The S RNA segment of tomato spotted wilt virus has an ambisense
... 88 and terminates at an UAA stop codon at position 1481 (numbered from the 5' end of the viral strand), which corresponds to a protein of 465 amino acids and an Mr of 52.4K. The amino acid sequence of this putative protein does not contain hydropfiobic regions that might function as signal peptides ...
... 88 and terminates at an UAA stop codon at position 1481 (numbered from the 5' end of the viral strand), which corresponds to a protein of 465 amino acids and an Mr of 52.4K. The amino acid sequence of this putative protein does not contain hydropfiobic regions that might function as signal peptides ...
Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of
... that the organization of the mitochondrial rRNA genes is quite different from the rRNA genes of the chloroplast and nucleus in maize. In spite of the close eubacterial homology observed in the 18S sequence, the organization of rRNA genes is also different from the prokaryotic prototype. Earlier, Gra ...
... that the organization of the mitochondrial rRNA genes is quite different from the rRNA genes of the chloroplast and nucleus in maize. In spite of the close eubacterial homology observed in the 18S sequence, the organization of rRNA genes is also different from the prokaryotic prototype. Earlier, Gra ...
An Exploration of Command-Line BLAST
... Using BLAST to Search Watermelon Sequence Data: Hands-On Exercise Searching sequence data for similarities is one of the most common tasks in bioinformatics. In fact, identifying and quantifying sequence similarity (either nucleotide or amino acid) underlies many other types of sequence analyses. Se ...
... Using BLAST to Search Watermelon Sequence Data: Hands-On Exercise Searching sequence data for similarities is one of the most common tasks in bioinformatics. In fact, identifying and quantifying sequence similarity (either nucleotide or amino acid) underlies many other types of sequence analyses. Se ...
Localization of Three Genes in the Hook
... different from the one on which the other two genes were located (Fig. 2D). Thus, our study employed three genes located on two of the larger chromosomes of the hamster karyotype. This allowed us to compare the spatial distribution of two DNA sequences on different chromosomes, and of two genes loca ...
... different from the one on which the other two genes were located (Fig. 2D). Thus, our study employed three genes located on two of the larger chromosomes of the hamster karyotype. This allowed us to compare the spatial distribution of two DNA sequences on different chromosomes, and of two genes loca ...
XLibraryDisplay User Manual Ryan Stafford
... were run to determine background levels for the assay. It is ok to have multiple identical sample IDs with asterisks since they do not need to be uniquely associated with sequences. The program will check your data for consistency or other issues when you try to correlate sequences to activity data, ...
... were run to determine background levels for the assay. It is ok to have multiple identical sample IDs with asterisks since they do not need to be uniquely associated with sequences. The program will check your data for consistency or other issues when you try to correlate sequences to activity data, ...
CHAPTER 9
... are allowed to mate, and then the mating is interrupted at various time points. The interruption occurs by agitation of the solution in which the bacteria are found. This type of study is used to map the locations of genes. It is necessary to interrupt mating so that you can vary the time and obtain ...
... are allowed to mate, and then the mating is interrupted at various time points. The interruption occurs by agitation of the solution in which the bacteria are found. This type of study is used to map the locations of genes. It is necessary to interrupt mating so that you can vary the time and obtain ...
Presentation
... Figure 11.20 A Sliding DNA Clamp Increases the Efficiency of DNA Polymerization ...
... Figure 11.20 A Sliding DNA Clamp Increases the Efficiency of DNA Polymerization ...
Exercise 11 - Understanding the Output for a blastn Search
... sequence (Figure 3). Looking at the descriptions, it appears that our unknown D. yakuba genomic DNA has sequence homology to the gene legless in D. melanogaster. Comparing the descriptions for the top eight hits, we noticed that only the D. melanogaster hit has an accession number that begins with ‘ ...
... sequence (Figure 3). Looking at the descriptions, it appears that our unknown D. yakuba genomic DNA has sequence homology to the gene legless in D. melanogaster. Comparing the descriptions for the top eight hits, we noticed that only the D. melanogaster hit has an accession number that begins with ‘ ...
Evolution of Gene Order and Chromosome Number in
... are conserved. Analysis of gene order arrangements, chromosome numbers, and ribosomal RNA sequences suggests that genome duplication occurred before the divergence of the four species in Saccharomyces sensu stricto (all of which have 16 chromosomes), but after this lineage had diverged from Saccharo ...
