Tetrahydrobiopterin and its functions
... antiparallel β-barrel with a diameter of 35 Å. The decamer is formed by a face-to-face association of two pentamers whereby the antiparallel helix pair α2/α3 of one monomer is intertwined with that of another monomer. The decamer has a toroidal shape with an approximate height of 65 Å and a diameter ...
... antiparallel β-barrel with a diameter of 35 Å. The decamer is formed by a face-to-face association of two pentamers whereby the antiparallel helix pair α2/α3 of one monomer is intertwined with that of another monomer. The decamer has a toroidal shape with an approximate height of 65 Å and a diameter ...
Classification of pseudo pairs between nucleotide bases and amino
... Both uracil and thymine pair with Asn and Gln in two different geometries at their Watson–Crick edges. In both pseudo-pair geometries, the O atom of CONH2 makes a hydrogen bond with N3–H. On the other hand, the amino group NH2 can interact either with O2 or O4. However, no U-Gln pseudo pair with O2( ...
... Both uracil and thymine pair with Asn and Gln in two different geometries at their Watson–Crick edges. In both pseudo-pair geometries, the O atom of CONH2 makes a hydrogen bond with N3–H. On the other hand, the amino group NH2 can interact either with O2 or O4. However, no U-Gln pseudo pair with O2( ...
Biochimie
... This review covers the recent discoveries in the lipid metabolism of eukaryotic photosynthetic microalgae, an extremely diverse group of unicellular organisms dwelling in various environments. Our goal is to cover some aspects which have not been described in previous comprehensive reviews on algal ...
... This review covers the recent discoveries in the lipid metabolism of eukaryotic photosynthetic microalgae, an extremely diverse group of unicellular organisms dwelling in various environments. Our goal is to cover some aspects which have not been described in previous comprehensive reviews on algal ...
Development of Software Package for Determining Protein
... Removing unfavorable van der Waals clashes (when two atoms try to occupy the same space) Assigning charge ( partial or whole) and van der Waals radii parameters from a variety of force fields ...
... Removing unfavorable van der Waals clashes (when two atoms try to occupy the same space) Assigning charge ( partial or whole) and van der Waals radii parameters from a variety of force fields ...
The Role of the Bundle Sheath in the Leaf Development of C3 plants
... remained elusive. Hence, we aimed at genetically cloning the affected gene. The gene was mapped to encode for ATase2, the first enzyme in de novo purine biosynthesis. The effect of the purine-derived phytohormone cytokinin on plant performance was assessed by growth kinetic analysis, cytokinin profi ...
... remained elusive. Hence, we aimed at genetically cloning the affected gene. The gene was mapped to encode for ATase2, the first enzyme in de novo purine biosynthesis. The effect of the purine-derived phytohormone cytokinin on plant performance was assessed by growth kinetic analysis, cytokinin profi ...
NMR studies of the methionine methyl groups in calmodulin
... [3]. NMR and X-ray studies of complexes of CaM with various CaM-binding domain peptides show that all the Met residues, with the possible exception of Met-76, are in contact with the hydrophobic face(s) of the bound peptides [8-12]. Oxidation studies of CaM's Met residues have revealed that all of t ...
... [3]. NMR and X-ray studies of complexes of CaM with various CaM-binding domain peptides show that all the Met residues, with the possible exception of Met-76, are in contact with the hydrophobic face(s) of the bound peptides [8-12]. Oxidation studies of CaM's Met residues have revealed that all of t ...
pdf-1 - The Wolfson Centre for Applied Structural Biology
... IgG, a monomer, is the predominant Ig class present in human serum. Produced as part of the secondary immune response to an antigen, this class of immunoglobulin constitutes approximately 75% of total serum Ig. IgG is the only class of Ig that can cross the placenta in humans, and it is largely resp ...
... IgG, a monomer, is the predominant Ig class present in human serum. Produced as part of the secondary immune response to an antigen, this class of immunoglobulin constitutes approximately 75% of total serum Ig. IgG is the only class of Ig that can cross the placenta in humans, and it is largely resp ...
BIOCHEMISTRY
... Biochemistry or Biological chemistry is the branch of knowledge that deals with the structure of chemical compounds that make up part of living matter, their transformations and physico-chemical processes that constitute the basis of vital activity. Biochemistry is a part of Biology; it encompasses ...
... Biochemistry or Biological chemistry is the branch of knowledge that deals with the structure of chemical compounds that make up part of living matter, their transformations and physico-chemical processes that constitute the basis of vital activity. Biochemistry is a part of Biology; it encompasses ...
