Identification of editing positions in the ndhB transcript from maize
... in the same orientation and in close proximity by the rpsl gene and the 3'exon of the rps\2 gene. This suggests that the three units are cotranscribed in the form of a larger transcript as is common for other gene clusters occurring in the plastomes of higher plants (27). In Figure 2 the nucleotide ...
... in the same orientation and in close proximity by the rpsl gene and the 3'exon of the rps\2 gene. This suggests that the three units are cotranscribed in the form of a larger transcript as is common for other gene clusters occurring in the plastomes of higher plants (27). In Figure 2 the nucleotide ...
Full text - PAHdb - McGill University
... genomic sequences are both visible online in PAHdb. cDNA sequences have long been available [Kwok et al., 1985; Konecki et al., 1992] but the genomic sequence is recent (Konecki, D.S., unpublished; deposited in PAHdb Nov. 2001). The genomic sequence of the PAH gene and its flanking regions spans 171 ...
... genomic sequences are both visible online in PAHdb. cDNA sequences have long been available [Kwok et al., 1985; Konecki et al., 1992] but the genomic sequence is recent (Konecki, D.S., unpublished; deposited in PAHdb Nov. 2001). The genomic sequence of the PAH gene and its flanking regions spans 171 ...
Sex-specific Trans-regulatory Variation on the Drosophila melanogaster X Chromosome
... even reversed. In species where neither the X chromosome nor the autosomes recombine in males (e.g. Drosophila), the rate of recombination could be lower on the autosomes. Lower autosomal rates of recombination would, because of Hill-Robertson interference, result in a larger reduction in the effect ...
... even reversed. In species where neither the X chromosome nor the autosomes recombine in males (e.g. Drosophila), the rate of recombination could be lower on the autosomes. Lower autosomal rates of recombination would, because of Hill-Robertson interference, result in a larger reduction in the effect ...
Bioinformatics
... information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipline with many applications. ...
... information associated with these molecules, on a large-scale. • Bioinformatics is a practical discipline with many applications. ...
29th International Conference on Animal Genetics ISAG2004/TOKYO
... Construction of a high resolution comparative gene map between human chromosome 14 and swine chromosomes using RH mapping. Hiroshi Yasue (National Institute of Agrobiological Sciences) ...
... Construction of a high resolution comparative gene map between human chromosome 14 and swine chromosomes using RH mapping. Hiroshi Yasue (National Institute of Agrobiological Sciences) ...
Figures and figure supplements
... Figure 2—figure supplement 4. ChIP-seq profiles of H3K4me3 and total histone H3 across high-variance and low-variance genes. (A) Developmental expression profile, from early cleavage to adult, of the highly expressed high-variance genes (n = 1066). Expression levels were measured by CEL-seq and resc ...
... Figure 2—figure supplement 4. ChIP-seq profiles of H3K4me3 and total histone H3 across high-variance and low-variance genes. (A) Developmental expression profile, from early cleavage to adult, of the highly expressed high-variance genes (n = 1066). Expression levels were measured by CEL-seq and resc ...
KAIE LOKK Comparative genome-wide DNA methylation
... the proportion of methylated CpG sites can vary greatly over a genome. Repeated sequences tend to be the most heavily methylated, while CpG-rich regulatory regions are almost exclusively unmethylated in all human tissues. As DNA methylation is vital for the normal functioning of organism, changes in ...
... the proportion of methylated CpG sites can vary greatly over a genome. Repeated sequences tend to be the most heavily methylated, while CpG-rich regulatory regions are almost exclusively unmethylated in all human tissues. As DNA methylation is vital for the normal functioning of organism, changes in ...
Two distinct teleost hepatocyte nuclear factor 1 genes, hnf1a/tcf1
... 2.3. HNF1 transcripts detection by two-step RT-PCR Total RNAs from various tissues of adult zebrafish and developmental stages of zebrafish embryo were prepared by using TRIZOL reagent (Invitrogen). Using 1 Ag of total RNA, the first-strand cDNAs (20 Al) were synthesized with oligo(dT) by SUPERSCRIP ...