... are conserved. Analysis of gene order arrangements, chromosome numbers, and ribosomal RNA sequences suggests that genome duplication occurred before the divergence of the four species in Saccharomyces sensu stricto (all of which have 16 chromosomes), but after this lineage had diverged from Saccharo ...
A familial inverted duplication/deletion of 2p25.1–25.3
... 2p: th 2p25.3 and 2p25.1 bands are depicted in green and red, respectively. The dots represent BAC clones RP11-90H11 (2p25.3, green dot) and RP111B18 (2p25.1, red dot). Double-colour FISH with BACs RP11-90H11 (green signal) and RP11-1B18 (red signal): the normal chromosome 2 (arrow) has two signals. ...
... 2p: th 2p25.3 and 2p25.1 bands are depicted in green and red, respectively. The dots represent BAC clones RP11-90H11 (2p25.3, green dot) and RP111B18 (2p25.1, red dot). Double-colour FISH with BACs RP11-90H11 (green signal) and RP11-1B18 (red signal): the normal chromosome 2 (arrow) has two signals. ...
Ontologies
... “An ontology is a controlled vocabulary of well defined terms with specified relationships between those terms, capable of interpretation by both humans and computers.” ...
... “An ontology is a controlled vocabulary of well defined terms with specified relationships between those terms, capable of interpretation by both humans and computers.” ...
use of genomic tools to discover the cause of
... Three sire families were identified as segregating for this trait. Genome wide linkage analysis using 104 microsatellite DNA markers was used to map the gene to ECA14 (LOD > 11.0). Four genes, namely SPARC, SLC36A1, SLC36A2 and SLC36A3, were selected from the region implicated by linkage and their e ...
... Three sire families were identified as segregating for this trait. Genome wide linkage analysis using 104 microsatellite DNA markers was used to map the gene to ECA14 (LOD > 11.0). Four genes, namely SPARC, SLC36A1, SLC36A2 and SLC36A3, were selected from the region implicated by linkage and their e ...
Attachment PDF Icon
... • A minimal continuous 16 bp match must exist for the 5' signature; the 3' signature must have a minimal continuous 14 bp match • Both 5' and 3' signatures must be present on the same chromosome ...
... • A minimal continuous 16 bp match must exist for the 5' signature; the 3' signature must have a minimal continuous 14 bp match • Both 5' and 3' signatures must be present on the same chromosome ...
Supplement: Sequence analysis of GMR-dRetMEN2B
... AE003683 maps to different sites within AE003683. There may be 2 inserts, one in 1st intron of Dh, the other 92 bp upstream of Crc, or a single P that induced a 10.4 kb deletion to remove Scm, SpdS, the 1st exon of Dh ...
... AE003683 maps to different sites within AE003683. There may be 2 inserts, one in 1st intron of Dh, the other 92 bp upstream of Crc, or a single P that induced a 10.4 kb deletion to remove Scm, SpdS, the 1st exon of Dh ...
Sample Chapter 3 (PDF, 30 Pages
... thymine, cytosine, and guanine, identified by the letters A, T, C, and G—are arranged in a particular order: for example, ACGTCTCTATA. . . . This sequence may contain thousands or even tens of thousands of “letters,” which together constitute a code for the synthesis of one of the many proteins tha ...
... thymine, cytosine, and guanine, identified by the letters A, T, C, and G—are arranged in a particular order: for example, ACGTCTCTATA. . . . This sequence may contain thousands or even tens of thousands of “letters,” which together constitute a code for the synthesis of one of the many proteins tha ...
(2013). Nothing in genetics makes sense except in light of genomic
... in ZZ/ZW species. The same logic would apply to a similar mutation in a vertically transmitted cytoplasmic endosymbiont (e.g., Wolbachia) that is propagated only through the matriline. Next, suppose that A and B are two alleles at a single locus. If A increases because (a) it was favored by selectio ...
... in ZZ/ZW species. The same logic would apply to a similar mutation in a vertically transmitted cytoplasmic endosymbiont (e.g., Wolbachia) that is propagated only through the matriline. Next, suppose that A and B are two alleles at a single locus. If A increases because (a) it was favored by selectio ...
Chapter 14: Gene Transcription and RNA Modification
... and form an open complex. However, unlike the bacterial systems, eukaryotic systems employ three different RNA polymerases to transcribe the various categories of genes. These include RNA polymerase II, which transcribes mRNAs. Moreover, eukaryotes utilize a variety of proteins, called transcription ...
