Crystal structure of a membrane-bound l-amino acid
... deamination of L-amino acids to a-keto acids. They are widely distributed in eukaryotic and prokaryotic organisms, and exhibit diverse substrate specificity, post-translational modifications and cellular localization. While LAAOs isolated from snake venom have been extensively characterized, the str ...
... deamination of L-amino acids to a-keto acids. They are widely distributed in eukaryotic and prokaryotic organisms, and exhibit diverse substrate specificity, post-translational modifications and cellular localization. While LAAOs isolated from snake venom have been extensively characterized, the str ...
PDF - FEMS Microbiology Letters
... tated in the database as glutamate decarboxylase and related PLP-dependent proteins (ZP_00287527) and today as pyridoxal-dependent decarboxylase (ZP_00602894), and showed 85% identity to Ent. faecalis tyrosine decarboxylase. The gene coding for this putative decarboxylase was expressed in Escherichi ...
... tated in the database as glutamate decarboxylase and related PLP-dependent proteins (ZP_00287527) and today as pyridoxal-dependent decarboxylase (ZP_00602894), and showed 85% identity to Ent. faecalis tyrosine decarboxylase. The gene coding for this putative decarboxylase was expressed in Escherichi ...
video slide - Manchester Township School District
... • (16)In addition to primary structure, physical and chemical conditions can affect conformation • Alternations in pH, salt concentration, temperature, or other environmental factors can cause a protein to unravel • (17)This loss of a protein’s native conformation is called denaturation • A denature ...
... • (16)In addition to primary structure, physical and chemical conditions can affect conformation • Alternations in pH, salt concentration, temperature, or other environmental factors can cause a protein to unravel • (17)This loss of a protein’s native conformation is called denaturation • A denature ...
Plasmodium falciparum enolase - Tata Institute of Fundamental
... targets and vaccine candidates [5–8]. P. falciparum enolase (Pfen) (EC 4.2.1.11), the dehydrating glycolytic metalloenzyme that catalyzes the inter conversion of 2-phosphoglyceric acid (2-PGA) and phosphoenolpyruvate (PEP), has not yet been characterized. Enolases are highly conserved across species ...
... targets and vaccine candidates [5–8]. P. falciparum enolase (Pfen) (EC 4.2.1.11), the dehydrating glycolytic metalloenzyme that catalyzes the inter conversion of 2-phosphoglyceric acid (2-PGA) and phosphoenolpyruvate (PEP), has not yet been characterized. Enolases are highly conserved across species ...
Nutrition to Support Recovery from Endurance Exercise: Optimal
... adenosine triphosphate production), but their absolute oxidation can be increased several-fold with the increased energy demands of endurance-type exercise; this oxidation represents a net loss of amino acids from the free pool within muscle. In addition, endurance exercise is a major stimulus to re ...
... adenosine triphosphate production), but their absolute oxidation can be increased several-fold with the increased energy demands of endurance-type exercise; this oxidation represents a net loss of amino acids from the free pool within muscle. In addition, endurance exercise is a major stimulus to re ...
The Additive Screen MD1-11
... There are 24 unique reagents supplied in the kit. The concentrations of the reagents have been designed to allow minimum dilution of the sample drop, and are sufficiently concentrated that vapour diffusion experiments can be carried out in as little as a 2.5µl drop. All additives have to be added to ...
... There are 24 unique reagents supplied in the kit. The concentrations of the reagents have been designed to allow minimum dilution of the sample drop, and are sufficiently concentrated that vapour diffusion experiments can be carried out in as little as a 2.5µl drop. All additives have to be added to ...
Divergent Evolution of Function in the ROK Sugar
... transformed cells were pelleted by centrifugation at 3000g, washed twice with 1 mL of M9 minimal medium, and plated on M9 minimal plates containing glucose (0.005% w/v), ampicillin (150 µg/mL), chloramphenicol (25 µg/mL), and kanamycin (40 µg/mL). Selection plates were also supplemented with IPTG at ...
... transformed cells were pelleted by centrifugation at 3000g, washed twice with 1 mL of M9 minimal medium, and plated on M9 minimal plates containing glucose (0.005% w/v), ampicillin (150 µg/mL), chloramphenicol (25 µg/mL), and kanamycin (40 µg/mL). Selection plates were also supplemented with IPTG at ...
