Amino acids
... among available a-keto acids. This permits synthesis of non-essential amino acids, using amino groups from other amino acids & carbon skeletons synthesized in a cell. Thus a balance of different amino acids is maintained, as proteins of varied amino acid contents are synthesized. Although the amino ...
... among available a-keto acids. This permits synthesis of non-essential amino acids, using amino groups from other amino acids & carbon skeletons synthesized in a cell. Thus a balance of different amino acids is maintained, as proteins of varied amino acid contents are synthesized. Although the amino ...
Unconstrained Structure Formation in Coarse
... operate at time- and length-scales that are currently unattainable by all-atom computer simulations. To cope with this difficulty, increasingly more accurate and sophisticated coarse-grained models are currently being developed. In the present thesis, we introduce a solvent-free coarse-grained model ...
... operate at time- and length-scales that are currently unattainable by all-atom computer simulations. To cope with this difficulty, increasingly more accurate and sophisticated coarse-grained models are currently being developed. In the present thesis, we introduce a solvent-free coarse-grained model ...
Formation of Monoterpenes in Antirrhinum majus
... Escherichia coli with and without a C-terminal polyhistidine (6xHis) tag extension (see Methods). The proteins thus expressed had no detectable prenyltransferase activity, even after partial purification (Figure 3A). Truncation of the signal peptides also did not yield functional prenyltransferases. ...
... Escherichia coli with and without a C-terminal polyhistidine (6xHis) tag extension (see Methods). The proteins thus expressed had no detectable prenyltransferase activity, even after partial purification (Figure 3A). Truncation of the signal peptides also did not yield functional prenyltransferases. ...
OptCDR: a general computational method for the design
... 2000) method and then expanded to include amino acids with similar chemical properties. When these sequence-based rules were not sufficient (25% of cases), the framework of the antibody variable region was aligned to a consensus framework and structural analysis was used to identify the CDRs. Throu ...
... 2000) method and then expanded to include amino acids with similar chemical properties. When these sequence-based rules were not sufficient (25% of cases), the framework of the antibody variable region was aligned to a consensus framework and structural analysis was used to identify the CDRs. Throu ...
Atlas of Antinuclear Antibodies
... 17. Brahms H, et al. A major, novel systemic lupus erythematosus autoantibody class recognizes the E, F, and G Sm snRNP proteins as an E-F-G complex but not in their denatured states. Arthritis Rheum. 40, 672-82, 1997 [PMID:9125249] 18. Fritzler MJ, Ali R, Tan EM. Antibodies from patients with mix ...
... 17. Brahms H, et al. A major, novel systemic lupus erythematosus autoantibody class recognizes the E, F, and G Sm snRNP proteins as an E-F-G complex but not in their denatured states. Arthritis Rheum. 40, 672-82, 1997 [PMID:9125249] 18. Fritzler MJ, Ali R, Tan EM. Antibodies from patients with mix ...
Full Text - the American Society of Animal Science
... was checked by dissociation curve analyses. Assuming that efficiencies of the target genes and 18S are the same, the amount of a specific target, normalized to an endogenous reference and relative to a calibrator (i.e., one sample from the low i.m. fat group) was calculated with the following formul ...
... was checked by dissociation curve analyses. Assuming that efficiencies of the target genes and 18S are the same, the amount of a specific target, normalized to an endogenous reference and relative to a calibrator (i.e., one sample from the low i.m. fat group) was calculated with the following formul ...
Structure based hypothesis of a mitochondrial
... was identified several years ago as the candidate gene for a human mitochondrial release factor [20]. More recently, it was shown that this protein does not show any capability of terminating translation at UAA or UAG stop codons and that mtRF1a is the actual release factor performing the translatio ...
... was identified several years ago as the candidate gene for a human mitochondrial release factor [20]. More recently, it was shown that this protein does not show any capability of terminating translation at UAA or UAG stop codons and that mtRF1a is the actual release factor performing the translatio ...
The Euglena gracilis chloroplast rpoB gene
... homologues to the E. coli /3 and /3'-subunits have been described (9, 10, 11, 12). It has been suggested that the genes for the chloroplast RNA polymerase are nuclear encoded (13). Subsequently, the equivalent of the E. coli rpoA (14), rpoB and rpoC (15) genes were reported in the spinach chloroplas ...
... homologues to the E. coli /3 and /3'-subunits have been described (9, 10, 11, 12). It has been suggested that the genes for the chloroplast RNA polymerase are nuclear encoded (13). Subsequently, the equivalent of the E. coli rpoA (14), rpoB and rpoC (15) genes were reported in the spinach chloroplas ...