... 2.3. HNF1 transcripts detection by two-step RT-PCR Total RNAs from various tissues of adult zebrafish and developmental stages of zebrafish embryo were prepared by using TRIZOL reagent (Invitrogen). Using 1 Ag of total RNA, the first-strand cDNAs (20 Al) were synthesized with oligo(dT) by SUPERSCRIP ...
Transcript Isoform Differences Across Human Tissues Are
... Important biological processes are regulated by the expression of alternative isoforms 8,9 , and their mis-regulation has been observed in many diseases, including cancer 10,11,12,13 . For dozens of genes, it has been experimentally demonstrated that alternative isoforms result in proteins with diff ...
... Important biological processes are regulated by the expression of alternative isoforms 8,9 , and their mis-regulation has been observed in many diseases, including cancer 10,11,12,13 . For dozens of genes, it has been experimentally demonstrated that alternative isoforms result in proteins with diff ...
Phylogenetic Relationships between the Western Aster Yellows
... The EcoRI fragments were ligated into the replicative form of EcoRI-digested, dephosphorylated M13mp18. The EcoRI-Hind111fragments were ligated into similarly digested M13mp19. Ligated molecules were transformed in competent Escherichia coli LL308 cells. Cells from each transformation reaction were ...
... The EcoRI fragments were ligated into the replicative form of EcoRI-digested, dephosphorylated M13mp18. The EcoRI-Hind111fragments were ligated into similarly digested M13mp19. Ligated molecules were transformed in competent Escherichia coli LL308 cells. Cells from each transformation reaction were ...
Control of ribosome traffic by position-dependent
... to be 2/sec [11] and not rate-limiting. We study various values of the on-rate Ks. The on-rate Ks for lacZ for cells growing in a minimal media has been estimated to be about 0.9/sec [11]. Note that Ks is larger than the actual initiation rate, since the occlusion time of the start codon is finite. ...
... to be 2/sec [11] and not rate-limiting. We study various values of the on-rate Ks. The on-rate Ks for lacZ for cells growing in a minimal media has been estimated to be about 0.9/sec [11]. Note that Ks is larger than the actual initiation rate, since the occlusion time of the start codon is finite. ...
Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression
... off genes that are functional and thus contribute to the final phenotype. Examples of the effect of simple measures on methylation patterns during prenatal development are clear in their ability to change phenotypic expression. Based on this idea, if scientists could target methylation of specific g ...
... off genes that are functional and thus contribute to the final phenotype. Examples of the effect of simple measures on methylation patterns during prenatal development are clear in their ability to change phenotypic expression. Based on this idea, if scientists could target methylation of specific g ...
GENtle, a free multi-purpose molecular biology tool
... seamlessly integrating diverse applications for every-day lab use in a single package. It was designed for easy and intuitive use, while providing many powerful functions. This C++ application runs on multiple platforms, is optimized for performance, and includes database interfaces for easy sequenc ...
... seamlessly integrating diverse applications for every-day lab use in a single package. It was designed for easy and intuitive use, while providing many powerful functions. This C++ application runs on multiple platforms, is optimized for performance, and includes database interfaces for easy sequenc ...
Analysis and nucleotide sequence of an origin of DNA replication in
... stable maintenance in A. calcoaceticus. No rearrangements of the DNA or loss of plasmids are found in that organism, even in the absence of selective pressure, when this sequence is present. A further insertionai inactivation analysis creating lacZ transcriptional fusions suggests that the origin of ...
... stable maintenance in A. calcoaceticus. No rearrangements of the DNA or loss of plasmids are found in that organism, even in the absence of selective pressure, when this sequence is present. A further insertionai inactivation analysis creating lacZ transcriptional fusions suggests that the origin of ...
Frequent, independent transfers of a catabolic gene from bacteria to
... Indeed, gene duplications (followed by evolution of new functions) and other genomic rearrangements have shaped eukaryotic genomes [1]. However, genetic innovation can also result from the acquisition of exogenous genes by horizontal gene transfer (HGT). Prokaryotes adapt largely by HGT, and strains ...