Comparison of Amino Acid Sequences of Halloween Genes in
... PCR: The PCRs were carried out using Taq DNA polymerase (Fermentas) with the following general conditions: 15 - 20 ng of genomic DNA was used in a 20 μl reaction with 5 U/μl of Taq DNA polymerase, 2.5 mM each dNTP mix and 10 Pm/μl l of each primer. For Spo and Phm, initial denaturation was at 94˚C f ...
... PCR: The PCRs were carried out using Taq DNA polymerase (Fermentas) with the following general conditions: 15 - 20 ng of genomic DNA was used in a 20 μl reaction with 5 U/μl of Taq DNA polymerase, 2.5 mM each dNTP mix and 10 Pm/μl l of each primer. For Spo and Phm, initial denaturation was at 94˚C f ...
Electrostatics
... Active sites or shifted pKas? Structural (de)stabilization? PH domain comparison: similar fold, similar electrostatics, different sequence. Blomberg N, et al. Proteins 37, 379-877 (1999). ...
... Active sites or shifted pKas? Structural (de)stabilization? PH domain comparison: similar fold, similar electrostatics, different sequence. Blomberg N, et al. Proteins 37, 379-877 (1999). ...
Alfalfa Cyclins: Differential Expression during the Cell
... isolated and rescreened according to the method of Hanks et al. (1988) at a temperature of 58OC in order to distinguish between false positives with a fit of less than 16 nucleotides. Only one colony was found to hybridize under these conditions to the I-L-V(I)-D-W-L-Voligonucleotide. DNA was prepar ...
... isolated and rescreened according to the method of Hanks et al. (1988) at a temperature of 58OC in order to distinguish between false positives with a fit of less than 16 nucleotides. Only one colony was found to hybridize under these conditions to the I-L-V(I)-D-W-L-Voligonucleotide. DNA was prepar ...
MB206_fhs_lnt_001.1_AT_May09
... The operon model of prokaryotic gene regulation was proposed by Fancois Jacob and Jacques Monod. The lac operon is an operon required for the transport and metabolism of lactose in Escherichia coli and some other enteric bacteria. It consists of three adjacent structural genes, a promoter, a termin ...
... The operon model of prokaryotic gene regulation was proposed by Fancois Jacob and Jacques Monod. The lac operon is an operon required for the transport and metabolism of lactose in Escherichia coli and some other enteric bacteria. It consists of three adjacent structural genes, a promoter, a termin ...
SACE2 Chemistry Workbook Sample Chapter
... Hydrogenation is a process that changes unsaturated fatty acids and triglycerides into saturated compounds. The process was first used in the 1890s by American chemist James F Boyce Senior. Boyce mixed powdered nickel with a heated sample of cottonseed oil before bubbling hydrogen gas into the mixtu ...
... Hydrogenation is a process that changes unsaturated fatty acids and triglycerides into saturated compounds. The process was first used in the 1890s by American chemist James F Boyce Senior. Boyce mixed powdered nickel with a heated sample of cottonseed oil before bubbling hydrogen gas into the mixtu ...
Analysis of Protein Phosphorylation Using Mass Spectrometry
... enriched proteins are still too complicated for phosphorylation analysis after enzymatic digestion. It can be ameliorated by coupling with a chromatographic enrichment. IMAC or TiO2 has become a common tool for phosphopeptide enrichment. No experimental evidence has shown inconsistencies in binding ...
... enriched proteins are still too complicated for phosphorylation analysis after enzymatic digestion. It can be ameliorated by coupling with a chromatographic enrichment. IMAC or TiO2 has become a common tool for phosphopeptide enrichment. No experimental evidence has shown inconsistencies in binding ...
PG1005 Lecture 18 Translation
... The Genetic Code • Triplet codes (codons) specify particular amino acids • Due to a complimentary anticodon found on tRNA charged with specific amino acids N.B. • Redundancy • Start and Stop Codons Redundancy most frequent at third base (relevant later) ...
... The Genetic Code • Triplet codes (codons) specify particular amino acids • Due to a complimentary anticodon found on tRNA charged with specific amino acids N.B. • Redundancy • Start and Stop Codons Redundancy most frequent at third base (relevant later) ...
novel nucleotide carrier proteins of Protochlamydia
... general the obligate intracellular lifestyle correlates with a drastically reduced genome of the bacteria, typically showing defects in essential biosynthetic pathways (Moran, 2002) and compensatory transport mechanisms for the import of key metabolites from the host cytosol (Moulder, 1991). For exa ...