Proteases of Senescing Oat Leaves
... All protein substrates tested were attacked by both enzymes, though at 6) at 0 C, and either used directly or concentrated by ultrafiltration strkingly different rates. Characterization of the digestion products, with in an Amicon cell equipped with a PM-10 membrane. As shown denatured hemogiobin as ...
... All protein substrates tested were attacked by both enzymes, though at 6) at 0 C, and either used directly or concentrated by ultrafiltration strkingly different rates. Characterization of the digestion products, with in an Amicon cell equipped with a PM-10 membrane. As shown denatured hemogiobin as ...
Computational protein design enables a novel one
... into multicarbon molecules such as fuels and other high value chemicals. Many synthetic pathways to produce value-added chemicals from common feedstocks, such as glucose, have been constructed in organisms that lack one-carbon anabolic pathways, such as Escherichia coli or Saccharomyces cerevisiae ( ...
... into multicarbon molecules such as fuels and other high value chemicals. Many synthetic pathways to produce value-added chemicals from common feedstocks, such as glucose, have been constructed in organisms that lack one-carbon anabolic pathways, such as Escherichia coli or Saccharomyces cerevisiae ( ...
Ultrasonic Absorption Mechanisms in Aqueous Solutions of Bovine
... the former, and not present in the latter, which may also possess relaxational behavior. A similar situation has been reported for bovine serum albumin.22 The composite ultrasonic absorption titration curve is shown in Figure 2 for the frequency range 8.9-50.5 MHz and over the pH range 1.5-12.3. Sim ...
... the former, and not present in the latter, which may also possess relaxational behavior. A similar situation has been reported for bovine serum albumin.22 The composite ultrasonic absorption titration curve is shown in Figure 2 for the frequency range 8.9-50.5 MHz and over the pH range 1.5-12.3. Sim ...
Ligand Binding and Allosteric Regulation
... communication between different binding sites by conformational changes). Used for O2 transport in red blood cell. – Hb is thus an allosteric protein. ...
... communication between different binding sites by conformational changes). Used for O2 transport in red blood cell. – Hb is thus an allosteric protein. ...
Abiotic stress in plants: Late Embryogenesis Abundant proteins Imen Amara
... transduction pathways biochemical modulation and proteomic machinery which lead to the survival or death of the affected plants (Ahuja I 2010; Xiong et al. 2002; Yamaguchi-Shinozaki and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classif ...
... transduction pathways biochemical modulation and proteomic machinery which lead to the survival or death of the affected plants (Ahuja I 2010; Xiong et al. 2002; Yamaguchi-Shinozaki and Shinozaki 2006). In Arabidopsis gene networks of stress-inducible genes have been identified and have been classif ...
SoyMilk - Soyfoods Association of North America
... Use 1-1/2 cups regular soymilk in place of 2 cups cow’s milk when making instant pudding (4-serving size), but the dessert may still be a little thinner than standard pudding. Pour your choice of regular, reduced-fat or non-fat soymilk over cereal, and add sliced fruit. Enjoy a glass of soy eggnog-s ...
... Use 1-1/2 cups regular soymilk in place of 2 cups cow’s milk when making instant pudding (4-serving size), but the dessert may still be a little thinner than standard pudding. Pour your choice of regular, reduced-fat or non-fat soymilk over cereal, and add sliced fruit. Enjoy a glass of soy eggnog-s ...
PDF
... protein localization to plastids was based on the presence of signal peptide motifs in combination with plastid localization motifs previously shown to be required in diatoms. We identified genes potentially involved in a C4-like photosynthesis in P. tricornutum and, on the basis of sequence-based p ...
... protein localization to plastids was based on the presence of signal peptide motifs in combination with plastid localization motifs previously shown to be required in diatoms. We identified genes potentially involved in a C4-like photosynthesis in P. tricornutum and, on the basis of sequence-based p ...
7.3 Translation (HL ONLY)
... • Bound ribosomes synthesize proteins primarily for secretion or for use in lysosomes. • Translation can occur immediately after transcription in prokaryotes due to the absence of a nuclear membrane. ...
... • Bound ribosomes synthesize proteins primarily for secretion or for use in lysosomes. • Translation can occur immediately after transcription in prokaryotes due to the absence of a nuclear membrane. ...
Allosteric pathways in imidazole glycerol phosphate synthase
... products enter the histidine and purine biosynthetic pathways, respectively. IGPS is not present in mammals, but it is involved in essential biosynthetic pathways of microorganisms. In particular, many plant pathogens, and importantly opportunistic human pathogens such as Cryptococcus, Candida, and ...
