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Bioinformatics Toolbox
Bioinformatics Toolbox

... explore the data (phytreetool or view). This GUI also allows you to prune branches, reorder, rename, and explore distances. Phylogenetic tree object methods — You can access the functionality of the phytreetool GUI using methods for a phylogenetic tree object (phytree). Get property values (get) and ...
The different roles of tryptophan transfer RNA in regulating trp
The different roles of tryptophan transfer RNA in regulating trp

Gluconeogenesis
Gluconeogenesis

... Gluconeogenesis is not just the reverse of glycolysis Several steps are different so that control of one pathway does not inactivate the other. However many steps are the same. Three steps are different from glycolysis. 1 Pyruvate to PEP 2 Fructose 1,6- bisphosphate to Fructose-6phosphate 3 Glucose ...
Neutralizing scFv Antibodies against Infectious Bursal Disease Virus
Neutralizing scFv Antibodies against Infectious Bursal Disease Virus

... membrane anchoring and compatible RE sites with the cloning vector was constructed from expression vector pET27b. The VH and VL gene pool against IBDV were ampli ied by PCR from the cDNA. The vector irst cleaved with HindⅢ and NheI and ligated with the VH fragment and then cleaved with BamHI and Xho ...
eg1
eg1

... multiple forms (Knowles et al. 1987, Kubicek 1992). V. volvacea produces a cellulolytic system that includes multiple forms of all three classes when grown on crystalline cellulose (Cai et al. 1994, 1999). In addition to EG1, four other CMC-hydrolysing proteins were separated in lower yields from cu ...
Plasma membrane
Plasma membrane

... Cilium. A cilium contains nine pairs of microtubules surrounding a central pair (9  2 array). The basal body to which the cilium is anchored has a structure similar to that of a centriole. ...
2.3.3 Protein and amino acid metabolism
2.3.3 Protein and amino acid metabolism

... by covalent modification. The enzyme can exist in both dimeric and tetrameric forms. Glucagon activates the enzyme by means of protein kinase A phosphorylation. In addition to its role as a substrate, phenylalanine promotes its conversion to the more active, tetrameric form. Both types of activation ...
Study of the distribution of autotrophic CO2 fixation
Study of the distribution of autotrophic CO2 fixation

Molecular cloning of a rhodopsin gene from salamander rods.
Molecular cloning of a rhodopsin gene from salamander rods.

... been cloned and sequenced from the larval tiger salamander, Ambystoma tigrinum. This cDNA encodes 354 amino acids that, by hydropathy profile, could form seven transmembrane domains characteristic of other rhodopsins. Sequence identity was found with other amphibian rhodopsins at the nucleic acid (8 ...
Molecular Characterisation of the 76 kDa Iron
Molecular Characterisation of the 76 kDa Iron

... preceded by an in-frame stop codon (*). The locations of PCR primers and Hindi restriction sites used in the investigation, as well as a potential polyadenylation signal (AATAAG) with an upstream GT-rich region (24 bp, 88% G or T) is denoted on top. The protein has a cleaved presequence (white box) ...
A Molecular Basis for Multiple Herbicide Resistance in Black
A Molecular Basis for Multiple Herbicide Resistance in Black

... •Recombinant AmGSTF1 and C120V mutant independently expressed as Strep II tag fusions. • In vitro inhibition of activity towards CDNB. ...
Bil 255 – CMB
Bil 255 – CMB

... – one enzyme with 2 substrates with following Km's - 0.1 M & 0.05 M one takes more substrate to reach same rate…  Vmax – many enzymes have individual steps in a complex reaction sequences, each with their own Km's….. ...
Molecular insights into RNA and DNA helicase evolution from the
Molecular insights into RNA and DNA helicase evolution from the

Molecular insights into mitochondrial transcription and its
Molecular insights into mitochondrial transcription and its

... The mitochondrion is an organelle of the eukaryotic cell responsible for the production of most of the cellular energy-carrying molecule adenosine triphosphate (ATP), through the process of oxidative phosphorylation. The mitochondrion contains its own genome, a small circular DNA molecule (mtDNA), e ...
Amino Acids - Rose
Amino Acids - Rose

Plasma membrane
Plasma membrane

... The hydrogen atoms are delivered to enzymes and coenzymes of the cristae which catalyze the synthesis of ATP from ADP and phosphate. At the end of this process, oxygen combines with the hydrogen atoms to form ...
Computational Biology
Computational Biology

... Adamian & Liang (2006): discuss failures ...
PROTEIN SUBCELLULAR LOCALIZATION
PROTEIN SUBCELLULAR LOCALIZATION

Chapter 3 powerpoint File - District 196 e
Chapter 3 powerpoint File - District 196 e

... The hydrogen atoms are delivered to enzymes and coenzymes of the cristae which catalyze the synthesis of ATP from ADP and phosphate. At the end of this process, oxygen combines with the hydrogen atoms to form ...
Attachment 2 - Food Standards Australia New Zealand
Attachment 2 - Food Standards Australia New Zealand

... sequence. Polymerase chain reaction (PCR) analyses were performed to verify the sequences at the 5' and 3' ends of the insert. Insert and copy number Southern hybridisation was used to determine the number and nature of DNA insertions in line H7-1. The genomic DNA was digested and probed with an int ...
Dynamic Programming
Dynamic Programming

... The first four sequences possibly derive from closely related species and the last three from three more distant species. Since A occurs with high frequency in the first four sequences, the observed number of pairings of A with A will be higher than is appropriate if we are comparing more distantly ...
Lecture 1 - "Hudel" Luecke
Lecture 1 - "Hudel" Luecke

... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
Chapter 5
Chapter 5

... Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings ...
File
File

... A) are used to form amino acids. B) form between fatty acids. C) are formed by a hydrolysis reaction. D) link amino acids. Answer: D Topic: 3.11 Skill: Knowledge/Comprehension 46) Which of the following statements about enzymes is false? A) They increase the rate of chemical reactions. B) They funct ...
Tetrahydrobiopterin and its functions
Tetrahydrobiopterin and its functions

... antiparallel β-barrel with a diameter of 35 Å. The decamer is formed by a face-to-face association of two pentamers whereby the antiparallel helix pair α2/α3 of one monomer is intertwined with that of another monomer. The decamer has a toroidal shape with an approximate height of 65 Å and a diameter ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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