... general the obligate intracellular lifestyle correlates with a drastically reduced genome of the bacteria, typically showing defects in essential biosynthetic pathways (Moran, 2002) and compensatory transport mechanisms for the import of key metabolites from the host cytosol (Moulder, 1991). For exa ...
SUPPLEMENTAL MATERIALS AND METHODS Ceramide and
... Sphingolipid enriched fractions were prepared from 100 flies in each batch and d14 sphingoid base containing ceramides and ceramide 1-phophate were estimated by mass spectrometry (Xevo TQ-S, Waters) following previously published protocols [1,2]. ...
... Sphingolipid enriched fractions were prepared from 100 flies in each batch and d14 sphingoid base containing ceramides and ceramide 1-phophate were estimated by mass spectrometry (Xevo TQ-S, Waters) following previously published protocols [1,2]. ...
Valine Mydrogenase from Streptmzyces fiadipe
... Valine dehydrogenase (VDH ; EC 1.4.1.8) activity was detected in a cell-free extract of Streptomycesfiadk in which it was thought to be a mgulatory enzyme involved in biosynthesis of n-butyrate, a buiiding unit of the oligoketideantibiotictylosin ( b u r a et al., 1983). Inhibition of VDH synthesisa ...
... Valine dehydrogenase (VDH ; EC 1.4.1.8) activity was detected in a cell-free extract of Streptomycesfiadk in which it was thought to be a mgulatory enzyme involved in biosynthesis of n-butyrate, a buiiding unit of the oligoketideantibiotictylosin ( b u r a et al., 1983). Inhibition of VDH synthesisa ...
Syllabus for GUTS lecture on Amino Acids
... zero charge at physiological pH and that do not participate in hydrogen or ionic bonds. Typically these side chains are hydrocarbon-rich, are therefore hydrophobic, and in most proteins are more often found in the interior where they are protected from water. In membrane-bound proteins, the opposite ...
... zero charge at physiological pH and that do not participate in hydrogen or ionic bonds. Typically these side chains are hydrocarbon-rich, are therefore hydrophobic, and in most proteins are more often found in the interior where they are protected from water. In membrane-bound proteins, the opposite ...
Feeney_ku_0099D_12934_DATA_1 - KU ScholarWorks
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
... Purpose: Oxidative post-translational modification of protein-bound tyrosine residues can have a significant impact on protein structure and function and thus may be important to physiological and pathological processes. Oxidative stress has been correlated with biological aging and many disease sta ...
Predicting DNA-binding sites of proteins from amino acid sequence
... Protein-DNA interactions play a pivotal role in gene regulation. The ability to identify amino acid residues that are responsible for the specificity and affinity of the interactions can significantly improve our understanding of macromolecular functions and contribute to advances in drug discovery ...
... Protein-DNA interactions play a pivotal role in gene regulation. The ability to identify amino acid residues that are responsible for the specificity and affinity of the interactions can significantly improve our understanding of macromolecular functions and contribute to advances in drug discovery ...
Amino Acids and Proteins Amino Acid Compound
... comparison with the migration of DNP-derivative standards allows for the identification of the N-terminal amino acid. Dansyl chloride: Like DNF, dansyl chloride reacts with the N-terminal residue under alkaline conditions. Analysis of the modified amino acids is carried out similarly to the Sanger ...
... comparison with the migration of DNP-derivative standards allows for the identification of the N-terminal amino acid. Dansyl chloride: Like DNF, dansyl chloride reacts with the N-terminal residue under alkaline conditions. Analysis of the modified amino acids is carried out similarly to the Sanger ...
Chapter 10
... translated into amino acid sequences • The flow of information from gene to protein is based on a triplet code. • The genetic instructions for the amino acid sequence of a polypeptide chain are written in DNA and RNA as a series of nonoverlapping three-base “words” called ...
... translated into amino acid sequences • The flow of information from gene to protein is based on a triplet code. • The genetic instructions for the amino acid sequence of a polypeptide chain are written in DNA and RNA as a series of nonoverlapping three-base “words” called ...
View/Open - VUW research archive - Victoria University of Wellington
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
... Conditions for extraction and two-dimensional electrophoresis of proteins were established. One hundred and seventy nine proteins were identified by MALDI mass spectrometry of tryptic digests of protein spots excised from Coomassie stained gels. All of the enzymes for conversion of glucose to ethano ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.