasting Properties of Gene-Specific Regulatory, Coding, and
Contrasting Properties of Gene-Specific Regulatory, Coding, and

... Genetic dissection of phenotypic differences within and between species has shown that mutations affecting either the expression or function of a gene product can contribute to phenotypic evolution; mutations that alter gene copy number have also been shown to be an important source of phenotypic va ...


Finishing the Human Genome
Finishing the Human Genome

... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
Bioinformatics: Bringing it all together
Bioinformatics: Bringing it all together

... maintained by the Supercomputer Center at the University of California, San Diego, is particularly popular, offering more than 80 bioinformatics tools to more than 10,000 registered users. "It's a one-stop-shop for doing a lot of things," says lead developer Shankar Subramaniam. "You can be sitting ...
Supplementary Data - Word file
Supplementary Data - Word file

... As described in the main text, an examination of the pattern of pairwise syteny between the three Aspergilli reveals large regions lacking detectable long range synteny. Repeat sequences are enriched in these non-syntenic regions, with 40% of A. nidulans repeats sequences in breaks with both A. fumi ...
Novel Blocked-Cleavable Primers for Quantitative Detection of
Novel Blocked-Cleavable Primers for Quantitative Detection of

... Single nucleotide polymorphisms (SNPs) are common and often correlate with important biological traits. The ability to accurately discriminate between different alleles is critical for modern diagnostics. A mismatch at or near the RNA base has a large effect on the ability of RNase H2 to cleave a bl ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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