RELIC – A bioinformatics server for combinatorial
... in the population. Statistical dissection of the regular sequence biases with custom-designed algorithms has been used by our group to identify inclusion-limiting steps during library construction [1]. These results, as well as data from others, demonstrate that alternation of affinity selection wit ...
... in the population. Statistical dissection of the regular sequence biases with custom-designed algorithms has been used by our group to identify inclusion-limiting steps during library construction [1]. These results, as well as data from others, demonstrate that alternation of affinity selection wit ...
Medicinal Chemistry Reviews
... there is no access to unnatural side chains, there is the substantial importance of ease of creation of the mutant protein at essentially any scale necessary. Modern synthesis of DNA oligonucleotides can provide 100-mers or more, and PCRbased methods make it simple to stitch together genes of essent ...
... there is no access to unnatural side chains, there is the substantial importance of ease of creation of the mutant protein at essentially any scale necessary. Modern synthesis of DNA oligonucleotides can provide 100-mers or more, and PCRbased methods make it simple to stitch together genes of essent ...
Structure of a Plasmodium yoelii gene
... for 2h at 42 °C. Hybridizations were done under the same conditions using a 1 ;
... for 2h at 42 °C. Hybridizations were done under the same conditions using a 1 ;
Towards the construction of Escherichia coli cell
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
... Cell-free protein systems are described as the in vitro expression of recombinant proteins without the use of living cells. This approach uses a cell lysate containing a wide array of biological and chemical components for transcription, translation, protein folding, and energy metabolism; all requi ...
Document
... Protein Structure: primary (1°) structure: the amino acid sequence secondary (2°): frequently occurring substructures or folds tertiary (3°): three-dimensional arrangement of all atoms in a single polypeptide chain quaternary (4°): overall organization of non-covalently linked subunits of a function ...
... Protein Structure: primary (1°) structure: the amino acid sequence secondary (2°): frequently occurring substructures or folds tertiary (3°): three-dimensional arrangement of all atoms in a single polypeptide chain quaternary (4°): overall organization of non-covalently linked subunits of a function ...
Effects of Aging on Activities of Mitochondrial Electron Transport
... The aging heart is characterized by many structural, physiological and biochemical changes. Numerous studies have suggested that age-associated deterioration in heart function can be related to oxidative damage by reactive oxygen species (ROS) produced during mitochondrial oxidative phosphorylation. ...
... The aging heart is characterized by many structural, physiological and biochemical changes. Numerous studies have suggested that age-associated deterioration in heart function can be related to oxidative damage by reactive oxygen species (ROS) produced during mitochondrial oxidative phosphorylation. ...
Inquiry into Life Twelfth Edition
... Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
... Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. ...
The topology of the proton translocating F0 component of the ATP
... Protease sensitivity of the F0 sector subunits a, b and c F 1-depleted membranes were chosen rather than the isolated ATP synthase complex to avoid possible artefacts during reconstitution of the complex. In wild-type membranes the ATP synthase constituted - 1OOJo of the protein mass. Thus, it was n ...
... Protease sensitivity of the F0 sector subunits a, b and c F 1-depleted membranes were chosen rather than the isolated ATP synthase complex to avoid possible artefacts during reconstitution of the complex. In wild-type membranes the ATP synthase constituted - 1OOJo of the protein mass. Thus, it was n ...
Practical: Comparative modelling of Gdh using MODELLER
... name that you gave your top file in part 2. Modeller, if run correctly, will run for a while as it models the sequence then does the minimization steps. The initial output is your 3 or 4 letter name for your protein followed by ".ini" and the actual models are followed by "B9999??" where ?? is the n ...
... name that you gave your top file in part 2. Modeller, if run correctly, will run for a while as it models the sequence then does the minimization steps. The initial output is your 3 or 4 letter name for your protein followed by ".ini" and the actual models are followed by "B9999??" where ?? is the n ...
The role of calcium and other ions in sorting and delivery in the late
... endosomes. The formation of luminal vesicles and the process of sorting membrane proteins destined for degradation into these vesicles have recently received much attention (reviewed in [9]). In yeast, genetic screens identified genes encoding 17 soluble Class E Vps (vacuolar protein sorting) protei ...