... and form an open complex. However, unlike the bacterial systems, eukaryotic systems employ three different RNA polymerases to transcribe the various categories of genes. These include RNA polymerase II, which transcribes mRNAs. Moreover, eukaryotes utilize a variety of proteins, called transcription ...
1 Glossary 5` overhang- Restriction enzymes that cleave the DNA
... Anabolic – The process or the enzymes that build (i.e. synthesize) a substrate rather than break a substrate down. An example is the amino acid tryptophan, whose synthesis requires the action of four different enzymes encoded by five different genes. ...
... Anabolic – The process or the enzymes that build (i.e. synthesize) a substrate rather than break a substrate down. An example is the amino acid tryptophan, whose synthesis requires the action of four different enzymes encoded by five different genes. ...
Department of Biomedical Informatics
... most common form of genetic variation. As the name suggests, each SNP is a difference in a single nucleotide (A,T,C,or G) of an individual's DNA sequence, such as having AAGG instead of ATGG. There may be from 1 to 10 million SNPs in the entire human genome, but perhaps only a few thousand relate to ...
... most common form of genetic variation. As the name suggests, each SNP is a difference in a single nucleotide (A,T,C,or G) of an individual's DNA sequence, such as having AAGG instead of ATGG. There may be from 1 to 10 million SNPs in the entire human genome, but perhaps only a few thousand relate to ...
Microsoft Word Document
... Anabolic – The process or the enzymes that build (i.e. synthesize) a substrate rather than break a substrate down. An example is the amino acid tryptophan, whose synthesis requires the action of four different enzymes encoded by five different genes. ...
... Anabolic – The process or the enzymes that build (i.e. synthesize) a substrate rather than break a substrate down. An example is the amino acid tryptophan, whose synthesis requires the action of four different enzymes encoded by five different genes. ...
Amphioxus
... intestinalis [19]. Like vertebrate MTs, CiMT-1 is composed of three exons and two introns, but only two of its exons comprise coding stretches, so that the encoded MT peptide is the shortest currently known in deuterostomes. The corresponding protein still retains the characteristic 30% cysteine con ...
... intestinalis [19]. Like vertebrate MTs, CiMT-1 is composed of three exons and two introns, but only two of its exons comprise coding stretches, so that the encoded MT peptide is the shortest currently known in deuterostomes. The corresponding protein still retains the characteristic 30% cysteine con ...
Trans-HHS Workshop: Diet, DNA Methylation
... silencing are quite convincing. In vitro methylation of promoter-reporter constructs inhibits their subsequent expression in transfected cells (50). Demethylation by 5-azadeoxycytidine, a DNA methyltransferase inhibitor, leads to re-expression of previously methylated genes (51). Homozygous embryos ...
... silencing are quite convincing. In vitro methylation of promoter-reporter constructs inhibits their subsequent expression in transfected cells (50). Demethylation by 5-azadeoxycytidine, a DNA methyltransferase inhibitor, leads to re-expression of previously methylated genes (51). Homozygous embryos ...
Translation
... • RNA polyadenylation: repeated adenine nucleotides (100-200) are bound to the 3´end (poly-A end). These two modifications increase the stability of mRNA. RNA splicing: noncoding sequenses (introns) are removed from primary transcript and codding sequenses (exons) are joined in given order. [FIG.] [ ...
... • RNA polyadenylation: repeated adenine nucleotides (100-200) are bound to the 3´end (poly-A end). These two modifications increase the stability of mRNA. RNA splicing: noncoding sequenses (introns) are removed from primary transcript and codding sequenses (exons) are joined in given order. [FIG.] [ ...
Translation
... • RNA polyadenylation: repeated adenine nucleotides (100-200) are bound to the 3´end (poly-A end). These two modifications increase the stability of mRNA. RNA splicing: noncoding sequenses (introns) are removed from primary transcript and codding sequenses (exons) are joined in given order. [FIG.] [ ...
... • RNA polyadenylation: repeated adenine nucleotides (100-200) are bound to the 3´end (poly-A end). These two modifications increase the stability of mRNA. RNA splicing: noncoding sequenses (introns) are removed from primary transcript and codding sequenses (exons) are joined in given order. [FIG.] [ ...
WORKING WTH THE FIGURES
... many chromosomes would be in the abnormal product? Answer: Colchicine prevents migration of chromatids, and the abnormal product of such treatment would keep all the chromatids (2n = 18) in one cell. ...
... many chromosomes would be in the abnormal product? Answer: Colchicine prevents migration of chromatids, and the abnormal product of such treatment would keep all the chromatids (2n = 18) in one cell. ...
Human genome
The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.