... products enter the histidine and purine biosynthetic pathways, respectively. IGPS is not present in mammals, but it is involved in essential biosynthetic pathways of microorganisms. In particular, many plant pathogens, and importantly opportunistic human pathogens such as Cryptococcus, Candida, and ...
CBS domains: structure, function, and pathology in human proteins
... CBS domains can adopt the typical (1)-␣1-2-3-␣2 core structure and form an intramolecular CBS pair. It should be noted that in the bacterial proteins the CBS pairs are always formed by intramolecular association of two CBS domains. Although at this time one cannot formally exclude the formation o ...
... CBS domains can adopt the typical (1)-␣1-2-3-␣2 core structure and form an intramolecular CBS pair. It should be noted that in the bacterial proteins the CBS pairs are always formed by intramolecular association of two CBS domains. Although at this time one cannot formally exclude the formation o ...
Marinus Pilon, Ph - Colorado State University
... various parts of the transit peptide. FEBS lett. 358, 39-42. Bulychev A, Pilon M, Dassen, H, van 't Hof R, Vredenburg W, and de Kruijff B (1995) Precursor-mediated opening of translocation pores in chloroplast envelopes. FEBS Lett. 356, 204-206. Pilon M, America T, van 't Hof R, de Kruijff B, and We ...
... various parts of the transit peptide. FEBS lett. 358, 39-42. Bulychev A, Pilon M, Dassen, H, van 't Hof R, Vredenburg W, and de Kruijff B (1995) Precursor-mediated opening of translocation pores in chloroplast envelopes. FEBS Lett. 356, 204-206. Pilon M, America T, van 't Hof R, de Kruijff B, and We ...
Planta - University of Regina
... instructions. Following RNase H treatment, the resulting singlestrand cDNA mixture was used as a template for the polymerase chain reaction (PCR). For PCR ampli®cation of the core region of the cDNA encoding CHS-like enzymes, two sets of degenerate oligonucleotide primers (see Fig. 2) were synthesiz ...
... instructions. Following RNase H treatment, the resulting singlestrand cDNA mixture was used as a template for the polymerase chain reaction (PCR). For PCR ampli®cation of the core region of the cDNA encoding CHS-like enzymes, two sets of degenerate oligonucleotide primers (see Fig. 2) were synthesiz ...
Genomics Insights esTs from seeds to Assist the selective Breeding
... particularly interesting for the identification of cDNAs involved in fatty acid biosynthesis through EST profiling.19 In addition, EST sequencing, associated with real-time PCR and even pyrosequencing,20 allows the investigation of the gene regulation network both in the lipid pathway21–23 and amon ...
... particularly interesting for the identification of cDNAs involved in fatty acid biosynthesis through EST profiling.19 In addition, EST sequencing, associated with real-time PCR and even pyrosequencing,20 allows the investigation of the gene regulation network both in the lipid pathway21–23 and amon ...
amino acids
... have a variety of roles in metabolism. – One particularly important function is as the building blocks of proteins – forming parts of coenzymes – as precursors for the biosynthesis of molecules such as heme ...
... have a variety of roles in metabolism. – One particularly important function is as the building blocks of proteins – forming parts of coenzymes – as precursors for the biosynthesis of molecules such as heme ...
Enzymes of the Calvin Cycle and Intermediary
... showed significantly greater activities at this dilution rate compared with other steadystate cultures (Table 1). DISCUSSION ...
... showed significantly greater activities at this dilution rate compared with other steadystate cultures (Table 1). DISCUSSION ...
NSC 108 - National Open University of Nigeria
... This course is thought as Medical Biochemistry; medical biochemistry is a subset of general biochemistry. In this course, you will learn the biochemical activities that occur inside the cell and how these activities determine our state of health and what happens in disease. The reason why we fall si ...
... This course is thought as Medical Biochemistry; medical biochemistry is a subset of general biochemistry. In this course, you will learn the biochemical activities that occur inside the cell and how these activities determine our state of health and what happens in disease. The reason why we fall si ...
ANN 303 PRINCIPLES OF ANIMAL NUTRITION (A)
... acid with the liberation of ammonia. De-amination is therefore a process in which the amino grouyp of amino acid is removed and keto acid is formed with the liberation of ammonia. Keto acid formed from amino acid can be oxidized for energy supply or used for glucose synthesis or converted to fat. Fr ...
... acid with the liberation of ammonia. De-amination is therefore a process in which the amino grouyp of amino acid is removed and keto acid is formed with the liberation of ammonia. Keto acid formed from amino acid can be oxidized for energy supply or used for glucose synthesis or converted to fat. Fr ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.