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Metabolic adaptation of Mycobacterium avium subsp
Metabolic adaptation of Mycobacterium avium subsp

... indicate that MTB uses various pathways that mediate oxidative stress resistance and cell wall synthesis to evade the host immune responses and adapt to its intracellular lifestyle in the macrophage (Mukhopadhyay et al., 2012). A better understanding of mycobacterial metabolism in the infected host ...
BEL β-trefoil: A novel lectin with antineoplastic properties in king
BEL β-trefoil: A novel lectin with antineoplastic properties in king

... (SDS–PAGE) when the sample was not boiled before running the electrophoresis and a single band with the molecular weight of the monomer if the sample was boiled instead (data not shown). This experiment indicated that the dimer was stable even in the presence of SDS and that exposure to high tempera ...
The nucleosome
The nucleosome

...  Positive control elements recognized by activators ...
Cell-Specific Localization of Glucose Transporter Proteins in
Cell-Specific Localization of Glucose Transporter Proteins in

... in many tissues is dependent on a sustained supply of glucose (1). The lung, a vital organ that performs the function of oxygenation and defense against a host of factors, uses glucose for cellular oxidation (2), growth, development (3), and biological functions such as surfactant synthesis and secr ...
Biological Networks Underlying Abiotic Stress Tolerance in
Biological Networks Underlying Abiotic Stress Tolerance in

... proteome level, especially when using 2DE based approaches, alterations in protein abundance are scarcely detected due to a relatively low abundance of signaling proteins with respect to other cellular proteins and due to the rapidity of their changes during an alarm phase of stress response. Howeve ...
MIBCB Syllabus
MIBCB Syllabus

... possess fundamental biochemical knowledge and have the ability to use this knowledge most appropriately as applied to clinical requirements, i.e. diagnosis of disease and planning and monitoring of therapy. Apart from providing a competent laboratory service, the clinical chemist must be able to fun ...
Membrane Transport - Bioenergetics and Cell Metabolism
Membrane Transport - Bioenergetics and Cell Metabolism

... Mechanisms by which cells solve this problem include: ...
Chapter 11 Vitamins and proteins
Chapter 11 Vitamins and proteins

... to manufacture protein may be obtained. Except for glycine, amino acids are enantiomers due to their chiral centres, meaning that they are either the l or d form, according to the arrangement of groups around the chiral carbon atom. The molecule synthesised from naturally occurring (+)-glyceraldehyd ...
The Effects of Whey Protein and Leucine Supplementation
The Effects of Whey Protein and Leucine Supplementation

... Resistance training has been shown to increase strength (1,2), muscular endurance (3,4), body weight (2) and hypertrophy (5,6), as well as result in favorable changes in body composition including decreases in percent body fat (%fat) and fat weight (FW), and increases in fat-free weight (FFW) (2, 7) ...
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cissn study guide - Science Driven Nutrition

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BioInformatics at FSU
BioInformatics at FSU

... in order of increasing power and complexity: The sequence is in a local GCG format single sequence file in your UNIX account. (GCG Reformat and all From & To programs) The sequence is in a local GCG database in which case you ‘point’ to it by using any of the GCG database logical names. A colon, “:, ...
Write on zinc fingers
Write on zinc fingers

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Jacob Corn
Jacob Corn

... 1993). Therefore, it seemed the only major difference between infectious prions and normal prions was secondary and tertiary structure. The infectious protein hypothesis gained significant respect when circular dichroism and Fourier-transform infrared spectroscopy analyses of PrPC and PrPSc indicate ...
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES
STRUCTURAL INSIGHTS INTO NOVEL MICROBIAL METALLOENZYMES

... Metalloproteins represent a large portion of the total proteome. When bound to a protein a metal ion influences both protein stability and function through structural, catalytic or regulatory roles. Discovery of a metal ion cofactor presents new insight into both the structural and functional aspect ...
Topological studies suggest that the pathway of the protons through
Topological studies suggest that the pathway of the protons through

... obtained in ATP synthases from either organism. In E. coli~ however, this stretch was labeled in an SDS solution. The absence of Iabel in the native protein c (proteolipid) was thus not due to a low reactivity of the amino acids in this segment. These observations are interpreted to suggest that thi ...
Protein structural class prediction using predicted secondary
Protein structural class prediction using predicted secondary

... In the fast paced scientific world, the amount of biological data is already vast and continues to grow rapidly. These data are handled by applications developed in the field of bioinformatics. In this field of science, the discipline of biology, computer science, and information technology merge to ...
The ins and outs of sphingolipid synthesis
The ins and outs of sphingolipid synthesis

Anti-Human Ciliary Neurotrophic Factor (CNTF) (C3960)
Anti-Human Ciliary Neurotrophic Factor (CNTF) (C3960)

Changes in chemical composition in male turkeys
Changes in chemical composition in male turkeys

... significantly from unity. The fat-free FFB contained on average 71.6, 4.2, and 24.2% water, ash, and protein, respectively. Feathers were mostly composed of protein (Table 1) and the protein content did not change with feather mass (Table 2), resulting in an average protein content of 88.6% in feath ...
Regulation of the heat stress response in Arabidopsis by
Regulation of the heat stress response in Arabidopsis by

Reducing Rice Seed Storage Protein Accumulation Leads to
Reducing Rice Seed Storage Protein Accumulation Leads to

... germinating seedlings. We have generated glutelin, globulin and prolamin knock-down lines and have examined their effects on seed quality. A reduction of one or a few SSP(s) was compensated for by increases in other SSPs at both the mRNA and protein levels. Especially, reduction of glutelins or sufu ...
Slide 1
Slide 1

...  Groups (families/clusters) of proteins based on…  Overall sequence similarity.  Local sequence similarity.  Presence / absence of specific features (active site, signal peptides… ...
Biochemistry Spring 2015 Exam III Name: Points
Biochemistry Spring 2015 Exam III Name: Points

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Phospholipid synthesis in Borrelia burgdorferi: BB0249 and BB0721
Phospholipid synthesis in Borrelia burgdorferi: BB0249 and BB0721

... protocols (Ames, 1968) and TLC of the extracts was performed. Staining of the TLC plates confirmed that PC and PG are the major phospholipids in the B. burgdorferi membrane (data not shown) and suggested that this method was a reliable method for examining B. burgdorferi membrane phospholipids. We t ...
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine

... stretch of codons, which after translation give rise to different amino acids that may influence the properties of the protein. Cassette-mutagenesis results in the repla~ment of a particular DNA sequence with a synthetic DNA fragment containing the desired mutation with almost 1000A.efficiency (Old ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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