Neonatal Parenteral Nutrition - UCSF Benioff Children`s Hospital
... >2 grams protein/kg/day. These guidelines may be used for both the preterm and term infant, although the term infant may not need the concentrated breastmilk/formula. Directions: Determine total fluid allowance for the day. Write the TPN for the suggested volume and substrate amounts as indicated by ...
... >2 grams protein/kg/day. These guidelines may be used for both the preterm and term infant, although the term infant may not need the concentrated breastmilk/formula. Directions: Determine total fluid allowance for the day. Write the TPN for the suggested volume and substrate amounts as indicated by ...
Genetic engineering of lactic acid bacteria to produce
... pure L(+)-lactic acid. Naturally, this organism produces mixture of D(-)- and L(+)-lactic acid during fermentation of sugars. Production of D(-)- and L(+)-lactic acid is based on the activity of LdhD and LdhL, respectively. In this work, LdhD enzyme activity was removed by using two different geneti ...
... pure L(+)-lactic acid. Naturally, this organism produces mixture of D(-)- and L(+)-lactic acid during fermentation of sugars. Production of D(-)- and L(+)-lactic acid is based on the activity of LdhD and LdhL, respectively. In this work, LdhD enzyme activity was removed by using two different geneti ...
Butyrate formation from glucose by the rumen protozoon Dasytricha
... Production of butyrate by the holotrich protozoon Dasytricha ruminantium involves the enzymes of glycolysis, pyruvate: ferredoxin oxidoreductase, acetyl-CoA: acetylCoA C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, 3-hydroxyacyl-CoA hydro-lyase, 3-hydroxyacyl-CoA reductase, phosphate butyr ...
... Production of butyrate by the holotrich protozoon Dasytricha ruminantium involves the enzymes of glycolysis, pyruvate: ferredoxin oxidoreductase, acetyl-CoA: acetylCoA C-acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, 3-hydroxyacyl-CoA hydro-lyase, 3-hydroxyacyl-CoA reductase, phosphate butyr ...
Characterization of somatic antigens of adult
... rTES-30USM) [13]. Presently a Dot-ELISA test for toxocarosis is under evaluation which is considered as a reliable one as it presents many advantages as a basic diagnostic test. For example, it is highly stable, it does not require specialised tools to analyse the results, it has a lower cost and it ...
... rTES-30USM) [13]. Presently a Dot-ELISA test for toxocarosis is under evaluation which is considered as a reliable one as it presents many advantages as a basic diagnostic test. For example, it is highly stable, it does not require specialised tools to analyse the results, it has a lower cost and it ...
Adipocyte metabolic pathways regulated by diet control
... enzymes promote GSC maintenance. Finally, carrier proteins involved in the transport of lipids and iron ...
... enzymes promote GSC maintenance. Finally, carrier proteins involved in the transport of lipids and iron ...
nitrogen assimilation and global regulation in escherichia coli
... that glutamate starvation prevents Ntr induction (35). A mechanism by which this occurs is not stated. A hybrid hypothesis is that glutamine accumulation prevents Ntr gene expression until glutamate starvation stops metabolism and growth. In any case, the arguments are complex and subject to several ...
... that glutamate starvation prevents Ntr induction (35). A mechanism by which this occurs is not stated. A hybrid hypothesis is that glutamine accumulation prevents Ntr gene expression until glutamate starvation stops metabolism and growth. In any case, the arguments are complex and subject to several ...
Engineering subunit association of multisubunit proteins
... the high hydrophobicity of the dimer–dimer interface, which would be exposed to solvent in a dimeric streptavidin, suggests that the resulting molecule would have very low solubility in aqueous media. In agreement with the calculations, a streptavidin containing the H127D mutation formed insoluble a ...
... the high hydrophobicity of the dimer–dimer interface, which would be exposed to solvent in a dimeric streptavidin, suggests that the resulting molecule would have very low solubility in aqueous media. In agreement with the calculations, a streptavidin containing the H127D mutation formed insoluble a ...
The Structure of the PanD/PanZ Protein Complex
... of enzymes have been shown to be enhanced by accessory proteins (McDonald et al., 1998), although the structural basis for such enhancement has not been determined. Since the first identification of pyruvoyl-dependent enzymes (van Poelje and Snell, 1990; Williamson and Brown, 1979), it has been wide ...
