European Journal of Biochemistry
... The adsorption constant of the irreversible adsorption of the bacteriophage K3 to Escherichia coli K12 bacteria is strongly dependent on the incubation temperature. Two inflection points are observed in an Arrhenius plot. For cells grown at 37 "C the inflection points are found at 20 "C and 28 "C wh ...
... The adsorption constant of the irreversible adsorption of the bacteriophage K3 to Escherichia coli K12 bacteria is strongly dependent on the incubation temperature. Two inflection points are observed in an Arrhenius plot. For cells grown at 37 "C the inflection points are found at 20 "C and 28 "C wh ...
Immunoblot Detection of Proteins That Contain Cysteine
... that Srx physically associates with the 2-Cys Prxs but not with other sulfinic proteins. In addition to peroxidase activity, 2-Cys Prxs function as molecular chaperones. While the hyperoxidation of the activesite cysteine results in inactivation of peroxides activity, the chaperone function is enhan ...
... that Srx physically associates with the 2-Cys Prxs but not with other sulfinic proteins. In addition to peroxidase activity, 2-Cys Prxs function as molecular chaperones. While the hyperoxidation of the activesite cysteine results in inactivation of peroxides activity, the chaperone function is enhan ...
Phylogenetic relationship of phototrophic purple sulfur bacteria
... 0.5 or 1 ml of acetate solution (NH4-acetate/Mg-acetate; 2.5 g each/100 ml). The final acetate concentration of the medium was 0.05%. Depending on the species, NaCl was added to the medium to a final concentration of 0–10% (medium for marine isolates contained 2% NaCl). After inoculation, the bottle ...
... 0.5 or 1 ml of acetate solution (NH4-acetate/Mg-acetate; 2.5 g each/100 ml). The final acetate concentration of the medium was 0.05%. Depending on the species, NaCl was added to the medium to a final concentration of 0–10% (medium for marine isolates contained 2% NaCl). After inoculation, the bottle ...
Rooting the Ribosomal Tree of Life Research article
... ribosomal RNAs) have remained unrooted. Individual core ribosomal proteins are short in length, each containing few phylogenetically informative positions. As such, although universal phylogenies generated from alignments of individual ribosomal proteins generally do not show significant conflict, t ...
... ribosomal RNAs) have remained unrooted. Individual core ribosomal proteins are short in length, each containing few phylogenetically informative positions. As such, although universal phylogenies generated from alignments of individual ribosomal proteins generally do not show significant conflict, t ...
Automated extraction of mutation data from the literature: application
... heterogeneous data from across a variety of electronic and literature sources in order to draw new inferences about the target protein families. Results: We present a computational method that identifies and extracts mutation data from the scientific literature. We focused on the extraction of singl ...
... heterogeneous data from across a variety of electronic and literature sources in order to draw new inferences about the target protein families. Results: We present a computational method that identifies and extracts mutation data from the scientific literature. We focused on the extraction of singl ...
Modifying the chain-length selectivity of the
... three closely related lipases, which are from Burkholderia glumae (BGL), Burkholderia cepacia (BCL) and Pseudomonas aeruginosa (PAL), have typical funnel-like substrate-binding sites (Nobel et al., 1993; Kim et al., 1997; Nardini et al., 2000). In the case of BCL, it contains a binding pocket like a ...
... three closely related lipases, which are from Burkholderia glumae (BGL), Burkholderia cepacia (BCL) and Pseudomonas aeruginosa (PAL), have typical funnel-like substrate-binding sites (Nobel et al., 1993; Kim et al., 1997; Nardini et al., 2000). In the case of BCL, it contains a binding pocket like a ...
as a PDF
... as 11 triplet repeats (7 GAG, 3 AAG, and 1 CAG) that overlap the ycbK SD sequence and translation initiation region. Furthermore, the features of this operon suggested that it could be regulated by uncharged tRNATrp (35). yczA is preceded by a leader regulatory region with the characteristics of ope ...