... endosomes. The formation of luminal vesicles and the process of sorting membrane proteins destined for degradation into these vesicles have recently received much attention (reviewed in [9]). In yeast, genetic screens identified genes encoding 17 soluble Class E Vps (vacuolar protein sorting) protei ...
Modeling of Protein-Small Molecule Complexes
... is -86.7421 Kcal/mol. The difference between the two is 61.4421 Kcal/mol favoring the energy minimized conformation. In summary, the “Charge/Charge” interaction made the greatest difference between the two conformations. This is due to the interaction of the nitrogen group with the more negative ca ...
... is -86.7421 Kcal/mol. The difference between the two is 61.4421 Kcal/mol favoring the energy minimized conformation. In summary, the “Charge/Charge” interaction made the greatest difference between the two conformations. This is due to the interaction of the nitrogen group with the more negative ca ...
The Amino Acid Sequence Contains Leucine-Rich
... sequence of Th2 that provided one of the two sequences. The cause of unexpected cleavage between Asp-Ser within the Th2 peptide is unknown. The amino terminus of T h l was blocked, clearly indicating that GPVfl (69 Kd fragment of GPV) was derived from the N-terminal region of GPV, and the thrombin c ...
... sequence of Th2 that provided one of the two sequences. The cause of unexpected cleavage between Asp-Ser within the Th2 peptide is unknown. The amino terminus of T h l was blocked, clearly indicating that GPVfl (69 Kd fragment of GPV) was derived from the N-terminal region of GPV, and the thrombin c ...
Steroid hormone receptor homologs in development
... requires a mechanistic explanation for how a single transcription factor, when bound to its ligand, acts as a sequence-specific, positive and negative regulatory factor and a delineation of the unique domains in the receptor dedicated to each phenomenon. Through cotransfection studies using the 'cis ...
... requires a mechanistic explanation for how a single transcription factor, when bound to its ligand, acts as a sequence-specific, positive and negative regulatory factor and a delineation of the unique domains in the receptor dedicated to each phenomenon. Through cotransfection studies using the 'cis ...
Sulfur Metabolism in Escherichia coli and Related Bacteria: Facts
... following (Figure 2) (Greene, 1996). Biosynthesis of methionine originates from the homoserine pathway (which branches to lysine via diaminopimelate, an essential component of mureine, to threonine, and to isoleucine), starting from the synthesis of an activated derivative, Osuccinylhomoserine. This ...
... following (Figure 2) (Greene, 1996). Biosynthesis of methionine originates from the homoserine pathway (which branches to lysine via diaminopimelate, an essential component of mureine, to threonine, and to isoleucine), starting from the synthesis of an activated derivative, Osuccinylhomoserine. This ...
Glycogen Metabolism Gluconeogenesis
... • In the “resting” state, Gα is bound to the Gβ-Gγ dimer. Gα contains the nucleotide binding site, holding GDP in the inactive form, and is the “warhead” of the G protein. At least 20 different forms of Ga exist in mammalian cells. • Binding of the extracellular signal by the GPCR causes it to under ...
... • In the “resting” state, Gα is bound to the Gβ-Gγ dimer. Gα contains the nucleotide binding site, holding GDP in the inactive form, and is the “warhead” of the G protein. At least 20 different forms of Ga exist in mammalian cells. • Binding of the extracellular signal by the GPCR causes it to under ...
Hacking nature: genetic tools for reprograming enzymes
... amino acid is encoded by a single codon, making available coding ...
... amino acid is encoded by a single codon, making available coding ...
The Effect of Actidione and other Antifungal Agents on Nucleic Acid
... the initial rate of uptake was decreased and after 60 min. incorporation had almost completely stopped. The results presented so far lead to the conclusion that concentrations of actidione equal to or greater than the minimum growth inhibitory concentration completely inhibit DNA synthesis, whereas ...
... the initial rate of uptake was decreased and after 60 min. incorporation had almost completely stopped. The results presented so far lead to the conclusion that concentrations of actidione equal to or greater than the minimum growth inhibitory concentration completely inhibit DNA synthesis, whereas ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.