... of enzymes have been shown to be enhanced by accessory proteins (McDonald et al., 1998), although the structural basis for such enhancement has not been determined. Since the first identification of pyruvoyl-dependent enzymes (van Poelje and Snell, 1990; Williamson and Brown, 1979), it has been wide ...
PROTEIN PHOSPHORYLATION AND CELLULAR REGULATION, I by
... transmission of signals that impinge on cells. This is true not only with respect to the transduction of hormone and growth factor signals but for other types of stimuli as well. For example, as mentioned earlier, electrical stimulation of muscle leads to changes in protein phosphorylation within th ...
... transmission of signals that impinge on cells. This is true not only with respect to the transduction of hormone and growth factor signals but for other types of stimuli as well. For example, as mentioned earlier, electrical stimulation of muscle leads to changes in protein phosphorylation within th ...
The codon adaptation index-a measure of directional synonymous
... category are better than others. Third, there is no good basis for conparison between species because the proportional division of the codon table into the two categories may differ; e.g., Ikemura (1) identified 21 optimal codons for 14 amino acids in E.coll. and 19 optimal codons for 13 amino acids ...
... category are better than others. Third, there is no good basis for conparison between species because the proportional division of the codon table into the two categories may differ; e.g., Ikemura (1) identified 21 optimal codons for 14 amino acids in E.coll. and 19 optimal codons for 13 amino acids ...
Early days of tRNA research: Discovery, function, purification and
... intermediate carrier of the amino acid in protein synthesis (table 1). Independently, Ogata and Nohara (1957) obtained evidence for transfer of amino acid by the pH 5 enzyme fraction to an RNA. At the same time, Holley (1957) showed that the pH 5 enzyme catalyzed alanine-dependent ATP-AMP exchange t ...
... intermediate carrier of the amino acid in protein synthesis (table 1). Independently, Ogata and Nohara (1957) obtained evidence for transfer of amino acid by the pH 5 enzyme fraction to an RNA. At the same time, Holley (1957) showed that the pH 5 enzyme catalyzed alanine-dependent ATP-AMP exchange t ...
PREDICTION OF THE INTERACTION OF HIV
... integration of the double-stranded retroviral DNA into the genome of the host cell. HIV-1 integrase, the enzyme that inserts the vital DNA into the host chromosome, is an attractive and rational target for anti-AIDS drug design because it is essential for HIV replication and there are no known count ...
... integration of the double-stranded retroviral DNA into the genome of the host cell. HIV-1 integrase, the enzyme that inserts the vital DNA into the host chromosome, is an attractive and rational target for anti-AIDS drug design because it is essential for HIV replication and there are no known count ...
Studier`s autoinduction media
... auxotroph B834(DE3) or the prototroph BL21(DE3). The presence of Se-Met reduces the growth rate of the two strains comparably, presumably because both strains incorporate Se-Met into their proteins in place of methionine (but possibly due to other toxic effects as well). Enzymes of the methionine sy ...
... auxotroph B834(DE3) or the prototroph BL21(DE3). The presence of Se-Met reduces the growth rate of the two strains comparably, presumably because both strains incorporate Se-Met into their proteins in place of methionine (but possibly due to other toxic effects as well). Enzymes of the methionine sy ...
Ribosomes of Mouse Liver following
... while membranous material is trapped at the interface of the sucrose layers. It has been shown (31) and confirmed in this work (Table 4) that following the 1st centrifugation for sedimentation of free ribosomes some free monomers remain in the overlying 2.0 M sucrose cushion. Therefore, this cushion ...
... while membranous material is trapped at the interface of the sucrose layers. It has been shown (31) and confirmed in this work (Table 4) that following the 1st centrifugation for sedimentation of free ribosomes some free monomers remain in the overlying 2.0 M sucrose cushion. Therefore, this cushion ...
Molecular cloning of a cDNA encoding a novel Ca2+
... Fig. 5. The TB-D plate assay of nuclease activities. To make an expression construct of the cloned cDNA under the control of the lac promoter, the 5P region upstream from the EcoRI site (see Fig. 1) in the cDNA to the EcoRI site in the vector was removed by EcoRI digestion and subsequently self-liga ...
... Fig. 5. The TB-D plate assay of nuclease activities. To make an expression construct of the cloned cDNA under the control of the lac promoter, the 5P region upstream from the EcoRI site (see Fig. 1) in the cDNA to the EcoRI site in the vector was removed by EcoRI digestion and subsequently self-liga ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.