... Indeed, gene duplications (followed by evolution of new functions) and other genomic rearrangements have shaped eukaryotic genomes [1]. However, genetic innovation can also result from the acquisition of exogenous genes by horizontal gene transfer (HGT). Prokaryotes adapt largely by HGT, and strains ...
DNA cytosine methylation in plant development
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
... (Bird, 2002). A striking difference in the cytosine methylation patterns in plants from those in animals is that although methylation is predominantly occurring at the CG dinucleotides in plants, it is not confined to these sites; instead, methylation also occurs at CHG (where H is A, C or T) and as ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
Proceedings as -file
... Comparison test: Comments from Duty Laboratory South Africa; Comments from Computing Laboratory The Netherlands; Discussion ISAG panels: Do they work well? How many laboratories are using these panels in the routine? Do we need changes? Standard / Reference samples? • Nomenclature: Is there a ne ...
... Comparison test: Comments from Duty Laboratory South Africa; Comments from Computing Laboratory The Netherlands; Discussion ISAG panels: Do they work well? How many laboratories are using these panels in the routine? Do we need changes? Standard / Reference samples? • Nomenclature: Is there a ne ...
Chpt12_RNAProcessing.doc
... (3) These two phosphoester transfers result in a joining of the two exons and excision of the intron (with the initiating G nucleotide attached to the 5' end.) (4) The excised intron is then circularized by attack of the 3'-OH of the last nucleotide of the intron on the phosphate between the 15th an ...
... (3) These two phosphoester transfers result in a joining of the two exons and excision of the intron (with the initiating G nucleotide attached to the 5' end.) (4) The excised intron is then circularized by attack of the 3'-OH of the last nucleotide of the intron on the phosphate between the 15th an ...
Plant Functional Genomics Plant Functional Genomics
... Large genomic DNA insert-containing libraries are essential for physical mapping, positional cloning, and genome sequencing of complex genomes. There are two principal large insert cloning systems that are constructed as yeast or bacterial artificial chromosomes (YACs and BACs, respectively). The YA ...
... Large genomic DNA insert-containing libraries are essential for physical mapping, positional cloning, and genome sequencing of complex genomes. There are two principal large insert cloning systems that are constructed as yeast or bacterial artificial chromosomes (YACs and BACs, respectively). The YA ...
The amphioxus hairy family: differential fate after duplication.
... Several representatives of the hairy family have been so far isolated from a range of protostome and vertebrate species. In protostomes such as Drosophila they have been implicated in segmentation, peripheral nervous system development, and in somatic sex determination. In Tribolium, only a role dur ...
... Several representatives of the hairy family have been so far isolated from a range of protostome and vertebrate species. In protostomes such as Drosophila they have been implicated in segmentation, peripheral nervous system development, and in somatic sex determination. In Tribolium, only a role dur ...
SVPaper050711
... Unexpectedly, in all cases the presence of SNPs in the microhomology region was correlated with the presence of the SV (Fig. 2b). The SNP elongates the microhomology, or, alternatively, the microhomology reflects a hypermutable state associated with break induced replication around the SV29. Howeve ...
... Unexpectedly, in all cases the presence of SNPs in the microhomology region was correlated with the presence of the SV (Fig. 2b). The SNP elongates the microhomology, or, alternatively, the microhomology reflects a hypermutable state associated with break induced replication around the SV29. Howeve ...
Detection and analysis of disease-associated single nucleotide
... Qingdao, China. 24-27 October 2014 ...
... Qingdao, China. 24-27 October 2014 ...
Genome-wide search for signatures of selection in three
... observed for farm animal species (Diamond, 2002; Toro and MäkiTanila, 2007; Flori et al., 2009). Brazilian sheep are descendants of animals imported during the colonization period and, since then, have been subject to systematic and non-systematic evolutionary processes. This has led to the developm ...
... observed for farm animal species (Diamond, 2002; Toro and MäkiTanila, 2007; Flori et al., 2009). Brazilian sheep are descendants of animals imported during the colonization period and, since then, have been subject to systematic and non-systematic evolutionary processes. This has led to the developm ...
PowerPoint Presentation - No Slide Title
... • Sorted by related sites • “Block” structure evident ...
... • Sorted by related sites • “Block” structure evident ...
Human genome
The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.