... as 11 triplet repeats (7 GAG, 3 AAG, and 1 CAG) that overlap the ycbK SD sequence and translation initiation region. Furthermore, the features of this operon suggested that it could be regulated by uncharged tRNATrp (35). yczA is preceded by a leader regulatory region with the characteristics of ope ...
CBS (EC 4.2.1.22). The rate equation for the CBS reaction
... Concentrations of ATP, adenosine, betaine, dimethylglycine, glycine, NADPH, and serine, as well as a total concentration of all intracellular folates (F0) are assumed to be constant. In this way, either there is no dependence of reaction rates on these metabolites, or they are included in the releva ...
... Concentrations of ATP, adenosine, betaine, dimethylglycine, glycine, NADPH, and serine, as well as a total concentration of all intracellular folates (F0) are assumed to be constant. In this way, either there is no dependence of reaction rates on these metabolites, or they are included in the releva ...
Sense and Nonsense in the Genetic Code
... the lactose (14), tryptophan (13), histidine (15), and pyruvate dehydrogenase (16) clusters-that certain mutations, revertible by baseanalog mutagens, produce a pleiotropic mutant phenotype involving more than one of the genes in the cluster. There is polarity associated with the pleiotropic effect ...
... the lactose (14), tryptophan (13), histidine (15), and pyruvate dehydrogenase (16) clusters-that certain mutations, revertible by baseanalog mutagens, produce a pleiotropic mutant phenotype involving more than one of the genes in the cluster. There is polarity associated with the pleiotropic effect ...
mammalian hibernation: biochemical adaptation
... of ATP production needed to support their shivering is supplied by aerobic sugar oxidation in muscle using the massive stores of honey that the colony laid down from summer foraging. Hence, honeybees use a social solution to winter warmth. Another futile form of ATP turnover that supports thermogene ...
... of ATP production needed to support their shivering is supplied by aerobic sugar oxidation in muscle using the massive stores of honey that the colony laid down from summer foraging. Hence, honeybees use a social solution to winter warmth. Another futile form of ATP turnover that supports thermogene ...
FREE Solved Full Length Mock Test Paper
... Becaus e proteins can abs orb light maxim ally at 280 nm, they can be identified and quantified in s olution by using a s pectrophotom eter. Which of the following is true about the absorption of light by proteins ? (A) Proteins absorb infrared light. (B) All amino acids absorb light equally. (C) Th ...
... Becaus e proteins can abs orb light maxim ally at 280 nm, they can be identified and quantified in s olution by using a s pectrophotom eter. Which of the following is true about the absorption of light by proteins ? (A) Proteins absorb infrared light. (B) All amino acids absorb light equally. (C) Th ...
dbPSP: a curated database for protein phosphorylation sites in
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
... its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http:/ ...
Integrated Microbial Genomes
... Categories of metalloprotein and metalloenzymes functions The code: amino acids The Genomic Firehose ...
... Categories of metalloprotein and metalloenzymes functions The code: amino acids The Genomic Firehose ...
Channel Protein From Rat Heart Using Subtype
... of the primary sequence that demonstrate high homology among subtypes, it is not clear whether the unique characteristics of each subtype are due to differences in channel primary sequence, posttranslational modification, presence of associated f, and/or /32 subunits, or a combination of the above f ...
... of the primary sequence that demonstrate high homology among subtypes, it is not clear whether the unique characteristics of each subtype are due to differences in channel primary sequence, posttranslational modification, presence of associated f, and/or /32 subunits, or a combination of the above f ...
Journal of Biotechnology 84:
... binant constructions in the obtained clones. The presence of a foreign plasmid can produce a metabolic load in the host microorganism (Glick, 1995b). In this study, the growth of transconjugants with increased mps activity was evaluated in NB medium, in comparison with the strains without the recomb ...
... binant constructions in the obtained clones. The presence of a foreign plasmid can produce a metabolic load in the host microorganism (Glick, 1995b). In this study, the growth of transconjugants with increased mps activity was evaluated in NB medium, in comparison with the strains without the recomb ...
Hemoglobin Learning Objective Hemoglobin
... known as the MWC model, assumes that each subunit can exist in at least two conformational states and hypothesizes that all subunits make the transition from one state to the other simultaneously. According to this model, each subunit of a cooperatively binding protein is functionally identical. Bin ...
... known as the MWC model, assumes that each subunit can exist in at least two conformational states and hypothesizes that all subunits make the transition from one state to the other simultaneously. According to this model, each subunit of a cooperatively binding protein is functionally identical. Bin ...
Full-Text PDF
... substrate (Figure 5). In the PDB 1Q74 structure, the bound Zn2+ ion is pentacoordinate (three protein ligands and two water molecules), [4] while in the 4EWL structure the bound Zn2+ ion is tetrahedrally coordinated (4 ligands) [5]. These results likely suggest that the catalytic Zn2+ can easily swi ...
... substrate (Figure 5). In the PDB 1Q74 structure, the bound Zn2+ ion is pentacoordinate (three protein ligands and two water molecules), [4] while in the 4EWL structure the bound Zn2+ ion is tetrahedrally coordinated (4 ligands) [5]. These results likely suggest that the catalytic Zn2+ can easily swi ...
Constitutive expression of RyhB regulates the heme biosynthesis
... sdhCDAB site of the TCA cycle, resulting in the accumulation of succinate and glutamate in E. coli DALRA. To confirm it, we performed a qRT-PCR analysis. Among the genes analyzed in the TCA cycle, sdhB was the most affected (Fig. 4). The relative transcription level of sdhB was down-regulated to onl ...
... sdhCDAB site of the TCA cycle, resulting in the accumulation of succinate and glutamate in E. coli DALRA. To confirm it, we performed a qRT-PCR analysis. Among the genes analyzed in the TCA cycle, sdhB was the most affected (Fig. 4). The relative transcription level of sdhB was down-regulated to onl ...
Structure of L‑Serine Dehydratase from Legionella
... At least four types of Fe−S L-serine dehydratases that differ in their domain content and arrangement have been identified.17 They all contain a catalytic or α domain that harbors the binding site for the Fe−S cluster as well as a β domain whose function has not yet been completely defined. In the type ...
... At least four types of Fe−S L-serine dehydratases that differ in their domain content and arrangement have been identified.17 They all contain a catalytic or α domain that harbors the binding site for the Fe−S cluster as well as a β domain whose function has not yet been completely defined. In the type ...
Amino acid contents and biological value of protein in various
... 4.94 to 5.50, and in A. hypochondriacus from 4.68 to 5.62. Tryptophan levels varied from 1.36 to 1.70 in A. caudatus, 1.10 to 1.87 in A. cruentus, 1.19 to 1.79 in A. hypochondriacus, and 1.45 in A. hybridus. High levels of lysine, methionine and arginine in amaranth grains were demonstrated by Gorin ...
... 4.94 to 5.50, and in A. hypochondriacus from 4.68 to 5.62. Tryptophan levels varied from 1.36 to 1.70 in A. caudatus, 1.10 to 1.87 in A. cruentus, 1.19 to 1.79 in A. hypochondriacus, and 1.45 in A. hybridus. High levels of lysine, methionine and arginine in amaranth grains were demonstrated by Gorin ...
The Terminal Enzymes of Sialic Acid Metabolism: Acylneuraminate
... mutants lacking acylneuraminate pyruvate-lyase activity, the intracellular sialic acid concentration reaches toxic levels. Finally, there are also some microbial species with the lyase, which do not possess sialic acids, e.g., C. perfringens. Interestingly, these species often live in close contact ...
... mutants lacking acylneuraminate pyruvate-lyase activity, the intracellular sialic acid concentration reaches toxic levels. Finally, there are also some microbial species with the lyase, which do not possess sialic acids, e.g., C. perfringens. Interestingly, these species often live in close contact ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.